Yr Athro Vincenzo Crunelli

Athro Emeritws

Ysgol y Biowyddorau

: Ar gael fel goruchwyliwr ôl-raddedig

Mae fy ymchwil yn canolbwyntio ar y mecanweithiau cellog a rhwydwaith sy'n gweithredu yn y thalamws a'r cortex yn ystod epilepsi cwsg ac absenoldeb, gan ganolbwyntio ar gynulliadau niwronol ac astrocytig. Mae fy grŵp amlddisgyblaethol yn defnyddio cyfuniad o dechnegau microsgopig sganio laser electroffisiolegol, morffolegol, imiwnocemegol, cyfrifiadol a 2-photon mewn anifeiliaid arferol a modelau trawsgenig. Mae'r technegau hyn yn cael eu cymhwyso in vitro (diwylliant sylfaenol, sleisys ymennydd), yn ogystal ag mewn paratoadau anaestheteiddio ac yn ymddwyn yn rhydd. Rydym hefyd yn defnyddio sbectrosgopeg MRI i fesur niwrodrosglwyddyddion a'u metabolion yn ymennydd plant a phobl ifanc ag epilepsi absennol.

Rwy'n gysylltiedig â:

Sefydliad Ymchwil Niwrowyddoniaeth ac Iechyd Meddwl (https://www.cardiff.ac.uk/neuroscience-mental-health)

Rhwydwaith Cysgu Caerdydd (https://www.cardiff.ac.uk/research/explore/research-units/cardiff-sleep-network)

Oes gennych chi ddiddordeb mewn ymuno â'm labordy fel myfyriwr ôl-raddedig hunan-ariannu neu bostdoc/cymrodyr? Cysylltwch â mi drwy e-bost.

Date
Type

2021

Deidda, G., Pierucci, M., Crunelli, V. and Di Giovanni, G. 2021. 5-HT/GABA interaction in neurodevelopment and plasticity. In: Progress in Brain Research., Vol. 259. ELSEVIER SCIENCE BV, pp. 287-317., (10.1016/bs.pbr.2021.01.009)
Iacone, Y. et al. 2021. Systemic administration of ivabradine, a hyperpolarization-activated cyclic nucleotide-gated channel inhibitor, blocks spontaneous absence seizures. Epilepsia 62(7), pp. 1729-1743. (10.1111/epi.16926)
Deidda, G., Crunelli, V. and Di Giovanni, G. 2021. 5-HT/GABA interaction in epilepsy. In: Progress in Brain Research., Vol. 259. ELSEVIER, pp. 265-286., (10.1016/bs.pbr.2021.01.008)

2020

Crunelli, V., Lorincz, M. L., McCafferty, C., Regis, L. C., Leresche, N., Di Giovanni, G. and David, F. 2020. Clinical and experimental insight in pathophysiology, comorbidity and therapy of absence seizures. Brain 143(8), pp. 2341-2368. (10.1093/brain/awaa072)
Davis, B., David, F., O’Regan, C., Adam, M., Harwood, A., Crunelli, V. and Isles, A. 2020. Impairments in sensory-motor gating and information processing in a mouse model of Ehmt1 haploinsufficiency. Brain and Neuroscience Advances 4 (10.1177/2398212820928647)
Armada-Moreira, A. et al. 2020. Going the extra (synaptic) mile: excitotoxicity as the road toward neurodegenerative diseases. Frontiers in Cellular Neuroscience 14, article number: 90. (10.3389/fncel.2020.00090)
Xapelli, S., Diógenes, M. J., Crunelli, V., Fitzsimons, C. P. and Vaz, S. H. 2020. Editorial: glial and neural stem cells as new therapeutic targets for neurodegenerative disorders. Frontiers in Cellular Neuroscience 14, article number: 71. (10.3389/fncel.2020.00071)

2019

Corney, B., Widnall, C., Rees, D., Davies, J., Crunelli, V. and Carter, D. 2019. Regulatory architecture of the neuronal Cacng2/Tarpγ2 gene promoter: multiple repressive domains, a polymorphic regulatory short tandem repeat, and bidirectional organization with co-regulated lncRNAs. Journal of Molecular Neuroscience 67(2), pp. 282-294. (10.1007/s12031-018-1208-x)

2018

Baruchin, L. J., Ranson, A., Good, M. and Crunelli, V. 2018. Absence of neuronal response modulation with familiarity in perirhinal cortex. Neuroscience 394, pp. 23-29. (10.1016/j.neuroscience.2018.10.020)
McCafferty, C., Connelly, W. M., Celli, R., Ngomba, R. T., Nicoletti, F. and Crunelli, V. 2018. Genetic rescue of absence seizures. CNS Neuroscience & Therapeutics 24(8), pp. 745-758. (10.1111/cns.12858)
David, F. et al. 2018. Suppression of HCN channel function in thalamocortical neurons prevents genetically determined and pharmacologically induced absence seizures. Journal of Neuroscience 38(30), pp. 6615-6627. (10.1523/JNEUROSCI.0896-17.2018)
Di Giovanni, G., Crunelli, V. and Rizzolatti, G. 2018. Editorial: Interdisciplinary approaches for neuropathology. CNS Neuroscience & Therapeutics 24(8), pp. 665-668. (10.1111/cns.13004)
Bombardi, C., Venzi, M., Crunelli, V. and Di Giovanni, G. 2018. Developmental changes of GABA immunoreactivity in cortico-thalamic networks of an absence seizure model. Neuropharmacology 136(Part A), pp. 56-67. (10.1016/j.neuropharm.2018.01.047)
McCafferty, C. et al. 2018. Cortical drive and thalamic feed-forward inhibition control thalamic output synchrony during absence seizures. Nature Neuroscience 21, pp. 744-756. (10.1038/s41593-018-0130-4)
Crunelli, V. et al. 2018. Dual function of thalamic low-vigilance state oscillations: rhythm-regulation and plasticity. Nature Reviews Neuroscience 19, pp. 107-118. (10.1038/nrn.2017.151)

2017

Connelly, W. M., Crunelli, V. and Errington, A. C. 2017. Variable action potential backpropagation during tonic firing and low-threshold spike bursts in thalamocortical but not thalamic reticular nucleus neurons. Journal of Neuroscience 37(21), pp. 5319-5333. (10.1523/JNEUROSCI.0015-17.2017)

2016

Guo, J. N. et al. 2016. Impaired consciousness in patients with absence seizures investigated by functional MRI, EEG, and behavioural measures: a cross-sectional study. Lancet Neurology 15(13), pp. 1336-1345. (10.1016/S1474-4422(16)30295-2)
Venzi, M., David, F., Bellet, J., Cavaccini, A., Bombardi, C., Crunelli, V. and Di Giovanni, G. 2016. Role for serotonin2A (5-HT2A) and 2C (5-HT2C) receptors in experimental absence seizures. Neuropharmacology 108, pp. 292-304. (10.1016/j.neuropharm.2016.04.016)
Connelly, W. M., Crunelli, V. and Errington, A. C. 2016. Passive synaptic normalization and input synchrony-dependent amplification of cortical feedback in thalamocortical neuron dendrites. Journal of Neuroscience 36(13), pp. 3735-3754. (10.1523/JNEUROSCI.3836-15.2016)
David, F., Crunelli, V., Leresche, N. and Lambert, R. C. 2016. Dynamic Analysis of the Conditional Oscillator Underlying Slow Waves in Thalamocortical Neurons. Frontiers in Neural Circuits 10, pp. 1-11., article number: 10. (10.3389/fncir.2016.00010)
Avoli, M., Crunelli, V. and Jefferys, J. 2016. Preface for methods and models in epilepsy research. Journal of Neuroscience Methods 260, pp. 1. (10.1016/j.jneumeth.2015.11.016)
Lidster, K. et al. 2016. Opportunities for improving animal welfare in rodent models of epilepsy and seizures. Journal of Neuroscience Methods 260, pp. 2-25. (10.1016/j.jneumeth.2015.09.007)
Connelly, W., Laing, M., Errington, A. C. and Crunelli, V. 2016. The thalamus as a low pass filter: filtering at the cellular level does not equate with filtering at the network level. Frontiers in Neural Circuits 9, article number: 89. (10.3389/fncir.2015.00089)

2015

Crunelli, V. and Di Giovanni, G. 2015. Differential control by 5-HT and 5-HT1A, 2A, 2C receptors of phasic and tonic GABAA inhibition in the visual thalamus [Letter]. CNS Neuroscience & Therapeutics 21(12), pp. 967-970. (10.1111/cns.12480)
Connelly, W. M., Crunelli, V. and Errington, A. C. 2015. The global spike: conserved dendritic properties enable unique Ca2+ spike generation in low-threshold spiking neurons. Journal of Neuroscience 35(47), pp. 15505-15522. (10.1523/JNEUROSCI.2740-15.2015)
Griemsmann, S. et al. 2015. Characterization of panglial gap junction networks in the thalamus, neocortex, and hippocampus reveals a unique population of glial cells. Cerebral Cortex 25(10), pp. 3420-3433. (10.1093/cercor/bhu157)
Lorincz, M. L., Gunner, D., Bao, Y., Connelly, W. M., Isaac, J. T. R., Hughes, S. W. and Crunelli, V. 2015. A distinct class of slow (~0.2–2 Hz)) intrinsically bursting layer 5 pyramidal neurons determines UP/DOWN state dynamics in the neocortex. Journal of Neuroscience 35(14), pp. 5442-5458. (10.1523/JNEUROSCI.3603-14.2015)
Crunelli, V., David, F., Lorincz, M. L. and Hughes, S. W. 2015. The thalamocortical network as a single slow wave-generating unit. Current Opinion in Neurobiology 31, pp. 72-80. (10.1016/j.conb.2014.09.001)
Venzi, M., Di Giovanni, G. and Crunelli, V. 2015. A critical evaluation of the Gamma-Hydroxybutyrate (GHB) model of absence seizures. CNS Neuroscience & Therapeutics 21(2), pp. 123-140. (10.1111/cns.12337)
Crunelli, V., Carmignoto, G. and Steinhauser, C. 2015. Novel astrocyte targets: New avenues for the therapeutic treatment of epilepsy. The Neuroscientist 21(1), pp. 62-83. (10.1177/1073858414523320)

2014

Gould, T. M., Chen, L., Emri, Z., Pirttimaki, T., Errington, A. C., Crunelli, V. and Parri, H. R. 2014. GABAB receptor-mediated activation of astrocytes by gamma-hydroxybutyric acid. Philosophical Transactions of the Royal Society of London Series B - Biological Sciences 369(1654), article number: 20130607. (10.1098/rstb.2013.0607)
Taylor, H. L. et al. 2014. Investigating local and long-range neuronal network dynamics by simultaneous optogenetics, reverse microdialysis and silicon probe recordings in vivo. Journal of Neuroscience Methods 235, pp. 83-91. (10.1016/j.jneumeth.2014.06.031)
Crunelli, V., David, F., Leresche, N. and Lambert, R. C. 2014. Role for T-type Ca2+ channels in sleep waves. Pflugers Archiv European Journal of Physiology 466(4), pp. 735-745. (10.1007/s00424-014-1477-3)
Crunelli, V. and Di Giovanni, G. 2014. Monoamine modulation of tonic GABAA inhibition. Reviews in the Neurosciences 25(2), pp. 195-206. (10.1515/revneuro-2013-0059)

2013

David, F. et al. 2013. Essential thalamic contribution to slow waves of natural sleep. Journal of Neuroscience 33(50), article number: 19599. (10.1523/JNEUROSCI.3169-13.2013)
Connelly, W. M., Errington, A. C. and Crunelli, V. 2013. γ-Hydroxybutyric Acid (GHB) is not an agonist of extrasynaptic GABAA receptors. PLoS ONE 8(11), article number: e79062. (10.1371/journal.pone.0079062)
Connelly, W. M., Errington, A. C., Giovanni, G. D. and Crunelli, V. 2013. Metabotropic regulation of extrasynaptic GABAA receptors. Frontiers in Neural Circuits 7, article number: 171. (10.3389/fncir.2013.00171)
Yagüe, J. G., Cavaccini, A., Errington, A. C., Crunelli, V. and Di Giovanni, G. 2013. Dopaminergic modulation of tonic but not phasic GABAA-receptor-mediated current in the ventrobasal thalamus of Wistar and GAERS rats. Experimental Neurology 247, pp. 1-7. (10.1016/j.expneurol.2013.03.023)
Pirttimaki, T., Parri, H. R. and Crunelli, V. 2013. Astrocytic GABA transporter GAT-1 dysfunction in experimental absence seizures. The Journal of Physiology 591(4), pp. 823-833. (10.1113/jphysiol.2012.242016)
Lambert, R. C., Bessaïh, T., Crunelli, V. and Leresche, N. 2013. The many faces of T-type calcium channels. Pflugers Archiv European Journal of Physiology 466(3), pp. 415-423. (10.1007/s00424-013-1353-6)
Wade, J., McDaid, L., Harkin, J., Crunelli, V. and Kelso, S. 2013. Biophysically based computational models of astrocyte ~ neuron coupling and their functional significance. Frontiers in Computational Neuroscience 7, article number: 44. (10.3389/fncom.2013.00044)
Connelly, W. M., Fyson, S. J., Errington, A. C., McCafferty, C. P., Cope, D. W., Di Giovanni, G. and Crunelli, V. 2013. GABAB receptors regulate extrasynaptic GABAa receptors. Journal of Neuroscience 33(9), pp. 3780-3785. (10.1523/JNEUROSCI.4989-12.2013)
Crunelli, V. and Carmignoto, G. 2013. New vistas on astroglia in convulsive and non-convulsive epilepsy highlight novel astrocytic targets for treatment. The Journal of Physiology 591(4), pp. 775-785. (10.1113/jphysiol.2012.243378)
Hughes, S. and Crunelli, V. 2013. UP states rise from the depths. Nature Neuroscience 16(2), pp. 115-117. (10.1038/nn.3313)

2012

Wade, J., McDaid, L., Harkin, J. and Crunelli, V. 2012. Self-repair in a bidirectionally coupled astrocyte-neuron (AN) system based on retrograde signaling. Frontiers in Computational Neuroscience 6 (10.3389/fncom.2012.00076)
Leresche, N., Lambert, R. C., Errington, A. C. and Crunelli, V. 2012. From sleep spindles of natural sleep to spike and wave discharges of typical absence seizures: is the hypothesis still valid?. Pflügers Archiv European Journal of Physiology 463(1), pp. 201-212. (10.1007/s00424-011-1009-3)
Crunelli, V., Lőrincz, M. L., Errington, A. C. and Hughes, S. W. 2012. Activity of cortical and thalamic neurons during the slow (<1 Hz) rhythm in the mouse in vivo. Pflügers Archiv European Journal of Physiology 463(1), pp. 73-88. (10.1007/s00424-011-1011-9)
Errington, A. C., Hughes, S. W. and Crunelli, V. 2012. Rhythmic dendritic Ca2+ oscillations in thalamocortical neurons during slow non-REM sleep-related activity in vitro. The Journal of Physiology 590(16), pp. 3691-3700. (10.1113/jphysiol.2012.232132)

2011

Crunelli, V., Cope, D. W. and Terry, J. R. 2011. Transition to absence seizures and the role of GABAA receptors. Epilepsy Research 97(3), pp. 283-289. (10.1016/j.eplepsyres.2011.07.011)
Crunelli, V., Errington, A. C., Hughes, S. W. and Toth, T. 2011. The thalamic low-threshold Ca2+ potential: a key determinant of the local and global dynamics of the slow (<1 Hz) sleep oscillation in thalamocortical networks. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 369(1952), pp. 3820-3839. (10.1098/rsta.2011.0126)
Wade, J., McDaid, L. J., Harkin, J., Crunelli, V., Kelso, J. A. S. and Beiu, V. 2011. Exploring retrograde signaling via astrocytes as a mechanism for self repair. Presented at: Neural Networks (IJCNN), The 2011 International Joint Conference on, San Jose, CA, United States, July 31 - Aug 5 2011 Presented at Wade, J. J. et al. eds.The 2011 International Joint Conference on Neural Networks (IJCNN),. , (10.1109/IJCNN.2011.6033638)
Hughes, S. W., Lőrincz, M., Parri, H. R. and Crunelli, V. 2011. Infraslow (<0.1Hz) oscillations in thalamic relay nuclei: basic mechanisms and significance to health and disease states. Presented at: 26th International Summer School of Brain Research, Amsterdam, Netherlands, 29 June-2 July 2010 Presented at Van Someren, E. J. W. et al. eds.Slow Brain Oscillations of Sleep, Resting State and Vigilance: proceedings of the 26th International Summer School of Brain Research. Progress in brain research Vol. 193. Elsevier pp. 145-162., (10.1016/B978-0-444-53839-0.00010-7)
Errington, A. C., Di Giovanni, G., Crunelli, V. and Cope, D. W. 2011. mGluR control of interneuron output regulates feedforward tonic GABAA inhibition in the visual thalamus. Journal of Neuroscience 31(23), pp. 8669-8680. (10.1523/JNEUROSCI.0317-11.2011)
Errington, A. C., Gibson, K. M., Crunelli, V. and Cope, D. W. 2011. Aberrant GABAA receptor-mediated inhibition in cortico-thalamic networks of succinic semialdehyde dehydrogenase deficient mice. PLoS ONE 6(4), article number: e19021. (10.1371/journal.pone.0019021)
Wade, J. J., McDaid, L. J., Harkin, J., Crunelli, V. and Kelso, J. A. S. 2011. Bidirectional Coupling between Astrocytes and Neurons Mediates Learning and Dynamic Coordination in the Brain: A Multiple Modeling Approach. PLoS ONE 6(12), article number: e29445. (10.1371/journal.pone.0029445)
Errington, A. C., Cope, D. W. and Crunelli, V. 2011. Augmentation of Tonic GABAA Inhibition in Absence Epilepsy: Therapeutic Value of Inverse Agonists at Extrasynaptic GABAA Receptors. Advances in Pharmacological Sciences 2011, article number: 790590. (10.1155/2011/790590)

2010

Crunelli, V., Leresche, N. and Cope, D. W. 2010. GABAergic gain-of-function in absence seizures. Epilepsia 51(S5), pp. 23. (10.1111/j.1528-1167.2010.02809.x)
Errington, A. C., Renger, J., Uebele, V. and Crunelli, V. 2010. State-dependent firing determines intrinsic dendritic Ca2+ signaling in thalamocortical neurons. Journal of Neuroscience 30(44), pp. 14843-14853. (10.1523/JNEUROSCI.2968-10.2010)
Parri, H. R., Gould, T. M. and Crunelli, V. 2010. Sensory and cortical activation of distinct glial cell subtypes in the somatosensory thalamus of young rats. European Journal of Neuroscience 32(1), pp. 29-40. (10.1111/j.1460-9568.2010.07281.x)
Watson, J. et al. 2010. Gamma-hydroxybutyrate does not maintain self-administration but induces conditioned place preference when injected in the ventral tegmental area. International Journal of Neuropsychopharmacology 13(2), pp. 143-53. (10.1017/S1461145709990186)
Dreyfus, F. M. et al. 2010. Selective T-type calcium channel block in thalamic neurons reveals channel redundancy and physiological impact of I-Twindow. Journal of Neuroscience 30(1), pp. 99-109. (10.1523/JNEUROSCI.4305-09.2010)
Crunelli, V. and Hughes, S. W. 2010. The slow (< 1Hz) sleep rhythm: A dialogue of three cardinal oscillators. Nature Neuroscience 13(1), pp. 9-17. (10.1038/nn.2445)

2009

Cope, D. W. et al. 2009. Enhanced tonic GABA(A) inhibition in typical absence epilepsy. Nature Medicine 15(12), pp. 1392-1398. (10.1038/nm.2058)
Lorincz, M. L., Kékesi, K. A., Juhász, G., Crunelli, V. and Hughes, S. W. 2009. Temporal framing of thalamic relay-mode firing by phasic inhibition during the Alpha Rhythm. Neuron 63(5), pp. 683-696. (10.1016/j.neuron.2009.08.012)
Lorincz, M. L., Geall, F., Bao, Y., Crunelli, V. and Hughes, S. W. 2009. ATP-dependent infra-slow (<0.1 Hz) oscillations in thalamic networks. PLoS ONE 4(2), article number: e4447. (10.1371/journal.pone.0004447)
Chahboune, H. et al. 2009. DTI abnormalities in anterior corpus callosum of rats with spike-wave epilepsy. NeuroImage 47(2), pp. 459-466. (10.1016/j.neuroimage.2009.04.060)

2008

Lorincz, M. L. ., Crunelli, V. and Hughes, S. W. 2008. Cellular dynamics of cholinergically induces alpha (8-13 Hz) rhythms in sensory thalamic nuclei in vitro. Journal of Neuroscience 28(3), pp. 660-671. (10.1523/JNEUROSCI.4468-07.2008)
Blethyn, K. L., Hughes, S. W. and Crunelli, V. 2008. Evidence for electrical synapses between neurons of the nucleus reticularis thalami in the adult brain in vitro. Thalamus and Related Systems 4(1), pp. 13-20. (10.1017/S1472928807000325)
Destexhe, A. and Crunelli, V. 2008. Methods for computational neuroscience. Journal of Neuroscience Methods 169(2), pp. 269-70. (10.1016/j.jneumeth.2008.01.025)
Hughes, S. W. et al. 2008. Novel modes of rhythmic burst firing at cognitively-relevant frequencies in thalamocortical neurons. Brain Research 1235, pp. 12-20. (10.1016/j.brainres.2008.06.029)
Hughes, S. W., Lorincz, M., Cope, D. W. and Crunelli, V. 2008. NeuReal: an interactive simulation system for implementing artificial dendrites and large hybrid networks. Journal of Neuroscience Methods 169(2), pp. 290-301. (10.1016/j.jneumeth.2007.11.010)

2007

Tóth, T. I., Bessaïh, T., Leresche, N. and Crunelli, V. 2007. The properties of reticular thalamic neuron GABAA IPSCs of absence epilepsy rats lead to enhanced network excitability. European Journal of Neuroscience 26(7), pp. 1832-1844. (10.1111/j.1460-9568.2007.05800.x)
Crunelli, V. and Gerhardt, G. 2007. Editorial. Journal of Neuroscience Methods 163(2), pp. 189. (10.1016/j.jneumeth.2007.05.015)
Destexhe, A., Hughes, S. W., Rudolph, M. and Crunelli, V. 2007. Are corticothalamic ‘up’ states fragments of wakefulness?. Trends in Neurosciences 30(7), pp. 334-342. (10.1016/j.tins.2007.04.006)
Hughes, S. W. and Crunelli, V. 2007. Just a phase they're going through: The complex interaction of intrinsic high-threshold bursting and gap junctions in the generation of thalamic α and θ rhythms. International Journal of Psychophysiology 64(1), pp. 3-17. (10.1016/j.ijpsycho.2006.08.004)
Parri, R. and Crunelli, V. 2007. Astrocytes target presynaptic NMDA receptors to give synapses a boost. Nature Neuroscience 10(3), pp. 271-273. (10.1038/nn0307-271)

2006

Crunelli, V. 2006. Pathophysiological mechanisms of absence seizures. Epilepsia 47(Supp 3), pp. 262-262. (10.1111/j.1528-1167.2006.00715_80.x)
Zhu, L., Blethyn, K. L., Cope, D. W., Tsomaia, V., Crunelli, V. and Hughes, S. W. 2006. Nucleus- and species-specific properties of the slow (<1 hz) sleep oscillation in thalamocortical neurons. Neuroscience 141(2), pp. 621-36. (10.1016/j.neuroscience.2006.04.069)
Blethyn, K. L., Hughes, S. W., Tóth, T. I., Cope, D. W. and Crunelli, V. 2006. Neuronal basis of the slow (<1 Hz) oscillation in neurons of the nucleus reticularis thalami in vitro. Journal of Neuroscience 26(9), pp. 2474-2486. (10.1523/JNEUROSCI.3607-05.2006)
Bessaih, T. et al. 2006. Nucleus-specific abnormalities of GABAergic synaptic transmission in a genetic model of absence seizures. Journal of Neurophysiology 96(6), pp. 3074-3081. (10.1152/jn.00682.2006)
Crunelli, V., Cope, D. W. and Hughes, S. W. 2006. Thalamic T-type Ca2+ channels and NREM sleep. Cell Calcium 40(2), pp. 175-190. (10.1016/j.ceca.2006.04.022)
Crunelli, V., Emri, Z. and Leresche, N. 2006. Unravelling the brain targets of γ-hydroxybutyric acid. Current Opinion in Pharmacology 6(1), pp. 44-52. (10.1016/j.coph.2005.10.001)

2005

Cope, D. W., Hughes, S. W. and Crunelli, V. 2005. GABAA Receptor-Mediated Tonic Inhibition in Thalamic Neurons. Journal of Neuroscience 25(50), pp. 11553-11563. (10.1523/JNEUROSCI.3362-05.2005)
Hughes, S. W. and Crunelli, V. 2005. Thalamic mechanisms of EEG alpha rhythms and their pathological implications. The Neuroscientist 11(4), pp. 357-372. (10.1177/1073858405277450)
Holter, J., Davies, J., Leresche, N., Crunelli, V. and Carter, D. 2005. Identification of two further splice variants of GABABR1 characterizes the conserved micro-exon 4 as a hot spot for regulated splicing in the rat brain. Journal of Molecular Neuroscience 26(1), pp. 99-108. (10.1385/JMN:26:1:099)
Crunelli, V., Toth, T. I., Cope, D., Blethyn, K. and Hughes, S. W. 2005. The "window' T-type calcium current in brain dynamics of different behavioural states. The Journal of Physiology 562(1), pp. 121-129. (10.1113/jphysiol.2004.076273)
Holter, J., Carter, D., Leresche, N., Crunelli, V. and Vincent, P. 2005. A TASK3 channel (KCNK9) mutation in a genetic model of absence epilepsy. Journal of Molecular Neuroscience 25(1), pp. 37-51. (10.1385/JMN:25:1:037)

2004

Hughes, S. W. et al. 2004. Synchronized oscillations at alpha and theta frequencies in the lateral geniculate nucleus. Neuron, pp. 253-268. (10.1016/S0896-6273(04)00191-6)
Manning, J. P. A., Richards, D. A., Leresche, N., Crunelli, V. and Bowery, N. G. 2004. Cortical-area specific block of genetically determined absence seizures by ethosuximide. Neuroscience 123(1), pp. 5-9. (10.1016/j.neuroscience.2003.09.026)

2003

Gervasi, N., Monnier, Z., Vincent, P., Paupardin-Tritsch, D., Hughes, S. W., Crunelli, V. and Leresche, N. 2003. Pathway-specific action of gamma-hydroxybutyric acid in sensory thalamus and its relevance to absence seizures. Journal of Neuroscience 23(36), pp. 11469-11478.
Richards, D. A. et al. 2003. Targeting thalamic nuclei is not sufficient for the full anti-absence action of ethosuximide in a rat model of absence epilepsy. Epilepsy Research 54(2-3), pp. 97-107. (10.1016/S0920-1211(03)00060-3)
Emri, Z., Antal, K. and Crunelli, V. 2003. The impact of corticothalamic feedback on the output dynamics of a thalamocortical neurone model: The role of synapse location and metabotropic glutamate receptors. Neuroscience 117(1), pp. 229-239. (10.1016/S0306-4522(02)00759-5)
Parri, R. and Crunelli, V. 2003. An astrocyte bridge from synapse to blood flow. Nature Neuroscience 6(1), pp. 5-6. (10.1038/nn0103-5)
Parri, H. R. and Crunelli, V. 2003. The role of Ca2+ in the generation of spontaneous astrocytic Ca2+ oscillations. Neuroscience 120(4), pp. 979-992. (10.1016/S0306-4522(03)00379-8)

2002

Crunelli, V. et al. 2002. Novel neuronal and astrocytic mechanisms in thalamocortical loop dynamics. Philosophical Transactions of the Royal Society B: Biological Sciences 357(1428), pp. 1675-1693. (10.1098/rstb.2002.1155)
Emri, Z., Antal, K. and Crunelli, V. 2002. Regulation of thalamic output by the metabotropic glutamate receptor-mediated component of the corticothalamic EPSP; a simulation study. The Journal of Physiology 544, pp. 79p-79p.
Parri, R. and Crunelli, V. 2002. Mechanism of spontaneous calcium oscillations in ventrobasal thalamus astrocytes. The Journal of Physiology 544, article number: SO55.
Toth, T. I. and Crunelli, V. 2002. Large-scale neuronal network models based on a probabilistic connectivity principle. Proceedings of the Physiological Society: Cellular & Integrative Neuroscience Abstracts, article number: 544P, S07.
Toth, T. I. and Crunelli, V. 2002. Modelling large scale neuronal networks using 'average neurones'. Neuroreport 13(14), pp. 1785-1788.
Crunelli, V. and Leresche, N. 2002. Childhood absence epilepsy: genes, channels, neurons and networks. Nature Reviews Neuroscience 3(5), pp. 371-382. (10.1038/nrn811)
Parri, R. and Crunelli, V. 2002. Astrocytes, spontaneity, and the developing thalamus. Journal of Physiology - Paris: An International Review Journal for the Neurosciences 96(3-4), pp. 221-230. (10.1016/S0928-4257(02)00009-8)
Crunelli, V., Charpier, S., Leresche, N. and Slaght, S. 2002. Activity of thalamic reticular neurons during spontaneous genetically determined spike and wave discharges. Journal of Neuroscience, pp. 2323-2334.
Hughes, S. W., Cope, D. W., Blethyn, K. L. and Crunelli, V. 2002. Cellular Mechanisms of the Slow (<1 Hz) Oscillation in Thalamocortical Neurons In Vitro.. Neuron, pp. 947-958. (10.1016/S0896-6273(02)00623-2)
Hughes, S. W., Blethyn, K. L., Cope, D. W. and Crunelli, V. 2002. Properties and origin of spikelets in thalamocortical neurones in vitro. Neuroscience 110(3), pp. 395-401. (10.1016/S0306-4522(01)00577-2)

2001

Parri, H. R. and Crunelli, V. 2001. Pacemaker calcium oscillations in thalamic astrocytes in situ. NeuroReport 12(18), pp. 3897-3900. (10.1097/00001756-200112210-00008)
Holter, J. L., Humphries, A., Crunelli, V. and Carter, D. 2001. Optimisation of methods for selecting candidate genes from cDNA array screens: application to rat brain punches and pineal. Journal of Neuroscience Methods 112(2), pp. 173-184. (10.1016/S0165-0270(01)00466-6)
Toth, T. I. and Crunelli, V. 2001. Estimation of the activation and kinetic properties of I-Na and I-K from the time course of the action potential. Journal of Neuroscience Methods 111(2), pp. 111-126. (10.1016/S0165-0270(01)00433-2)
Crunelli, V., Gould, T. M. and Parri, H. R. 2001. Spontaneous astrocytic Ca2+ oscillations in situ drive NMDAR-mediated neuronal excitation. Nature Neuroscience, pp. 803-812. (10.1038/90507)

Mae fy ymchwil yn canolbwyntio ar y mecanweithiau cellog a rhwydwaith sy'n gweithredu yn y thalamws a'r cortex yn ystod epilepsi cwsg ac absenoldeb.

Yn ddiweddar, fe wnaethom hefyd ddatblygu diddordeb yn y mecanweithiau sy'n ymwneud â signalau astrocyte-niwron.

Mae fy ngrŵp amlddisgyblaethol yn defnyddio cyfuniad o dechnegau delweddu electroffisiolegol, morffolegol, imiwnocemegol a chonfocal mewn modelau arferol a thrawsgenig mewn vivo ac mewn modelau anifeiliaid in vitro, yn ogystal â dull cyfrifiadurol ar gyfer efelychu gweithgareddau rhwydwaith niwronol ac astrocytig.

Mecanweithiau cysgu

Un o'r gweithgareddau trydanol mwyaf sylfaenol sy'n digwydd mewn niwronau thalamig a chortigol yn ystod cwsg naturiol yw'r 'osgiliad cwsg araf'. Mae'r panel uchaf yn Ffigur 1 isod yn dangos enghraifft nodweddiadol o'r gweithgaredd hwn a gofnodwyd o niwron thalamig.

Mae'r 'osgiliad cwsg araf' yn cael ei nodweddu gan bresenoldeb dau botensial bilen (a elwir yn wladwriaethau UP a DOWN), sy'n cael eu cynhyrchu trwy newid 'ymlaen' ac 'i ffwrdd' cydrannau ffenestr y foltedd isel a weithredir, T-math Ca²⁺ cyfredol (I T). Yn y panel gwaelod o Ffigur 1, mae'r ddau gerrynt niwronal eraill sy'n chwarae rhan hanfodol yn yr osgiliadau araf yn cael eu darlunio, hy Ydw, wedi'i actifadu gan hyperpolarization Na + / K + cyfredol, ac I CAN, Ca^{2 +}-activated cerrynt cation detholus. Manylion pellach yn ein cyhoeddiadau: Hughes et al., Niwron 33 (2002) 947-958; Blethyn et al., Journal of Neuroscience 26 (2006) 2474-2486; Crunelli et al., Calsiwm Cell 40 (2006) 175-190; a Destexhe et al., Tueddiadau mewn Niwrowyddoniaeth 30 (2007) 334-342.

Mecanweithiau pathoffisiolegol Epilepsi Absenoldeb

Mae epilepsi absenoldeb yn fath anorchfygol o epilepsi sy'n effeithio ar blant a phobl ifanc yn bennaf. Mae pob trawiad yn cynnwys nam sydyn a byr ar ymwybyddiaeth, sy'n cyd-fynd â syllu gwag, diffyg ymateb i symbyliadau allanol, ac ymddangosiad gweithgaredd patholegol nodweddiadol, a elwir yn 'ryddhad spike-and-wave' (dangosir yn Ffigur 2A, dde).

Mae'r 'rhyddhau sbectol' yn cael ei gynhyrchu gan weithgareddau trydanol annormal niwronau cortigol a thalamig (a ddangosir yn Ffigur 2B-D, mae cod lliw yn cyfeirio at y gwahanol fathau o gelloedd a ddangosir yn y diagram sgematig). Sylwch ar danio'r niwronau GABAergic cryf iawn yn y niwclews reticularis thalami (NRT) (gweler y ffilm isod) a thawelwch trydanol y niwronau thalamocortical (TC).

Gan mai niwronau TC yw'r rhai sy'n trosglwyddo symbyliadau synhwyraidd, mae eu hyperpolareiddio a'u diffyg tanio yn egluro pam nad yw plant yn ymatebol yn ystod trawiad ar absenoldeb.

Manylion pellach yn ein cyhoeddiadau: Crunelli a Leresche, Nature Reviews Neuroscience, 3 (2002) 371-382; Slaght et al. Journal of Neuroscience 22 (2002) 2323-2334; a Manning et al., Niwrowyddoniaeth 123 (2004) 5-9.

signalau astrocyte-niwron

Nid yw astrocytes (math o gelloedd glial) bellach yn cael eu hystyried fel darparu cefnogaeth fecanyddol yn unig i niwronau ac yn rheoli crynodiad allgellog ïonau a metabolion allweddol, ond dangoswyd eu bod yn cyfrannu'n weithredol at drosglwyddo gwybodaeth niwronol ar y lefel synaptig.

Gan fod astrocytes yn gelloedd na ellir eu cyffroi mae eu 'actifadu' yn cynnwys codiadau dros dro mewn Ca²⁺ mewngellog sydd wedyn yn arwain at ryddhau trosglwyddyddion yn fetrigl, gan gynnwys glutamad ac ATP.

Mae'r sylweddau hyn a ryddhawyd yn seryddol wedyn yn gweithredu ar niwronau (gan actifadu derbynyddion NMDA yn ddelfrydol) rhyddhau trosglwyddydd modiwleiddio neu reoli effeithiolrwydd synaptig. Yn wir, gall astrocytes hyd yn oed gynhyrchu tonnau Ca^{2 +} digymell a lleol, yn absenoldeb unrhyw weithgaredd niwronau.

Manylion pellach yn ein cyhoeddiadau: Parri et al., Nature Neuroscience 4 (2001) 803-812; Parri et al., Niwrowyddoniaeth 120 (2003) 979-992; a Parri a Crunelli Natur Niwrowyddoniaeth 10 (2007) 271-273

Cyfleusterau grŵp

Mae gan fy labordy chwe gorsaf electroffisiolegol (tair ar gyfer recordiadau patsh- a thri ar gyfer recordiadau miniog-electrod), un microsgop confocal (Odyssey, Thermo Noran, UDA) gyda chyfleusterau recordio patch-electrod in vitro , un microsgop sganio laser 2-photon (Ultima, Prairie Technology, UDA) gyda chyfleusterau recordio vivo ac in vitro patch- a sharp-electrode, cyfres ar gyfer in vivo Recordiadau trydanol a chymhwyso cyffuriau lleol mewn modelau sy'n symud yn rhydd, un clwstwr o nodau prosesydd deuol 11 ar gyfer efelychiadau cyfrifiadurol, meddalwedd a ddatblygwyd yn fewnol ar gyfer arbrofion clamp deinamig, a chyfleusterau ar gyfer dadansoddiad morffolegol ac imiwnocemegol ôl-hoc o niwronau a astrocytes.

Grantiau cyfredol

Mecanweithiau thalamig cellog o dan amodau ffisiolegol a phatholegol

Ffynhonnell: Wellcome Trust
Hyd: 5 mlynedd (o Hydref 2003) (Grant Rhaglen)
Swm: £1,246,652

Cyffyrdd bwlch thalamig niwronau: hunaniaeth, lleoliad a rôl mewn rhythmau EEG araf o wladwriaethau ffisiolegol (patho)

Ffynhonnell: Wellcome Trust
Duration: 3 years (from January 2006)
Swm: £239,867

Ymchwiliad moleciwlaidd a chellol rhyngweithiadau niwro-astroglia: Deall swyddogaeth yr ymennydd a chamweithrediad

Ffynhonnell: Yr Undeb Ewropeaidd (FP7)
Hyd: 4 blynedd (o fis Ionawr 2008) (gyda 5 partner)
Swm: £2,104,762

Yn Ysgol y Biowyddorau ym Mhrifysgol Caerdydd, rwy'n addysgu niwrowyddoniaeth a niwroffisioleg i fyfyrwyr gwyddoniaeth israddedig (modiwlau Bi2432, BI3451, Bi3452) ac i fyfyrwyr deintyddol. Yn y Gyfadran Meddygaeth a Llawfeddygaeth ym Mhrifysgol Malta, rwy'n addysgu niwrowyddoniaeth a chlefydau niwrolegol i fyfyrwyr meddygol.

Rwyf hefyd yn goruchwylio myfyrwyr Meistr a PhD yn y ddwy Brifysgol.

Laurea (Cemeg Pur), Prifysgol Catania, Catania, Yr Eidal (1974)
Cydymaith Ymchwil Ôl-ddoethurol, Labordy Niwroffarmacoleg, Sefydliad Mario Negri ar gyfer Ymchwil Ffarmacolegol, Milan, yr Eidal (1976-1978)
Cydymaith Ymchwil Ôl-ddoethurol, Cyngor Ymchwil Meddygol, Uned Ffarmacoleg Niwrocemegol, Caergrawnt, y DU (1979)
Cydymaith Ymchwil Ôl-ddoethurol, Canolfan Niwrowyddoniaeth, Sefydliad Gwyddoniaeth Weizmann, Rehovot, Israel (1980)

Anrhydeddau a dyfarniadau

Cymrodor, Academi Gwyddorau Meddygol (Etholwyd (2010)
Cymrawd Academia Europaea (etholwyd 2010)
Cymrodyr, Siambr Gwyddonwyr Malta (etholwyd 2012)
Cymrodor, Cymdeithas Ddysgedig Cymru (etholwyd 2012)

Safleoedd academaidd blaenorol

Cymrawd Ymchwil Ymddiriedolaeth Wellcome, Adran Ffarmacoleg, Ysgol Feddygol Ysbyty San Siôr, Llundain, DU (1981-1984)
Darlithydd Ymddiriedolaeth Wellcome, Adran Ffarmacoleg, Ysgol Feddygol Ysbyty San Siôr, Llundain, DU (1985)
Darlithydd Ffarmacoleg, Adran Ffarmacoleg, Ysgol Feddygol Ysbyty San Siôr, Llundain, y DU (1986-1988)
Uwch Ddarlithydd Ffarmacoleg, Adran Ffarmacoleg, Ysgol Feddygol Ysbyty San Siôr, Llundain, y DU (1989)
Uwch Ddarlithydd mewn Gwyddoniaeth Weledol, Adran Gwyddor Gweledol, Sefydliad Offthalmoleg, Llundain, y DU (1989-1991)
Athro Niwrowyddoniaeth, Ysgol y Biowyddorau, Prifysgol Caerdydd, Caerdydd, y DU (1991-presennol)
Pennaeth, Adran Ffisioleg, Prifysgol Caerdydd, Caerdydd, y DU (1994-1998)
Athro ymweliadol, Université Pierre et Marie Curie, 9 quai Saint-Bernard, Paris, Ffrainc (1998-2002)
Pennaeth, Is-adran Niwrowyddoniaeth, Ysgol y Biowyddorau, Prifysgol Caerdydd, Caerdydd, y DU (2011-2015)
Athro Cyswllt, Adran Ffisioleg a Biocemeg, Cyfadran Meddygaeth a Llawfeddygaeth, Prifysgol Malta, Msida, Malta (2016-hyd yn hyn)

Pwyllgorau ac adolygu

Editor-in-Chief Journal of Neuroscience Methods (1999-2017)
Golygydd, British Journal of Pharmacology (1991-1996)
Golygydd, Niwron Glia Bioleg (2001-2013)
Golygydd, Thalamus and Related Systems (2003-2009)
Golygydd, Journal of Neural Systems (2004-2008)
Golygydd Cynghori, Xjenza Ar-lein (2012-i'r presennol)

Array

Contact Details

Crunelli@caerdydd.ac.uk
+44 29208 74091

Deall epilepsi pellter meddwl

10 June 2018