Dr Julie Albon

Lecturer, Optic Nerve Head Group Leader, Person designate, Cathays Park HTA satellite licence

: Ar gael fel goruchwyliwr ôl-raddedig

Research Overview

My research focuses on investigating the human optic nerve head, in particular the lamina cribrosa and its insertion region into the sclera, with an overall aim to i) understand how ONH structure and connective tissue micro- and nano-organisation influences a) its biomechanics and b) its susceptibility to the development of glaucomatous optic neuropathy. In the Optic Nerve Head Group, we use a number in ex vivo and in vivo techniques to analyse the cells and connective tissue (collagen, elastin and proteoglycans) and overall architecture of the 3D optic nerve head (non-linear microscopy, xray microtomography, OCT) alters in ageing and glaucoma. In addition, we are investigating the regional micro- and nano-organisation of connective tissue fibres, collagen and elastin (SAXS, SALS, second harmonic imaging, two photon excited fluorescence) in the ONH, and how they influence the biomechanical behaviour of the human ONH.

Teaching Overview

$acirc; Module leader for OP1205 Cells to System for first year undergraduates in the BSc Optometry and Vision Sciences
$acirc; Lecturer for OP1205 (lectures, laboratory practical sessions and tutorials)
$acirc; Lecturer in Working safely in Cardiff University course to OPTOM postgraduates
$acirc; Lecturer in COSHH course to OPTOM postgraduates
$acirc; Lecturer in postgraduate MSc Clinical Optometry.
$acirc; Supervisor of third year BSc research project students
$acirc; Supervisor of Erasmus postgraduate students$acirc; MSc/research projects for University of Riga, Latvia.

Date
Type

2023

Ahmad, F., Soe, S., Albon, J., Errington, R. and Theobald, P. 2023. Quantifying the microstructural and biomechanical changes in the porcine ventricles during growth and remodelling. Acta Biomaterialia 171, pp. 166-192. (10.1016/j.actbio.2023.09.044)

2022

Bartlett, R. L. et al. 2022. Quantifying biomarkers of axonal degeneration in early glaucoma to find the disc at risk. Scientific Reports 12(1), article number: 9366. (10.1038/s41598-022-12036-4)
Rumney, B. M. et al. 2022. Characterisation of carapace composition in developing and adult ostracods (Skogsbergia lerneri) and its potential for biomaterials. Marine Biology 169(6), article number: 78. (10.1007/s00227-022-04047-6)
Kulmaganbetov, M. et al. 2022. Textural feature analysis of optical coherence tomography phantoms. Electronics 11(4), article number: 669. (10.3390/electronics11040669)
Rumney, B. M. et al. 2022. The ultrastructural development and 3D reconstruction of the transparent carapace of the ostracod Skogsbergia lerneri. Marine Biology 169, article number: 35. (10.1007/s00227-021-04006-7)

2021

Parker, A. R., Palka, B. P., Albon, J., Meek, K. M., Holden, S. and Malik, F. T. 2021. Biomimetic transparent eye protection inspired by the carapace of an ostracod (crustacea). Nanomaterials 11(3), article number: 663. (10.3390/nano11030663)

2020

Morgan, S. R. et al. 2020. Establishment of long-term ostracod epidermal culture. In Vitro Cellular and Developmental Biology - Animal 56(9), pp. 760-772. (10.1007/s11626-020-00508-8)
Huang, C., Albon, J., V. Ljubimov, A. and Grant, M. B. 2020. Stem cells in the eye. In: Principles of Tissue Engineering Fifth Edition. Elsevier Inc., pp. 1115-1133., (10.1016/B978-0-12-818422-6.00062-9)

2018

Nosch, D. S., Pult, H., Albon, J., Purslow, C. and Murphy, P. J. 2018. Does air gas aesthesiometry generate a true mechanical stimulus for corneal sensitivity measurement?. Clinical and Experimental Optometry 101(2), pp. 193-199. (10.1111/cxo.12603)

2017

Feola, A. J. et al. 2017. Deformation of the lamina cribrosa and optic nerve due to changes in cerebrospinal fluid pressure. Investigative Ophthalmology & Visual Science 58(4), pp. 2070-2078. (10.1167/iovs.16-21393)

2016

Coudrillier, B. et al. 2016. Effects of peripapillary scleral stiffening on the deformation of the lamina cribrosa. Investigative Ophthalmology & Visual Science 57(6), pp. 2666-2677. (10.1167/iovs.15-18193)
Nosch, D. S., Pult, H., Albon, J., Purslow, C. and Murphy, P. J. 2016. Relationship between corneal sensation, blinking, and tear film quality. Optometry and Vision Science 93(5), pp. 471-481. (10.1097/OPX.0000000000000827)
Coudrillier, B. et al. 2016. Phase-contrast micro-computed tomography measurements of the intraocular pressure-induced deformation of the porcine lamina cribrosa. IEEE Transactions on Medical Imaging 35(4), pp. 988-999. (10.1109/TMI.2015.2504440)

2015

Gardner, S. J., White, N., Albon, J., Knupp, C., Kamma-Lorger, C. S. and Meek, K. M. 2015. Measuring the refractive index of bovine corneal stromal cells using quantitative phase imaging. Biophysical Journal 109(8), pp. 1592-1599. (10.1016/j.bpj.2015.08.046)
Jones, H. J., Girard, M. J., White, N., Fautsch, M. P., Morgan, J. E., Ethier, C. R. and Albon, J. 2015. Quantitative analysis of three-dimensional fibrillar collagen microstructure within the normal, aged and glaucomatous human optic nerve head. Journal of the Royal Society Interface 12(106), article number: 20150066. (10.1098/rsif.2015.0066)
Zhang, L., Albon, J., Jones, H., Gouget, C. L. M., Ethier, C. R., Goh, J. C. H. and Girard, M. J. A. 2015. Collagen microstructural factors influencing optic nerve head biomechanics. Investigative Ophthalmology & Visual Science 56(3), pp. 2031-2042. (10.1167/iovs.14-15734)

2014

Anantrasirichai, N. et al. 2014. Adaptive-weighted bilateral filtering and other pre-processing techniques for optical coherence tomography. Computerized Medical Imaging and Graphics 38(6), pp. 526-539. (10.1016/j.compmedimag.2014.06.012)

2013

North, R. V. et al. 2013. Lamina cribrosa pore analysis in glaucomatous human optic nerve heads in vivo. Presented at: Association for Research in Vision and Ophthalmology, Seattle, WA, USA, 5-9 May 2013.

2012

Mortlock, K. E. et al. 2012. Three dimensional (3D) segmentation of normal and glaucomatous optic nerve heads in 1050nm optical coherence tomography (OCT) datasets. Presented at: Association for Research in Vision and Ophthalmology, Fort Lauderdale, FL., USA, 6-9 May 2012.

2011

Girard, M. J. A. et al. 2011. Quantitative mapping of scleral fiber orientation in normal rat eyes. Investigative Ophthalmology and Visual Science 52(13), pp. 9684-9693. (10.1167/iovs.11-7894)
Taylor, S., Calder, C., Albon, J., Erichsen, J. T., Boulton, M. E. and Morgan, J. E. 2011. Involvement of the CD200 receptor complex in microglia activation in experimental glaucoma. Experimental Eye Research 92(5), pp. 338-343. (10.1016/j.exer.2011.01.012)
Mi, S., Dooley, E. P., Albon, J., Boulton, M. E., Meek, K. M. A. and Kamma-Lorger, C. S. 2011. Adhesion of laser in situ keratomileusis-like flaps in the cornea: effects of crosslinking, stromal fibroblasts, and cytokine treatment. Journal of Cataract & Refractive Surgery 37(1), pp. 166-172. (10.1016/j.jcrs.2010.07.028)
Ma, A., Zhao, B., Boulton, M. and Albon, J. 2011. A role for Notch signaling in corneal wound healing. Wound Repair and Regeneration 19(1), pp. 98-106. (10.1111/j.1524-475X.2010.00648.x)

2010

Kisiswa, L., Albon, J., Morgan, J. E. and Wride, M. A. 2010. Cellular inhibitor of apoptosis (cIAP1) is down-regulated during Retinal ganglion cell (RGC) maturation. Experimental Eye Research 91(5), pp. 739-747. (10.1016/j.exer.2010.08.024)
Mortlock, K. E. et al. 2010. 1050nm optical coherence tomography of the ageing and glaucomatous optic nerve head. Presented at: EUROMECH Colloquim, Imperial College, London, UK, 26-28 July, 2010.
Kisiswa, L., Dervan, A. G., Albon, J., Morgan, J. E. and Wride, M. A. 2010. Retinal ganglion cell death postponed: giving apoptosis a break?. Ophthalmic Research 43(2), pp. 61-78. (10.1159/000247591)

2009

Kamma-Lorger, C. S., Boote, C., Hayes, S., Albon, J., Boulton, M. E. and Meek, K. M. A. 2009. Collagen ultrastructural changes during stromal wound healing in organ cultured bovine corneas. Experimental Eye Research 88(5), pp. 953-959. (10.1016/j.exer.2008.12.005)

2007

Ma, A., Boulton, M. E., Zhao, B., Connon, C. J., Cai, J. and Albon, J. 2007. A role for notch signaling in human corneal epithelial cell differentiation and proliferation. Investigative Ophthalmology & Visual Science 48(8), pp. 3576-3585. (10.1167/iovs.06-1373)
Albon, J. et al. 2007. Connective tissue structure of the tree shrew optic nerve and associated ageing changes. Investigative Ophthalmology and Visual Science 48(5), pp. 2134-2144. (10.1167/iovs.06-0084)

2006

Jarrett, S. G., Albon, J. and Boulton, M. E. 2006. The contribution of DNA repair and antioxidants in determining cell type-specific resistance to oxidative stress. Free Radical Research 40(11), pp. 1155-1165. (10.1080/10715760600876613)
Carrington, L. M., Albon, J., Anderson, I., Kamma-Lorger, C. S. and Boulton, M. E. 2006. Differential regulation of key stages in early corneal wound healing by TGF-beta isoforms and their inhibitors. Investigative Ophthalmology and Visual Science 47(5), pp. 1886-1894. (10.1167/iovs.05-0635)
Morgan, J. E., Datta, A. V., Erichsen, J. T., Albon, J. and Boulton, M. E. 2006. Retinal ganglion cell remodelling in experimental glaucoma. In: Hollyfield, J. G., Anderson, R. E. and LaVail, M. M. eds. Retinal Degenerative Diseases. Advances in Experimental Medicine and Biology Vol. 572. New York: Springer, pp. 397-402.

2004

Boulton, M. and Albon, J. 2004. Stem cells in the eye. International Journal of Biochemistry and Cell Biology 36(4), pp. 643-657. (10.1016/j.biocel.2003.10.013)

2003

Jones, A. L., Carrington, L. M., Albon, J., Rosen, E. and Boulton, M. E. 2003. Localisation of receptors to estradiol, progesterone and androgen in the bovine cornea. Investigative Ophthalmology & Visual Science 44(E-Abst)
Albon, J., Ma, A., Cai, J. and Boulton, M. E. 2003. Localisation of notch and delta in the human cornea [Abstract]. Investigative Ophthalmology & Visual Science 44(E-Abst)

2000

Albon, J., Tullo, A. B., Aktar, S. and Boulton, M. E. 2000. Apoptosis in the endothelium of human corneas for transplantation. Investigative Ophthalmology and Visual Science 41(10), pp. 2887-2893.
Albon, J., Karwatowski, W. S., Easty, D. L., Sims, T. J. and Duance, V. C. 2000. Age related changes in the non-collagenous components of the extracellular matrix of the human lamina cribrosa. British Journal of Ophthalmology 84(3), pp. 311-317. (10.1136/bjo.84.3.311)
Albon, J., Purslow, P. P., Karwatowski, W. S. and Easty, D. L. 2000. Age related compliance of the lamina cribrosa in human eyes. British Journal of Ophthalmology 84(3), pp. 318-323. (10.1136/bjo.84.3.318)

1999

Davies, Y., Lewis, D., Fullwood, N. J., Nieduszynski, I. A., Marcyniuk, B., Albon, J. and Tullo, A. 1999. Proteoglycans on normal and migrating human corneal endothelium. Experimental Eye Research 68(3), pp. 303-311. (10.1006/exer.1998.0609)

1997

Albon, J., Tullo, A. B. and Boulton, M. E. 1997. Apoptosis in the endothelium of organ-cultured human corneas. Investigative Ophthalmology & Visual Science 38(4), pp. 3374-3374.

1995

Karwatowski, W., Jeffries, T., Duance, V., Albon, J., Bailey, A. and Easty, D. 1995. Preparation of Bruch's membrane and analysis of the age-related changes in the structural collagens. British Journal of Ophthalmology 79(10), pp. 944-952. (10.1136/bjo.79.10.944)
Jeffery, G., Evans, A., Albon, J., Duance, V., Neal, J. and Dawidek, G. 1995. The human optic nerve: fascicular organisation and connective tissue types along the extra-fascicular matrix. Anatomy and Embryology 191(6), pp. 491-502.
Albon, J., Karwatowski, W., Avery, N., Easty, D. and Duance, V. 1995. Changes in the collagenous matrix of the aging human lamina cribrosa. British Journal of Ophthalmology 79(4), pp. 368-375.

1994

Albon, J., Purslow, P. P., Duance, V. C., Easty, D. L. and Karwatowski, W. 1994. Mechanical compliance of the aging human lamina-cribrosa [Abstract]. Investigative Ophthalmology & Visual Science 35(4), pp. 1645-1645.

Research Topics and Related Projects:

Throughout my research history I have studied the ageing of ocular tissues using a range of cell biology, biochemical and microscopy techniques. My research to date has focussed on the two opposites ends of the eye: the optic nerve head and the cornea! My PhD, at Bristol University, investigated how the different components (collagen, elastin, proteoglycans) of the human lamina cribrosa extracellular matrix altered with age, and their effect on its biomechanical behaviour. Following my time as a senior clinical scientist working in the Manchester Healthcare NHS Trust Corneal Transplant Service Eye Bank, an interest in the cornea developed. This led to research into the cells and extracellular matrix in corneal wound healing, the potential of organ culture stored corneal donor material for use in epithelial stem cell transplants, and factors involved in epithelial stem cell homeostasis (including Notch signalling!). This works continues to date.

My current research continues into the human optic nerve head, in particular the lamina cribrosa and its insertion region into the sclera, with an overall aim to understand how ONH structure and connective tissue micro- and nano-organisation influences a) its biomechanics and b) its susceptibility to the development of glaucomatous optic neuropathy. In the Optic Nerve Head Research Group, we use a number in ex vivo and in vivo techniques to analyse the cells and connective tissue (collagen, elastin and proteoglycans), and overall architecture of the 3D optic nerve head (non-linear microscopy, xray microtomography, OCT) in ageing and glaucoma. In addition, we are investigating the regional micro- and nano-organisation of connective tissue fibres, collagen and elastin (SAXS, SALS, second harmonic imaging, two photon excited fluorescence) in the ONH, and how they influence the biomechanical behaviour of the human ONH.

Microperimetry results in a healthy eye.

Figure 1. 3D reconstruction of a human optic nerve head following non-linear microscopy. Left: Fibrillar collagen (SHG: green) and elastin (TPEF:red) in the LC and central retinal vessels in 2D.

Figure 2. Non-linear microscopy in human LC: fibrillar collagen (SHG: green) and elastin (TPEF:red)

We use optical coherence tomography techniques (1050nm, enhanced depth imaging, commercial) to create 3D volumetric datasets of the normal and glaucomatous optic nerve head in vivo in order to characterise ONH parameters, especially those in the lamina cribrosa, that have the potential to stage and/or predict glaucomatous optic neuropathy in ONHs at risk. We are examining the chronicity/relationship of events that take place in the ONH in comparison to those in the retina.

3D rendered human optic nerve head OCT image

The overall aim of my research is to identify changes in the ONH and/or retinal structure that can be used to predict and/or stage the development of open angle glaucoma i.e. to evaluate biomarkers as indicators of disease. This is in conjunction with a long term aim to develop therapies that can be applied at critical times before onset or at early onset of disease in order to prevent retinal ganglion cell axon degeneration and vision loss.

This research is underpinned by a multidisciplinary team of investigators within the College of Life Sciences and Biomedical Sciences (Professors Rachel North, James Morgan, Bruce Caterson, Vic Duance, Keith Meek, Phil Stephens, Dr Craig Boote). Nick White and James Fergusson of the Vision Science Bioimaging Laboratories (VSBL) are instrumental in this work, as are our external collaborators (see below).

Funding:

Albon J, North RV, Morgan JE. £52,688, The use of 1050nm OCT to identify changes of optic nerve head pathophysiology in glaucoma, College of Optometrists, 2011-2014.

Albon J, North RV. £1400, Retinal layer analysis as an indicator of early glaucomatous damage, CUROP, 2013.

Albon J, Duance VC, £5000, 3D reconstruction of the Human Optic Nerve Head, Thermofisher Inspire Award, 2013.

Albon J, Boote J, Meek K, £39035, Connective tissue in the human optic nerve heads: how does spatial distribution influence its ability to support nerve fibres that pass through it and susceptibility to develop glaucoma. STFC.

Albon J, Boote J, Meek K. £39035, Connective tissue in the human optic nerve head: does regional variation influence its ability to support nerve fibres that pass through it and susceptibility to develop glaucoma? STFC 2012.

Albon J, Boote J, Meek K, £43489, Nanostructure of connective tissue in the human optic nerve head. STFC, 2011.

Albon J, Boote C, Meek K, £43489, How does connective tissue in the human optic nerve head influence its ability to support nerve fibres that pass through it and susceptibility to develop glaucoma? STFC, 2010.

Albon J, Meek K, Boote C, White N. What age-related parameters make an optic nerve head more susceptible to axonal loss and potential blindness? BBSRC DTG studentship, 2009-2013.

Quantock AJ and co-applicants including Albon J, £212,040 BBSRC Doctoral Training Grant, 2009-16.

Albon J, North RV, Drexler W, Morgan JE, £15,000, Optic nerve head changes in the progression of glaucomatous optic neuropathy using 1mm OCT, Hirsch and Fight for Sight Award, 2010-11.

North R, Albon J, Drexler W, £20,000. Three dimensional imaging of the ageing and glaucomatous optic nerve head: risk factors for the development of open-angle glaucoma. UK&Eire Glaucoma Society and International Glaucoma Association, 2008-09.

Morgan JE, Tudor D, Albon J, £49,918, Perineuronal net (PNN) digestion as a therapy for the restoration of retinal ganglion cell structure in glaucoma. National Eye Research Centre, 2010-11.

Morgan JE, Albon J, £49,911, Characterisation of the perineuronal net (PNN) in the glaucomatous human retina: the identification of targets for PNN digestion as a potential therapy for the restoration of retinal ganglion cell structure in glaucoma, International Glaucoma Association, 2010-11.

Albon J, Boote J, Meek K. Connective tissue in the human optic nerve head, STFC funds for 8 days SAXS beamtime at Diamond synchatron, Aug 2013, Mar 2012, Aug 2011, Dec 2010.

Wride MA, Morgan JE, Albon J, £49,918, The role of inhibitors of apoptosis (IAPs) and caspases in retinal ganglion cell death and dendrite remodelling. National Eye Research Centre, 2007-10.

Albon J, Wride MA, Boulton ME, £49,800, Identification of candidate genes critical to epithelial cell patterning and differentiation during corneal development, National Eye Research Centre. 2005-08.

Research Team:

Dr Katharine Mortlock, BrightFocus Foundation (previously American Health Assistance Foundation), 2011-13.

Miss Hannah Jones, Thermofischer Inspire Award, 2013.

Mrs Beth Flynn, College of Optometrists, 2011-2014.

Research Collaborators:

Dr Graham Davies, Institute of Dentistry (School of Medicine and Dentistry), QMUL, London.

Professor Wolfgang Drexler, Center for Medical Physics and Biomedical Engineering, Medical University Vienna.

Professor C. Ross Ethier, Wallace H. Coulter Department of Biomedical Engineering at Georgia Institute of Technology & Emory University School of Medicine London & Georgia Institute of Technology, US.

Mike Fautsch, Department of Ophthalmology, Mayo Graduate School of Medicine, Rochester, US.

Dr Michael Girard, Department of Biomedical Engineering, National University of Singapore, Singapore

Professor Tim Wess, Charles Sturt University, Australia.

Undergraduate Teaching

I have been a Module leader for 3 modules: Second year modules; Diagnostic Techniques and Pathology (2002-2005) and Optometric Science Practicals (2001-2005) and first year module; Cells to Systems (2004-present) in the BSc Optometry and Vision Sciences.

In Cells to System module, I provide lectures on cells, body systems and pathology, and also organise and supervise laboratory practical sessions and tutorials on adult stem cells, ageing, ocular pathology and wound healing.

Postgraduate

I have supervised/co-supervised 18 PhD students to successful award of PhD, and supervise WOPEC MSc Research Projects. I have examined a number of yearly continuation reports, examined a number of PhD students as an internal examiner, chaired PhD vivas and examined MSc thesis for UCL, Dublin, Ireland and PhD thesis for the University of Birmingham.

Educational and Professional Qualifications:

1990-1995 PhD Investigation into the age-related changes in the extracellular matrix of the human lamina cribrosa. Bristol University.

1989 BSc Applied Biological Sciences, Bristol Polytechnic.

Academic Positions:

2000 $acirc; present Lecturer, School of Optometry and Vision Sciences, Cardiff University

1999-2000 Post-doctoral research associate, Cardiff University

1995-1999 Senior Clinical Scientist, Eye Bank, Manchester Healthcare Trust.

Honorary Lecturer, Department of Ophthalmology, Manchester University.

School Roles and Committees:

School and University Roles and Committees:

Director of School Research Audit Ethics Committee
Person designated for School of Optometry and Vision Science (OPTOM), as per Cardiff University Human Tissue Act (HTA) licence
OPTOM Biological Safety Officer
OPTOM Erasmus co-ordinator
Deputy Chair of Teaching and Learning
Histology Lead for VSBL
Optic Nerve Head Group Leader

School Committee Membership:

Safety committee
Research Ethics Audit committee
Teaching and Learning committee
Research committee
Postgraduate research sub-committee
BSc Board of studies
MSc Board of Studies
Vision Science Bioimaging Laboratories committee

University Committee Membership

Cardiff University Genetic Modification Safety Committee
University Human Tissue Act working group
Cardiff Institute of Tissue Engineering and Repair Research Committee (2010-2013)

External Committees/Memberships:

Member of the Association for Research and Vision Sciences and Ophthalmology (ARVO)

Member of the British Society of Matrix Biology

Member of the UK and Eire Glaucoma Society

Member of European Biomechanics Society

Innovation and Engagement:

Registered STEMnet ambassador
Cardiff University Brain Games 2013, National Museum, Cardiff.
Monmouth Science Initiation: The Front & Back of the Eye, Mar and Nov 2013
Monmouth Science Initiation: The Front & Back of the Eye, Mar 2014
Monmouth Science Initiation: In the Blink of an Eye,$acirc; 2010 and 2011
Visual Neuroscience Research Institute neuroscience activities, Techniquest, 2009.

Invited:

The optic nerve in 3D: Connective tissue in the young and old; ARVO 2013 symposium: Architecture of the Eye.

Moderator of Biomechanics paper session, ARVO 2013

Anrhydeddau a dyfarniadau

Outstanding Contribution Award, 2016

Cardiff University Research Fellowship, 2015-2016

Aelodaethau proffesiynol

Member of the Association for Research and Vision Sciences and Ophthalmology (ARVO)
Member of the British Society of Matrix Biology
Member of the UK and Eire Glaucoma Society
Member of European Biomechanics Society

Safleoedd academaidd blaenorol

1999 - 2000 Post-doctoral research associate, Cardiff University
1995 - 1999 Senior Clinical Scientist, Eye Bank, Manchester Healthcare Trust
Honorary Lecturer, Department of Ophthalmology, Manchester University

Pwyllgorau ac adolygu

School university membership

Director of School Research Audit Ethics Committee
Cardiff University Genetic Modification Safety Committee
University Human Tissue Act working group
Cardiff Institute of Tissue Engineering and Repair Research Committee (2010-2013)

School committee membership

Safety committee
Research Ethics Audit committee
Teaching and Learning committee
Research committee
Postgraduate research sub-committee
BSc Board of studies
MSc Board of Studies
Vision Science Bioimaging Laboratories committee

I have successfully supervised 18 PhD students, all of whom have successfully completed their submissions and passed. Students interested in doing PhDs within the group should have an interest in the areas of:

Optic nerve head imaging and image feature analyses
Elastin in the ageing and disease
Collagen in the ageing and disease
Connective tissue micromechanics
Connective tissue and biomechanical properties

Contact Details

AlbonJ@caerdydd.ac.uk
+44 29208 75427
Optometreg a Gwyddorau'r Golwg , Ystafell Room 2.29, Maindy Road, Heol Maendy, Cathays, Caerdydd, CF24 4HQ