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Dr Cedric Berger
(e/fe)
PhD
- Ar gael fel goruchwyliwr ôl-raddedig
Timau a rolau for Cedric Berger
Uwch Ddarlithydd
Ysgol y Biowyddorau
Trosolwg
Mae'r tîm yn gysylltiedig â'r grŵp Microbiomau, Microbau a Gwybodeg (MMI) yn Ysgol y Biowyddorau Caerdydd. Rydym yn astudio'r rhyngweithio rhwng pathogenau enterig a'u gwesteion gyda phwyslais penodol ar Enterohemorrhagig (EHEC), Enteropathogenig (EPEC), Escherichia coli (DAEC) ac Edwardsiella spp. Mae ein hymchwil yn cwmpasu bioleg, imiwnoleg ac atal clefydau enterig.
Mae gan fy nhîm bedwar maes thematig:
1- Deall epidemioleg clefyd dolur rhydd yn y DU ac mewn gwledydd ag incwm isel.
Gan fod y rhan fwyaf o'r bobl sy'n dioddef o ddolur rhydd yn hunan-feddyginiaethu ac nad ydynt yn adrodd eu symptomau i weithwyr iechyd proffesiynol, mae gwir achosion o glefydau dolur rhydd yn y DU yn dal i gael ei ddeall yn wael.
2- Archwilio'r berthynas â pathogen enterig a'r amgylchedd.
Mae clefyd dolur rhydd wedi bod yn gysylltiedig ers amser maith â dŵr halogedig gyda'r prif syniad, os ydym yn rhoi dŵr yfed glân, bydd effaith clefyd dolur rhydd yn gostwng mewn gwledydd ag incwm isel. Fodd bynnag, mae hyn wedi'i brofi'n anghywir. Yn ogystal, mae amlder clefyd dolur rhydd mewn gwledydd ag incwm uchel yn dal i fod yn uchel iawn gyda chyfartaledd o un episod bob blwyddyn ar gyfartaledd sy'n awgrymu bod elfennau eraill yn cael eu trosglwyddo'r pathogenau. Y nod yw deall o ble mae'r pathogenau yn dod yn yr amgylchedd a'u canfod.
3- Decrypther the molecular mechanism of the virulence factor:
Rydym yn gweithio ar sawl system pathogen gwesteiwr-bacteriol sy'n cyfuno ystod eang o dechnegau a dulliau o brofion celloedd in vitro i fodelau in vivo gyda'r nod o ddeall mecanwaith moleciwlaidd ffactorau virulence bacteriol a nodi eu targedau yn y gwesteiwr. Gan y gall haint arwain at ymatebion annormal sy'n gysylltiedig â llid cronig (clefydau llidiol y coluddyn) a chanserau colonig, rydym yn edrych ar y mecanwaith sy'n arwain at y ddau gyflwr.
4- Ymchwilio i ffyrdd newydd o driniaeth ac atal i leihau effaith haint enterig.
Nod y prosiect hwn yw datblygu brechlyn y gellid ei ddefnyddio mewn plant i atal datblygiad clefydau neu mewn anifeiliaid i newid gwladychu EPEC ac EHEC. Rydym hefyd yn ymchwilio i'r berthynas rhwng dolur rhydd ac atodiad sinc.
Mae fy ngrŵp yn gysylltiedig ag Ymchwil Caerdydd i Heintiau a Pharasitiaid mewn Systemau Ecolegol (CRIPES) a gyda
Sefydliad Ymchwil Dŵr.
Gallwch hefyd ddod o hyd i'n gwybodaeth am gyhoeddiadau ar ResearchGate a chofnod wedi'i wirio o adolygiadau cyfnodolion ar Publons.
Cyhoeddiad
2024
- Thomas, G. A. et al., 2024. From field to plate: How do bacterial enteric pathogens interact with ready-to-eat fruit and vegetables, causing disease outbreaks?. Food Microbiology 117 104389. (10.1016/j.fm.2023.104389)
2021
- Chatterjee, S. et al., 2021. The type III secretion system effector EspO of enterohaemorrhagic Escherichia coli inhibits apoptosis through an interaction with HAX-1. Cellular Microbiology 23 (9) e13366. (10.1111/cmi.13366)
- Gharpure, R. et al., 2021. A one health approach to child stunting: evidence and research agenda. American Journal of Tropical Medicine and Hygiene 104 (5), pp.1620-1624. (10.4269/ajtmh.20-1129)
2019
- Prendergast, A. J. et al., 2019. Putting the "A" into WaSH: A call for integrated management of water, animals, sanitation and hygiene. Lancet Global Health 3 (8) Pe336-e337. (10.1016/S2542-5196(19)30129-9)
2018
- Berger, C. N. et al. 2018. The Citrobacter rodentium type III secretion system effector EspO affects mucosal damage repair and antimicrobial responses. PLoS Pathogens 14 (10) e1007406. (10.1371/journal.ppat.1007406)
- Pollard, D. J. et al., 2018. Broad-spectrum regulation of nonreceptor tyrosine kinases by the bacterial ADP-ribosyltransferase EspJ. mBio 9 (2) e00170-18. (10.1128/mBio.00170-18)
2017
- Berger, C. N. et al. 2017. Citrobacter rodentium subverts ATP flux and cholesterol homeostasis in intestinal epithelial cells in vivo. Cell Metabolism 26 (5), pp.738-752.e6. (10.1016/j.cmet.2017.09.003)
- Cepeda-Molero, M. et al., 2017. Attaching and effacing (A/E) lesion formation by enteropathogenic E. coli on human intestinal mucosa is dependent on non-LEE effectors. PLoS Pathogens 13 (10) e1006706. (10.1371/journal.ppat.1006706)
- Sandu, P. et al., 2017. The enterohemorrhagic escherichia coli effector EspW triggers actin remodeling in a Rac1-dependent manner. Infection and Immunity 85 (9) e00244-17. (10.1128/IAI.00244-17)
- Pallett, M. A. et al., 2017. Bacterial virulence factor inhibits caspase-4/11 activation in intestinal epithelial cells. Mucosal Immunology 10 , pp.602-612. (10.1038/mi.2016.77)
- Johnson, R. et al., 2017. The Type III secretion system effector SptP of salmonella enterica serovar typhi. Journal of Bacteriology 199 (4) e00647-16. (10.1128/JB.00647-16)
2016
- Pollard, D. J. et al., 2016. The Type III secretion system effector SeoC of salmonella enterica subsp. salamae and S. enterica subsp. arizonae ADP-Ribosylates Src and Inhibits Opsonophagocytosis. Infection and Immunity 84 (12), pp.3618-3628. (10.1128/IAI.00704-16)
2015
- Hunter, P. J. et al., 2015. Older leaves of lettuce (Lactuca spp.) support higher levels of Salmonella enterica ser. Senftenberg attachment and show greater variation between plant accessions than do younger leaves. FEMS Microbiology Letters 362 (11), pp.1-7. (10.1093/femsle/fnv077)
2014
- Pallett, M. A. et al., 2014. The type III secretion effector NleF of enteropathogenic escherichia coli activates NF-κB early during infection. Infection and Immunity 82 (11), pp.4878-4888. (10.1128/IAI.02131-14)
2013
- Clements, A. et al., 2013. Type 3 secretion effectors. In: Escherichia Coli: Pathotypes and Principles of Pathogenesis 2nd ed.. Academic Pess. , pp.451-497. (10.1016/B978-0-12-397048-0.00015-2)
2012
- Berger, C. N. et al. 2012. EspZ of enteropathogenic and enterohemorrhagic Escherichia coli regulates Type III secretion system protein translocation. mBio 3 (5) e00317-12. (10.1128/mBio.00317-12)
2011
- Shaw, R. K. et al., 2011. Cellulose mediates attachment of Salmonella enterica Serovar Typhimurium to tomatoes. Environmental Microbiology Reports 3 (5), pp.569-573. (10.1111/j.1758-2229.2011.00263.x)
- Robinson, K. S. et al., 2011. Bax Inhibitor 1 in apoptosis and disease. Oncogene 30 (21), pp.2391-2400. (10.1038/onc.2010.636)
- Berger, C. N. et al. 2011. Salmonella enterica strains belonging to O serogroup 1,3,19 induce chlorosis and wilting of Arabidopsis thaliana leaves. Environmental Microbiology 13 (5), pp.1299-1308. (10.1111/j.1462-2920.2011.02429.x)
- Shaw, R. K. et al., 2011. Flagella mediate attachment of enterotoxigenic Escherichia coli to fresh salad leaves. Environmental Microbiology Reports 3 (1), pp.112-117. (10.1111/j.1758-2229.2010.00195.x)
2010
- Martinez, E. et al., 2010. Binding to Na+/H+ exchanger regulatory factor 2 (NHERF2) affects trafficking and function of the enteropathogenic Escherichia coli type III secretion system effectors Map, EspI and NleH. Cellular Microbiology 12 (12), pp.1718-1731. (10.1111/j.1462-5822.2010.01503.x)
- Berger, C. N. et al. 2010. Fresh fruit and vegetables as vehicles for the transmission of human pathogens. Environmental Microbiology 12 (9), pp.2385-2397. (10.1111/j.1462-2920.2010.02297.x)
- Robinson, K. S. et al., 2010. The enteropathogenic Escherichia coli effector NleH inhibits apoptosis induced by Clostridium difficile toxin B. Microbiology 156 (6), pp.1815-1823. (10.1099/mic.0.037259-0)
- Arbeloa, A. et al., 2010. EspM2 is a RhoA guanine nucleotide exchange factor. Cellular Microbiology 12 (5), pp.654-664. (10.1111/j.1462-5822.2009.01423.x)
- Bulgin, R. et al., 2010. Bacterial guanine nucleotide exchange factors SopE-Like and WxxxE effectors. Infection and Immunity 78 (4), pp.1417-1425. (10.1128/IAI.01250-09)
- Hemrajani, C. et al., 2010. NleH effectors interact with Bax inhibitor-1 to block apoptosis during enteropathogenic Escherichia coli infection. Proceedings of the National Academy of Sciences 107 (7), pp.3129-3134. (10.1073/pnas.0911609106)
2009
- Frankel, G. et al., 2009. Fresh produce as a potential vector for bacterial human pathogens. Microbial Biotechnology -Blackwell- 2 (6), pp.595-597. (10.1111/j.1751-7915.2009.00137.x)
- Berger, C. N. et al. 2009. Interaction of enteroaggregative Escherichia coli with salad leaves. Environmental Microbiology Reports 1 (4), pp.234-239. (10.1111/j.1758-2229.2009.00037.x)
- Berger, C. N. et al. 2009. The mechanisms used by enteropathogenic Escherichia coli to control filopodia dynamics. Cellular Microbiology 11 (2), pp.309-322. (10.1111/j.1462-5822.2008.01254.x)
- Berger, C. N. et al. 2009. Interaction of Salmonella enterica with basil and other salad leaves. ISME Journal 3 (2), pp.261-265. (10.1038/ismej.2008.95)
2008
- Hemrajani, C. et al., 2008. Role of NleH, a Type III secreted effector from attaching and effacing pathogens, in colonization of the bovine, ovine, and murine gut. Infection and Immunity 76 (11), pp.4804-4813. (10.1128/IAI.00742-08)
- Arbeloa, A. et al., 2008. Subversion of actin dynamics by EspM effectors of attaching and effacing bacterial pathogens. Cellular Microbiology 10 (7), pp.1429-1441. (10.1111/j.1462-5822.2008.01136.x)
- Shaw, R. K. et al., 2008. Enterohemorrhagic escherichia coli exploits EspA filaments for attachment to salad leaves. Applied and Environmental Microbiology 74 (9), pp.2908-2914. (10.1128/AEM.02704-07)
- Rougeaux, C. , Berger, C. N. and Servin, A. L. 2008. hCEACAM1-4L downregulates hDAF-associated signalling after being recognized by the Dr adhesin of diffusely adhering Escherichia coli. Cellular Microbiology 10 (3), pp.632-654. (10.1111/j.1462-5822.2007.01072.x)
2007
- Fayol-Messaoudi, D. et al., 2007. The Lactobacillus plantarum strain ACA-DC287 isolated from a Greek cheese demonstrates antagonistic activity in vitro and in vivo against Salmonella enterica serovar Typhimurium. Journal of Applied Microbiology 103 (3), pp.657-665. (10.1111/j.1365-2672.2007.03293.x)
2005
- Fayol-Messaoudi, D. et al., 2005. pH-, lactic acid-, and non-lactic acid-dependent activities of probiotic lactobacilli against salmonella enterica serovar typhimurium. Applied and Environmental Microbiology 71 (10), pp.6008-6013. (10.1128/AEM.71.10.6008-6013.2005)
2004
- Kansau, I. et al., 2004. Zipper-like internalization of Dr-Positive Escherichia coli by epithelial cells is preceded by an adhesin-induced mobilization of raft-associated molecules in the initial step of adhesion. Infection and Immunity 72 (7), pp.3733-3742. (10.1128/IAI.72.7.3733-3742.2004)
- Berger, C. N. et al. 2004. Differential recognition of members of the carcinoembryonic antigen family by Afa/Dr adhesins of diffusely adhering Escherichia coli (Afa/Dr DAEC). Molecular Microbiology 52 (4), pp.963-983. (10.1111/j.1365-2958.2004.04033.x)
- Vuyst, L. D. et al., 2004. Antimicrobial potential of probiotic or potentially probiotic lactic acid bacteria, the first results of the international European research project PROPATH of the PROEUHEALTH cluster. Microbial Ecology in Health and Disease 16 (2-3), pp.125-130. (10.1080/08910600410032303)
Adrannau llyfrau
- Clements, A. et al., 2013. Type 3 secretion effectors. In: Escherichia Coli: Pathotypes and Principles of Pathogenesis 2nd ed.. Academic Pess. , pp.451-497. (10.1016/B978-0-12-397048-0.00015-2)
Erthyglau
- Thomas, G. A. et al., 2024. From field to plate: How do bacterial enteric pathogens interact with ready-to-eat fruit and vegetables, causing disease outbreaks?. Food Microbiology 117 104389. (10.1016/j.fm.2023.104389)
- Chatterjee, S. et al., 2021. The type III secretion system effector EspO of enterohaemorrhagic Escherichia coli inhibits apoptosis through an interaction with HAX-1. Cellular Microbiology 23 (9) e13366. (10.1111/cmi.13366)
- Gharpure, R. et al., 2021. A one health approach to child stunting: evidence and research agenda. American Journal of Tropical Medicine and Hygiene 104 (5), pp.1620-1624. (10.4269/ajtmh.20-1129)
- Prendergast, A. J. et al., 2019. Putting the "A" into WaSH: A call for integrated management of water, animals, sanitation and hygiene. Lancet Global Health 3 (8) Pe336-e337. (10.1016/S2542-5196(19)30129-9)
- Berger, C. N. et al. 2018. The Citrobacter rodentium type III secretion system effector EspO affects mucosal damage repair and antimicrobial responses. PLoS Pathogens 14 (10) e1007406. (10.1371/journal.ppat.1007406)
- Pollard, D. J. et al., 2018. Broad-spectrum regulation of nonreceptor tyrosine kinases by the bacterial ADP-ribosyltransferase EspJ. mBio 9 (2) e00170-18. (10.1128/mBio.00170-18)
- Berger, C. N. et al. 2017. Citrobacter rodentium subverts ATP flux and cholesterol homeostasis in intestinal epithelial cells in vivo. Cell Metabolism 26 (5), pp.738-752.e6. (10.1016/j.cmet.2017.09.003)
- Cepeda-Molero, M. et al., 2017. Attaching and effacing (A/E) lesion formation by enteropathogenic E. coli on human intestinal mucosa is dependent on non-LEE effectors. PLoS Pathogens 13 (10) e1006706. (10.1371/journal.ppat.1006706)
- Sandu, P. et al., 2017. The enterohemorrhagic escherichia coli effector EspW triggers actin remodeling in a Rac1-dependent manner. Infection and Immunity 85 (9) e00244-17. (10.1128/IAI.00244-17)
- Pallett, M. A. et al., 2017. Bacterial virulence factor inhibits caspase-4/11 activation in intestinal epithelial cells. Mucosal Immunology 10 , pp.602-612. (10.1038/mi.2016.77)
- Johnson, R. et al., 2017. The Type III secretion system effector SptP of salmonella enterica serovar typhi. Journal of Bacteriology 199 (4) e00647-16. (10.1128/JB.00647-16)
- Pollard, D. J. et al., 2016. The Type III secretion system effector SeoC of salmonella enterica subsp. salamae and S. enterica subsp. arizonae ADP-Ribosylates Src and Inhibits Opsonophagocytosis. Infection and Immunity 84 (12), pp.3618-3628. (10.1128/IAI.00704-16)
- Hunter, P. J. et al., 2015. Older leaves of lettuce (Lactuca spp.) support higher levels of Salmonella enterica ser. Senftenberg attachment and show greater variation between plant accessions than do younger leaves. FEMS Microbiology Letters 362 (11), pp.1-7. (10.1093/femsle/fnv077)
- Pallett, M. A. et al., 2014. The type III secretion effector NleF of enteropathogenic escherichia coli activates NF-κB early during infection. Infection and Immunity 82 (11), pp.4878-4888. (10.1128/IAI.02131-14)
- Berger, C. N. et al. 2012. EspZ of enteropathogenic and enterohemorrhagic Escherichia coli regulates Type III secretion system protein translocation. mBio 3 (5) e00317-12. (10.1128/mBio.00317-12)
- Shaw, R. K. et al., 2011. Cellulose mediates attachment of Salmonella enterica Serovar Typhimurium to tomatoes. Environmental Microbiology Reports 3 (5), pp.569-573. (10.1111/j.1758-2229.2011.00263.x)
- Robinson, K. S. et al., 2011. Bax Inhibitor 1 in apoptosis and disease. Oncogene 30 (21), pp.2391-2400. (10.1038/onc.2010.636)
- Berger, C. N. et al. 2011. Salmonella enterica strains belonging to O serogroup 1,3,19 induce chlorosis and wilting of Arabidopsis thaliana leaves. Environmental Microbiology 13 (5), pp.1299-1308. (10.1111/j.1462-2920.2011.02429.x)
- Shaw, R. K. et al., 2011. Flagella mediate attachment of enterotoxigenic Escherichia coli to fresh salad leaves. Environmental Microbiology Reports 3 (1), pp.112-117. (10.1111/j.1758-2229.2010.00195.x)
- Martinez, E. et al., 2010. Binding to Na+/H+ exchanger regulatory factor 2 (NHERF2) affects trafficking and function of the enteropathogenic Escherichia coli type III secretion system effectors Map, EspI and NleH. Cellular Microbiology 12 (12), pp.1718-1731. (10.1111/j.1462-5822.2010.01503.x)
- Berger, C. N. et al. 2010. Fresh fruit and vegetables as vehicles for the transmission of human pathogens. Environmental Microbiology 12 (9), pp.2385-2397. (10.1111/j.1462-2920.2010.02297.x)
- Robinson, K. S. et al., 2010. The enteropathogenic Escherichia coli effector NleH inhibits apoptosis induced by Clostridium difficile toxin B. Microbiology 156 (6), pp.1815-1823. (10.1099/mic.0.037259-0)
- Arbeloa, A. et al., 2010. EspM2 is a RhoA guanine nucleotide exchange factor. Cellular Microbiology 12 (5), pp.654-664. (10.1111/j.1462-5822.2009.01423.x)
- Bulgin, R. et al., 2010. Bacterial guanine nucleotide exchange factors SopE-Like and WxxxE effectors. Infection and Immunity 78 (4), pp.1417-1425. (10.1128/IAI.01250-09)
- Hemrajani, C. et al., 2010. NleH effectors interact with Bax inhibitor-1 to block apoptosis during enteropathogenic Escherichia coli infection. Proceedings of the National Academy of Sciences 107 (7), pp.3129-3134. (10.1073/pnas.0911609106)
- Frankel, G. et al., 2009. Fresh produce as a potential vector for bacterial human pathogens. Microbial Biotechnology -Blackwell- 2 (6), pp.595-597. (10.1111/j.1751-7915.2009.00137.x)
- Berger, C. N. et al. 2009. Interaction of enteroaggregative Escherichia coli with salad leaves. Environmental Microbiology Reports 1 (4), pp.234-239. (10.1111/j.1758-2229.2009.00037.x)
- Berger, C. N. et al. 2009. The mechanisms used by enteropathogenic Escherichia coli to control filopodia dynamics. Cellular Microbiology 11 (2), pp.309-322. (10.1111/j.1462-5822.2008.01254.x)
- Berger, C. N. et al. 2009. Interaction of Salmonella enterica with basil and other salad leaves. ISME Journal 3 (2), pp.261-265. (10.1038/ismej.2008.95)
- Hemrajani, C. et al., 2008. Role of NleH, a Type III secreted effector from attaching and effacing pathogens, in colonization of the bovine, ovine, and murine gut. Infection and Immunity 76 (11), pp.4804-4813. (10.1128/IAI.00742-08)
- Arbeloa, A. et al., 2008. Subversion of actin dynamics by EspM effectors of attaching and effacing bacterial pathogens. Cellular Microbiology 10 (7), pp.1429-1441. (10.1111/j.1462-5822.2008.01136.x)
- Shaw, R. K. et al., 2008. Enterohemorrhagic escherichia coli exploits EspA filaments for attachment to salad leaves. Applied and Environmental Microbiology 74 (9), pp.2908-2914. (10.1128/AEM.02704-07)
- Rougeaux, C. , Berger, C. N. and Servin, A. L. 2008. hCEACAM1-4L downregulates hDAF-associated signalling after being recognized by the Dr adhesin of diffusely adhering Escherichia coli. Cellular Microbiology 10 (3), pp.632-654. (10.1111/j.1462-5822.2007.01072.x)
- Fayol-Messaoudi, D. et al., 2007. The Lactobacillus plantarum strain ACA-DC287 isolated from a Greek cheese demonstrates antagonistic activity in vitro and in vivo against Salmonella enterica serovar Typhimurium. Journal of Applied Microbiology 103 (3), pp.657-665. (10.1111/j.1365-2672.2007.03293.x)
- Fayol-Messaoudi, D. et al., 2005. pH-, lactic acid-, and non-lactic acid-dependent activities of probiotic lactobacilli against salmonella enterica serovar typhimurium. Applied and Environmental Microbiology 71 (10), pp.6008-6013. (10.1128/AEM.71.10.6008-6013.2005)
- Kansau, I. et al., 2004. Zipper-like internalization of Dr-Positive Escherichia coli by epithelial cells is preceded by an adhesin-induced mobilization of raft-associated molecules in the initial step of adhesion. Infection and Immunity 72 (7), pp.3733-3742. (10.1128/IAI.72.7.3733-3742.2004)
- Berger, C. N. et al. 2004. Differential recognition of members of the carcinoembryonic antigen family by Afa/Dr adhesins of diffusely adhering Escherichia coli (Afa/Dr DAEC). Molecular Microbiology 52 (4), pp.963-983. (10.1111/j.1365-2958.2004.04033.x)
- Vuyst, L. D. et al., 2004. Antimicrobial potential of probiotic or potentially probiotic lactic acid bacteria, the first results of the international European research project PROPATH of the PROEUHEALTH cluster. Microbial Ecology in Health and Disease 16 (2-3), pp.125-130. (10.1080/08910600410032303)
Ymchwil
Research
We are working on several host-bacterial pathogen systems combining a wide range of techniques and approaches from in-vitro cell assays to in vivo models. The aim of my group is to understand the molecular mechanism of bacterial virulence factors and identify their targets in the host.
Modulation of immune response
Escherichia coli is the major constitute of the aerobic gut microbiota. However, acquisition of virulence determinant by horizontal gene transfer led to the evolution of pathogenic E. coli clones. Pathogenic strains are divided into ExPEC (causing extra intestinal infection) and diarrhoeal E. coli, which are sub-divided into six categories: diffusely adherent (DAEC), entero-toxigenic (ETEC), -aggregative (EAEC), -invasive (EIEC), -pathogenic (EPEC) and -haemorrhagic (EHEC). Although EPEC and EHEC exhibit differences in their host specificity, epidemiology, virulence and disease symptoms, they colonise the gut mucosa of their respective hosts via attaching and effacing (A/E) lesions. A/E lesions are characterised by effacement of the brush border microvilli and intimate bacterial attachment to the enterocyte membrane (Figure 2). The ability to induce A/E lesions and colonize their hosts depend on a type III secretion system (T3SS) that injects effectors into colonic intestinal epithelial cells (IECs). Once injected, the effectors subvert processes involved in innate immune responses, cellular metabolism, and oxygenation of the mucosa. We recently reported that one of these effectors modulates the recruitment of neutrophils during mouse model of EPEC infection (Citrobacter rodentium). We are now focusing on other effectors that may target key signalling pathways that lead to the expression of cytokines.
Disease prevention
Different vaccinations, composed of virulence factors, DNA-based, attenuated bacteria or bacterial ghost against EPEC and EHEC, have been trialled in animals with mixed success. Vaccination against Intimin decreases EPEC colonization in the rabbit model. Vaccination targeting EspA, Tir and Intimin alters EHEC colonization in cattle but did not completely inhibit it. This vaccination was one of the two commercially available against EHEC, EconicheTM, but its production has stopped. The main problems of all these vaccinations is the diversity of the target among EPEC and EHEC suggesting that a single approach will not work.
The aim of this project is to develop a vaccine that could be used in children to prevent disease development or in animals to alter the colonization of EPEC and EHEC using C. rodentium, a mouse specific pathogen, used to study EPEC and EHEC infection in vivo as it shares the same virulence factors.
Current members of the Research Group
Technical staff:
Gareth Thomas working on plant contamination by Listeria
Master students:
Doyle Millard working on cell signalling induce by enteric pathogens
Undergraduate research students:
None
Addysgu
Rwy'n cynnal ystod o addysgu o flwyddyn 1 i flwyddyn olaf israddedig, a goruchwylio myfyrwyr prosiect MRes blwyddyn olaf ac ôl-raddedig.
Mae fy addysgu presennol yn cynnwys cyfraniadau i:
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BI1001: Sgil ar gyfer Gwyddoniaeth - Datrys Problemau mewn Bioleg ac ymarfer ar dechnegau aseptig.
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BI1003: Organebau a'r Amgylchedd - Microbioleg feddygol
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BI3155: Bioleg ac epidemioleg heintiau - Ymatebion gwesteiwr i haint (Arweinydd modiwl)
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BI4001: Prosiect ymchwil meistr integredig (Arweinydd y modiwl)
Bywgraffiad
For almost 20 years I have been interested in bacterial infection and methods to prevent them. My PhD project at University Paris Sud at the faculty of Pharmacy in France focused on the interaction between Diffusely adherent Escherichia coli and the human epithelial cells. In 2006, I then moved to Imperial College London and developed my expertise on biomedical microbiology focusing on the human pathogens Enteropathogenic and Enterohemorrhagic E. coli and the mouse-restricted pathogen Citrobacter rodentium. In September 2018, I joined Cardiff School of Biosciences, Cardiff University, as a Senior Lecturer, to carry on my work on pathogenic bacteria.
Aelodaethau proffesiynol
Member of The Microbiology Society and American Society for Microbiology
Meysydd goruchwyliaeth
Profwr
Goruchwyliaeth gyfredol
Prosiectau'r gorffennol
- Teresa Paradel Gil (pathogenau A/E)
- Nicolaas Bruyniks (Listeria monocytogenes
Contact Details
+44 29208 74829
Adeilad Syr Martin Evans, Llawr 2, Ystafell Ystafell W/2.06, Rhodfa'r Amgueddfa, Caerdydd, CF10 3AX
Themâu ymchwil
Arbenigeddau
- Afiechydon heintus
- Genomeg pathogen
- Rhyngweithio gwesteiwr-pathogenau
- Microbioleg Bwyd
- microbiome