Ewch i’r prif gynnwys
Paola Borri

Yr Athro Paola Borri

Yr Athro, Cydlynydd Consortiwm ETN Marie Curie Ewropeaidd MUSIQ

Ysgol y Biowyddorau

Email
BorriP@caerdydd.ac.uk
Telephone
+44 29208 79356
Campuses
Adeilad Syr Martin Evans, Ystafell Cardiff School of Biosciences, The Sir Martin Evans Building, Museum Avenue, Cardiff, CF10 3AX, Rhodfa'r Amgueddfa, Caerdydd, CF10 3AX
Users
Ar gael fel goruchwyliwr ôl-raddedig

Trosolwyg

Mae gweithgareddau ymchwil yn fy ngrŵp yn cwmpasu maes bioffotoneg yn y rhyngwyneb rhwng bywyd a'r gwyddorau ffisegol. Mewn cydweithrediad â'r Ysgol Ffiseg a Seryddiaeth, mae fy ngwaith ymchwil yn cynnwys:

  • datblygu microsgopau amlffoton y genhedlaeth nesaf yn seiliedig ar Gwasgariad Raman cydlynol (CRS) ar gyfer astudiaethau di-label mewn celloedd byw a meinweoedd
  • Datblygu technegau microsgopeg optegol newydd ar gyfer delweddu ac olrhain cefndir nanoronynnau bach sengl yn rhydd y tu mewn i gelloedd
  • datblygu biosynwyryddion optegol ar gyfer canfod biomoleciwlau yn sensitif gan ddefnyddio synhwyro plasmoneg gyda nanoronynnau metelaidd neu geudodau ffotonig
  • Trosglwyddo ynni cyseiniant fflworoleuedd wedi'i ddatrys amser (TR-FRET) fel chwiliedydd o ryngweithiadau biomolecwlaidd

Gweler hefyd ein gwefan MUSIQ i gael y wybodaeth ddiweddaraf am yr ymchwil o fewn consortiwm yr UE hwn.

Newyddion

Gweler ein huchafbwyntiau ymchwil a gyhoeddwyd yn ddiweddar:

Oes gennych chi ddiddordeb mewn ymuno â'm labordy fel myfyriwr ôl-raddedig hunan-ariannu neu bostdoc/cymrodyr?  Cysylltwch â mi drwy e-bost.

Cyhoeddiad

2024

2023

2022

2021

2020

2019

2018

2017

2016

2015

2014

2013

2012

2011

2010

2009

2008

2007

2006

2005

2004

2003

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2001

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1999

1998

  • Gurioli, M. et al. 1998. Exciton formation and relaxation in GaAs epilayers. Physical Review B 58(20), pp. R13403-R13406. (10.1103/PhysRevB.58.R13403)
  • Langbein, W. W., Borri, P. and Hvam, J. M. 1998. Coherent exciton and biexciton nonlinearities in semiconductor nanostructures: effects of disorder. Presented at: 10th International Conference on Ultrafast Phenomena in Semiconductors, Vilnius, Lithuania, August-September 1998 Presented at Steponas, A. and Dargys, A. eds.Ultrafast Phenomena in Semiconductors: Proceedings of the 10th International Symposium on Ultrafast Phenomena in Semiconductors (10-UFPS), held in Vilnius, Lithuania, August/September 1998. Materials Science Forum Vol. 297-8. Zurich: Trans Tech Publications pp. 73-78.
  • Borri, P., Langbein, W. W. and Hvam, J. M. 1998. Ultrafast spectroscopy of semiconductor devices. Presented at: 10th International Symposium on Ultrafast Phenomena in Semiconductors, Vilnius, Lithuania, August-September 1998 Presented at Asmontas, S. and Dargys, A. eds.Ultrafast phenomena in semiconductors : proceedings of the 10th International Symposium on Ultrafast Phenomena in Semiconductors (10-UFPS), held in Vilnius, Lithuania, August/September 1998. Materials Science Forum Vol. 297-8. Zurich: Trans TechPublications pp. 67-71.

1997

1996

1995

Adrannau llyfrau

Cynadleddau

Erthyglau

Monograffau

Patentau

Ymchwil

Projectau

Microsgopeg Gwasgariad Raman cydlynol ar gyfer delweddu di-label o gelloedd byw a meinweoedd

Mae microsgopeg optegol yn offeryn anhepgor ar gyfer bioleg celloedd. Mae gwahanol ddulliau microsgopeg ar gael ar hyn o bryd ac mae ymdrech barhaus yn cael ei neilltuo i ddatblygu technegau newydd gyda gwell sensitifrwydd, detholusrwydd a datrysiad gofodol. Mater pwysig mewn microsgopeg diweddar yw'r gallu i berfformio astudiaethau anfewnwthiol gan osgoi'r angen am chwilotwyr fflwroleuol sy'n dueddol o ffotoblethu ac sy'n gallu ymwneud â strwythur a swyddogaeth y gell.

Ynghyd â'r Athro Wolfgang Langbein yn yr Ysgol Ffiseg a Seryddiaeth, rydym wedi datblygu ystod o ficrosgopau amlffoton sganio laser cartref yn seiliedig ar Gwasgariad Raman cydlynol (CRS), gan alluogi microsgopeg cyflym di-label penodol yn gemegol o gelloedd byw a meinweoedd. Mae microsgop CRS ail genhedlaeth sydd wedi'i leoli yn Ysgol y Biowyddorau wedi'i adeiladu'n benodol i berfformio CRS cydberthynas amlfoddol, fflworoleuedd dau ffoton (TPF) ac ail ficrosgopeg cynhyrchu harmonig (SHG) ar gyfer cymwysiadau mewn bioleg celloedd. Mae'r system hon yn galluogi caffaeliad CRS hyperspectral sydd wedi arwain at ddatblygu offeryn dadansoddi delwedd meintiol i wahaniaethu cyfansoddiad cydrannau cemegol ac adfer eu crynodiad a'u dosbarthiad gofodol.

Gyda'r offer hyn, rydym wedi penderfynu ar y defnydd lipid o fôn-gelloedd dynol sy'n deillio o adiposau sefydlog a byw sy'n gwahaniaethu i mewn i gyn-adipocytau, y cynnwys lipid a'r dosbarthiad gofodol mewn oocytes mamalaidd byw ac embryonau cynnar, wedi dangos platfform cynnwys uchel trwybwn ar gyfer sgriniau cyffuriau, a mesur màs meintiol o lipidau, proteinau a DNA yn ystod rhaniad celloedd. Rydym hefyd wedi dangos y gellir defnyddio CRS i ddelweddu nanodiemwntau sengl nad ydynt yn fflworoleuo mewn celloedd am y tro cyntaf (gweler Cyhoeddiadau).

Resonant Cymysgu Pedwar-Ton Delweddu gyda nanoparticles aur

Mae delweddu ac olrhain nanoronynnau sengl gan ddefnyddio microsgopeg optegol yn dechnegau pwerus gyda llawer o gymwysiadau mewn bioleg, cemeg a gwyddorau materol. Mae dulliau cymhwysol i gyflawni cyferbyniad yn seiliedig ar fflworoleuedd dominant, gyda therfynau sylfaenol yn y fflwcs ffoton a allyrrir yn deillio o'r oes gyffrous-wladwriaeth yn ogystal â ffotoblethu.

Rydym wedi datblygu techneg newydd, yn seiliedig ar ryngweitheg cymysgu pedair ton (FWM), lle mae nanoronynnau aur sengl nad ydynt yn fflworoleuo (AuNPs) yn cael eu delweddu'n rhydd o gefndir hyd yn oed y tu mewn amgylcheddau cellog heterogenaidd iawn, oherwydd eu hymateb plasmonig aflinol penodol, ac mae eu sefyllfa'n cael ei bennu gyda manylder nanometrig mewn 3D. Mae'r dechneg hefyd yn unigryw o sensitif i amwynderau gronynnau o ddim ond 0.5% deallusrwydd, sy'n cyfateb i un haen atomig o aur, yn ogystal â chyfeiriadedd gronynnau a chirality.

Gyda'r dechneg hon, rydym yn ymchwilio i nifer o gyfuniadau AuNP-ligand-fflworffore a'u uniondeb y tu mewn i gelloedd, gan ddefnyddio AuNPs mor fach â 5nm mewn radiws a delweddu fflworoleuedd cydberthynol FWM / cydffocwsgop. Mae'r dechneg yn agor y posibilrwydd i lefel ddigynsail o ddealltwriaeth o dynged mewngellol AuNPs bach sengl a'u masnachu o fewn pensaernïaeth 3D gymhleth y tu mewn i gelloedd byw (gweler Cyhoeddiadau).

Diddordebau ymchwil eraill

  • Microsgopeg difodiant optegol meintiol o nano-wrthrychau unigol
  • Deinameg cydlynol Ultrafast o ddeunyddiau a dyfeisiau dot cwantwm lled-ddargludyddion
  • Biosensio optegol trwy ecsbloetio cyseiniannau plasmonig neu geudodau ffotonig 
  • Trosglwyddo Ynni Cyseiniant Förster (TR FRET) wedi'i ddatrys gan amser fel chwiliedydd rhyngweithiadau biomolecwlaidd

Mae ein labordy Bioffotoneg yn siarad am gyfleuster y Ganolfan Ymchwil Bioddelweddu

Grantiau

  • BBSRC, EPSRC, MRC, EU, Y Weinyddiaeth Amddiffyn – Dstl, DTI

Aelodau'r grŵp

  • Iestyn Pope, Cydymaith Ymchwil (Ysgol y Biowyddorau, Caerdydd)
  • Francesco Masia, Cymrawd Ymchwil (Ysgol y Biowyddorau, Caerdydd)
  • David Regan, Cydymaith Ymchwil (Ysgol y Biowyddorau, Caerdydd)
  • Lukas PayneCydymaith Ymchwil (Ysgol Ffiseg a Seryddiaeth, Caerdydd)
  • Rhod Thomasmyfyriwr PhD (Ysgol y Biowyddorau, Caerdydd)
  • Nadhia Monimmyfyriwr PhD (Ysgol y Biowyddorau, Caerdydd)
  • Nicole Slesiona, Cymrawd ESR MUSIQ a myfyriwr PhD (Ysgol y Biowyddorau, Caerdydd)
  • Vikramdeep Singh, Cymrawd ESR MUSIQ a myfyriwr PhD (Ysgol Ffiseg a Seryddiaeth, Caerdydd)
  • Martina RecchiaCymrawd ESR MUSIQ a myfyriwr PhD (Ysgol y Biowyddorau, Caerdydd)
  • Jan Majer, Cymrawd ESR MUSIQ a Myfyriwr PhD (Ysgol y Biowyddorau, Caerdydd)
  • Dominykas GudaviciusCymrawd ESR MUSIQ a Myfyriwr PhD (Ysgol Ffiseg a Seryddiaeth, Caerdydd)
  • Freya TurleyMyfyriwr PhD (Ysgol Ffiseg a Seryddiaeth, Caerdydd)
  • Ozan Aksakalmyfyriwr PhD (Ysgol y Biowyddorau, Caerdydd)
  • Athena Zittimyfyriwr PhD (Ysgol y Biowyddorau, Caerdydd)
  • Emily Lewismyfyriwr PhD (Ysgol y Biowyddorau, Caerdydd)
  • Furqan Alabdullah, myfyriwr PhD (Ysgol Engeneering, Caerdydd)
  • Owen EvansMyfyriwr PhD (Ysgol Ffiseg a Seryddiaeth, Caerdydd)
  • Amit Nilabh, Myfyriwr PhD (Ysgol y Biowyddorau, Caerdydd)
  • Tess Harrisonmyfyriwr PhD (Ysgol y Biowyddorau, Caerdydd)
  • Adam Cutts, myfyriwr PhD (Ysgol y Biowyddorau, Caerdydd)
  • Matthew De Guzman, myfyriwr PhD (Ysgol y Biowyddorau, Caerdydd)
  • Lurui Wanmyfyriwr PhD (Ysgol y Biowyddorau, Caerdydd)
  • Emma Boppmyfyrwraig MPhil  (Ysgol y Biowyddorau, Caerdydd)
  • John McLarnon, myfyriwr PhD (Ysgol y Biowyddorau, Caerdydd)

Cydweithredwyr

Addysgu

Module Lead of 4th-year Intergated Masters module "Advanced Research Methods"

Bywgraffiad

I did my undergraduate in Physics at the University of Florence (Italy) and then received the Diploma and the Ph.D degree in Physics in 1993 and 1997 respectively. From 1997 to 1999 I was Assistant Research Professor at the Technical University of Denmark (Kgs.Lyngby, Denmark).

From 1999 to 2004 I worked as Senior Scientist and EU Marie Curie Fellow (2001-2003) at the Physics Department of Dortmund University in Germany where I received the Habilitation degree in Physics (Venia Legendi) in 2003. During this time I was interested in the experimental investigation of the optical properties of novel semiconductor nanostructures, such as quantum wells and quantum dots. In particular, I developed a new technique for ultra-fast coherent laser spectroscopy of these nanostructures.

From September 2004 I moved to Cardiff University as Senior Lecturer. On August 1st 2007 I was promoted to Reader and on August 1st 2011 to a Personal Chair.

Anrhydeddau a dyfarniadau

In 2015 I received the Royal Society Wolfson Research Merit Award. Jointly funded by the Wolfson Foundation and the Department for Business, Innovation and Skills (BIS), the scheme aims to provide universities with additional support to enable them to attract science talent from overseas and retain respected UK scientists of outstanding achievement and potential.

I received the Marie Curie Excellence Award from the European Commission during the official award ceremony at the Ecole Polytechnique Federale de Lausanne on the 16th November 2006.

Marie Curie Excellence Awards (EXA) aim to give public recognition to outstanding past achievements of scientists who have reached a level of exceptional excellence in their given field. Up to five prizes of 50 000 Euros each are awarded every year.

Aelodaethau proffesiynol

In 2010-2015 I was an EPSRC Leadership Fellow.

Since 2014 I am Fellow of the Learned Society of Wales.

Meysydd goruchwyliaeth

I am interested in supervising PhD students in these research areas:

  • nonlinear laser micro-spectroscopy
  • label-free vibrational microscopy of living cells and tissues
  • imaging and tracking single nanoparticles in living systems
  • lipid membrane biophysics

Project Example:

Title: Shedding new light on single protein-lipid membrane interactions

The interaction between proteins and lipid membranes is a fundamental process underpinning key functions in cell biology and the maintenance of life. Membrane proteins account for approximately 27% of the entire human proteome, and membrane receptors make up the largest group of drug targets in humans since they play a critical role in both infection and immunity [1]. Membranes are also the target for protein toxins produced by pathogens to attack cells from the outside and introduce perforations. Beyond their impact to human disease, toxins are of great interest in biotechnology, to control e.g. insect pests of agriculture [2]. Moreover, the challenge of antimicrobial resistance has ignited strong interest in antimicrobial peptides (AMPs) which form membrane-spanning pores as part of their bactericidal activity [3].

Despite the widespread importance of such systems, many key questions are still unanswered, including how do proteins remodel and diffuse within membranes in space and time? Where do they partition, depending on the heterogeneous lipid membrane chemical composition and curvature? How is the protein function modulated by the lipid environment at the atomic, molecular and long-range meso-scale? How is the lipid membrane local composition and curvature affected by the protein (an interplay often overlooked).

A major roadblock in achieving this understanding is the lack of suitable techniques capable of measuring single protein-lipid membrane interactions at the nanoscale with sub-millisecond time resolution, while keeping the system under observation in its intact natural state and without introducing structural-functional artefacts. Optical microscopy is a promising non-destructive and non-contact technique. However, to achieve the required sensitivity and specificity, presently it relies on tagging proteins and/or lipids, typically with fluorophores, which raises the question if the observed behaviour is real or artefactual [4].

The aim of this project is to contribute to the development and application of novel label-free optical imaging techniques to quantify the diffusion and partitioning of single proteins in physiologically relevant lipid membranes.

[1] https://doi.org/10.1038/nrd3478

[2] https://doi.org/10.3390/toxins6123296

[3] https://doi.org/10.3389/fcimb.2016.00194

[4] http://dx.doi.org/10.1038/nphoton.2015.251

Research Environment:  You will be exposed to a vibrant multi-disciplinary environment at the physics/life science interface.  You will join a well-funded academic team, with an outstanding track record of student supervision and publication output. The supervisory team offers a unique combination of expertise, with strong track records in developing novel optical microscopy techniques applied to life sciences. You will be immersed in a collaborative environment with expertise in the biology of lipid membranes and pore forming proteins, endocytosis and intracellular trafficking.

Training and Development Opportunities: You will be trained in a variety of relevant techniques including advanced optical microscopy methods, fabrication of synthetic lipid membranes, and mammalian cell culture. You will develop the transferable skills of data analysis, communication and dissemination. The resulting skillset will boost your future employability both in academia and in industry. The supervisory team has strong links with companies, including microscope manufactures and image analysis software developers. Within this studentship, opportunities for visits/internships at these companies will arise. Global mobility opportunities will include visiting collaborating partner groups overseas, and participation to national/international conferences. The project will generate new knowledge and data that will be published in high quality journals.

Goruchwyliaeth gyfredol

Adam Cutts

Adam Cutts

Arddangoswr Graddedig

Emma Bopp

Emma Bopp

Myfyriwr ymchwil