![Aled Clayton](https://profiles-cardiff.imgix.net/attachments/2399?w=200&h=200&auto=format&crop=faces&fit=crop&q=90")
Yr Athro Aled Clayton
Senior Lecturer
- Siarad Cymraeg
- Ar gael fel goruchwyliwr ôl-raddedig
Trosolwyg
I am part of the Tissue Microenvironment Group, School of Medicine, Cardiff University which is a collection of diverse and international researchers with a multidisciplinary approach to science.
My own specialist insterst involves studies of cell-derived vesicles. This is now a rapidly growing and exciting area of biology, but we have been able to establish some expertiese in this field since the early 2000's. The group has published well over 90 papers, most in relation to vesicles, and their roles in subverting immune responses, in controlling the cancer microenvironment and as potential disease biomarkers.
I am interested in understanding the mechanisms by which vesicle biogenesis and secretion is controlled, and the impact such vesicles have on the development and progression of cancer; particularly focussing on prostate cancer. Developing tools and assays to quantify nano-vesicles in biological fluid specimens is also an important aspect of the group's activities.
Although my main focus is in cancer biology, I collaborate broadly with researchers in Cardiff including those in the schools of dentistry, engineering and optometry, and interact with other Universities, on aspects including cardiovascular disease, neurodegenerative diseases and other conditions that impact our society in significant ways.
Cyhoeddiad
2024
- Zhang, X. et al. 2024. Surface acoustic wave‐enhanced multi‐view acoustofluidic rotation cytometry (MARC) for pre‐cytopathological screening. Advanced Science 11(39), article number: 2403574. (10.1002/advs.202403574)
- Bordanaba-Florit, G., Royo, F., Alboniga, O. E., Clayton, A., Falcon-Perez, J. M. and Webber, J. 2024. Integration of proteomic and metabolomic analysis reveal distinct metabolic alterations of prostate cancer-associated fibroblasts compared to normal fibroblasts from patient's stroma samples. BBA - Molecular Basis of Disease 1870(6), article number: 167229. (10.1016/j.bbadis.2024.167229)
2023
- Hyland, M., Mennan, C., Davies, R., Wilson, E., Tonge, D. P., Clayton, A. and Kehoe, O. 2023. Extracellular vesicles derived from umbilical cord mesenchymal stromal cells show enhanced anti-inflammatory properties via upregulation of miRNAs after pro-inflammatory priming. Stem Cell Reviews and Reports 19, pp. 2391-2406. (10.1007/s12015-023-10586-2)
- Dumcius, P. et al. 2023. Dual-wave acoustofluidic centrifuge for ultrafast concentration of nanoparticles and extracellular vesicles (small 35/2023). Small 19(35), article number: 2370274. (10.1002/smll.202370274)
- Dumcius, P. et al. 2023. Dual-Wave Acoustofluidic Centrifuge for Ultrafast Concentration of Nanoparticles and Extracellular Vesicles. Small 19(35), article number: 2300390. (10.1002/smll.202300390)
- Wang, H. et al. 2023. An enhanced tilted-angle acoustic tweezer for mechanical phenotyping of cancer cells. Analytica Chimica Acta 1255, article number: 341120. (10.1016/j.aca.2023.341120)
2022
- Owen, J. S., Clayton, A. and Pearson, H. B. 2022. Cancer-associated fibroblast heterogeneity, activation and function: implications for prostate cancer. Biomolecules 13(1), article number: 67. (10.3390/biom13010067)
- Knight, R. et al. 2022. Oral progenitor cell line-derived small extracellular vesicles as a treatment for preferential wound healing outcome. Stem Cells Translational Medicine 11(8), pp. 861-875. (10.1093/stcltm/szac037)
- van Niel, G., Carter, D. R. F., Clayton, A., Lambert, D. W., Raposo, G. and Vader, P. 2022. Challenges and directions in studying cell-cell communication by extracellular vesicles. Nature Reviews Molecular Cell Biology 23, pp. 369–382. (10.1038/s41580-022-00460-3)
- Brown, S. V., Dewitt, S., Clayton, A. and Waddington, R. J. 2022. Identifying the efficacy of extracellular vesicles in osteogenic differentiation: an EV-Lution in regenerative medicine. Frontiers in Dental Medicine 3, article number: 849724. (10.3389/fdmed.2022.849724)
2021
- Brown, H., Clayton, A. and Stephens, P. 2021. The role of bacterial extracellular vesicles in chronic wound infections: current knowledge and future challenges. Wound Repair and Regeneration 29(6), pp. 864-880. (10.1111/wrr.12949)
- Mikhaylov, R. et al. 2021. A reconfigurable and portable acoustofluidic system based on flexible printed circuit board for the manipulation of microspheres. Journal of Micromechanics and Microengineering 31(7), article number: 74003. (10.1088/1361-6439/ac0515)
- Erdbrügger, U. et al. 2021. Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles. Journal of Extracellular Vesicles 10(7), article number: e12093. (10.1002/jev2.12093)
- Lanning, B., Webber, J., Uysal-Ongane, P., Jiang, W. G., Clayton, A. and Dart, D. A. 2021. Prostate cancer cell extracellular vesicles increase mineralisation of bone osteoblast precursor cells in an in vitro model. Biology 10(4), article number: 318. (10.3390/biology10040318)
- Wu, F. et al. 2021. An enhanced tilted-angle acoustofluidic chip for cancer cell manipulation. IEEE Electron Device Letters 42(4), pp. 577-580. (10.1109/LED.2021.3062292)
- Evans, L., Milward, K., Attanoos, R., Clayton, A., Errington, R. and Tabi, Z. 2021. Macrophage plasticity and function in the lung tumour microenvironment revealed in 3D heterotypic spheroid and explant models. Biomedicines 9(3), article number: 302. (10.3390/biomedicines9030302)
2020
- Sun, C. et al. 2020. Gallium nitride: a versatile compound semiconductor as novel piezoelectric film for acoustic tweezer in manipulation of cancer cells. IEEE Transactions on Electron Devices 67(8), pp. 3355-3361. (10.1109/TED.2020.3002498)
- Mikhaylov, R. et al. 2020. Development and characterisation of acoustofluidic devices using detachable electrodes made from PCB. Lab on a Chip 20(10), pp. 1807-1814. (10.1039/C9LC01192G)
- Bliss, C. M. et al. 2020. Targeting antigen to the surface of EVs improves the in vivo immunogenicity of human and non-human adenoviral vaccines in mice. Molecular Therapy - Methods and Clinical Development 16, pp. 108-125. (10.1016/j.omtm.2019.12.003)
2019
- Clayton, A. et al. 2019. Considerations towards a roadmap for collection, handling and storage of blood extracellular vesicles. Journal of Extracellular Vesicles 8(1), article number: 1647027. (10.1080/20013078.2019.1647027)
- Brook, A. C. et al. 2019. Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis. Scientific Reports 9, article number: 10136. (10.1038/s41598-019-46585-y)
- Spaull, R., McPherson, B., Gialeli, A., Clayton, A., Uney, J., Heep, A. and Cordero-Llana, ?. 2019. Exosomes populate the cerebrospinal fluid of preterm infants with post-haemorrhagic hydrocephalus. International Journal of Developmental Neuroscience 73, pp. 59-65. (10.1016/j.ijdevneu.2019.01.004)
- Seed, R. I. et al. 2019. The putative tumour suppressor protein Latexin is secreted by prostate luminal cells and is downregulated in malignancy. Scientific Reports 9(1), article number: 5120. (10.1038/s41598-019-41379-8)
- Faruqu, F. N. et al. 2019. Membrane radiolabelling of exosomes for comparative biodistribution analysis in immunocompetent and immunodeficient mice – a novel and universal approach. Theranostics 9(6), pp. 1666-1682. (10.7150/thno.27891)
2018
- Théry, C. et al. 2018. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. Journal of Extracellular Vesicles 7(1), article number: 1535750. (10.1080/20013078.2018.1535750)
- Clayton, A. et al. 2018. Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017. Journal of Extracellular Vesicles 7(1), article number: 1473707. (10.1080/20013078.2018.1473707)
- Yeung, V. et al. 2018. Rab35-dependent extracellular nanovesicles are required for induction of tumour supporting stroma. Nanoscale 10(18), pp. 8547-8559. (10.1039/C8NR02417K)
- Slatter, D. A. et al. 2018. Enzymatically oxidized phospholipids restore thrombin generation in coagulation factor deficiencies. JCI Insight 3(6), article number: e98459. (10.1172/jci.insight.98459)
- Carter, D. R. F., Clayton, A., Devitt, A., Hunt, S. and Lambert, D. W. 2018. Extracellular vesicles in the tumour microenvironment. Philosophical Transactions B: Biological Sciences 373(1737), article number: 20160475. (10.1098/rstb.2016.0475)
2017
- Martinez, V. G., O'Neill, S., Salimu, J., Breslin, S., Clayton, A., Crown, J. and O'Driscoll, L. 2017. Resistance to HER2-targeted anti-cancer drugs is associated with immune evasion in cancer cells and their derived extracellular vesicles. OncoImmunology 6(12), article number: e1362530. (10.1080/2162402X.2017.1362530)
- Griffiths, S., Cormier, M., Clayton, A. and Doucette, A. 2017. Differential proteome analysis of extracellular vesicles from breast cancer cell lines by chaperone affinity enrichment. Proteomes 5(4), article number: 25. (10.3390/proteomes5040025)
- Roberts-Dalton, H. D. et al. 2017. Fluorescence labelling of extracellular vesicles using a novel thiol-based strategy for quantitative analysis of cellular delivery and intracellular traffic. Nanoscale 9(36), pp. 13693-13706. (10.1039/C7NR04128D)
- Welton, J. L., Loveless, S., Stone, T., Von Ruhland, C., Robertson, N. and Clayton, A. 2017. Cerebrospinal fluid extracellular vesicle enrichment for protein biomarker discovery in neurological disease; multiple sclerosis. Journal of Extracellular Vesicles 6(1), article number: 1369805. (10.1080/20013078.2017.1369805)
- Pathan, M. et al. 2017. A novel community driven software for functional enrichment analysis of extracellular vesicles data. Journal of Extracellular Vesicles 6, article number: 1321455. (10.1080/20013078.2017.1321455)
- Salimu, J., Webber, J., Gurney, M., Al-Taei, S., Clayton, A. and Tabi, Z. 2017. Dominant immunosuppression of dendritic cell function by prostate-cancer-derived exosomes. Journal of Extracellular Vesicles 6(1), article number: 1368823. (10.1080/20013078.2017.1368823)
2016
- Al-Taei, S., Salimu, J., Spary, L. K., Clayton, A., Lester, J. F. and Tabi, Z. 2016. Prostaglandin E2-mediated adenosinergic effects on CD14+ cells: self-amplifying immunosuppression in cancer. OncoImmunology 6(2), pp. 312-318., article number: e1268308. (10.1080/2162402X.2016.1268308)
- Welton, J. . L. et al. 2016. Proteomics analysis of vesicles isolated from plasma and urine of prostate cancer patients using a multiplex, aptamer-based protein array. Journal of Extracellular Vesicles 5, article number: 31209. (10.3402/jev.v5.31209)
- Emanueli, C. et al. 2016. Coronary artery-bypass-graft surgery increases the plasma concentration of exosomes carrying a cargo of cardiac microRNAs: an example of exosome trafficking out of the human heart with potential for cardiac biomarker discovery. PLoS ONE 11(4), article number: e0154274. (10.1371/journal.pone.0154274)
- Webber, J. P., Spary, L. K., Mason, M. D., Tabi, Z., Brewis, I. A. and Clayton, A. 2016. Prostate stromal cell proteomics analysis discriminates normal from tumour reactive stromal phenotypes. Oncotarget 7, pp. 20124-20139. (10.18632/oncotarget.7716)
- Clayton, A., Lawson, C., Gardiner, C., Harrison, P. and Carter, D. 2016. The 2nd United Kingdom Extracellular Vesicle Forum Meeting Abstracts. Journal of Extracellular Vesicles 5(1), article number: 30924. (10.3402/jev.v5.30924)
2015
- Connolly, K. D. et al. 2015. Characterisation of adipocyte-derived extracellular vesicles released pre- and post-adipogenesis. Journal of Extracellular Vesicles 4(s1), article number: 29159. (10.3402/jev.v4.29159)
- Beltrami, C. et al. 2015. Stabilization of urinary microRNAs by association with exosomes and argonaute 2 protein. Non-Coding RNA 1(2), pp. 151-166. (10.3390/ncrna1020151)
- Salimu, J., Spary, L. K., Al-Taei, S., Clayton, A., Mason, M. D., Staffurth, J. and Tabi, Z. 2015. Cross-Presentation of the Oncofetal Tumor Antigen 5T4 from Irradiated Prostate Cancer Cells—A Key Role for Heat-Shock Protein 70 and Receptor CD91. Cancer Immunology Research 3(6), pp. 678-688. (10.1158/2326-6066.CIR-14-0079)
- Webber, J. P., Yeung, V. and Clayton, A. 2015. Extracellular vesicles as modulators of the cancer microenvironment. Seminars in Cell and Developmental Biology 40, pp. 27-34. (10.1016/j.semcdb.2015.01.013)
- Welton, J. L., Webber, J. P., Botos, L., Jones, M. and Clayton, A. 2015. Ready-made chromatography columns for extracellular vesicle isolation from plasma. Journal of Extracellular Vesicles 4, article number: 27269. (10.3402/jev.v4.27269)
- Kim, D. et al. 2015. EVpedia: a community web portal for extracellular vesicles research. Bioinformatics 31(6), pp. 933-939. (10.1093/bioinformatics/btu741)
- Chowdhury, R., Webber, J. P., Gurney, M., Mason, M. D., Tabi, Z. and Clayton, A. 2015. Cancer exosomes trigger mesenchymal stem cell differentiation into pro-angiogenic and pro-invasive myofibroblasts. Oncotarget 6(2), pp. 715-731. (10.18632/oncotarget.2711)
- Webber, J. P. et al. 2015. Differentiation of tumour-promoting stromal myofibroblasts by cancer exosomes. Oncogene 34(3), pp. 290-302. (10.1038/onc.2013.560)
2014
- Willis, G. R. et al. 2014. Young women with polycystic ovary syndrome have raised levels of circulating annexin V-positive platelet microparticles. Human Reproduction 29(12), pp. 2756-2763. (10.1093/humrep/deu281)
- Galluzzi, L. et al. 2014. Classification of current anticancer immunotherapies. Oncotargets and Therapy 5(24), pp. 12472-12508. (10.18632/oncotarget.2998)
- Spary, L. K., Salimu, J., Webber, J. P., Clayton, A., Mason, M. d. and Tabi, Z. 2014. Tumor stroma-derived factors skew monocyte to dendritic cell differentiation toward a suppressive CD14+PD-L1+phenotype in prostate cancer. OncoImmunology 3(9), article number: e955331. (10.4161/21624011.2014.955331)
- Spary, L. K. et al. 2014. Enhancement of T cell responses as a result of synergy between lower doses of radiation and T cell stimulation. The Journal of Immunology 192(7), pp. 3101-3110. (10.4049/jimmunol.1302736)
- Webber, J. et al. 2014. Proteomics analysis of cancer exosomes using a novel modified aptamer-based array (SOMAscanTM) platform. Molecular and Cellular Proteomics 13(4), pp. 1050-1064. (10.1074/mcp.M113.032136)
2013
- Brennan, S. E., Nham, T., Clayton, A., McGowan, E. M., Cozzi, P. J. and Martiniello-Wilks, R. 2013. Prostate cancer exosomes offering novel circulating biomarkers for early cancer diagnosis and prognosis [Abstract]. The Journal of Gene Medicine 15(8-9), pp. 331-332. (10.1002/jgm.2740)
- Midgley, A. C., Rogers, M. J., Hallett, M. B., Clayton, A., Bowen, T., Phillips, A. O. and Steadman, R. 2013. Transforming growth factor-β1 (TGF-β1)-stimulated fibroblast to myofibroblast differentiation Is mediated by hyaluronan (HA)-facilitated epidermal growth factor receptor (EGFR) and CD44 co-localization in lipid rafts. Journal of Biological Chemistry 288(21), pp. 14824-14838. (10.1074/jbc.M113.451336)
- Webber, J. P. and Clayton, A. 2013. How pure are your vesicles?. Journal of Extracellular Vesicles 2, article number: 19861. (10.3402/jev.v2i0.19861)
2012
- Kalra, H. et al. 2012. Vesiclepedia: A compendium for extracellular vesicles with continuous community annotation. PLoS Biology 10(12), article number: e1001450. (10.1371/journal.pbio.1001450)
- Shah, K. M. et al. 2012. Induction of microRNA resistance and secretion in differentiating human endometrial stromal cells [Letter]. Journal of Molecular Cell Biology 5(1), pp. 67-70. (10.1093/jmcb/mjs058)
- Beltrami, C., Clayton, A., Phillips, A. O., Fraser, D. J. and Bowen, T. 2012. Analysis of urinary microRNAs in chronic kidney disease. Biochemical Society Transactions 40(4), pp. 875-879. (10.1042/BST20120090)
- Clayton, A. 2012. Cancer cells use exosomes as tools to manipulate immunity and the microenvironment. OncoImmunology 1(1), pp. 78-80. (10.4161/onci.1.1.17826)
2011
- Clayton, A., Al-Taei, S., Webber, J. P., Mason, M. D. and Tabi, Z. 2011. Cancer exosomes express CD39 and CD73, which suppress T cells through adenosine production. The Journal of Immunology 187(2), pp. 676-683. (10.4049/jimmunol.1003884)
2010
- Webber, J. P., Steadman, R., Mason, M. D., Tabi, Z. and Clayton, A. 2010. Cancer exosomes trigger fibroblast to myofibroblast differentiation. Cancer Research 70(23), pp. 9621-9630. (10.1158/0008-5472.CAN-10-1722)
- Tabi, Z., Spary, L. K., Coleman, S. L., Clayton, A., Mason, M. D. and Staffurth, J. N. 2010. Resistance of CD45RA- T cells to apoptosis and functional impairment, and activation of tumor-antigen specific T cells during radiation therapy of prostate cancer. The Journal of Immunology 185(2), pp. 1330-1339. (10.4049/jimmunol.1000488)
- Welton, J. L. et al. 2010. Proteomics analysis of bladder cancer exosomes. Molecular & Cellular Proteomics 9(6), pp. 1324-1338. (10.1074/mcp.M000063-MCP201)
2009
- Mitchell, P. J., Welton, J. L., Staffurth, J. N., Court, J., Mason, M. D., Tabi, Z. and Clayton, A. 2009. Can urinary exosomes act as treatment response markers in prostate cancer?. Journal Of Translational Medicine 7(4), pp. n/a. (10.1186/1479-5876-7-4)
- Navabi, H. et al. 2009. A clinical grade poly I:C-analogue (Ampligen®) promotes optimal DC maturation and Th1-type T cell responses of healthy donors and cancer patients in vitro. Vaccine 27(1), pp. 107-115. (10.1016/j.vaccine.2008.10.024)
- Clayton, A. and Mason, M. D. 2009. Exosomes in tumour immunity. Current Oncology -Toronto- 16(3), pp. 46-49.
2008
- Clayton, A., Mitchell, J. P., Court, J., Linnane, S., Mason, M. D. and Tabi, Z. 2008. Human tumor-derived exosomes down-modulate NKG2D expression. Journal of Immunology 180(11), pp. 7249-7258.
- Mitchell, J. P., Court, J., Mason, M. D., Tabi, Z. and Clayton, A. 2008. Increased exosome production from tumour cell cultures using the Integra CELLine Culture System. Journal of Immunological Methods 335(1-2), pp. 98-105. (10.1016/j.jim.2008.03.001)
2007
- Clayton, A., Mason, M. D., Mitchell, J. P. and Tabi, Z. 2007. Human tumour-derived exosomes selectively impair lymphocyte responses to Interleukin-2. Cancer Research 67, pp. 7458-7466. (10.1158/0008-5472.CAN-06-3456)
2006
- Théry, C., Amigorena, S., Raposo, G. and Clayton, A. 2006. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Current Protocols in Cell Biology, article number: Unit 322. (10.1002/0471143030.cb0322s30)
2005
- Clayton, A., Turkes, A., Mason, M. D. and Tabi, Z. 2005. Induction of heat shock proteins in B-cell exosomes. Journal of Cell Science 118(16), pp. 3631-3638. (10.1242/jcs.02494)
- Coleman, S. L., Clayton, A., Mason, M. D., Jasani, B., Adams, M. and Tabi, Z. 2005. Recovery of CD8+ T-cell function during systemic chemotherapy in advanced ovarian cancer. Cancer Research 65(15), pp. 7000-6. (10.1158/0008-5472.CAN-04-3792)
- Coleman, S. L., Clayton, A., Mason, M. D., Jasani, B., Adams, M. and Tabi, Z. 2005. Recovery of CD8+ T-cell function during systemic chemotherapy in advanced ovarian cancer. Cancer Research 65(15), pp. 7000-7006. (10.1158/0008-5472.CAN-04-3792)
- Adams, M. et al. 2005. The rationale for combined chemo/immunotherapy using a Toll-like receptor 3 (TLR3) agonist and tumour-derived exosomes in advanced ovarian cancer. Vaccine 23(17-18), pp. 2374-2378. (10.1016/j.vaccine.2005.01.014)
- Navabi, H. et al. 2005. Preparation of human ovarian cancer ascites-derived exosomes for a clinical trial. Blood Cells, Molecules, and Diseases 35(2), pp. 149-152. (10.1016/j.bcmd.2005.06.008)
- Clayton, A. and Tabi, Z. 2005. Exosomes and the MICA-NKG2D system in cancer. Blood Cells, Molecules, and Diseases 34(3), pp. 206-213. (10.1016/j.bcmd.2005.03.003)
2004
- Clayton, A., Turkes, A., Dewitt, S., Steadman, R., Mason, M. D. and Hallett, M. B. 2004. Adhesion and signaling by B cell-derived exosomes: the role of integrins. The FASEB Journal 18, pp. 977-979. (10.1096/fj.03-1094fje)
2003
- Thomas, G. J., Clayton, A., Thomas, J., Davies, M. and Steadman, R. 2003. Structural and functional changes in heparan sulfate proteoglycan expression associated with the myofibroblastic phenotype. America Journal of Pathology 162(3), pp. 977-989.
- Clayton, A., Harris, C. L., Morgan, B. P. and Mason, M. D. 2003. Antigen-presenting cell exosomes are protected from complement-mediated lysis by expression of CD55 and CD59. European Journal of Immunology 33(2), pp. 522-31. (10.1002/immu.200310028)
- Blaber, R., Stylianou, E., Clayton, A. and Steadman, R. 2003. Selective regulation of ICAM-1 and RANTES gene expression after ICAM-1 ligation on human renal fibroblasts. Journal of the American Society of Nephrology 14(1), pp. 116-127. (10.1097/01.ASN.0000040595.35207.62)
2002
- Savage, P. et al. 2002. Anti-viral cytotoxic T cells inhibit the growth of cancer cells with antibody targeted hla class I/peptide complexes in scid mice. International Journal of Cancer 98(4), pp. 561-566. (10.1002/ijc.10219)
- Savage, P. et al. 2002. Induction of viral and tumour specific CTL responses using antibody targeted HLA class I peptide complexes. British Journal of Cancer 86(8), pp. 1336-1342. (10.1038/sj.bjc.6600223)
2001
- Clayton, A., Thomas, J., Thomas, G. J., Davies, M. and Steadman, R. 2001. Cell surface heparan sulfate proteoglycans control the response of renal interstitial fibroblasts to fibroblast growth factor-2. Kidney International 59(6), pp. 2084-2094. (10.1046/j.1523-1755.2001.00723.x)
- Clayton, A. et al. 2001. Analysis of antigen presenting cell derived exosomes, based on immuno-magnetic isolation and flow cytometry. Journal of Immunological Methods 247(1-2), pp. 163-174. (10.1016/S0022-1759(00)00321-5)
1999
- Clayton, A. and Steadman, R. 1999. ICAM-1 interactions in the renal interstitium: a novel activator of fibroblasts during nephritis. Histology and Histopathology 14(3), pp. 861-870.
- Clayton, A., Thomas, J., Thomas, G., Davies, M. and Steadman, R. 1999. Fibroblasts from the cortical interstitium express cell surface heparan sulphate proteoglycans [Abstract]. Kidney International 55(6), pp. 2588-2588. (10.1046/j.1523-1755.2002.t01-1-00456.x)
- Evans, R. E., Clayton, A., Martin, J., Williams, J. D., Davies, M. and Steadman, R. 1999. Neutrophil binding stimulates the synthesis, release and activation of MMP 3 and MMP 2 from endothelial cells in vitro [Abstract]. Kidney International 55(5), pp. 2105-2105. (10.1046/j.1523-1755.1999.00404.x)
1998
- Clayton, A., Evans, R. A., Pettit, E., Hallett, M., Williams, J. D. and Steadman, R. 1998. Cellular activation through the ligation of intercellular adhesion molecule-1. Journal of Cell Science 111(4), pp. 443-453.
1997
- Evans, R. A., Clayton, A., Martin, J., Williams, J. D., Davies, M. and Steadman, R. 1997. Neutrophil binding stimulates the synthesis, release and activation of MMP 2 from endothelial cells in vitro. Journal of the American Society of Nephrology 8, pp. A2390-A2390.
- Clayton, A., Williams, J. D. and Steadman, R. 1997. The ligation of ICAM-1 on renal cortical fibroblasts initiates the de novo expression of ICAM-1 and VCAM-1. Journal of the American Society of Nephrology 8, pp. A2385-A2385.
- Clayton, A., Williams, J. D. and Steadman, R. 1997. Ligation of ICAM-1 on the surface of renal cortical fibroblasts stimulates de novo expression of ICAM-1 and VCAM-1 [Abstract]. Kidney International 52(1), pp. 262-263. (10.1038/ki.1997.329)
- Clayton, A., Steadman, R. and Williams, J. D. 1997. Cells isolated from the human cortical interstitium resemble myofibroblasts and bind neutrophils in an ICAM-1--dependent manner. Journal of the American Society of Nephrology 8(4), pp. 604-615.
1996
- Steadman, R., Clayton, A., Pettit, J., Hallett, B. and Williams, J. D. 1996. Leukocytes trigger an influx of calcium in renal interstitial fibroblasts through ligation ICAM-1. Journal of the American Society of Nephrology 7(9), pp. A2594-A2594.
1995
- Clayton, A., Steadman, R. and Williams, J. D. 1995. Human renal cortical fibroblasts (RCF) bind leukocytes in-vitro through a CD18-dependent and VLA(4)-dependent interaction. Journal of the American Society of Nephrology 6(3), pp. 894-894.
Erthyglau
- Zhang, X. et al. 2024. Surface acoustic wave‐enhanced multi‐view acoustofluidic rotation cytometry (MARC) for pre‐cytopathological screening. Advanced Science 11(39), article number: 2403574. (10.1002/advs.202403574)
- Bordanaba-Florit, G., Royo, F., Alboniga, O. E., Clayton, A., Falcon-Perez, J. M. and Webber, J. 2024. Integration of proteomic and metabolomic analysis reveal distinct metabolic alterations of prostate cancer-associated fibroblasts compared to normal fibroblasts from patient's stroma samples. BBA - Molecular Basis of Disease 1870(6), article number: 167229. (10.1016/j.bbadis.2024.167229)
- Hyland, M., Mennan, C., Davies, R., Wilson, E., Tonge, D. P., Clayton, A. and Kehoe, O. 2023. Extracellular vesicles derived from umbilical cord mesenchymal stromal cells show enhanced anti-inflammatory properties via upregulation of miRNAs after pro-inflammatory priming. Stem Cell Reviews and Reports 19, pp. 2391-2406. (10.1007/s12015-023-10586-2)
- Dumcius, P. et al. 2023. Dual-wave acoustofluidic centrifuge for ultrafast concentration of nanoparticles and extracellular vesicles (small 35/2023). Small 19(35), article number: 2370274. (10.1002/smll.202370274)
- Dumcius, P. et al. 2023. Dual-Wave Acoustofluidic Centrifuge for Ultrafast Concentration of Nanoparticles and Extracellular Vesicles. Small 19(35), article number: 2300390. (10.1002/smll.202300390)
- Wang, H. et al. 2023. An enhanced tilted-angle acoustic tweezer for mechanical phenotyping of cancer cells. Analytica Chimica Acta 1255, article number: 341120. (10.1016/j.aca.2023.341120)
- Owen, J. S., Clayton, A. and Pearson, H. B. 2022. Cancer-associated fibroblast heterogeneity, activation and function: implications for prostate cancer. Biomolecules 13(1), article number: 67. (10.3390/biom13010067)
- Knight, R. et al. 2022. Oral progenitor cell line-derived small extracellular vesicles as a treatment for preferential wound healing outcome. Stem Cells Translational Medicine 11(8), pp. 861-875. (10.1093/stcltm/szac037)
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- Welton, J. L. et al. 2010. Proteomics analysis of bladder cancer exosomes. Molecular & Cellular Proteomics 9(6), pp. 1324-1338. (10.1074/mcp.M000063-MCP201)
- Mitchell, P. J., Welton, J. L., Staffurth, J. N., Court, J., Mason, M. D., Tabi, Z. and Clayton, A. 2009. Can urinary exosomes act as treatment response markers in prostate cancer?. Journal Of Translational Medicine 7(4), pp. n/a. (10.1186/1479-5876-7-4)
- Clayton, A. and Mason, M. D. 2009. Exosomes in tumour immunity. Current Oncology -Toronto- 16(3), pp. 46-49.
- Clayton, A., Mitchell, J. P., Court, J., Linnane, S., Mason, M. D. and Tabi, Z. 2008. Human tumor-derived exosomes down-modulate NKG2D expression. Journal of Immunology 180(11), pp. 7249-7258.
- Mitchell, J. P., Court, J., Mason, M. D., Tabi, Z. and Clayton, A. 2008. Increased exosome production from tumour cell cultures using the Integra CELLine Culture System. Journal of Immunological Methods 335(1-2), pp. 98-105. (10.1016/j.jim.2008.03.001)
- Clayton, A., Mason, M. D., Mitchell, J. P. and Tabi, Z. 2007. Human tumour-derived exosomes selectively impair lymphocyte responses to Interleukin-2. Cancer Research 67, pp. 7458-7466. (10.1158/0008-5472.CAN-06-3456)
- Théry, C., Amigorena, S., Raposo, G. and Clayton, A. 2006. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Current Protocols in Cell Biology, article number: Unit 322. (10.1002/0471143030.cb0322s30)
- Clayton, A., Turkes, A., Mason, M. D. and Tabi, Z. 2005. Induction of heat shock proteins in B-cell exosomes. Journal of Cell Science 118(16), pp. 3631-3638. (10.1242/jcs.02494)
- Coleman, S. L., Clayton, A., Mason, M. D., Jasani, B., Adams, M. and Tabi, Z. 2005. Recovery of CD8+ T-cell function during systemic chemotherapy in advanced ovarian cancer. Cancer Research 65(15), pp. 7000-7006. (10.1158/0008-5472.CAN-04-3792)
Ymchwil
We are interested in understanding how cells produce small fat-bubbles (vesicles), and why they seem to do this so extensively. Our studies focus on the role of such bubbles in changing the immediate surroundings of the cancer tissue. it seems these bubbles (exosomes) can interfere with the correct function of immune cells, they can encourage formation of blood vessels, and can hijack other neighbouring cells to behave abnormally- all of these effects assist the growth and ultimate spread of the tumour.
We can also detect exosomes in the urine and blood of cancer patients, and we are working hard on developing new methods to isolate exosomes from biological fluids, and to use these as means of discovering new indicators of disease. We have examined the complex array of protein molecules on the vesicles, and are beginning to also look at RNA-molecules, which hide inside the bubble.
Together, these studies will provide much needed information about how the cancer cells can dominate and take-over their microenvironments. Learning how to block these effects is likely to be very useful therapeutically. Pperhaps we can also use the presence of exosomes in urine/blood as future tests to support diagnoses and monitoring of cancer.
Funding sources
- Cancer Research Wales, Programme grant:
- Movember: The Movember Revolutionary Team Award
- Prostate Cancer UK: British Heart Foundation:
- British Lung Foundation:
- CU & ReNeuron:
- Tenovus Cancer Care:
- Welsh Assembly Government NRN Sêr Cymru Award:
Addysgu
Intercalated BSc and Pharmacology degrees:
- Develop and mark lab-based reseach projects.
- Mark student research presentations
College of Biomedical Life Science
- MSc in Cancer Biology (2016)
Postgraduate Students
Primary Supervisor:
- Mr Vincent Yeung (2013- end 2016), co-supervisor Dr Jason Webber
- Mr Alex Cocks (2015-2018), co-supervisors Prof. Arwyn T Jones (PHRMY) and Dr Peter Watson (BIOSCI)
Co-Supervisor
- Mr Rhodri Thomas (Vesicle RNA in Alzheimer's Disease), supervised with Prof Julie Williams
- Miss Donna Mathews (Vesicles in Cardio Vascular Disease), supervised with Dr Aled Rees & Prof Philip James
- Mr Robert Knight (Vesicles in wound healing), supervised with Prof Philip Stephens and Dr Julie Albon
- Mr Ben Lanning (micro RNA and vesicles in cancer), supervised with Dr Alwyn Dart & Dr Bronwen Evans
additional contributions through collaborative interactions with PhD candidates
- Miss Hope Roberts Dalton, (PHRMY), supervised by Prof Arwyn T Jones.
Bywgraffiad
Career overview
- Snr Lecturer (08/2013-present), Division of Cancer & Genetics, Cardiff University
- Lecturer; (08/2004 to 07/2013), School of Medicine, Cardiff University
- Research Fellow (01/1999 to 07/2004) School of Medicine, Cardiff University
- Research Fellow, (10/1996 to 12/1998) School of Medicine, Cardiff University
Education & qualifications
- 1997: PhD (Medicine), Institute of Nephrology, University of Wales College of Medicine.
- 1998: Diploma in Biomedical methods, University of Wales College of Medicine.
- 1993: BSc (Hons) Biochemistry, University of Wales College Cardiff
Anrhydeddau a dyfarniadau
- Visiting Professor for the Finish Government inFlames Programme at the university of Turku https://inflames.utu.fi
Aelodaethau proffesiynol
- Founding Member of the International Society for Extracellular vesicles (ISEV)
- Founding Member of the United kingdom Extracellular Vesicles Forum (UKEV Forum)
- Member of Cardiff Institute for Tissue Engineering and Repair (CITER).
Pwyllgorau ac adolygu
External Committees
- 2015-2017: NCRI Clinical Studies Group - Molecular Biomarkers Advisory Group
- 2015- United Kingdom Extracellular vesicle Forum, annual conference organiser and co-organiser 2014, 2016 ongoing.
- 2016- International Society for Extracellular vesicles, Annual Conference Local Organising Committee, Rotterdam
- 2016-2017- Organising Committee, Royal Society Discussion Meeting, EV in the Cancer Microenvironment, London 2017.
PhD Examiner
- 2017. External Examiner, PhD St Andrews
- 2016. External Examiner, PhD Leicester University
- 2015. Second Opponent, PhD Oslo, Norway
- 2014. External Examiner, PhD Oxford Brookes
- 2013. Internal Examiner, PhD, Cardiff
Editor
- 2012-Ongoing: Associate Editorial Board member - Journal of Extracellular vesicles
- 2013-Ongoing: Editorial Board, Journal of Circulating Biomarkers.
Meysydd goruchwyliaeth
The team are very interested in persuing studies related to
- nano-vesicle measurements/analytics
- vesicle-mediated intercellular comunication including drug-delivery applications
- vesicles in basic cancer biology that may include immunological functions, and roles in modulating cancer associated fibroblasts
- the utility of vesicles as cancer markers (particularly in prostate cancer).
PhD Supervisor:
- Miss. Mariama Mbengue, co-supervised with Dr Jason Webber & Prof. Rachel Errington
Undertaking an investigation of intracellular factors controlling secretion of vesicle subsets, and the consequence of attenuating some of these on vesicle-mediated intercellular communication.
- Miss. Jasmine Owen, co-supervised with Dr Helen Pearson (ECSCRI, BIOSCI)
Undertaking an investigation of stromal cells in Prostate Cancer
- Mr. Keran Hodson, co-supervised with Dr Toby Phesse (ECSCRI, BIOSCI)
Investigating contributions of vesicles to Wnt-signalling in cancer.
Contact Details
ClaytonA@caerdydd.ac.uk
+44 29225 10589
Adeilad Tenovus, Ystafell GF16, Ysbyty Athrofaol Cymru, Parc y Mynydd Bychan, Caerdydd, CF14 4XN
+44 29225 10589
Adeilad Tenovus, Ystafell GF16, Ysbyty Athrofaol Cymru, Parc y Mynydd Bychan, Caerdydd, CF14 4XN
