![Tomasz Jurkowski](https://profiles-cardiff.imgix.net/attachments/1087?w=200&h=200&auto=format&crop=faces&fit=crop&q=90")
Dr Tomasz Jurkowski
Reader
Ysgol y Biowyddorau
- Ar gael fel goruchwyliwr ôl-raddedig
Trosolwyg
Research overview
I want to understand how a cell’s differentiation state is established and how it is maintained over the whole lifetime of an organism. Because the cell’s differentiation state is encoded in the epigenetic blueprint, the stability of the cellular differentiation state depends on the stability of the underlying epigenetic profile. However, as the enzymes maintaining the epigenetic states are responsive to various parameters, epigenetic signatures are also dynamic in nature, thus providing transcriptional plasticity in response to environmental stimuli and intrinsic and extrinsic cellular stress. My group employs an interdisciplinary, modular approach to dissect the chromatin networks responsible for establishing and maintaining the epigenetic signals.
Current research within the group splits across three interdisciplinary themes:
- Understanding the biochemistry and specificity of epigenetic machinery (with a focus on DNA methyltransferases and demethylases)
- Synthetic epigenetics - developing tools and applying them to study and control cell epigenetic system
- Single-cell multi-omics - developing novel technology to study epigenetic and transcriptional profiles of single cells
Cyhoeddiad
2024
- Weiser, R., Ronchetti, K., Tame, J., Hoehn, S., Jurkowski, T. P., Mahenthiralingam, E. and Forton, J. T. 2024. The fungal diversity in the lungs of children with cystic fibrosis captured by sputum-induction and bronchoalveolar lavage. Journal of Cystic Fibrosis (10.1016/j.jcf.2024.07.011)
- Gilbert, G. et al. 2024. Drosophila TET acts with PRC1 to activate gene expression independently of its catalytic activity. Science Advances 10(18), article number: eadn5861. (10.1126/sciadv.adn5861)
2023
- Boulet, M. et al. 2023. Adenine methylation is very scarce in the Drosophila genome and not erased by the ten-eleven translocation dioxygenase. eLife 12, article number: RP91655. (10.7554/elife.91655)
- -Eshalomi, C., Aliyev, E., Hoehn, S., Jurkowski, T. P. and Swann, K. 2023. Sperm induce a secondary increase in ATP levels in mouse eggs that is independent of Ca2+ oscillations. Biochemical Journal 480(24), pp. 2023-2035. (10.1042/bcj20230065)
- Nan, X. et al. 2023. VarLOCK: sequencing-independent, rapid detection of SARS-CoV-2 variants of concern for point-of-care testing, qPCR pipelines and national wastewater surveillance. Scientific Reports 13(1), article number: 20832. (10.1038/s41598-023-47289-0)
- Kucharski, R., Ellis, N., Jurkowski, T. P., Hurd, P. J. and Maleszka, R. 2023. The PWWP domain and the evolution of unique DNA methylation toolkits in Hymenoptera. iScience 26(11), article number: 108193. (10.1016/j.isci.2023.108193)
- Schwartz, U. et al. 2023. High-resolution transcriptomic and epigenetic profiling identifies novel regulators of COPD. The EMBO Journal 42, article number: e111272. (10.15252/embj.2022111272)
- Dordevic, M. et al. 2023. EpiCRISPR targeted methylation of Arx gene initiates transient switch of mouse pancreatic alpha to insulin-producing cells. Frontiers in Endocrinology 14, article number: 1134478. (10.3389/fendo.2023.1134478)
2022
- Weiser, R. et al. 2022. The lung microbiota in children with cystic fibrosis captured by induced sputum sampling. Journal of Cystic Fibrosis 21(6), pp. 1006-1012. (10.1016/j.jcf.2022.01.006)
- Ravichandran, M. et al. 2022. Pronounced sequence specificity of the TET enzyme catalytic domain guides its cellular function. Science Advances 8(36) (10.1126/sciadv.abm2427)
- Rasa, S. M. M. et al. 2022. Inflammaging is driven by upregulation of innate immune receptors and systemic interferon signaling and is ameliorated by dietary restriction. Cell Reports 39(13), article number: 111017. (10.1016/j.celrep.2022.111017)
- Tolic, A. et al. 2022. TET-mediated DNA hydroxymethylation is negatively influenced by the PARP-dependent PARylation. Epigenetics and Chromatin 15(11) (10.1186/s13072-022-00445-8)
- Nan, X. et al. 2022. VarLOCK - sequencing independent, rapid detection of SARS-CoV-2 variants of concern for point-of-care testing, qPCR pipelines and national wastewater surveillance. [Online]. medRxix: (10.1101/2022.01.06.21268555) Available at: https://www.medrxiv.org/content/10.1101/2022.01.06.21268555v1
2021
- Liu, C. et al. 2021. Epigenetic modulation of radiation-induced diacylglycerol kinase alpha expression prevents pro-fibrotic fibroblast response. Cancers 13(10), article number: 2455. (10.3390/cancers13102455)
2020
- Tomkuviene, M. et al. 2020. Enzymatic hydroxylation and excision of extended 5-methylcytosine analogues. Journal of Molecular Biology 432(23), pp. 6157-6167. (10.1016/j.jmb.2020.10.011)
2019
- Daum, R., Brauchle, E. M., Carvajal Berrio, D. A., Jurkowski, T. P. and Schenke-Layland, K. 2019. Non-invasive detection of DNA methylation states in carcinoma and pluripotent stem cells using Raman microspectroscopy and imaging. Scientific Reports 9(1), article number: 7014. (10.1038/s41598-019-43520-z)
- Tolic, A. et al. 2019. Enrichment of Cxcl12 promoter with TET2: A possible link between promoter demethylation and enhanced gene expression in the absence of PARP-1. Archives of Biological Sciences 71(3), pp. 455. (10.2298/ABS190404027T)
- Hipp, K. et al. 2019. Different forms of African cassava mosaic virus capsid protein within plants and virions. Virology 529, pp. 81-90. (10.1016/j.virol.2019.01.018)
- Oberacker, P. et al. 2019. Bio-On-Magnetic-Beads (BOMB): Open platform for high-throughput nucleic acid extraction and manipulation. PLoS Biology 17(1), article number: e3000107. (10.1371/journal.pbio.3000107)
- Vidaković, M., Tolić, A., Grdović, N., Ravichandran, M. and Jurkowski, T. P. 2019. PARylation, DNA (de)methylation, and diabetes. In: Handbook of Nutrition, Diet, and Epigenetics. Switzerland: Springer, Cham, pp. 1857-1876., (10.1007/978-3-319-55530-0_55)
2018
- Schwenger, A., Jurkowski, T. P. and Richert, C. 2018. Capturing and stabilizing folded proteins in lattices formed with branched oligonucleotide hybrids. ChemBioChem 19(14), pp. 1523-1530. (10.1002/cbic.201800145)
- Ravichandran, M., Jurkowska, R. and Jurkowski, T. 2018. Target specificity of mammalian DNA methylation and demethylation machinery. Organic and Biomolecular Chemistry(9), pp. 1419-1435. (10.1039/C7OB02574B)
2017
- Jurkowska, R. Z. et al. 2017. H3K14ac is linked to methylation of H3K9 by the triple Tudor domain of SETDB1. Nature Communications 8, article number: 2057. (10.1038/s41467-017-02259-9)
- Saunderson, E. A. et al. 2017. Hit-and-run epigenetic editing prevents senescence entry in primary breast cells from healthy donors. Nature Communications 8(1), article number: 1450. (10.1038/s41467-017-01078-2)
- Kaiser, S., Jurkowski, T. P., Kellner, S., Schneider, D., Jeltsch, A. and Helm, M. 2017. The RNA methyltransferase Dnmt2 methylates DNA in the structural context of a tRNA. RNA Biology 14(9), pp. 1241—1251. (10.1080/15476286.2016.1236170)
- Song, J. et al. 2017. Targeted epigenetic editing of SPDEF reduces mucus production in lung epithelial cells. AJP - Lung Cellular and Molecular Physiology 312(3), pp. L334—L347. (10.1152/ajplung.00059.2016)
- Stepper, P. et al. 2017. Efficient targeted DNA methylation with chimeric dCas9-Dnmt3a-Dnmt3L methyltransferase. Nucleic Acids Research 45(4), pp. 1703-1713. (10.1093/nar/gkw1112)
2016
- Hore, T. A. et al. 2016. Retinol and ascorbate drive erasure of epigenetic memory and enhance reprogramming to naïve pluripotency by complementary mechanisms. Proceedings of the National Academy of Sciences 113(43), pp. 12202—12207. (10.1073/pnas.1608679113)
- Jeltsch, A. et al. 2016. Mechanism and biological role of Dnmt2 in Nucleic Acid Methylation. RNA Biology 14(9), pp. 1108—1123. (10.1080/15476286.2016.1191737)
2015
- Patalano, S. et al. 2015. Molecular signatures of plastic phenotypes in two eusocial insect species with simple societies. Proceedings of the National Academy of Sciences 112(45), pp. 13970—13975. (10.1073/pnas.1515937112)
- Maier, J. A. H., Albu, R. F., Jurkowski, T. P. and Jeltsch, A. 2015. Investigation of the C-terminal domain of the bacterial DNA-(adenine N6)-methyltransferase CcrM. Biochimie 119, pp. 60—67. (10.1016/j.biochi.2015.10.011)
- Delatte, B. et al. 2015. Genome-wide hydroxymethylcytosine pattern changes in response to oxidative stress. Scientific Reports 5, article number: 12714. (10.1038/srep12714)
- Shanmugam, R., Fierer, J., Kaiser, S., Helm, M., Jurkowski, T. P. and Jeltsch, A. 2015. Cytosine methylation of tRNA-Asp by DNMT2 has a role in translation of proteins containing poly-Asp sequences. Cell discovery 1, article number: 15010. (10.1038/celldisc.2015.10)
- Subinya, M., Steudle, A. K., Jurkowski, T. P. and Stubenrauch, C. 2015. Conformation and activity of lipase B from Candida antarctica in bicontinuous microemulsions. Colloids and Surfaces B: Biointerfaces 131, pp. 108-114. (10.1016/j.colsurfb.2015.04.041)
- Elhardt, W., Shanmugam, R., Jurkowski, T. P. and Jeltsch, A. 2015. Somatic cancer mutations in the DNMT2 tRNA methyltransferase alter its catalytic properties. Biochimie 112, pp. 66—72. (10.1016/j.biochi.2015.02.022)
- Jurkowski, T. P., Ravichandran, M. and Stepper, P. 2015. Synthetic epigenetics-towards intelligent control of epigenetic states and cell identity. Clinical Epigenetics 7, pp. 18. (10.1186/s13148-015-0044-x)
2014
- Deplus, R. et al. 2014. Regulation of DNA methylation patterns by CK2-mediated phosphorylation of Dnmt3a. Cell Reports 8(3), pp. 743—753. (10.1016/j.celrep.2014.06.048)
- Shanmugam, R. et al. 2014. The Dnmt2 RNA methyltransferase homolog of Geobacter sulfurreducens specifically methylates tRNA-Glu. Nucleic Acids Research 42(10), pp. 6487—6496. (10.1093/nar/gku256)
2013
- Müller, S. et al. 2013. Target recognition, RNA methylation activity and transcriptional regulation of the Dictyostelium discoideum Dnmt2-homologue (DnmA). Nucleic Acids Research 41(18), pp. 8615—8627. (10.1093/nar/gkt634)
2012
- Siddique, A. N. et al. 2012. Targeted methylation and gene silencing of VEGF-A in human cells by using a designed Dnmt3a-Dnmt3L single-chain fusion protein with increased DNA methylation activity. Journal of Molecular Biology 425(3), pp. 479—491. (10.1016/j.jmb.2012.11.038)
- Becker, M., Müller, S., Nellen, W., Jurkowski, T. P., Jeltsch, A. and Ehrenhofer-Murray, A. E. 2012. Pmt1, a Dnmt2 homolog in Schizosaccharomyces pombe, mediates tRNA methylation in response to nutrient signaling. Nucleic Acids Research 40(22), pp. 11648—11658. (10.1093/nar/gks956)
- Jurkowski, T. P., Shanmugam, R., Helm, M. and Jeltsch, A. 2012. Mapping the tRNA binding site on the surface of human DNMT2 methyltransferase. Biochemistry 51(22), pp. 4438—4444. (10.1021/bi3002659)
- Albu, R. F., Zacharias, M., Jurkowski, T. P. and Jeltsch, A. 2012. DNA interaction of the CcrM DNA methyltransferase: a mutational and modeling study. ChemBioChem 13(9), pp. 1304-1311. (10.1002/cbic.201200082)
- Albu, R. F., Jurkowski, T. P. and Jeltsch, A. 2012. The Caulobacter crescentus DNA-(adenine-N6)-methyltransferase CcrM methylates DNA in a distributive manner. Nucleic Acids Research 40(4), pp. 1708—1716. (10.1093/nar/gkr768)
2011
- Jurkowski, T. P. and Jeltsch, A. 2011. On the evolutionary origin of eukaryotic DNA methyltransferases and Dnmt2. PLoS ONE 6(11), pp. e28104. (10.1371/journal.pone.0028104)
- Jurkowska, R. Z., Jurkowski, T. P. and Jeltsch, A. 2011. Structure and function of mammalian DNA methyltransferases. ChemBioChem 12(2), pp. 206—222. (10.1002/cbic.201000195)
- Jurkowski, T. P. and Jeltsch, A. 2011. Burning off DNA methylation: new evidence for oxygen-dependent DNA demethylation. ChemBioChem 12(17), pp. 2543—2545. (10.1002/cbic.201100549)
- Jurkowska, R. Z., Siddique, A. N., Jurkowski, T. P. and Jeltsch, A. 2011. Approaches to enzyme and substrate design of the murine Dnmt3a DNA methyltransferase. ChemBioChem 12(10), pp. 1589—1594. (10.1002/cbic.201000673)
- Siddique, A. N., Jurkowska, R. Z., Jurkowski, T. P. and Jeltsch, A. 2011. Auto-methylation of the mouse DNA-(cytosine C5)-methyltransferase Dnmt3a at its active site cysteine residue. FEBS Journal 278(12), pp. 2055—2063. (10.1111/j.1742-4658.2011.08121.x)
2009
- Zhang, Y. et al. 2009. DNA methylation analysis of chromosome 21 gene promoters at single base pair and single allele resolution. PLoS Genetics 5(3), article number: e1000438. (10.1371/journal.pgen.1000438)
2008
- Jurkowski, T. P., Meusburger, M., Phalke, S., Helm, M., Nellen, W., Reuter, G. and Jeltsch, A. 2008. Human DNMT2 methylates tRNA(Asp) molecules using a DNA methyltransferase-like catalytic mechanism. RNA 14(8), pp. 1663—1670. (10.1261/rna.970408)
- Dhayalan, A., Jurkowski, T. P., Laser, H., Reinhardt, R., Jia, D., Cheng, X. and Jeltsch, A. 2008. Mapping of protein-protein interaction sites by the 'absence of interference' approach. Journal of Molecular Biology 376(4), pp. 1091—1099. (10.1016/j.jmb.2007.12.032)
- Rohde, C., Zhang, Y., Jurkowski, T. P., Stamerjohanns, H., Reinhardt, R. and Jeltsch, A. 2008. Bisulfite sequencing Data Presentation and Compilation (BDPC) web server--a useful tool for DNA methylation analysis. Nucleic Acids Research 36(5), pp. e34. (10.1093/nar/gkn083)
2007
- Jurkowski, T. P., Anspach, N., Kulishova, L., Nellen, W. and Jeltsch, A. 2007. The M.EcoRV DNA-(adenine N6)-methyltransferase uses DNA bending for recognition of an expanded EcoDam recognition site. Journal of Biological Chemistry 282(51), pp. 36942—36952. (10.1074/jbc.m706933200)
- Jeltsch, A., Jurkowska, R. Z., Jurkowski, T. P., Liebert, K., Rathert, P. and Schlickenrieder, M. 2007. Application of DNA methyltransferases in targeted DNA methylation. Applied Microbiology and Biotechnology 75(6), pp. 1233—1240. (10.1007/s00253-007-0966-0)
2006
- Gowher, H., Loutchanwoot, P., Vorobjeva, O., Handa, V., Jurkowska, R. Z., Jurkowski, T. P. and Jeltsch, A. 2006. Mutational analysis of the catalytic domain of the murine Dnmt3a DNA-(cytosine C5)-methyltransferase. Journal of Molecular Biology 357(3), pp. 928—941. (10.1016/j.jmb.2006.01.035)
Articles
- Weiser, R., Ronchetti, K., Tame, J., Hoehn, S., Jurkowski, T. P., Mahenthiralingam, E. and Forton, J. T. 2024. The fungal diversity in the lungs of children with cystic fibrosis captured by sputum-induction and bronchoalveolar lavage. Journal of Cystic Fibrosis (10.1016/j.jcf.2024.07.011)
- Gilbert, G. et al. 2024. Drosophila TET acts with PRC1 to activate gene expression independently of its catalytic activity. Science Advances 10(18), article number: eadn5861. (10.1126/sciadv.adn5861)
- Boulet, M. et al. 2023. Adenine methylation is very scarce in the Drosophila genome and not erased by the ten-eleven translocation dioxygenase. eLife 12, article number: RP91655. (10.7554/elife.91655)
- -Eshalomi, C., Aliyev, E., Hoehn, S., Jurkowski, T. P. and Swann, K. 2023. Sperm induce a secondary increase in ATP levels in mouse eggs that is independent of Ca2+ oscillations. Biochemical Journal 480(24), pp. 2023-2035. (10.1042/bcj20230065)
- Nan, X. et al. 2023. VarLOCK: sequencing-independent, rapid detection of SARS-CoV-2 variants of concern for point-of-care testing, qPCR pipelines and national wastewater surveillance. Scientific Reports 13(1), article number: 20832. (10.1038/s41598-023-47289-0)
- Kucharski, R., Ellis, N., Jurkowski, T. P., Hurd, P. J. and Maleszka, R. 2023. The PWWP domain and the evolution of unique DNA methylation toolkits in Hymenoptera. iScience 26(11), article number: 108193. (10.1016/j.isci.2023.108193)
- Schwartz, U. et al. 2023. High-resolution transcriptomic and epigenetic profiling identifies novel regulators of COPD. The EMBO Journal 42, article number: e111272. (10.15252/embj.2022111272)
- Dordevic, M. et al. 2023. EpiCRISPR targeted methylation of Arx gene initiates transient switch of mouse pancreatic alpha to insulin-producing cells. Frontiers in Endocrinology 14, article number: 1134478. (10.3389/fendo.2023.1134478)
- Weiser, R. et al. 2022. The lung microbiota in children with cystic fibrosis captured by induced sputum sampling. Journal of Cystic Fibrosis 21(6), pp. 1006-1012. (10.1016/j.jcf.2022.01.006)
- Ravichandran, M. et al. 2022. Pronounced sequence specificity of the TET enzyme catalytic domain guides its cellular function. Science Advances 8(36) (10.1126/sciadv.abm2427)
- Rasa, S. M. M. et al. 2022. Inflammaging is driven by upregulation of innate immune receptors and systemic interferon signaling and is ameliorated by dietary restriction. Cell Reports 39(13), article number: 111017. (10.1016/j.celrep.2022.111017)
- Tolic, A. et al. 2022. TET-mediated DNA hydroxymethylation is negatively influenced by the PARP-dependent PARylation. Epigenetics and Chromatin 15(11) (10.1186/s13072-022-00445-8)
- Liu, C. et al. 2021. Epigenetic modulation of radiation-induced diacylglycerol kinase alpha expression prevents pro-fibrotic fibroblast response. Cancers 13(10), article number: 2455. (10.3390/cancers13102455)
- Tomkuviene, M. et al. 2020. Enzymatic hydroxylation and excision of extended 5-methylcytosine analogues. Journal of Molecular Biology 432(23), pp. 6157-6167. (10.1016/j.jmb.2020.10.011)
- Daum, R., Brauchle, E. M., Carvajal Berrio, D. A., Jurkowski, T. P. and Schenke-Layland, K. 2019. Non-invasive detection of DNA methylation states in carcinoma and pluripotent stem cells using Raman microspectroscopy and imaging. Scientific Reports 9(1), article number: 7014. (10.1038/s41598-019-43520-z)
- Tolic, A. et al. 2019. Enrichment of Cxcl12 promoter with TET2: A possible link between promoter demethylation and enhanced gene expression in the absence of PARP-1. Archives of Biological Sciences 71(3), pp. 455. (10.2298/ABS190404027T)
- Hipp, K. et al. 2019. Different forms of African cassava mosaic virus capsid protein within plants and virions. Virology 529, pp. 81-90. (10.1016/j.virol.2019.01.018)
- Oberacker, P. et al. 2019. Bio-On-Magnetic-Beads (BOMB): Open platform for high-throughput nucleic acid extraction and manipulation. PLoS Biology 17(1), article number: e3000107. (10.1371/journal.pbio.3000107)
- Schwenger, A., Jurkowski, T. P. and Richert, C. 2018. Capturing and stabilizing folded proteins in lattices formed with branched oligonucleotide hybrids. ChemBioChem 19(14), pp. 1523-1530. (10.1002/cbic.201800145)
- Ravichandran, M., Jurkowska, R. and Jurkowski, T. 2018. Target specificity of mammalian DNA methylation and demethylation machinery. Organic and Biomolecular Chemistry(9), pp. 1419-1435. (10.1039/C7OB02574B)
- Jurkowska, R. Z. et al. 2017. H3K14ac is linked to methylation of H3K9 by the triple Tudor domain of SETDB1. Nature Communications 8, article number: 2057. (10.1038/s41467-017-02259-9)
- Saunderson, E. A. et al. 2017. Hit-and-run epigenetic editing prevents senescence entry in primary breast cells from healthy donors. Nature Communications 8(1), article number: 1450. (10.1038/s41467-017-01078-2)
- Kaiser, S., Jurkowski, T. P., Kellner, S., Schneider, D., Jeltsch, A. and Helm, M. 2017. The RNA methyltransferase Dnmt2 methylates DNA in the structural context of a tRNA. RNA Biology 14(9), pp. 1241—1251. (10.1080/15476286.2016.1236170)
- Song, J. et al. 2017. Targeted epigenetic editing of SPDEF reduces mucus production in lung epithelial cells. AJP - Lung Cellular and Molecular Physiology 312(3), pp. L334—L347. (10.1152/ajplung.00059.2016)
- Stepper, P. et al. 2017. Efficient targeted DNA methylation with chimeric dCas9-Dnmt3a-Dnmt3L methyltransferase. Nucleic Acids Research 45(4), pp. 1703-1713. (10.1093/nar/gkw1112)
- Hore, T. A. et al. 2016. Retinol and ascorbate drive erasure of epigenetic memory and enhance reprogramming to naïve pluripotency by complementary mechanisms. Proceedings of the National Academy of Sciences 113(43), pp. 12202—12207. (10.1073/pnas.1608679113)
- Jeltsch, A. et al. 2016. Mechanism and biological role of Dnmt2 in Nucleic Acid Methylation. RNA Biology 14(9), pp. 1108—1123. (10.1080/15476286.2016.1191737)
- Patalano, S. et al. 2015. Molecular signatures of plastic phenotypes in two eusocial insect species with simple societies. Proceedings of the National Academy of Sciences 112(45), pp. 13970—13975. (10.1073/pnas.1515937112)
- Maier, J. A. H., Albu, R. F., Jurkowski, T. P. and Jeltsch, A. 2015. Investigation of the C-terminal domain of the bacterial DNA-(adenine N6)-methyltransferase CcrM. Biochimie 119, pp. 60—67. (10.1016/j.biochi.2015.10.011)
- Delatte, B. et al. 2015. Genome-wide hydroxymethylcytosine pattern changes in response to oxidative stress. Scientific Reports 5, article number: 12714. (10.1038/srep12714)
- Shanmugam, R., Fierer, J., Kaiser, S., Helm, M., Jurkowski, T. P. and Jeltsch, A. 2015. Cytosine methylation of tRNA-Asp by DNMT2 has a role in translation of proteins containing poly-Asp sequences. Cell discovery 1, article number: 15010. (10.1038/celldisc.2015.10)
- Subinya, M., Steudle, A. K., Jurkowski, T. P. and Stubenrauch, C. 2015. Conformation and activity of lipase B from Candida antarctica in bicontinuous microemulsions. Colloids and Surfaces B: Biointerfaces 131, pp. 108-114. (10.1016/j.colsurfb.2015.04.041)
- Elhardt, W., Shanmugam, R., Jurkowski, T. P. and Jeltsch, A. 2015. Somatic cancer mutations in the DNMT2 tRNA methyltransferase alter its catalytic properties. Biochimie 112, pp. 66—72. (10.1016/j.biochi.2015.02.022)
- Jurkowski, T. P., Ravichandran, M. and Stepper, P. 2015. Synthetic epigenetics-towards intelligent control of epigenetic states and cell identity. Clinical Epigenetics 7, pp. 18. (10.1186/s13148-015-0044-x)
- Deplus, R. et al. 2014. Regulation of DNA methylation patterns by CK2-mediated phosphorylation of Dnmt3a. Cell Reports 8(3), pp. 743—753. (10.1016/j.celrep.2014.06.048)
- Shanmugam, R. et al. 2014. The Dnmt2 RNA methyltransferase homolog of Geobacter sulfurreducens specifically methylates tRNA-Glu. Nucleic Acids Research 42(10), pp. 6487—6496. (10.1093/nar/gku256)
- Müller, S. et al. 2013. Target recognition, RNA methylation activity and transcriptional regulation of the Dictyostelium discoideum Dnmt2-homologue (DnmA). Nucleic Acids Research 41(18), pp. 8615—8627. (10.1093/nar/gkt634)
- Siddique, A. N. et al. 2012. Targeted methylation and gene silencing of VEGF-A in human cells by using a designed Dnmt3a-Dnmt3L single-chain fusion protein with increased DNA methylation activity. Journal of Molecular Biology 425(3), pp. 479—491. (10.1016/j.jmb.2012.11.038)
- Becker, M., Müller, S., Nellen, W., Jurkowski, T. P., Jeltsch, A. and Ehrenhofer-Murray, A. E. 2012. Pmt1, a Dnmt2 homolog in Schizosaccharomyces pombe, mediates tRNA methylation in response to nutrient signaling. Nucleic Acids Research 40(22), pp. 11648—11658. (10.1093/nar/gks956)
- Jurkowski, T. P., Shanmugam, R., Helm, M. and Jeltsch, A. 2012. Mapping the tRNA binding site on the surface of human DNMT2 methyltransferase. Biochemistry 51(22), pp. 4438—4444. (10.1021/bi3002659)
- Albu, R. F., Zacharias, M., Jurkowski, T. P. and Jeltsch, A. 2012. DNA interaction of the CcrM DNA methyltransferase: a mutational and modeling study. ChemBioChem 13(9), pp. 1304-1311. (10.1002/cbic.201200082)
- Albu, R. F., Jurkowski, T. P. and Jeltsch, A. 2012. The Caulobacter crescentus DNA-(adenine-N6)-methyltransferase CcrM methylates DNA in a distributive manner. Nucleic Acids Research 40(4), pp. 1708—1716. (10.1093/nar/gkr768)
- Jurkowski, T. P. and Jeltsch, A. 2011. On the evolutionary origin of eukaryotic DNA methyltransferases and Dnmt2. PLoS ONE 6(11), pp. e28104. (10.1371/journal.pone.0028104)
- Jurkowska, R. Z., Jurkowski, T. P. and Jeltsch, A. 2011. Structure and function of mammalian DNA methyltransferases. ChemBioChem 12(2), pp. 206—222. (10.1002/cbic.201000195)
- Jurkowski, T. P. and Jeltsch, A. 2011. Burning off DNA methylation: new evidence for oxygen-dependent DNA demethylation. ChemBioChem 12(17), pp. 2543—2545. (10.1002/cbic.201100549)
- Jurkowska, R. Z., Siddique, A. N., Jurkowski, T. P. and Jeltsch, A. 2011. Approaches to enzyme and substrate design of the murine Dnmt3a DNA methyltransferase. ChemBioChem 12(10), pp. 1589—1594. (10.1002/cbic.201000673)
- Siddique, A. N., Jurkowska, R. Z., Jurkowski, T. P. and Jeltsch, A. 2011. Auto-methylation of the mouse DNA-(cytosine C5)-methyltransferase Dnmt3a at its active site cysteine residue. FEBS Journal 278(12), pp. 2055—2063. (10.1111/j.1742-4658.2011.08121.x)
- Zhang, Y. et al. 2009. DNA methylation analysis of chromosome 21 gene promoters at single base pair and single allele resolution. PLoS Genetics 5(3), article number: e1000438. (10.1371/journal.pgen.1000438)
- Jurkowski, T. P., Meusburger, M., Phalke, S., Helm, M., Nellen, W., Reuter, G. and Jeltsch, A. 2008. Human DNMT2 methylates tRNA(Asp) molecules using a DNA methyltransferase-like catalytic mechanism. RNA 14(8), pp. 1663—1670. (10.1261/rna.970408)
- Dhayalan, A., Jurkowski, T. P., Laser, H., Reinhardt, R., Jia, D., Cheng, X. and Jeltsch, A. 2008. Mapping of protein-protein interaction sites by the 'absence of interference' approach. Journal of Molecular Biology 376(4), pp. 1091—1099. (10.1016/j.jmb.2007.12.032)
- Rohde, C., Zhang, Y., Jurkowski, T. P., Stamerjohanns, H., Reinhardt, R. and Jeltsch, A. 2008. Bisulfite sequencing Data Presentation and Compilation (BDPC) web server--a useful tool for DNA methylation analysis. Nucleic Acids Research 36(5), pp. e34. (10.1093/nar/gkn083)
- Jurkowski, T. P., Anspach, N., Kulishova, L., Nellen, W. and Jeltsch, A. 2007. The M.EcoRV DNA-(adenine N6)-methyltransferase uses DNA bending for recognition of an expanded EcoDam recognition site. Journal of Biological Chemistry 282(51), pp. 36942—36952. (10.1074/jbc.m706933200)
- Jeltsch, A., Jurkowska, R. Z., Jurkowski, T. P., Liebert, K., Rathert, P. and Schlickenrieder, M. 2007. Application of DNA methyltransferases in targeted DNA methylation. Applied Microbiology and Biotechnology 75(6), pp. 1233—1240. (10.1007/s00253-007-0966-0)
- Gowher, H., Loutchanwoot, P., Vorobjeva, O., Handa, V., Jurkowska, R. Z., Jurkowski, T. P. and Jeltsch, A. 2006. Mutational analysis of the catalytic domain of the murine Dnmt3a DNA-(cytosine C5)-methyltransferase. Journal of Molecular Biology 357(3), pp. 928—941. (10.1016/j.jmb.2006.01.035)
Book sections
- Vidaković, M., Tolić, A., Grdović, N., Ravichandran, M. and Jurkowski, T. P. 2019. PARylation, DNA (de)methylation, and diabetes. In: Handbook of Nutrition, Diet, and Epigenetics. Switzerland: Springer, Cham, pp. 1857-1876., (10.1007/978-3-319-55530-0_55)
Websites
- Nan, X. et al. 2022. VarLOCK - sequencing independent, rapid detection of SARS-CoV-2 variants of concern for point-of-care testing, qPCR pipelines and national wastewater surveillance. [Online]. medRxix: (10.1101/2022.01.06.21268555) Available at: https://www.medrxiv.org/content/10.1101/2022.01.06.21268555v1
Bywgraffiad
Tomasz Jurkowski studied Molecular Biology at Warsaw University (PL) (1999-2004). He did his MSc thesis in the laboratory of Dr Janusz Bujnicki (IIMCB, PL) and Dr Monika Radlinska (Warsaw University, PL). Afterwards, he joined the group of Dr Albert Jeltsch at Jacobs University Bremen for his doctoral training, which he completed in 2008. After 4 years of post-doctoral training at Jacobs University in 2012, he joined the Faculty of Chemistry at the University of Stuttgart as a Junior Professor in Biochemistry and Molecular Epigenetics. In 2019 he joined Cardiff University as Senior Lecturer in Mammalian Systems.
Academic positions
- 2019 – present: Senior Lecturer for Mammalian Systems, Cardiff University
- 2012 – 2019: Junior Professor for Molecular Epigenetics and Biochemistry, Stuttgart University (Germany)
- 2009 – 2012: 50% Postdoctoral Researcher, 50% Teaching Lecturer, Jacobs University Bremen (Germany)
Meysydd goruchwyliaeth
I am interested in supervising students in:
- Molecular epigenetics: Biochemistry of epigenetic machinery
- Synthetic epigenetics: Developing and applying innovative tools to study and manipulate epigenetic systems in the cells
- Biochemistry of nucleic acid modifying enzymes: from polymerases to oxidases
- Single-cell multiomics: New methods to study single cells using cutting edge new omics tools
- High-throughput assays and automation
Contact Details
JurkowskiT@caerdydd.ac.uk
+44 29208 76291
Adeilad Syr Martin Evans, Rhodfa'r Amgueddfa, Caerdydd, CF10 3AX
+44 29208 76291
Adeilad Syr Martin Evans, Rhodfa'r Amgueddfa, Caerdydd, CF10 3AX
