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Yr Athro Emyr Lloyd-Evans
(e/fe)
DPhil (Oxon)
- Siarad Cymraeg
- Ar gael fel goruchwyliwr ôl-raddedig
Timau a rolau for Emyr Lloyd-Evans
Dirprwy Gyfarwyddwr, Sefydliad Darganfod Meddyginiaethau
Ysgol y Biowyddorau
Trosolwyg
Trosolwg ymchwil
Mae gan fy grŵp ddiddordeb mewn nodi a thrin y mecanweithiau sy'n arwain at farwolaeth celloedd yn yr anhwylderau storio lysosomal, sef yr achos mwyaf cyffredin o glefyd niwroddirywiol plentyndod. Mae gennym ddiddordeb hefyd mewn pennu'r tebygrwydd rhwng clefydau lysosomaidd a chlefydau niwroddirywiol cysylltiedig heneiddio, gan gynnwys Alzheimer, Parkinson's a Huntington's, lle credwn fod posibiliadau i drin mecanweithiau clefydau sylfaenol tebyg. Mae gennym ddiddordeb arbennig mewn swyddogaeth signalau Ca2+ lysosomal, endocytosis, awtophagy a phrotein lysosomal (ensymau, sianeli a chludwyr). Ein nod yw nodi targedau allweddol sy'n berthnasol i glefydau y gellir eu holi gan brofion cadarn er mwyn sgrinio a nodi clefydau newydd sy'n addasu cyfansoddion offer moleciwlau bach. Yna defnyddir bioleg strwythurol a chemeg feddyginiaethol i gynhyrchu a darparu meddyginiaethau moleciwl bach gwell sy'n cael eu profi mewn modelau clefyd cellog newydd. Mae gan fy ngrŵp hanes o fynd o fainc i wely, ac o gydweithio â phartneriaid diwydiant a sefydliadau cleifion i ddarparu therapïau newydd ar gyfer y clefydau dinistriol hyn.
Cyhoeddiad
2026
- Ferreira, N. G. et al., 2026. Non-linear transcriptional responses suggest mechanisms of tributyltin toxicity in Triops longicaudatus. Environmental Pollution 407 128735. (10.1016/j.envpol.2026.128735)
- Best, H. et al. 2026. The long and short of it: Distinct natural crystal packing strategies of Cry toxins from Bacillus thuringiensis. Structure 34 (5), pp.704-717.e3. (10.1016/j.str.2026.01.014)
2025
- Kirkham, E. et al., 2025. Lysosomal pH measurements via dual-imaging microscopy. In: Hughes, S. M. and Basak, I. eds. Lysosomes: Methods and Protocols. Vol. 2976, Methods in Molecular Biology New York, New York, USA: , pp.11-24. (10.1007/978-1-0716-4844-5_2)
- Reeks, J. et al., 2025. High throughput cryo-EM provides structural understanding for modulators of the lysosomal ion channel TRPML1. Structure 33 (8), pp.1374-1385. (10.1016/j.str.2025.05.014)
- Best, H. L. et al. 2025. Niemann-Pick C-like endo-lysosomal dysfunction in DHDDS patient cells, a congenital disorder of glycosylation, can be treated with miglustat. International Journal of Molecular Sciences 26 (4) 1471. (10.3390/ijms26041471)
2024
- Cubitt, J. et al. 2024. Beware of N-Benzoyloxybenzamides. Molecules 29 (21) 5143. (10.3390/molecules29215143)
2023
- Williamson, L. J. et al. 2023. Structure of the Lysinibacillus sphaericus Tpp49Aa1 pesticidal protein elucidated from natural crystals using MHz-SFX. Proceedings of the National Academy of Sciences 120 (49) e2203241120. (10.1073/pnas.2203241120)
- Best, H. L. et al. 2023. The role of glycoconjugates as receptors for insecticidal proteins. FEMS Microbiology Reviews 47 (4)(10.1093/femsre/fuad026)
- Somogyi, A. et al., 2023. The synthetic TRPML1 agonist ML-SA1 rescues Alzheimer-related alterations of the endosomal-autophagic-lysosomal system. Journal of Cell Science 136 (6) jcs259875. (10.1242/jcs.259875)
- Fenn, G. D. et al. 2023. Development of a novel anthraquinone-derived fluorescent lysosomal probe. Molecular Genetics and Metabolism 138 (2) 107097. (10.1016/j.ymgme.2022.107097)
- Best, H. et al. 2023. The Batten disease associated protein CLN3 is required for the efflux of lysosomal K. Presented at: 19th Annual WORLDSymposium™ 2023 Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.47-47. (10.1016/j.ymgme.2022.107030)
- Best, H. L. et al. 2023. Disease phenotypes present in CLN7 patient fibroblasts are improved following treatment to reduce glycosphingolipid synthesis.. Presented at: 19th Annual WORLDSymposium™ 2023 Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.17. (10.1016/j.ymgme.2022.107029)
- Cook, S. R. et al. 2023. Investigating lysosomal and cellular phenotypes of the lysosomal disorder cystinosis. Presented at: 19th Annual WORLDSymposium™ 2023 Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.28-28. (10.1016/j.ymgme.2022.107061)
- Cook, S. R. and Lloyd-Evans, E. 2023. Comparing the lysosomal biotoxicity of iron oxide nanoparticles for improved lysosomal purification. Presented at: 19th Annual WORLDSymposiumTM Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.28-29. (10.1016/j.ymgme.2022.107062)
- Fenn, G. D. , Lloyd-Evans, E. and Waller-Evans, H. 2023. Investigation of cannabidiol as a potential therapeutic in Niemann-Pick disease. Presented at: 19th Annual WORLDSymposium™ 2023 Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.42-42. (10.1016/j.ymgme.2022.107098)
- Honeybun, L. , Waller-Evans, H. and Lloyd-Evans, E. 2023. Unbiased phenotypic drug screen for CLN3 disease. Presented at: 19th Annual WORLDSymposium™ 2023 Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.167-167. (10.1016/j.ymgme.2022.107150)
- Lloyd-Evans, E. et al. 2023. Glycosphingolipid reduction with miglustat as a therapeutic strategy for CLN3 and other neuronal ceroid lipofuscinoses.. Presented at: 19th Annual WORLDSymposium™ 2023 Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.84. (10.1016/j.ymgme.2022.107212)
- Lloyd-Evans, E. and Evans, C. 2023. Deciphering the various mechanisms of action of miglustat in the lysosomal disorders.. Presented at: 19th Annual WORLDSymposiumTM Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.84. (10.1016/j.ymgme.2022.107213)
- Waller-Evans, H. et al. 2023. Filipin composition and imaging modality dramatically affect cellular cholesterol visualisation with implications for assessing cholesterol levels and localisation across the lysosomal disorders. Presented at: 19th Annual WORLDSymposium™ 2023 Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.133. (10.1016/j.ymgme.2022.107352)
- Waller-Evans, H. et al. 2023. Measuring ion flux via lysosomal channels: Primary and secondary assays for drug discovery across the lysosomal disorders. Presented at: 19th Annual WORLDSymposium™ 2023 Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.133-134. (10.1016/j.ymgme.2022.107353)
2022
- Best, H. L. et al. 2022. The crystal structure of Bacillus thuringiensis Tpp80Aa1 and its interaction with galactose-containing glycolipids. Toxins 14 (12) 863. (10.3390/toxins14120863)
2021
- Maguire, E. et al. 2021. PIP2 depletion and altered endocytosis caused by expression of Alzheimer's disease-protective variant PLCγ2 R522. EMBO Journal 40 (17) e105603. (10.15252/embj.2020105603)
2020
- Cook, S. R. et al. 2020. Visualisation of cholesterol and ganglioside GM1 in zebrafish models of Niemann-Pick type C disease and Smith-Lemli-Opitz syndrome using light sheet microscopy. Histochemistry and Cell Biology 154 , pp.565-578. (10.1007/s00418-020-01925-2)
- Badell Grau, R. A. et al. 2020. Investigating the prevalence of reactive online searching in the COVID-19 pandemic. Journal of Medical Internet Research 22 (10) e19791. (10.2196/19791)
- Harwood, J. L. and Lloyd-Evans, E. eds. 2020. Essays in biochemistry volume 64, issue 3: lipid mediators. Portland Press.
- Lloyd-Evans, E. and Waller-Evans, H. 2020. Biosynthesis and signalling functions of central and peripheral nervous system neurosteroids in health and disease. Essays in Biochemistry 64 (3), pp.591-606. (10.1042/EBC20200043)
- Cook, S. R. et al. 2020. Detrimental effect of zwitterionic buffers on lysosomal homeostasis in cell lines and iPSC-derived neurons. AMRC Open Research 2 21. (10.12688/amrcopenres.12903.1)
- Day, A. H. et al. 2020. Targeted cell imaging properties of a deep red luminescent iridium(III) complex conjugated with a c-Myc signal peptide. Chemical Science 11 (6), pp.1599-1606. (10.1039/C9SC05568A)
2019
- Carreira, A. et al., 2019. Mammalian sphingoid bases: Biophysical, physiological and pathological properties. Progress in Lipid Research 75 100988. (10.1016/j.plipres.2019.100988)
- Balogi, Z. et al., 2019. Hsp70 interactions with membrane lipids regulate cellular functions in health and disease. Progress in Lipid Research 74 , pp.18-30. (10.1016/j.plipres.2019.01.004)
2018
- Morimoto, M. et al., 2018. Bi-allelic CCDC47 variants cause a disorder characterized by woolly hair, liver dysfunction, dysmorphic features, and global developmental delay. American Journal of Human Genetics 103 (5), pp.794-807. (10.1016/j.ajhg.2018.09.014)
- Lange, J. et al., 2018. Compromised astrocyte function and survival negatively impact neurons in infantile neuronal ceroid lipofuscinosis. Acta Neuropathologica Communications 6 74. (10.1186/s40478-018-0575-4)
2017
- Fineran, P. et al., 2017. Pathogenic mycobacteria achieve cellular persistence by inhibiting the Niemann-Pick Type C disease cellular pathway. Wellcome Open Research 1 18. (10.12688/wellcomeopenres.10036.2)
2016
- Lloyd-Evans, E. and Haslett, L. 2016. The lysosomal storage disease continuum with ageing-related neurodegenerative disease. Ageing Research Reviews 32 , pp.104-121. (10.1016/j.arr.2016.07.005)
- Lloyd-Evans, E. 2016. On the move, lysosomal CAX drives Ca2+ transport and motility. Journal of cell biology 212 (7), pp.755-757. (10.1083/jcb.201603037)
2015
- Oswald, M. C. W. et al., 2015. Identification of dietary alanine toxicity and trafficking dysfunction in a Drosophila model of hereditary sensory and autonomic neuropathy type 1. Human Molecular Genetics 24 (24), pp.6899-6909. (10.1093/hmg/ddv390)
- Lee, J. et al., 2015. Presenilin 1 maintains Lysosomal Ca2+ homeostasis via TRPML1 by regulating vATPase-mediated lysosome acidification. Cell Reports 12 (9), pp.1430-1444. (10.1016/j.celrep.2015.07.050)
- Chandrachud, U. et al., 2015. Unbiased cell-based screening in a neuronal cell model of Batten Disease highlights an interaction between Ca2+Homeostasis, autophagy, and CLN3 protein function. Journal of Biological Chemistry 290 (23), pp.14361-14380.. (10.1074/jbc.M114.621706)
- Waller-Evans, H. and Lloyd-Evans, E. 2015. Regulation of TRPML1 function. Biochemical Society Transactions 43 (3), pp.442-446. (10.1042/BST20140311)
- Walker, M. W. and Lloyd-Evans, E. 2015. A rapid method for the preparation of ultrapure, functional lysosomes using functionalized superparamagnetic iron oxide nanoparticles. Methods in Cell Biology 126 , pp.21-43. (10.1016/bs.mcb.2014.10.019)
2014
- Platt, F. M. et al., 2014. Disorders of cholesterol metabolism and their unanticipated convergent mechanisms of disease. Annual Review of Genomics and Human Genetics 15 (1), pp.173-194. (10.1146/annurev-genom-091212-153412)
2011
- Morgan, A. J. et al. 2011. Molecular mechanisms of endolysosomal Ca2+ signalling in health and disease. Biochemical Journal 439 (3), pp.349-374. (10.1042/BJ20110949)
- Lloyd-Evans, E. and Platt, F. M. 2011. Lysosomal Ca2+ homeostasis: Role in pathogenesis of lysosomal storage diseases. Cell Calcium 50 (2), pp.200-205. (10.1016/j.ceca.2011.03.010)
2010
- Lloyd-Evans, E. and Platt, F. M. 2010. Lipids on trial: The search for the offending metabolite in Niemann-Pick type C disease. Traffic 11 (4), pp.419-428. (10.1111/j.1600-0854.2010.01032.x)
- Lloyd-Evans, E. et al. 2010. Endolysosomal calcium regulation and disease.. Biochemical Society Transactions 38 (6), pp.1458-1464. (10.1042/BST0381458)
- Ruas, M. et al., 2010. Purified TPC isoforms form NAADP receptors with distinct roles for Ca2+ signaling and endolysosomal trafficking. Current Biology 20 (8), pp.703-709. (10.1016/j.cub.2010.02.049)
2008
- Elliot-Smith, E. et al., 2008. Beneficial effects of substrate reduction therapy in a mouse model of GM1 gangliosidosis. Molecular Genetics and Metabolism 94 (2), pp.204-211. (10.1016/j.ymgme.2008.02.005)
- Lloyd-Evans, E. et al. 2008. Niemann-Pick disease type C1 is a sphingosine storage disease that causes deregulation of lysosomal calcium. Nature Medicine 14 (11), pp.1247-1255. (10.1038/nm.1876)
2005
- Pelled, D. et al., 2005. Enhanced calcium release in the acute neuronopathic form of Gaucher disease. Neurobiology of Disease 18 (1), pp.83-88. (10.1016/j.nbd.2004.09.004)
2004
- Lachmann, R. H. et al., 2004. Treatment with miglustat reverses the lipid-trafficking defect in Niemann–Pick disease type C. Neurobiology of Disease 16 (3), pp.654-658. (10.1016/j.nbd.2004.05.002)
- Vruchte, D. T. et al., 2004. Accumulation of glycosphingolipids in Niemann-Pick C disease disrupts endosomal transport. Journal of Biological Chemistry 279 (25), pp.26167-26175. (10.1074/jbc.M311591200)
2003
- Lloyd-Evans, E. et al. 2003. Glucosylceramide and glucosylsphingosine modulate calcium mobilization from brain microsomes via different mechanisms. Journal of Biological Chemistry 278 (26), pp.23594-23599. (10.1074/jbc.M300212200)
- Lloyd-Evans, E. et al. 2003. Lyso-glycosphingolipids mobilize calcium from brain microsomes via multiple mechanisms. Biochemical Journal 375 (3), pp.561-565. (10.1042/BJ20030613)
- Pelled, D. et al., 2003. Inhibition of calcium uptake via the sarco/endoplasmic reticulum Ca2+-ATPase in a mouse model of Sandhoff disease and prevention by treatment with N-Butyldeoxynojirimycin. Journal of Biological Chemistry 278 (32), pp.29496-29501. (10.1074/jbc.M302964200)
Adrannau llyfrau
- Kirkham, E. et al., 2025. Lysosomal pH measurements via dual-imaging microscopy. In: Hughes, S. M. and Basak, I. eds. Lysosomes: Methods and Protocols. Vol. 2976, Methods in Molecular Biology New York, New York, USA: , pp.11-24. (10.1007/978-1-0716-4844-5_2)
Cynadleddau
- Best, H. et al. 2023. The Batten disease associated protein CLN3 is required for the efflux of lysosomal K. Presented at: 19th Annual WORLDSymposium™ 2023 Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.47-47. (10.1016/j.ymgme.2022.107030)
- Best, H. L. et al. 2023. Disease phenotypes present in CLN7 patient fibroblasts are improved following treatment to reduce glycosphingolipid synthesis.. Presented at: 19th Annual WORLDSymposium™ 2023 Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.17. (10.1016/j.ymgme.2022.107029)
- Cook, S. R. et al. 2023. Investigating lysosomal and cellular phenotypes of the lysosomal disorder cystinosis. Presented at: 19th Annual WORLDSymposium™ 2023 Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.28-28. (10.1016/j.ymgme.2022.107061)
- Cook, S. R. and Lloyd-Evans, E. 2023. Comparing the lysosomal biotoxicity of iron oxide nanoparticles for improved lysosomal purification. Presented at: 19th Annual WORLDSymposiumTM Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.28-29. (10.1016/j.ymgme.2022.107062)
- Fenn, G. D. , Lloyd-Evans, E. and Waller-Evans, H. 2023. Investigation of cannabidiol as a potential therapeutic in Niemann-Pick disease. Presented at: 19th Annual WORLDSymposium™ 2023 Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.42-42. (10.1016/j.ymgme.2022.107098)
- Honeybun, L. , Waller-Evans, H. and Lloyd-Evans, E. 2023. Unbiased phenotypic drug screen for CLN3 disease. Presented at: 19th Annual WORLDSymposium™ 2023 Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.167-167. (10.1016/j.ymgme.2022.107150)
- Lloyd-Evans, E. et al. 2023. Glycosphingolipid reduction with miglustat as a therapeutic strategy for CLN3 and other neuronal ceroid lipofuscinoses.. Presented at: 19th Annual WORLDSymposium™ 2023 Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.84. (10.1016/j.ymgme.2022.107212)
- Lloyd-Evans, E. and Evans, C. 2023. Deciphering the various mechanisms of action of miglustat in the lysosomal disorders.. Presented at: 19th Annual WORLDSymposiumTM Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.84. (10.1016/j.ymgme.2022.107213)
- Waller-Evans, H. et al. 2023. Filipin composition and imaging modality dramatically affect cellular cholesterol visualisation with implications for assessing cholesterol levels and localisation across the lysosomal disorders. Presented at: 19th Annual WORLDSymposium™ 2023 Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.133. (10.1016/j.ymgme.2022.107352)
- Waller-Evans, H. et al. 2023. Measuring ion flux via lysosomal channels: Primary and secondary assays for drug discovery across the lysosomal disorders. Presented at: 19th Annual WORLDSymposium™ 2023 Orlando, Florida 21-26 February 2023. Vol. 138.Vol. 2. Elsevier. , pp.133-134. (10.1016/j.ymgme.2022.107353)
Erthyglau
- Ferreira, N. G. et al., 2026. Non-linear transcriptional responses suggest mechanisms of tributyltin toxicity in Triops longicaudatus. Environmental Pollution 407 128735. (10.1016/j.envpol.2026.128735)
- Best, H. et al. 2026. The long and short of it: Distinct natural crystal packing strategies of Cry toxins from Bacillus thuringiensis. Structure 34 (5), pp.704-717.e3. (10.1016/j.str.2026.01.014)
- Reeks, J. et al., 2025. High throughput cryo-EM provides structural understanding for modulators of the lysosomal ion channel TRPML1. Structure 33 (8), pp.1374-1385. (10.1016/j.str.2025.05.014)
- Best, H. L. et al. 2025. Niemann-Pick C-like endo-lysosomal dysfunction in DHDDS patient cells, a congenital disorder of glycosylation, can be treated with miglustat. International Journal of Molecular Sciences 26 (4) 1471. (10.3390/ijms26041471)
- Cubitt, J. et al. 2024. Beware of N-Benzoyloxybenzamides. Molecules 29 (21) 5143. (10.3390/molecules29215143)
- Williamson, L. J. et al. 2023. Structure of the Lysinibacillus sphaericus Tpp49Aa1 pesticidal protein elucidated from natural crystals using MHz-SFX. Proceedings of the National Academy of Sciences 120 (49) e2203241120. (10.1073/pnas.2203241120)
- Best, H. L. et al. 2023. The role of glycoconjugates as receptors for insecticidal proteins. FEMS Microbiology Reviews 47 (4)(10.1093/femsre/fuad026)
- Somogyi, A. et al., 2023. The synthetic TRPML1 agonist ML-SA1 rescues Alzheimer-related alterations of the endosomal-autophagic-lysosomal system. Journal of Cell Science 136 (6) jcs259875. (10.1242/jcs.259875)
- Fenn, G. D. et al. 2023. Development of a novel anthraquinone-derived fluorescent lysosomal probe. Molecular Genetics and Metabolism 138 (2) 107097. (10.1016/j.ymgme.2022.107097)
- Best, H. L. et al. 2022. The crystal structure of Bacillus thuringiensis Tpp80Aa1 and its interaction with galactose-containing glycolipids. Toxins 14 (12) 863. (10.3390/toxins14120863)
- Maguire, E. et al. 2021. PIP2 depletion and altered endocytosis caused by expression of Alzheimer's disease-protective variant PLCγ2 R522. EMBO Journal 40 (17) e105603. (10.15252/embj.2020105603)
- Cook, S. R. et al. 2020. Visualisation of cholesterol and ganglioside GM1 in zebrafish models of Niemann-Pick type C disease and Smith-Lemli-Opitz syndrome using light sheet microscopy. Histochemistry and Cell Biology 154 , pp.565-578. (10.1007/s00418-020-01925-2)
- Badell Grau, R. A. et al. 2020. Investigating the prevalence of reactive online searching in the COVID-19 pandemic. Journal of Medical Internet Research 22 (10) e19791. (10.2196/19791)
- Lloyd-Evans, E. and Waller-Evans, H. 2020. Biosynthesis and signalling functions of central and peripheral nervous system neurosteroids in health and disease. Essays in Biochemistry 64 (3), pp.591-606. (10.1042/EBC20200043)
- Cook, S. R. et al. 2020. Detrimental effect of zwitterionic buffers on lysosomal homeostasis in cell lines and iPSC-derived neurons. AMRC Open Research 2 21. (10.12688/amrcopenres.12903.1)
- Day, A. H. et al. 2020. Targeted cell imaging properties of a deep red luminescent iridium(III) complex conjugated with a c-Myc signal peptide. Chemical Science 11 (6), pp.1599-1606. (10.1039/C9SC05568A)
- Carreira, A. et al., 2019. Mammalian sphingoid bases: Biophysical, physiological and pathological properties. Progress in Lipid Research 75 100988. (10.1016/j.plipres.2019.100988)
- Balogi, Z. et al., 2019. Hsp70 interactions with membrane lipids regulate cellular functions in health and disease. Progress in Lipid Research 74 , pp.18-30. (10.1016/j.plipres.2019.01.004)
- Morimoto, M. et al., 2018. Bi-allelic CCDC47 variants cause a disorder characterized by woolly hair, liver dysfunction, dysmorphic features, and global developmental delay. American Journal of Human Genetics 103 (5), pp.794-807. (10.1016/j.ajhg.2018.09.014)
- Lange, J. et al., 2018. Compromised astrocyte function and survival negatively impact neurons in infantile neuronal ceroid lipofuscinosis. Acta Neuropathologica Communications 6 74. (10.1186/s40478-018-0575-4)
- Fineran, P. et al., 2017. Pathogenic mycobacteria achieve cellular persistence by inhibiting the Niemann-Pick Type C disease cellular pathway. Wellcome Open Research 1 18. (10.12688/wellcomeopenres.10036.2)
- Lloyd-Evans, E. and Haslett, L. 2016. The lysosomal storage disease continuum with ageing-related neurodegenerative disease. Ageing Research Reviews 32 , pp.104-121. (10.1016/j.arr.2016.07.005)
- Lloyd-Evans, E. 2016. On the move, lysosomal CAX drives Ca2+ transport and motility. Journal of cell biology 212 (7), pp.755-757. (10.1083/jcb.201603037)
- Oswald, M. C. W. et al., 2015. Identification of dietary alanine toxicity and trafficking dysfunction in a Drosophila model of hereditary sensory and autonomic neuropathy type 1. Human Molecular Genetics 24 (24), pp.6899-6909. (10.1093/hmg/ddv390)
- Lee, J. et al., 2015. Presenilin 1 maintains Lysosomal Ca2+ homeostasis via TRPML1 by regulating vATPase-mediated lysosome acidification. Cell Reports 12 (9), pp.1430-1444. (10.1016/j.celrep.2015.07.050)
- Chandrachud, U. et al., 2015. Unbiased cell-based screening in a neuronal cell model of Batten Disease highlights an interaction between Ca2+Homeostasis, autophagy, and CLN3 protein function. Journal of Biological Chemistry 290 (23), pp.14361-14380.. (10.1074/jbc.M114.621706)
- Waller-Evans, H. and Lloyd-Evans, E. 2015. Regulation of TRPML1 function. Biochemical Society Transactions 43 (3), pp.442-446. (10.1042/BST20140311)
- Walker, M. W. and Lloyd-Evans, E. 2015. A rapid method for the preparation of ultrapure, functional lysosomes using functionalized superparamagnetic iron oxide nanoparticles. Methods in Cell Biology 126 , pp.21-43. (10.1016/bs.mcb.2014.10.019)
- Platt, F. M. et al., 2014. Disorders of cholesterol metabolism and their unanticipated convergent mechanisms of disease. Annual Review of Genomics and Human Genetics 15 (1), pp.173-194. (10.1146/annurev-genom-091212-153412)
- Morgan, A. J. et al. 2011. Molecular mechanisms of endolysosomal Ca2+ signalling in health and disease. Biochemical Journal 439 (3), pp.349-374. (10.1042/BJ20110949)
- Lloyd-Evans, E. and Platt, F. M. 2011. Lysosomal Ca2+ homeostasis: Role in pathogenesis of lysosomal storage diseases. Cell Calcium 50 (2), pp.200-205. (10.1016/j.ceca.2011.03.010)
- Lloyd-Evans, E. and Platt, F. M. 2010. Lipids on trial: The search for the offending metabolite in Niemann-Pick type C disease. Traffic 11 (4), pp.419-428. (10.1111/j.1600-0854.2010.01032.x)
- Lloyd-Evans, E. et al. 2010. Endolysosomal calcium regulation and disease.. Biochemical Society Transactions 38 (6), pp.1458-1464. (10.1042/BST0381458)
- Ruas, M. et al., 2010. Purified TPC isoforms form NAADP receptors with distinct roles for Ca2+ signaling and endolysosomal trafficking. Current Biology 20 (8), pp.703-709. (10.1016/j.cub.2010.02.049)
- Elliot-Smith, E. et al., 2008. Beneficial effects of substrate reduction therapy in a mouse model of GM1 gangliosidosis. Molecular Genetics and Metabolism 94 (2), pp.204-211. (10.1016/j.ymgme.2008.02.005)
- Lloyd-Evans, E. et al. 2008. Niemann-Pick disease type C1 is a sphingosine storage disease that causes deregulation of lysosomal calcium. Nature Medicine 14 (11), pp.1247-1255. (10.1038/nm.1876)
- Pelled, D. et al., 2005. Enhanced calcium release in the acute neuronopathic form of Gaucher disease. Neurobiology of Disease 18 (1), pp.83-88. (10.1016/j.nbd.2004.09.004)
- Lachmann, R. H. et al., 2004. Treatment with miglustat reverses the lipid-trafficking defect in Niemann–Pick disease type C. Neurobiology of Disease 16 (3), pp.654-658. (10.1016/j.nbd.2004.05.002)
- Vruchte, D. T. et al., 2004. Accumulation of glycosphingolipids in Niemann-Pick C disease disrupts endosomal transport. Journal of Biological Chemistry 279 (25), pp.26167-26175. (10.1074/jbc.M311591200)
- Lloyd-Evans, E. et al. 2003. Glucosylceramide and glucosylsphingosine modulate calcium mobilization from brain microsomes via different mechanisms. Journal of Biological Chemistry 278 (26), pp.23594-23599. (10.1074/jbc.M300212200)
- Lloyd-Evans, E. et al. 2003. Lyso-glycosphingolipids mobilize calcium from brain microsomes via multiple mechanisms. Biochemical Journal 375 (3), pp.561-565. (10.1042/BJ20030613)
- Pelled, D. et al., 2003. Inhibition of calcium uptake via the sarco/endoplasmic reticulum Ca2+-ATPase in a mouse model of Sandhoff disease and prevention by treatment with N-Butyldeoxynojirimycin. Journal of Biological Chemistry 278 (32), pp.29496-29501. (10.1074/jbc.M302964200)
Llyfrau
- Harwood, J. L. and Lloyd-Evans, E. eds. 2020. Essays in biochemistry volume 64, issue 3: lipid mediators. Portland Press.
Ymchwil
Ein diddordebau presennol
- Ar hyn o bryd rydym yn holi nifer o dargedau lysosomaidd o fewn y grŵp ac mewn cydweithrediad ag eraill yn y MDI (Dr. Helen Waller-Evans a Dr. D. Heulyn Jones), mae hyn yn cynnwys defnyddio profion biocemegol trwybwn uchel, cemeg feddyginiaethol, bioleg strwythurol, metaboledd cyffuriau a ffarmacocineteg hyd at fodelau clefyd niwronol a microglial sy'n deillio o iPS.
- Mae prosiectau ymchwil sylfaenol yn cynnwys:
- Nodweddu sianeli ïon lysosomal newydd a nodi cyfansoddion offer newydd i fodiwleiddio eu swyddogaeth.
- Datblygu profion ffenoteipig ar gyfer galluogi sgrinio cyfansawdd trwybwn uchel yn fewnol ac yn allanol.
- Nodweddu storio lipidau a chamweithrediad lysosomal mewn DHDDS (a ariennir gan CureDHDDS).
- Datblygu dulliau AI sy'n seiliedig ar AI i alluogi darganfod cyffuriau sianel ïonau lysosomal.
- Mae'r Athro Lloyd-Evans yn gyd-fyfyriwr ar brosiect cydweithredol MDI-Asex.
Am ragor o wybodaeth fanwl am brosiectau ewch i'n gwefannau grwpiau allanol:
Sefydliad darganfod meddyginiaethauAelodau Grŵp labordy Lloyd-Evans:
- Dr Sophie Cook (Cymrawd Ymchwil)
- Dr. Hannah Best (Post-doc)
- Dr. Gaia Pasqualetto (Cynorthwy-ydd Ymchwil)
- Ms Llinos Honeybun (myfyriwr PhD)
- Mr Iwan Williams (myfyriwr PhD)
- Mr Tom Duffy (myfyriwr PhD)
Adnoddau labordy Lloyd-Evans:
Yn ogystal â'r cyfleusterau o'r radd flaenaf a natur wirioneddol amlddisgyblaethol y MDI, mae gan labordy ELE hefyd nifer o ficrosgopau unigryw a blaengar ar gyfer trwybwn uchel a delweddu confocal cyflymder uchel ochr yn ochr â Ca2+ amser real LED a systemau delweddu ïonau eraill. Rydym hefyd wedi sefydlu canolfan ragoriaeth mewn electroffisioleg awtomataidd a chlampio clytiau (gan gynnwys y Nanion Patchliner, porthladd-a-patch, Orbit a systemau SURFE2R) a dyma'r unig ganolfan sydd â'r capasiti ephys awtomataidd hwn ar draws consortiwm GW4.
Cyllid labordy Lloyd-Evans:
Rydym wedi cael ein hariannu gan MRC, BBSRC ac ERC yn ogystal â nifer o elusennau a chwmnïau. Mae'r cyllid presennol gan gwmnïau, MRC IAA, CureDHDDS, Action Medical Research, BBSRC SWBio DTP a'r Coleg Cymraeg Cenedlaethol.
Ymunwch â ni:
Mae gennym ddiddordeb bob amser mewn cefnogi ceisiadau allanol ar gyfer cymrodoriaethau neu PhD, cysylltwch â'r Athro Lloyd-Evans i drafod.
Bywgraffiad
Fy ngradd gyntaf oedd gradd israddedig mewn Biocemeg (M.Biochem) ym Mhrifysgol Caerfaddon. Yn ystod y radd hon, treuliais 11 mis (2 leoliad yn olynol) yn labordy yr Athro Tony Futerman yn Sefydliad Gwyddoniaeth Weizmann, Rehovot, Israel. Yma y cefais ddiddordeb mewn clefydau storio lysosomal, gan ymchwilio i rôl homeostasis endoplasmig (homeostasis) Ca2+ endoplasmig wedi'i newid mewn clefyd Gaucher. Yn dilyn fy ngradd yn 2002 symudais i Rydychen i wneud fy DPhil gyda'r Athro Fran Platt yn y Sefydliad Glycobiology. Yma, ymchwiliais i rôl y sffingosîn sffingolipid syml ym bathogenesis Niemann-Pick math C1. Ar ôl cwblhau fy DPhil Yn 2005, symudais gyda Fran i'r Adran Ffarmacoleg (Rhydychen) lle, mewn cydweithrediad â'r Athro Antony Galione, datblygom dechnegau i astudio homeostasis lysosomal Ca2+ yn y clefydau lysosomal. Yn 2010 cefais fy mhenodi'n Gymrawd RCUK yn Ysgol y Biowyddorau, Prifysgol Caerdydd, i barhau â'm hymchwil i swyddogaeth lysosomaidd.
