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Yr Athro Eshwar Mahenthiralingam
(e/fe)
BSc PhD FHEA
- Ar gael fel goruchwyliwr ôl-raddedig
Timau a rolau for Eshwar Mahenthiralingam
Pennaeth yr Ysgol, Ysgol y Biowyddorau
Ysgol y Biowyddorau
Trosolwyg
Trosolwg o'r ymchwil
Mae fy ngrŵp wedi astudio pathogenesis, biotechnoleg a rhyngweithiadau ecolegol pathogenau oportunistaidd bacteriol, ac ar hyn o bryd mae'n cwmpasu tair thema ryngddisgyblaethol:
- Dadansoddiadau microbiota ffibrosis systig a microbiota heintiau yr ysgyfaint
- Genomeg Burkholderia a chynhyrchu metabolit arbenigol
- Microbioleg ddiwydiannol ac ymwrthedd gwrthficrobaidd
Microbiomau, Microbau a Gwybodeg
Mae'r labordy Mahenthiralingam yn rhan o'r grŵp Microbiomau, Microbau a Gwybodeg (MMI) sydd ar hyn o bryd yn cynnwys timau ymchwil Helen Brown, Cedric Berger, Thomas Connor, Arthur Morris, Katherine Smith, Andrew Weightman a Catrin Wlliams. Rydym hefyd yn gweithio o fewn yr Is-adran Organebau a'r Amgylchedd.
Cyhoeddiad
2026
- Alswat, A. et al. 2026. Genomic diversity of culturable Paraburkholderia and Burkholderia species isolated from Bornean rainforest rhizosphere. Microbial Genomics 12 (5)(10.1099/mgen.0.001720)
- Dashti, Y. et al., 2026. Discovery and metabolic origin of 4,4ʹ-dihydroxy-3,3ʹ,5,5ʹ-tetrachlorobenzophenone from a Burkholderia oklahomensis clinical isolate. Pure and Applied Chemistry (10.1515/pac-2025-0623)
2025
- Weiser, R. et al. 2025. The fungal diversity in the lungs of children with cystic fibrosis captured by sputum-induction and bronchoalveolar lavage. Journal of Cystic Fibrosis 24 (2), pp.382-389. (10.1016/j.jcf.2024.07.011)
- Webster, G. , Mullins, A. J. and Mahenthiralingam, E. 2025. Complete genome sequence of the biopesticidal burkholderia ambifaria strain BCC0191. Microbiology Resource Announcements 14 (2) e01097-24. (10.1128/mra.01097-24)
2023
- Webster, G. et al. 2023. Polyyne-producing Burkholderia suppress Globisporangium ultimum damping-off disease of Pisum sativum (pea). Frontiers in Microbiology 14 1240206. (10.3389/fmicb.2023.1240206)
- Parfitt, K. M. et al. 2023. Identification of two distinct phylogenomic lineages and model strains for the understudied cystic fibrosis lung pathogen Burkholderia multivorans.. Microbiology 169 (8) 001366. (10.1099/mic.0.001366)
- Morgan, H. et al. 2023. A 2.8 Å structure of zoliflodacin in a DNA cleavage complex with staphylococcus aureus DNA gyrase. International Journal of Molecular Sciences 24 (2) 1634. (10.3390/ijms24021634)
2022
- Mahenthiralingam, E. et al. 2022. Selection of relevant bacterial strains for novel therapeutic testing. Current Clinical Microbiology Reports 9 , pp.33-45. (10.1007/s40588-022-00182-2)
- Petrova, Y. D. and Mahenthiralingam, E. 2022. Discovery, mode of action and secretion of Burkholderia sensu lato key antimicrobial specialised metabolites. The Cell Surface 8 100081. (10.1016/.tcsw.2022.10081)
- Cunningham-Oakes, E. et al. 2022. Novel application of metagenomics for the strain-level detection of bacterial contaminants within non-sterile industrial products – a 2 retrospective, real-time analysis. Microbial Genomics 8 (11) 000884. (10.1099/mgen.0.000884)
- Weiser, R. et al. 2022. The lung microbiota in children with cystic fibrosis captured by induced sputum sampling. Journal of Cystic Fibrosis 21 (6), pp.1006-1012. (10.1016/j.jcf.2022.01.006)
- Petrova, Y. D. et al. 2022. Cloning and expression of Burkholderia polyyne biosynthetic gene clusters in 2 Paraburkholderia hosts provides a strategy for biopesticide development. Microbial Biotechnology 15 (10), pp.2547-2561. (10.1111/1751-7915.14106)
- Roberts, A. E. L. et al., 2022. A pilot study investigating the effects of a manuka honey sinus rinse compared to a standard sinus rinse on sino-nasal outcome test scores in cystic fibrosis patients. Pilot and Feasibility Studies 8 216. (10.1186/s40814-022-01175-0)
- Fischer, R. et al., 2022. Evaluating the alginate oligosaccharide (OligoG) as a therapy for Burkholderia cepacia complex cystic fibrosis lung infection. Journal of Cystic Fibrosis 21 (5), pp.821-829. (10.1016/j.jcf.2022.01.003)
2021
- Mullins, A. J. and Mahenthiralingam, E. 2021. The hidden genomic diversity, specialised metabolite capacity, and revised taxonomy of Burkholderia sensu lato. Frontiers in Microbiology 12 726847. (10.3389/fmicb.2021.726847)
- Rushton, L. et al. 2021. Construction and evaluation of a bioluminescent Pseudomonas aeruginosa reporter for use in preservative efficacy testing. Microbiology 167 (8) 001072. (10.1099/mic.0.001072)
- Mullins, A. J. et al. 2021. Discovery of the pseudomonas polyyne protegencin by a phylogeny-guided study of polyyne biosynthetic gene cluster diversity. mBio 12 (4), pp.e00715-21. (10.1128/mBio.00715-21)
- Cunningham-Oakes, E. et al. 2021. Genomics reveals the novel species placement of industrial contaminant isolates incorrectly identified as Burkholderia lata. Microbial Genomics 7 (4) 000564. (10.1099/mgen.0.000564)
- Heath, N. L. et al., 2021. Antimicrobial activity of enacyloxin IIa and gladiolin against the urogenital pathogens 'Neisseria gonorrhoeae' and 'Ureaplasma' spp. Journal of Applied Microbiology 130 (5), pp.1546-1551. (10.1111/jam.14858)
- Cauduro, G. P. et al., 2021. New benzo(a)pyrene-degrading strains of the Burkholderia cepacia complex prospected from activated sludge in a petrochemical wastewater treatment plant. Environmental Monitoring and Assessment 193 (4) 163. (10.1007/s10661-021-08952-z)
- Oakley, J. L. et al. 2021. Phenotypic and genotypic adaptations in Pseudomonas aeruginosa biofilms following long-term exposure to an alginate oligomer therapy. mSphere 6 (1), pp.e01216-20. (10.1128/mSphere.01216-20)
- Weiser, R. , Rye, P. D. and Mahenthiralingam, E. 2021. Implementation of microbiota analysis in clinical trials for cystic fibrosis lung infection: experience from the OligoG phase 2b clinical trials. Journal of Microbiological Methods 181 106133. (10.1016/j.mimet.2021.106133)
- Jones, C. et al. 2021. Kill and cure: genomic phylogeny and bioactivity of Burkholderia gladioli bacteria capable of pathogenic and beneficial lifestyles. Microbial Genomics 17 (1) 000515. (10.1099/mgen.0.000515)
2020
- Mullins, A. J. et al. 2020. Reclassification of the biocontrol agents Bacillus subtilis BY-2 and Tu-100 as Bacillus velezensis and insights into the genomic and specialised metabolite diversity of the species. Microbiology 166 (12), pp.1121-1128. (10.1099/mic.0.000986)
- Webster, G. et al. 2020. A rapid screening method for the detection of specialised metabolites from bacteria: induction and suppression of metabolites from Burkholderia species. Journal of Microbiological Methods 178 106057. (10.1016/j.mimet.2020.106057)
- Dashti, Y. et al., 2020. Discovery and biosynthesis of bolagladins: unusual lipodepsipeptides from Burkholderia gladioli clinical isolates. Angewandte Chemie International Edition 59 (48), pp.21553-21561. (10.1002/anie.202009110)
- Rushton, L. et al. 2020. Mapping the efficacy and mode of action of ethylzingerone [4-(3-ethoxy-4-hydroxyphenyl) butan-2-one] as an active agent against Burkholderia bacteria. Applied and Environmental Microbiology 86 (19) e01808-20. (10.1128/AEM.01808-20)
- Mullins, A. J. et al. 2020. Genomic assemblies of members of Burkholderia and related genera as a resource for natural product discovery. Microbiology Resource Announcements 9 e00485-20. (10.1128/MRA.00485-20)
- Webster, G. et al. 2020. Culturable diversity of bacterial endophytes associated with medicinal plants of the Western Ghats, India. FEMS Microbiology Ecology 96 (1) fiaa147. (10.1093/femsec/fiaa147)
- Weiser, R. et al. 2020. A novel inducible prophage from Burkholderia vietnamiensis G4 is widely distributed across the species and has lytic activity against pathogenic Burkholderia. Viruses 12 (6) 601. (10.3390/v12060601)
- Cunningham-Oakes, E. et al. 2020. Genome sequence of pluralibacter gergoviae ECO77, a unique multireplicon isolate of industrial origin. Microbiology Resource Announcements 9 (9) e01561-19. (10.1128/MRA.01561-19)
2019
- Cunningham-Oakes, E. et al. 2019. Understanding the challenges of non-food industrial product contamination. FEMS Microbiology Letters 366 (23) fnaa010. (10.1093/femsle/fnaa010)
- Masschelein, J. et al., 2019. A dual transacylation mechanism for polyketide synthase chain release in enacyloxin antibiotic biosynthesis. Nature Chemistry 11 , pp.906-912. (10.1038/s41557-019-0309-7)
- Webster, G. et al. 2019. Genome sequences of three Paraburkholderia spp. strains isolated from Wood-decay fungi reveals them as novel taxa with antimicrobial biosynthetic potential. Microbiology Resource Announcements 8 (34) e00778-19. (10.1128/MRA.00778-19)
- Mullins, A. J. et al. 2019. Genome mining identifies cepacin as a plant-protective metabolite of the biopesticidal bacterium Burkholderia ambifaria. Nature Microbiology 4 , pp.996-1005. (10.1038/s41564-019-0383-z)
- Jenner, M. et al., 2019. An unusual Burkholderia gladioli double chain-initiating nonribosomal peptide synthetase assembles ‘fungal’ icosalide antibiotics. Chemical Science 10 (21), pp.5489-5494. (10.1039/C8SC04897E)
- Weiser, R. et al. 2019. Not all Pseudomonas aeruginosa are equal: strains from industrial sources possess uniquely large multireplicon genomes. Microbial Genomics 000276. (10.1099/mgen.0.000276)
- Webster, G. et al. 2019. Genome sequences of two choline-utilising methanogenic archaea, Methanococcoides spp., isolated from marine sediments. Microbiology Resource Announcements 8 (18) e00342-19. (10.1128/MRA.00342-19)
2018
- Ledwoch, K. et al. 2018. Beware Biofilm! Dry biofilms containing bacterial pathogens on multiple healthcare surfaces; a multicentre study. Journal of Hospital Infection 100 (3), pp.e47-e56. (10.1016/j.jhin.2018.06.028)
- Mitchelmore, P. J. et al., 2018. Molecular epidemiology of Pseudomonas aeruginosa in an unsegregated bronchiectasis cohort sharing hospital facilities with a cystic fibrosis cohort. Thorax 73 (7), pp.677-679. (10.1136/thoraxjnl-2016-209889)
- Ronchetti, K. et al., 2018. The CF-Sputum Induction Trial (CF-SpIT) to assess lower airway bacterial sampling in young children with cystic fibrosis: a prospective internally controlled interventional trial. Lancet Respiratory Medicine 6 (6), pp.461-471. (10.1016/s2213-2600(18)30171-1)
- Green, A. et al. 2018. The consistent differential expression of genetic pathways following exposure of an industrial Pseudomonas aeruginosa strain to preservatives and a laundry detergent formulation. FEMS Microbiology Letters 365 (9) fny062. (10.1093/femsle/fny062)
2017
- Loveridge, E. J. et al. 2017. Reclassification of the specialized metabolite producer pseudomonas mesoacidophila ATCC 31433 as a member of the burkholderia cepacia complex. Journal of Bacteriology 199 (13) e00125-17. (10.1128/JB.00125-17)
- Song, L. et al., 2017. Discovery and biosynthesis of gladiolin: a Burkholderia gladioli antibiotic with promising activity against Mycobacterium tuberculosis. Journal of the American Chemical Society 139 (23), pp.7974-7981. (10.1021/jacs.7b03382)
- Gilpin, D. F. et al., 2017. Evidence of persistence of Prevotella spp. in the cystic fibrosis lung. Journal of Medical Microbiology 66 (6), pp.825-832. (10.1099/jmm.0.000500)
2016
- Depoorter, E. et al., 2016. Burkholderia: an update on taxonomy and biotechnological potential as antibiotic producers. Applied Microbiology and Biotechnology 100 (12), pp.5215-5229. (10.1007/s00253-016-7520-x)
2015
- Cullen, L. et al., 2015. Phenotypic characterization of an international Pseudomonas aeruginosa reference panel: strains of cystic fibrosis (CF) origin show less in vivo virulence than non-CF strains. Microbiology 161 (10), pp.1961-1977. (10.1099/mic.0.000155)
- Zlosnik, J. E. A. et al., 2015. Burkholderia species infections in patients with Cystic Fibrosis in British Columbia, Canada. 30 Years' experience. Annals of the American Thoracic Society 12 (1), pp.70. (10.1513/AnnalsATS.201408-395OC)
- Flight, W. G. et al., 2015. Rapid detection of emerging pathogens and loss of microbial diversity associated with severe lung disease in cystic fibrosis. Journal of Clinical Microbiology 53 (7), pp.2022-2029. (10.1128/JCM.00432-15)
2014
- Bull, M. J. et al. 2014. The domestication of the probiotic bacterium Lactobacillus acidophilus. Scientific Reports 4 7202. (10.1038/srep07202)
- Mahenthiralingam, E. 2014. Emerging cystic fibrosis pathogens and the microbiome. Paediatric Respiratory Reviews 15 (Supp 1), pp.13-15. (10.1016/j.prrv.2014.04.006)
- Weiser, R. et al. 2014. Evaluation of five selective media for the detection of Pseudomonas aeruginosa using a strain panel from clinical, environmental and industrial sources. Journal of Microbiological Methods 99 , pp.8-14. (10.1016/j.mimet.2014.01.010)
- Vidal Quist, J. et al. 2014. Arabidopsis thaliana and Pisum sativum models demonstrate that root colonization is an intrinsic trait of Burkholderia cepacia complex bacteria. Microbiology 160 (2), pp.373-384. (10.1099/mic.0.074351-0)
2013
- Denman, C. C. et al., 2013. Growth on mannitol-rich media elicits a genome-wide transcriptional response in Burkholderia multivorans that impacts on multiple virulence traits in an exopolysaccharide-independent manner. Microbiology 160 (Pt 1), pp.187-197. (10.1099/mic.0.072975-0)
- Baxter, C. G. et al., 2013. Intravenous antibiotics reduce the presence of Aspergillus in adult cystic fibrosis sputum. Thorax 68 (7), pp.652-657. (10.1136/thoraxjnl-2012-202412)
- Bull, M. J. et al. 2013. The life history of 'Lactobacillus acidophilus' as a probiotic: a tale of revisionary taxonomy, misidentification and commercial success. FEMS Microbiology Letters 349 (2), pp.77-87. (10.1111/1574-6968.12293)
- De Soyza, A. et al., 2013. Developing an international 'Pseudomonas aeruginosa' reference panel. MicrobiologyOpen 2 (6), pp.1010-1023. (10.1002/mbo3.141)
- Knapp, L. et al. 2013. The effect of cationic microbicide exposure against Burkholderia cepacia complex (Bcc); the use of Burkholderia lata strain 383 as a model bacterium. Journal of Applied Microbiology 115 (5), pp.1117-1126. (10.1111/jam.12320)
- Rushton, L. et al. 2013. Key role for efflux in the preservative susceptibility and adaptive resistance of Burkholderia cepacia complex bacteria. Antimicrobial Agents and Chemotherapy 57 (7), pp.2972-2980. (10.1128/AAC.00140-13)
- Sass, A. et al. 2013. The unexpected discovery of a novel low-oxygen-activated locus for the anoxic persistence of Burkholderia cenocepacia. ISME Journal 7 (8), pp.1568-1581. (10.1038/ismej.2013.36)
- Van Acker, H. et al., 2013. Biofilm-grown Burkholderia cepacia complex cells survive antibiotic treatment by avoiding production of reactive oxygen species. PLoS ONE 8 (3) e58943. (10.1371/journal.pone.0058943)
- Vidal Quist, J. et al. 2013. 'Bacillus thuringiensis' colonises plant roots in a phylogeny-dependent manner. FEMS Microbiology Ecology 86 (3), pp.474-489. (10.1111/1574-6941.12175)
2012
- Bull, M. J. et al. 2012. Minimum taxonomic criteria for bacterial genome sequence depositions and announcements. Journal of Microbiological Methods 89 (1), pp.18-21. (10.1016/j.mimet.2012.02.008)
2011
- Mahenthiralingam, E. et al. 2011. Enacyloxins are products of an unusual hybrid modular polyketide synthase encoded by a cryptic burkholderia ambifaria genomic island. Chemistry & Biology 18 (5), pp.665-677. (10.1016/j.chembiol.2011.01.020)
- Bazzini, S. et al., 2011. Deciphering the role of RND efflux transporters in Burkholderia cenocepacia. PLoS ONE 6 (4) e18902. (10.1371/journal.pone.0018902)
- Coenye, T. et al., 2011. Molecular mechanisms of chlorhexidine tolerance in Burkholderia cenocepacia biofilms. Antimicrobial Agents and Chemotherapy 55 (5), pp.1912-1919. (10.1128/AAC.01571-10)
- Sass, A. et al. 2011. Spontaneous and evolutionary changes in the antibiotic resistance of Burkholderia cenocepacia observed by global gene expression analysis. BMC Genomics 12 (1) 373. (10.1186/1471-2164-12-373)
- White, J. et al. 2011. Culture-independent analysis of bacterial fuel contamination provides insight into the level of concordance with the standard industry practice of aerobic cultivation. Applied and Environmental Microbiology 77 (13), pp.4527-4538. (10.1128/AEM.02317-10)
2010
- Drevinek, P. et al., 2010. Oxidative stress of Burkholderia cenocepacia induces insertion sequence-mediated genomic rearrangements that interfere with macrorestriction-based genotyping. Journal of Clinical Microbiology 48 (1), pp.34-40. (10.1128/JCM.01433-09)
- Drevinek, P. and Mahenthiralingam, E. 2010. Burkholderia cenocepacia in cystic fibrosis: epidemiology and molecular mechanisms of virulence. Clinical Microbiology and Infection 16 (7), pp.821-830. (10.1111/j.1469-0691.2010.03237.x)
- Drevinek, P. et al., 2010. Direct Culture-Independent Strain Typing of Burkholderia cepacia Complex in Sputum Samples from Patients with Cystic Fibrosis. Journal of Clinical Microbiology 48 (5), pp.1888-1891. (10.1128/JCM.02359-09)
- Fothergill, J. L. et al., 2010. Impact of Pseudomonas aeruginosa Genomic Instability on the Application of Typing Methods for Chronic Cystic Fibrosis Infections. Journal of Clinical Microbiology 48 (6), pp.2053-2059. (10.1128/JCM.00019-10)
- McCarthy, Y. et al., 2010. A sensor kinase recognizing the cell-cell signal BDSF (cis-2-dodecenoic acid) regulates virulence in Burkholderia cenocepacia. Molecular Microbiology 77 (5), pp.1220-1236. (10.1111/j.1365-2958.2010.07285.x)
- Peeters, E. et al., 2010. Transcriptional response of Burkholderia cenocepacia J2315 sessile cells to treatments with high doses of hydrogen peroxide and sodium hypochlorite. BMC Genomics 11 90. (10.1186/1471-2164-11-90)
2009
- Holden, M. T. G. et al., 2009. The genome of Burkholderia cenocepacia J2315, an epidemic of pathogen of cystic fibrosis patients. Journal of Bacteriology 191 (1), pp.261-277. (10.1128/JB.01230-08)
- Mahenthiralingam, E. et al. 2009. Use of colony-based bacterial strain typing for tracking the fate of Lactobacillus strains during human consumption. BMC Microbiology 9 :251 (10.1186/1471-2180-9-251)
- Rose, H. et al. 2009. Biocide susceptibility of the Burkholderia cepacia complex. Journal of Antimicrobial Chemotherapy 63 (3), pp.502-510. (10.1093/jac/dkn540)
- Sass, A. , Marchbank, A. M. and Mahenthiralingam, E. 2009. Gene expression in Burkholderia Cenocepcia: The global transcriptomic response to different growth conditions encountered in the CF lung [Poster Session Abstract]. Pediatric Pulmonology 44 (S32), pp.311. (10.1002/ppul.21133)
- Schmidt, S. et al., 2009. Production of the antifungal compound pyrrolnitrin is quorum sensing-regulated in members of the Burkholderia cepacia complex. Environmental Microbiology 11 (6), pp.1422-1437. (10.1111/j.1462-2920.2009.01870.x)
- Spilker, T. et al., 2009. Expanded Multilocus Sequence Typing for Burkholderia Species. Journal of Clinical Microbiology 47 (8), pp.2607-2610. (10.1128/JCM.00770-09)
- Vanlaere, E. et al., 2009. Taxon K, a complex within the Burkholderia cepacia complex, comprises at least two novel species, Burkholderia contaminans sp. nov. and Burkholderia lata sp. nov. International Journal of Systematic and Evolutionary Microbiology 59 (1), pp.102-111. (10.1099/ijs.0.001123-0)
2008
- Drevinek, P. et al., 2008. Diversity of the parB and repA genes of the Burkholderia cepacia complex and their utility for rapid identification of Burkholderia cenocepacia. BMC Microbiology 8 44. (10.1186/1471-2180-8-44)
- Baldwin, A. et al., 2008. Elucidating global epidemiology of Burkholderia multivorans in cases of cystic fibrosis by multilocus sequence typing. Journal of Clinical Microbiology 46 (1), pp.290-295. (10.1128/JCM.01818-07)
- Cooper, I. R. et al., 2008. Long-term persistence of a single Legionella pneumophila strain possessing the mip gene in a municipal shower despite repeated cycles of chlorination. Journal of Hospital Infection 70 (2), pp.154-159. (10.1016/j.jhin.2008.06.015)
- Drevinek, P. et al., 2008. Gene expression changes linked to antimicrobial resistance, oxidative stress, iron depletion and retained motility are observed when Burkholderia cenocepacia grows in cystic fibrosis sputum. BMC Infectious Diseases 8 :121 (10.1186/1471-2334-8-121)
- Mahenthiralingam, E. , Baldwin, A. and Dowson, C. G. 2008. Burkholderia cepacia complex bacteria: opportunistic pathogens with important natural biology. Journal of Applied Microbiology 104 (6), pp.1539-1551. (10.1111/j.1365-2672.2007.03706.x)
- Marttinen, P. et al., 2008. Bayesian modeling of recombination events in bacterial populations. BMC bioinformatics 9 421. (10.1186/1471-2105-9-421)
- Sass, A. , Drevinek, P. and Mahenthiralingam, E. 2008. Antibiotic resistance in Burkholderia cenocepacia: the transciptomic response to different classes of antibiotics. Pediatric Pulmonology 43 (S31)
- Vanlaere, E. et al., 2008. Burkholderia latens sp nov., Burkholderia diffusa sp nov., Burkholderia arboris sp nov., Burkholderia seminalis sp nov and Burkholderia metallica sp nov., novel species within the Burkholderia cepacia complex. International Journal of Systematic and Evolutionary Microbiology 58 (7), pp.1580-1590. (10.1099/ijs.0.65634-0)
2007
- Coenye, T. et al., 2007. Identification of putative noncoding RNA genes in the Burkholderia cenocepacia J2315 genome. FEMS Microbiology Letters 276 (1), pp.83-92. (10.1111/j.1574-6968.2007.00916.x)
- Baldwin, A. et al., 2007. Environmental Burkholderia cepacia complex isolates in human infections. Emerging Infectious Diseases 13 (3), pp.458-461. (10.3201/eid1303.060403)
- Dalmastri, C. et al., 2007. Investigating Burkholderia cepacia complex populations recovered from Italian maize rhizosphere by multilocus sequence typing. Environmental Microbiology 9 (7), pp.1632-1639. (10.1111/j.1462-2920.2007.01273.x)
- O'Sullivan, L. A. et al. 2007. Identifying the genetic basis of ecologically and biotechnologically useful functions of the bacterium Burkholderia vietnamiensis. Environmental Microbiology 9 (4), pp.1017-1034. (10.1111/j.1462-2920.2006.01228.x)
- Waine, D. J. et al., 2007. Reliability of multilocus sequence typing of the Burkholderia cepacia complex in cystic fibrosis. Journal of Cystic Fibrosis 6 (3), pp.215-219. (10.1016/j.jcf.2006.10.003)
2006
- Payne, G. W. et al. 2006. Application of a recA gene-based identification approach to the maize rhizosphere reveals novel diversity in Burkholderia species. FEMS Microbiology Letters 259 (1), pp.126-132. (10.1111/j.1574-6968.2006.00257.x)
- Chain, P. S. G. et al., 2006. Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility. Proceedings of the National Academy of Sciences of the United States of America 103 (42), pp.15280-15287. (10.1073/pnas.0606924103)
- Mahenthiralingam, E. et al. 2006. Multilocus sequence typing breathes life into a microbial metagenome. PLoS ONE 1 (1) e17. (10.1371/journal.pone.0000017)
- Taylor, C. J. and Mahenthiralingam, E. 2006. Functional foods and paediatric gastro-intestinal health and disease. Annals of Tropical Paediatrics 26 (2), pp.79-86. (10.1179/146532806X107403)
2005
- Lewis, D. A. et al., 2005. Identification of DNA markers for a transmissible pseudomonas aeruginosa cystic fibrosis strain. American Journal of Respiratory Cell and Molecular Biology Vol 33 (1), pp.56-64. (10.1165/rcmb.2004-0352OC)
- Baldwin, A. et al., 2005. Multilocus sequence typing scheme that provides both species and strain differentiation for the Burkholderia cepacia complex. Journal of Clinical Microbiology 43 (9), pp.4665-4673. (10.1128/JCM.43.9.4665-4673.2005)
- Drevinek, P. et al. 2005. Widespread clone of Burkholderia cenocepacia in cystic fibrosis patients in the Czech Republic. Journal of Medical Microbiology 54 (7), pp.655-659. (10.1099/jmm.0.46025-0)
- Jones, B. V. et al. 2005. Role of swarming in the formation of crystalline Proteus mirabilis biofilms on urinary catheters. Journal of Medical Microbiology 54 (9), pp.807-813. (10.1099/jmm.0.46123-0)
- Mahenthiralingam, E. , Urban, T. A. and Goldberg, J. B. 2005. The multifarious, multireplicon Burkholderia cepacia complex. Nature Reviews. Microbiology 3 (2), pp.144-156. (10.1038/nrmicro1085)
- Mahenthiralingam, E. and Vandamme, P. 2005. Taxonomy and pathogenesis of the Burkholderia cepacia complex. Chronic Respiratory Disease 2 (4), pp.209-217. (10.1191/1479972305cd053ra)
- Malott, R. J. et al., 2005. Characterization of the cciIR Quorum-sensing system in Burkholderia cenocepacia. Infection and Immunity 73 (8), pp.4982-4992. (10.1128/IAI.73.8.4982-4992.2005)
- O'Sullivan, L. A. and Mahenthiralingam, E. 2005. Biotechnological potential within the genus Burkholderia. Letters in Applied Microbiology 41 (1), pp.8-11. (10.1111/j.1472-765X.2005.01758.x)
- Payne, G. W. et al. 2005. Development of a recA gene-based identification approach for the entire Burkholderia genus. Applied and Environmental Microbiology 71 (7), pp.3917-3927. (10.1128/AEM.71.7.3917-3927.2005)
2004
- Codling, C. E. et al., 2004. Identification of genes involved in the susceptibility of Serratia marcescens to polyquaternium-1. Journal of Antimicrobial Chemotherapy 54 (2), pp.370-375. (10.1093/jac/dkh351)
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- Mahenthiralingam, E. , Baldwin, A. and Dowson, C. G. 2008. Burkholderia cepacia complex bacteria: opportunistic pathogens with important natural biology. Journal of Applied Microbiology 104 (6), pp.1539-1551. (10.1111/j.1365-2672.2007.03706.x)
- Marttinen, P. et al., 2008. Bayesian modeling of recombination events in bacterial populations. BMC bioinformatics 9 421. (10.1186/1471-2105-9-421)
- Sass, A. , Drevinek, P. and Mahenthiralingam, E. 2008. Antibiotic resistance in Burkholderia cenocepacia: the transciptomic response to different classes of antibiotics. Pediatric Pulmonology 43 (S31)
- Vanlaere, E. et al., 2008. Burkholderia latens sp nov., Burkholderia diffusa sp nov., Burkholderia arboris sp nov., Burkholderia seminalis sp nov and Burkholderia metallica sp nov., novel species within the Burkholderia cepacia complex. International Journal of Systematic and Evolutionary Microbiology 58 (7), pp.1580-1590. (10.1099/ijs.0.65634-0)
- Coenye, T. et al., 2007. Identification of putative noncoding RNA genes in the Burkholderia cenocepacia J2315 genome. FEMS Microbiology Letters 276 (1), pp.83-92. (10.1111/j.1574-6968.2007.00916.x)
- Baldwin, A. et al., 2007. Environmental Burkholderia cepacia complex isolates in human infections. Emerging Infectious Diseases 13 (3), pp.458-461. (10.3201/eid1303.060403)
- Dalmastri, C. et al., 2007. Investigating Burkholderia cepacia complex populations recovered from Italian maize rhizosphere by multilocus sequence typing. Environmental Microbiology 9 (7), pp.1632-1639. (10.1111/j.1462-2920.2007.01273.x)
- O'Sullivan, L. A. et al. 2007. Identifying the genetic basis of ecologically and biotechnologically useful functions of the bacterium Burkholderia vietnamiensis. Environmental Microbiology 9 (4), pp.1017-1034. (10.1111/j.1462-2920.2006.01228.x)
- Waine, D. J. et al., 2007. Reliability of multilocus sequence typing of the Burkholderia cepacia complex in cystic fibrosis. Journal of Cystic Fibrosis 6 (3), pp.215-219. (10.1016/j.jcf.2006.10.003)
- Payne, G. W. et al. 2006. Application of a recA gene-based identification approach to the maize rhizosphere reveals novel diversity in Burkholderia species. FEMS Microbiology Letters 259 (1), pp.126-132. (10.1111/j.1574-6968.2006.00257.x)
- Chain, P. S. G. et al., 2006. Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility. Proceedings of the National Academy of Sciences of the United States of America 103 (42), pp.15280-15287. (10.1073/pnas.0606924103)
- Mahenthiralingam, E. et al. 2006. Multilocus sequence typing breathes life into a microbial metagenome. PLoS ONE 1 (1) e17. (10.1371/journal.pone.0000017)
- Taylor, C. J. and Mahenthiralingam, E. 2006. Functional foods and paediatric gastro-intestinal health and disease. Annals of Tropical Paediatrics 26 (2), pp.79-86. (10.1179/146532806X107403)
- Lewis, D. A. et al., 2005. Identification of DNA markers for a transmissible pseudomonas aeruginosa cystic fibrosis strain. American Journal of Respiratory Cell and Molecular Biology Vol 33 (1), pp.56-64. (10.1165/rcmb.2004-0352OC)
- Baldwin, A. et al., 2005. Multilocus sequence typing scheme that provides both species and strain differentiation for the Burkholderia cepacia complex. Journal of Clinical Microbiology 43 (9), pp.4665-4673. (10.1128/JCM.43.9.4665-4673.2005)
- Drevinek, P. et al. 2005. Widespread clone of Burkholderia cenocepacia in cystic fibrosis patients in the Czech Republic. Journal of Medical Microbiology 54 (7), pp.655-659. (10.1099/jmm.0.46025-0)
- Jones, B. V. et al. 2005. Role of swarming in the formation of crystalline Proteus mirabilis biofilms on urinary catheters. Journal of Medical Microbiology 54 (9), pp.807-813. (10.1099/jmm.0.46123-0)
- Mahenthiralingam, E. , Urban, T. A. and Goldberg, J. B. 2005. The multifarious, multireplicon Burkholderia cepacia complex. Nature Reviews. Microbiology 3 (2), pp.144-156. (10.1038/nrmicro1085)
- Mahenthiralingam, E. and Vandamme, P. 2005. Taxonomy and pathogenesis of the Burkholderia cepacia complex. Chronic Respiratory Disease 2 (4), pp.209-217. (10.1191/1479972305cd053ra)
- Malott, R. J. et al., 2005. Characterization of the cciIR Quorum-sensing system in Burkholderia cenocepacia. Infection and Immunity 73 (8), pp.4982-4992. (10.1128/IAI.73.8.4982-4992.2005)
- O'Sullivan, L. A. and Mahenthiralingam, E. 2005. Biotechnological potential within the genus Burkholderia. Letters in Applied Microbiology 41 (1), pp.8-11. (10.1111/j.1472-765X.2005.01758.x)
- Payne, G. W. et al. 2005. Development of a recA gene-based identification approach for the entire Burkholderia genus. Applied and Environmental Microbiology 71 (7), pp.3917-3927. (10.1128/AEM.71.7.3917-3927.2005)
- Codling, C. E. et al., 2004. Identification of genes involved in the susceptibility of Serratia marcescens to polyquaternium-1. Journal of Antimicrobial Chemotherapy 54 (2), pp.370-375. (10.1093/jac/dkh351)
- Jones, B. V. et al. 2004. Ultrastructure of Proteus mirabilis swarmer cell rafts and role of swarming in catheter-associated urinary tract infection. Infection and Immunity 72 (7), pp.3941-3950. (10.1128/IAI.72.7.3941-3950.2004)
- Baldwin, A. et al. 2004. The Burkholderia cepacia epidemic strain marker is part of a novel genomic island encoding both virulence and metabolism-associated genes in Burkholderia cenocepacia. Infection and Immunity 72 (3), pp.1537-1547. (10.1128/IAI.72.3.1537-1547.2004)
- Engledow, A. S. et al., 2004. Involvement of a plasmid-encoded type IV secretion system in the plant tissue watersoaking phenotype of Burkholderia cenocepacia. Journal of Bacteriology 186 (18), pp.6015-6024. (10.1128/JB.186.18.6015-6024.2004)
- McDowell, A. et al., 2004. Epidemiology of Burkholderia cepacia complex species recovered from cystic fibrosis patients: issues related to patient segregation. Journal of Medical Microbiology 53 (7), pp.663-668. (10.1099/jmm.0.45557-0)
- Sabbuba, N. A. et al. 2004. Genotyping demonstrates that the strains of Proteus mirabilis from bladder stones and catheter encrustations of patients undergoing long-term bladder catheterization are identical. Journal of Urology 171 (5), pp.1925-1928. (10.1097/01.ju.0000123062.26461.f9)
- Storms, V. et al., 2004. Polyphasic characterisation of Burkholderia cepacia-like isolates leading to the emended description of Burkholderia pyrrocinia. Systematic and Applied Microbiology 27 (5), pp.517-526. (10.1078/0723202041748190)
- Vermis, K. et al., 2004. Proposal to accommodate Burkholderia cepacia genomovar VI as Burkholderia dolosa sp. nov.. International Journal of Systematic and Evolutionary Microbiology 54 (3), pp.689-691. (10.1099/ijs.0.02888-0)
- Dalmastri, C. et al., 2003. A rhizospheric Burkholderia cepacia complex population: genotypic and phenotypic diversity of Burkholderia cenocepacia and Burkholderia ambifaria. FEMS Microbiology Ecology 46 (2), pp.179-187. (10.1016/S0168-6496(03)00211-3)
- Fraud, S. et al., 2003. Aromatic alcohols and their effect on Gram-negative bacteria, cocci and mycobacteria. Journal of Antimicrobial Chemotherapy 51 (6), pp.1435-1436. (10.1093/jac/dkg246)
- Sabbuba, N. A. , Mahenthiralingam, E. and Stickler, D. J. 2003. Molecular epidemiology of Proteus mirabilis infections of the catheterized urinary tract. Journal of Clinical Microbiology 41 (11), pp.4961-4965. (10.1128/JCM.41.11.4961-4965.2003)
- Vandamme, P. et al., 2003. Burkholderia cenocepacia sp. nov.—a new twist to an old story. Research in Microbiology 154 (2), pp.91-96. (10.1016/S0923-2508(03)00026-3)
- Speert, D. P. et al., 2002. Epidemiology of Pseudomonas aeruginosa in Cystic Fibrosis in British Columbia, Canada. American Journal of Respiratory and Critical Care Medicine , pp.988-993. (10.1164/rccm.2203011)
- Agodi, A. et al., 2002. Burkholderia cepacia complex in cystic fibrosis and non-cystic fibrosis patients: identification of a cluster of epidemic lineages. Journal of Hospital Infection 50 (3), pp.188-195. (10.1053/jhin.2001.1160)
- Greenberg, D. et al., 2002. Emergence of penicillin-nonsusceptible Streptococcus pneumoniae invasive clones in Canada. Journal of Clinical Microbiology 40 (1), pp.68-74. (10.1128/JCM.40.1.68-74.2002)
- Mahenthiralingam, E. , Baldwin, A. and Vandamme, P. 2002. Burkholderia cepacia complex infection in patients with cystic fibrosis. Journal of Medical Microbiology 51 (7), pp.533-538.
- Soni, R. et al., 2002. Effect of Burkholderia cepacia infection in the clinical course of patients with cystic fibrosis: a pilot study in a Sydney clinic. Respirology 7 (3), pp.241-245. (10.1046/j.1440-1843.2002.00387.x)
- Speert, D. P. et al., 2002. Epidemiology of Burkholderia cepacia complex in patients with cystic fibrosis, Canada. Emerging Infectious Diseases 8 (2), pp.181-187. (10.3201/eid0802.010163)
- Speert, D. P. et al., 2002. Epidemiology of Burkholderia cepacia complex in patients with cystic fibrosis, Canada [Erratum]. Emerging infectious diseases 8 (5), pp.540. (10.3201/eid0805.C20805)
- Vandamme, P. et al., 2002. Burkholderia anthina sp. nov. and Burkholderia pyrrocinia, two additional Burkholderia cepacia complex bacteria, may confound results of new molecular diagnostic tools. FEMS Immunology & Medical Microbiology 33 (2), pp.143-149. (10.1016/S0928-8244(02)00301-2)
- Vermis, K. et al., 2002. Evaluation of species-specific recA-based PCR tests for genomovar level identification within the Burkholderia cepacia complex. Journal of Medical Microbiology 51 (11), pp.937-940.
- Mahenthiralingam, E. et al. 2001. Infection with Burkholderia cepacia complex genomovars in patients with cystic fibrosis: Virulent transmissible strains of genomovar III can replace Burkholderia multivorans. Clinical Infectious Diseases 33 (9), pp.1469-1475. (10.1086/322684)
- Agodi, A. et al., 2001. Burkholderia cepacia complex infection in Italian patients with cystic fibrosis: prevalence, epidemiology, and genomovar status. Journal of Clinical Microbiology 39 (8), pp.2891-2896. (10.1128/JCM.39.8.2891-2896.2001)
- Coenye, T. et al., 2001. Burkholderia ambifaria sp. nov., a novel member of the Burkholderia cepacia complex including biocontrol and cystic fybrosis-related isolates. International Journal of Systematic and Evolutionary Microbiology 51 (4), pp.1481-1490. (10.1099/00207713-51-4-1481)
- De Soyza, A. et al., 2001. Burkholderia cepacia complex genomovars and pulmonary transplantation outcomes in patients with cystic fibrosis. The Lancet 358 (9295), pp.1780-1781. (10.1016/S0140-6736(01)06808-8)
- Henry, D. A. et al., 2001. Phenotypic methods for determining genomovar status of the Burkholderia cepacia complex. Journal of Clinical Microbiology 39 (3), pp.1073-1078. (10.1128/JCM.39.3.1073-1078.2001)
- LiPuma, J. J. et al., 2001. Disproportionate distribution of Burkholderia cepacia complex species and transmissibility markers in cystic fibrosis. American Journal of Respiratory and Critical Care Medicine 164 (1), pp.92-96. (10.1164/ajrccm.164.1.2011153)
- McDowell, A. et al., 2001. PCR-Based detection and identification of Burkholderiacepacia complex pathogens in sputum from cystic fibrosis patients. Journal of Clinical Microbiology 39 (12), pp.4247-4255. (10.1128/JCM.39.12.4247-4255.2001)
- Siddiqui, A. H. et al., 2001. An episodic outbreak of genetically related Burkholderia cepacia among non-cystic fibrosis patients at a university hospital. Infection Control and Hospital Epidemiology 22 (7), pp.419-422. (10.1086/501927)
- Campbell, M. , Mahenthiralingam, E. and Speert, D. P. 2000. Evaluation of random amplified polymorphic DNA typing of Pseudomonas aeruginosa. Journal of Clinical Microbiology 38 (12), pp.4614-4615.
- Mahenthiralingam, E. et al. 2000. DNA-based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III. Journal of Clinical Microbiology 38 (9), pp.3165-3173.
- Vandamme, P. et al., 2000. Identification and population structure of Burholderia stabilis sp.nov. (formerly Burkholderia cepacia genomovar IV). Journal of Clinical Microbiology 38 (3), pp.1042-1047.
Ymchwil
Cystic fibrosis (CF) microbiology and lung infection microbiota analyses
We are examining the pathogenesis, antimicrobial resistance, microbiota interactions and genomics of Pseudomonas aeruginosa and Burkholderia bacteria, which cause problematic lung infections in people with cystic fibrosis. My early career expertise and studies are outlined under the bibliography section; current projects are described below
Microbiota analysis of CF lung infections
We successfully applied a simple bacterial microbiota-profiling PCR to CF sputum samples and showed it could rapidly detect emerging antibiotic resistant pathogens such as Burkholderia, Achromobacter and Stenotrophomonas (see Flight et al. 2015). In collaboration with Julian Forton, we found that the microbiota of induced sputum (a safe sampling method for children with CF who do not produce sputum) contained bacterial diversity that was representative of their lung infections and overlapped sampling by bronchoalveolar lavage (the gold standard but invasive sampling procedure) (see Ronchetti et al. 2018). We have also worked with AlgiPharma AS to track microbiota changes in the CF lung during their clinical trial of Oligo G, a novel anti-infective therapeutic (see Weiser et al. 2021).
Phylogenomics of Pseudomonas aeruginosa and Burkholderia bacteria
We have been involved in collaborative studies to define and evaluate panels of representative P. aeruginosa strains (Cullen et al. 2015) and recently mapped the phylogenomics of this species (Weiser and Green et al. 2019). Our genomic characterisation of Burkholderia has been extensive, recently releasing a dataset of 450 genomes (Mullins et al. 2020) and characterising them in a number of studies (see below).
Burkholderia genomics and specialised metabolite production
We have undertaken studies examining the pathogenomics of Burkholderia bacteria. Highlights include:
Pathogenomics of Burkholderia in CF
We published the first complete genome for the Burkholderia cenocepacia strain J2315 (Holden et al. 2009). We also undertook the first global gene expression analyses B. cenocepacia grown CF sputum (see Drevinek et al. 2008). Using a B. cenocepacia microarray, we mapped the evolution of antibiotic resistance in B. cenocepacia (Sass et al. 2011), and subsequently discovered the low oxygen regulated locus which enables persistence of this pathogen in the oxygen-deprived CF lung (Sass et al. 2013).
Genome mining Burkholderia for specialised metabolites and novel antibiotics
In 2007, we began screening our large collection of Burkholderia bacteria for the production of novel antibiotics. This led to the discovery of the polyketide antibiotic enacyloxin IIa and its unique biosynthetic pathway in Burkholderia ambifaria (Mahenthiralingam et al. 2011). Since 2014, we have led a successful BBSRC-funded interdisciplinary collaboration with Prof Greg Challis (University of Warwick), to characterise novel specialized metabolites produced by Burkholderia bacteria. After characterising enacyloxin, our interdisciplinary teams have followed up with discoveries of:
- Gladiolin, a novel macrolide capable of killing Mycobacterium tuberculosis (Song et al. 2017)
- Cepacin, a potent polyyne antibiotic and core component of the biopesticidal ability of Burkholderia to protect crops plants against pathogen attack (Mullins et al. 2019). The discovery of the biopesticidal activity of cepacin resulted from interdisciplinary collaborations with Prof. Jim Murray and his plant research group.
- Iscosalides produced by Burkholderia gladioli (Jenner et al. 2019)
- Unique enacyloxin biosynthesis mechanisms that open up possibilities for molecular engineering of this potent antibiotic (Masschelein et al. 2019)
- Novel bolagladin lipodepsipeptide metabolites produced by Burkholderia gladioli that can bind iron (Dashti et al. 2020)
- The novel Burkholderia glutarimide antibiotic, gladiostatin, with anticancer activity (Nakou et al. 2020).
- The enormous potential within a collection of 450 genomes we have released to aid specialized metabolite discovery in Burkholderia bacteria (Mullins et al. 2020)
- The diversity of potentially beneficial and toxic specialized metabolites encoded and expressed by the CF pathogen, Burkholderia gladioli (Jones et al. 2021)
Industrial microbiology and antimicrobial resistance
Pseudomonas and Burkholderia bacteria have high intrinsic antimicrobial resistance and can occasionally overcome preservative formulations, causing contamination in a range of non-sterile industrial products. We have worked with a number of commercial sponsors to understand industrial microbiology problems as follows.
Bacterial contamination in non-sterile industrial products
We have also been working with Unilever Research and Development (Port Sunlight) and their Safety and Environmental Assurance Centre (SEAC; Colworth) examining both Burkholderia and Pseudomonas bacteria as objectionable contaminant micro-organisms that occur in industry. We showed that multiple B. cepacia complex species can cause industrial contamination and use efflux as a key preservative resistance mechanism (see Rushton et al. 2013). We mapped the global transcriptomic responses of P. aeruginosa to preservatives (Green et al. 2018) and showed for the first time that strains from industry have the largest genomes and megaplasmids found in this antibiotic resistant species (Weiser and Green et al. 2019). Using molecular approaches we have also shown that ethylzingerone is a highly effective preservative that kills Burkholderia bacteria by novel mechanisms (Ruston et al. 2020).
Improving the reporting and identification of antimicrobial resistant industrial contaminants
Current guidelines used in the manufacture of non-sterile product do not necessarily require reporting or complete identification of bacteria which overcome preservation and cause contamination. We have shown that nearly 50% industrial product recall reports within Europe do not identify the causative organism, yet when contaminants are identified they constitute antibiotic resistant bacteria such as Pseudomonas, Burkholderia and Enterobacteriaceae (Cunningham-Oakes et al. 2019). We are now using genomic taxonomy methods to accurately identify industrial contaminants (Cunningham-Oakes et al. 2020).
Current Collaborators
International
International Burkholderia cepacia Working Group (IBCWG)
Prof. Peter Vandamme and Prof. Tom Coenye, University of Gent, Gent, Belgium
Prof. John LiPuma, University of Michigan, Ann Arbor, Michigan USA
National
Andrew Weightman, Tom Connor, Cedric Berger, Julian Marchesi (now at Imperial) and Jim Murray, Cardiff School of Biosciences, and Julian Forton, Cardiff School of Medicine, Cardiff University
Gregory Challis, Jinlian Zhao and Matthew Jenner, Department of Chemistry, University of Warwick, Coventry
Julian Parkhill, The Wellcome Trust Sanger Institute, Hinxton, Cambridge
Andy Bailey, University of Bristol
Unilever Research and Development (Port Sunlight) and Unilever Safety and Environmental Assurance Centre (SEAC; Colworth)
Grants
Biology and Biotechnology Research Council
Unilever Research and Development, UK
The US Cystic Fibrosis Foundation
The Sêr Cymru II Welsh Government Fellowship Scheme
Current group members
Dr. Rebecca Weiser ([email protected])
Dr. Gordon Webster ([email protected])
Dr. Alex Mullins ([email protected])
Dr. Laura Rushton ([email protected])
Mr. Abdullah Aseeri ([email protected])
Ms. Kasia Parfitt ([email protected])
Ms. Yoana Petrova ([email protected])
Past postgraduate trainees
PhD. Dr. Edward Cunningham-Oakes, Dr. Amal Alswat, Dr. Angharad Green, Dr. Matthew Bull, Dr. Othman Boaisha, Dr. Judith White, Dr. Helen Rose, Dr. George Payne, Dr. Saber Yezli, and Dr. Brian Jones.
Masters. Ms. Laura Evans, Ms. Fiona Lugg, Mr. Christopher Paisey, Ms. Alice Collins and Mr. Nico Bruyniks.
Past group members
Dr. Andrea Sass, Dr. Pavel Drevinek, Dr. Louise O'Sullivan, Dr. Adam Baldwin, Dr. Deborah Lewis, and Ms. Angela Marchbank
Addysgu
Rwy'n cynnal ystod o addysgu o Flwyddyn 1 (lefel 4) i Flwyddyn Olaf (lefel 6) israddedig, gan gynnwys goruchwylio myfyrwyr prosiect israddedig blwyddyn olaf a phrosiect Meistr Integredig (lefel 7).
Mae'r addysgu presennol yn cynnwys cyfraniadau at:
BI1003 Organebau a'r Amgylchedd - Cyflwyniad i facteria sy'n berthnasol yn feddygol
BI2332 Cysyniadau Clefyd - Darlith ac ymarferion ar achosion o haint bacteriol.
BI2132 Geneteg a'i Gymwysiadau - Cysyniadau ac enghreifftiau mewn esblygiad bacteriol a geneteg.
Prosiectau Ymchwil Blwyddyn Olaf a Myfyrwyr Meistr Integredig - Rwy'n hapus i ddatblygu a chynnal prosiectau ymchwil ar gyfer myfyrwyr israddedig yn seiliedig ar fy meysydd ymchwil cyfredol (gweler Ymchwil) a gallaf deilwra'r rhain ar gyfer myfyrwyr biofeddygol, biolegol, biocemegol neu geneteg oherwydd natur ryngddisgyblaethol fy arbenigedd.
Bywgraffiad
Trosolwg
Ar hyn o bryd rwy'n arwain Ysgol y Biowyddorau ym Mhrifysgol Caerdydd ac yn arbenigo mewn microbioleg foleciwlaidd, gyda ffocws penodol ar pathogenau microbaidd sy'n effeithio ar bobl â ffibrosis systig (CF). Mae fy ymchwil wedi helpu i lunio dealltwriaeth fyd-eang o Pseudomonas aeruginosa a'r cymhleth Burkholderia cepacia, gan ddatgelu sut mae'r bacteria hyn yn addasu, lledaenu ac esblygu mewn lleoliadau clinigol ac amgylcheddol.
Taith Academaidd
Dechreuais fy nhaith academaidd yng Nghaerdydd, gan ennill gradd Anrhydedd Dosbarth Cyntaf mewn Bioleg Gymhwysol, ac yna PhD mewn Geneteg Mycobacteriol yn y Sefydliad Cenedlaethol ar gyfer Ymchwil Feddygol yn Llundain. Dros y blynyddoedd, rwyf wedi dal sawl rôl arweinyddiaeth, gan gynnwys Cyd-Gyfarwyddwr Ymchwil ar gyfer yr Ysgol Biowyddoniaeth, ac REF2021 Arweinydd Uned ar gyfer y Gwyddorau Biolegol ym Mhrifysgol Caerdydd. Yn allanol, rwyf wedi gweithio gyda'r Cyngor Ymchwil Biotechnoleg a Gwyddorau Biolegol (BBSRC) fel adolygydd panel grant ac yna'n Ddirprwy Gadeirydd Panel B (Planhigion, microbau, bwyd a chynaliadwyedd) tan fis Ebrill 2025.
Ymchwil
Mae fy ymchwil wedi cyfrannu at ddatblygu diagnosteg DNA cyflym, olrhain epidemiolegol byd-eang, ac offer teipio moleciwlaidd ar gyfer pathogenau CF bacteriol. Mae'r datblygiadau hyn wedi cefnogi dosbarthu rhywogaethau bacteriol newydd ac adnabod ynysoedd pathogenedd newydd. Dros y blynyddoedd mae hyn hefyd wedi ehangu i ymchwil biotechnolegol i ddarganfod gwrthfiotigau newydd, datblygu dulliau ar gyfer bioreoli pathogenau cnydau, ac atal halogiad microbaidd mewn diwydiant.
Mentora a Hyfforddiant
O fewn y byd academaidd, rwyf wedi hyfforddi nifer o fyfyrwyr PhD ac wedi archwilio traethodau ôl-raddedig yn genedlaethol ac yn rhyngwladol. Rwyf wedi gwasanaethu fel arholwr allanol ar gyfer rhaglenni MSc ac yn parhau i arwain a mentora gwyddonwyr ar ddechrau gyrfa. Hyrwyddo microbioleg a genomeg, a datrys problemau sy'n gysylltiedig â microbau yw'r hyn sy'n fy nghyffroi, ac mae'n wych cefnogi'r genhedlaeth nesaf o ymchwilwyr sy'n hyfforddi yn y meysydd hyn.
Anrhydeddau a dyfarniadau
- Cymrodoriaeth yr Academi Addysg Uwch (FHEA; Mai 2023)
- Diploma Lefel 5 mewn Rheolaeth Brifysgol Ymarferol (Mehefin 2015)
- Cadair mewn Microbioleg Moleciwlaidd (Prifysgol Caerdydd, Awst 2011)
- Gwobr y Llywyddion, Gwobr teithio ymchwil y Gymdeithas Microbioleg Gymhwysol (Awst 2008)
- Gwobr deithio ymchwilydd cyfnod cynnar Prifysgol Caerdydd (Awst 2006; ymchwilydd gwadd ym Mhrifysgol Harvard a'r Broad Institute, Sefydliad Technoleg Masschusetts, Boston, UDA)
- Gwobr Datblygu Gyrfa; Cymdeithas Ysgyfaint British Columbia (2 flynedd o Hydref 1997)
- Ysgoloriaeth Ymchwil, Cyngor Ymchwil Feddygol Canada (5 mlynedd, o 1999 ymlaen; gwrthododd ddechrau swydd barhaol ym Mhrifysgol Caerdydd)
- Cymrodoriaeth, Cymdeithas Ysgyfaint British Columbia; Sefydliad Ymchwil BC ar gyfer Iechyd Plant a Theuluoedd (1 flwyddyn o Orffennaf 1997)
- Cymrodoriaeth Ymchwil Ôl-ddoethurol; Sefydliad Ffibrosis Systig Canada (3 blynedd; o fis Ionawr 1991)
Aelodaethau proffesiynol
- European Cystic Fibrosis Society (2009 onwards). Member of Annual Conference Microbiology Assembly Planning Group (leader for Microbiology 2013 to 2015) and invited serve on the annual conference steering committee from 2016 to 2019.
- Microbiology Society (1999 onwards)
- Society for Applied Microbiology (1999 onwards)
- American Society for Microbiology (1990 onwards)
Safleoedd academaidd blaenorol
- Reader (2007- August 2011), Cardiff School of Biosciences, Cardiff University
- Senior Lecturer (2003-2007; address as above)
- Lecturer Grade (1999-2003; address as above)
- Assistant Professor (1997-1999), Department of Paediatrics, Faculty of Medicine, University of British Columbia, Vancouver, Canada
- Research Associate (1995-1997; address as above)
- Canadian CF Foundation Postdoctoral Research Fellow (1991-1995; address as above)
Pwyllgorau ac adolygu
- 2000-2008 Journal of Clinical Microbiology, tymor 8 mlynedd fel aelod llawn o'r bwrdd golygyddol
- Panel B BBSRC Planhigion, microbau, bwyd a chynaliadwyedd: aelod o'r gronfa (Ionawr 2017 i Hydref 2018); aelod craidd (Tachwedd 2018 i Ionawr 2022), a Dirprwy Gadeirydd (Chwefror 2022 i Ebrill 2025)
- Adolygydd gwadd: Sefydliad Ffibrosis Systig yr Unol Daleithiau, Pwyllgor Ymchwil a Hyfforddiant Mawrth 2009 ymlaen; Ffibrosis Systig Canada, aelod o'r Panel Adolygu Ymchwil, 2005 ymlaen; Cyngor Ymchwil Hong Kong, panel adolygu grantiau allanol o 2012 ymlaen. Adolygiadau gwahoddedig ar gyfer: Ymddiriedolaeth Ffibrosis Systig, Sefydliadau Ymchwil Iechyd Canada, Ymddiriedolaeth Wellcome, MRC, a NERC
Meysydd goruchwyliaeth
Mae gen i ddiddordeb mewn goruchwylio myfyrwyr ôl-raddedig sydd â diddordeb yn y meysydd prosiect canlynol:
- Microbioleg Moleciwlaidd
- Genomeg, pathogenesis ac ecoleg clefydau heintus bacteriol
- Micro-organebau sy'n cynhyrchu gwrthfiotigau a darganfod metabolit arbenigol
- Deall a brwydro yn erbyn ymwrthedd gwrthficrobaidd mewn bacteria
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- Dadansoddiad cymunedol microbaidd mewn clefydau heintus a'r amgylchedd naturiol
Goruchwyliaeth gyfredol
Brooklyn Rowlands
Ymgysylltu
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Contact Details
[email protected]
+44 29208 75875
Adeilad Syr Martin Evans, Llawr Trydydd, Ystafell Dwyrain 3.32, Rhodfa'r Amgueddfa, Caerdydd, CF10 3AX
+44 29208 75875
Adeilad Syr Martin Evans, Llawr Trydydd, Ystafell Dwyrain 3.32, Rhodfa'r Amgueddfa, Caerdydd, CF10 3AX
Themâu ymchwil
Arbenigeddau
- Rheoli Heintiau
- ymwrthedd gwrthficrobaidd
- Biotechnoleg amaethyddol
