![David Petrik](https://profiles-cardiff.imgix.net/attachments/1168?w=200&h=200&auto=format&crop=faces&fit=crop&q=90")
Dr David Petrik
Uwch Ddarlithydd
Ysgol y Biowyddorau
- PetrikD@caerdydd.ac.uk
- +44 29208 79391
- Adeilad Syr Martin Evans, Rhodfa'r Amgueddfa, Caerdydd, CF10 3AX
- Ar gael fel goruchwyliwr ôl-raddedig
Trosolwyg
My research focuses on adult neural stem cells and adult neurogenesis. Neural stem cells in the adult mammalian brain have the capacity to generate new neurons, which are important for brain functions such as learning and memory, mood control, and body metabolism. I am interested in studying factors that regulate the stem cells and brain functions that are regulated by the stem cells and their cell progeny. I am particularly interested in determining how aberrant diet can influence the stem cells and how stem cells can regulate the food intake behaviour and development of obesity.
Interested in joining my research group?
I enjoy supervising students. Research in my group is suitable for undergraduate, Master's and PhD students. I am open to discussion about fellowship applications with postdoctoral candidates. If you are interested in joining the lab, please contact me by email.
Cyhoeddiad
2024
- Jörgensen, S. K. M. et al. 2024. An analogue of the Prolactin Releasing Peptide reduces obesity and promotes adult neurogenesis. EMBO Reports 25(1), pp. 351-377. (10.1038/s44319-023-00016-2)
2022
- Petrik, D., Jorgensen, S., Eftychidis, V. and Siebzehnrubl, F. A. 2022. Singular adult neural stem cells do not exist. Cells 11(4), article number: 722. (10.3390/cells11040722)
2021
- Gupta, B. et al. 2021. The transcription factor ZEB1 regulates stem cell self-renewal and cell fate in the adult hippocampus. Cell Reports 36(8), article number: 109588. (10.1016/j.celrep.2021.109588)
- Kempf, J. et al. 2021. Heterogeneity of neurons reprogrammed from spinal cord astrocytes by the proneural factors Ascl1 and Neurogenin2. Cell Reports 36(3), article number: 109409. (10.1016/j.celrep.2021.109409)
2019
- Petrik, D. and Encinas, J. M. 2019. Perspective: Of mice and men - How widespreads adult neurogenesis?. Frontiers in Neuroscience 13, article number: 923. (10.3389/fnins.2019.00923)
2018
- Petrik, D. et al. 2018. Epithelial sodium channel regulates adult neural stem cell proliferation in a flow-dependent manner. Cell Stem Cell 22(6), pp. 865-878.e8. (10.1016/j.stem.2018.04.016)
2016
- Götz, M., Nakafuku, M. and Petrik, D. 2016. Neurogenesis in the developing and adult brain- similarities and key differences. Cold Spring Harbor Perspectives in Biology 8(7), pp. a018853. (10.1101/cshperspect.a018853)
- Gascón, S. et al. 2016. Identification and successful negotiation of a metabolic checkpoint in direct neuronal reprogramming. Cell Stem Cell 18(3), pp. 396-409. (10.1016/j.stem.2015.12.003)
2015
- Latchney, S. E., Jiang, Y., Petrik, D. P., Eisch, A. J. and Hsieh, J. 2015. Inducible knockout of Mef2a, -c, and -d from nestin-expressing stem/progenitor cells and their progeny unexpectedly uncouples neurogenesis and dendritogenesis in vivo. FASEB Journal 29(12), pp. 5059-5071. (10.1096/fj.15-275651)
- Petrik, D., Latchney, S. E., Masiulis, I., Yun, S., Zhang, Z., Wu, J. I. and Eisch, A. J. 2015. Chromatin remodeling factor Brg1 supports the early maintenance and late responsiveness of nestin-lineage adult neural stem and progenitor cells. Stem Cells 33(12), pp. 3655-3665. (10.1002/stem.2215)
2014
- DeCarolis, N. A. et al. 2014. 56Fe particle exposure results in a long-lasting increase in a cellular index of genomic instability and transiently suppresses adult hippocampal neurogenesis in vivo. Life Sciences in Space Research 2, pp. 70-79. (10.1016/j.lssr.2014.06.004)
2013
- Ninkovic, J. et al. 2013. The BAF complex interacts with Pax6 in adult neural progenitors to establish a neurogenic cross-regulatory transcriptional network. Cell Stem Cell 13(4), pp. 403-418. (10.1016/j.stem.2013.07.002)
- Petrik, D., Yun, S., Latchney, S. E., Kamrudin, S., LeBlanc, J. A., Bibb, J. A. and Eisch, A. J. 2013. Early postnatal in vivo gliogenesis from nestin-lineage progenitors requires Cdk5. PLoS ONE 8(8), article number: e72819. (10.1371/journal.pone.0072819)
- DeCarolis, N. A. et al. 2013. In vivocontribution of nestin- and GLAST-lineage cells to adult hippocampal neurogenesis. Hippocampus 23(8), pp. 708-719. (10.1002/hipo.22130)
2012
- Eisch, A. J. and Petrik, D. 2012. Depression and Hippocampal Neurogenesis: A road to remission?. Science 338(6103), pp. 72-75. (10.1126/science.1222941)
- Petrik, D., Jiang, Y., Birnbaum, S. G., Powell, C. M., Kim, M., Hsieh, J. and Eisch, A. J. 2012. Functional and mechanistic exploration of an adult neurogenesis-promoting small molecule. FASEB Journal 26(8), pp. 3148-3162. (10.1096/fj.11-201426)
- Petrik, D., Lagace, D. C. and Eisch, A. J. 2012. The neurogenesis hypothesis of affective and anxiety disorders: Are we mistaking the scaffolding for the building?. Neuropharmacology 62(1), pp. 21-34. (10.1016/j.neuropharm.2011.09.003)
2011
- Petrik, D., Wang, B. and Brenner, R. 2011. Modulation by the BK accessory β4 subunit of phosphorylation-dependent changes in excitability of dentate gyrus granule neurons. European Journal of Neuroscience 34(5), pp. 695-704. (10.1111/j.1460-9568.2011.07799.x)
2007
- Petrik, D. and Brenner, R. 2007. Regulation of STREX exon large conductance, calcium-activated potassium channels by the β4 accessory subunit. Neuroscience 149(4), pp. 789-803. (10.1016/j.neuroscience.2007.07.066)
- Neprasova, H., Anderova, M., Petrik, D., Vargova, L., Kubinova, S., Chvatal, A. and Sykova, E. 2007. High extracellular K+ evokes changes in voltage-dependent K+ and Na+ currents and volume regulation in astrocytes. Pflügers Archiv European Journal of Physiology 453(6), pp. 839-849. (10.1007/s00424-006-0151-9)
2004
- Anderova, M., Antonova, T., Petrik, D., Neprasová, H., Chvátal, A. and Syková, E. 2004. Voltage-dependent potassium currents in hypertrophied rat astrocytes after a cortical stab wound. Glia 48(4), pp. 311-326. (10.1002/glia.20076)
Erthyglau
- Jörgensen, S. K. M. et al. 2024. An analogue of the Prolactin Releasing Peptide reduces obesity and promotes adult neurogenesis. EMBO Reports 25(1), pp. 351-377. (10.1038/s44319-023-00016-2)
- Petrik, D., Jorgensen, S., Eftychidis, V. and Siebzehnrubl, F. A. 2022. Singular adult neural stem cells do not exist. Cells 11(4), article number: 722. (10.3390/cells11040722)
- Gupta, B. et al. 2021. The transcription factor ZEB1 regulates stem cell self-renewal and cell fate in the adult hippocampus. Cell Reports 36(8), article number: 109588. (10.1016/j.celrep.2021.109588)
- Kempf, J. et al. 2021. Heterogeneity of neurons reprogrammed from spinal cord astrocytes by the proneural factors Ascl1 and Neurogenin2. Cell Reports 36(3), article number: 109409. (10.1016/j.celrep.2021.109409)
- Petrik, D. and Encinas, J. M. 2019. Perspective: Of mice and men - How widespreads adult neurogenesis?. Frontiers in Neuroscience 13, article number: 923. (10.3389/fnins.2019.00923)
- Petrik, D. et al. 2018. Epithelial sodium channel regulates adult neural stem cell proliferation in a flow-dependent manner. Cell Stem Cell 22(6), pp. 865-878.e8. (10.1016/j.stem.2018.04.016)
- Götz, M., Nakafuku, M. and Petrik, D. 2016. Neurogenesis in the developing and adult brain- similarities and key differences. Cold Spring Harbor Perspectives in Biology 8(7), pp. a018853. (10.1101/cshperspect.a018853)
- Gascón, S. et al. 2016. Identification and successful negotiation of a metabolic checkpoint in direct neuronal reprogramming. Cell Stem Cell 18(3), pp. 396-409. (10.1016/j.stem.2015.12.003)
- Latchney, S. E., Jiang, Y., Petrik, D. P., Eisch, A. J. and Hsieh, J. 2015. Inducible knockout of Mef2a, -c, and -d from nestin-expressing stem/progenitor cells and their progeny unexpectedly uncouples neurogenesis and dendritogenesis in vivo. FASEB Journal 29(12), pp. 5059-5071. (10.1096/fj.15-275651)
- Petrik, D., Latchney, S. E., Masiulis, I., Yun, S., Zhang, Z., Wu, J. I. and Eisch, A. J. 2015. Chromatin remodeling factor Brg1 supports the early maintenance and late responsiveness of nestin-lineage adult neural stem and progenitor cells. Stem Cells 33(12), pp. 3655-3665. (10.1002/stem.2215)
- DeCarolis, N. A. et al. 2014. 56Fe particle exposure results in a long-lasting increase in a cellular index of genomic instability and transiently suppresses adult hippocampal neurogenesis in vivo. Life Sciences in Space Research 2, pp. 70-79. (10.1016/j.lssr.2014.06.004)
- Ninkovic, J. et al. 2013. The BAF complex interacts with Pax6 in adult neural progenitors to establish a neurogenic cross-regulatory transcriptional network. Cell Stem Cell 13(4), pp. 403-418. (10.1016/j.stem.2013.07.002)
- Petrik, D., Yun, S., Latchney, S. E., Kamrudin, S., LeBlanc, J. A., Bibb, J. A. and Eisch, A. J. 2013. Early postnatal in vivo gliogenesis from nestin-lineage progenitors requires Cdk5. PLoS ONE 8(8), article number: e72819. (10.1371/journal.pone.0072819)
- DeCarolis, N. A. et al. 2013. In vivocontribution of nestin- and GLAST-lineage cells to adult hippocampal neurogenesis. Hippocampus 23(8), pp. 708-719. (10.1002/hipo.22130)
- Eisch, A. J. and Petrik, D. 2012. Depression and Hippocampal Neurogenesis: A road to remission?. Science 338(6103), pp. 72-75. (10.1126/science.1222941)
- Petrik, D., Jiang, Y., Birnbaum, S. G., Powell, C. M., Kim, M., Hsieh, J. and Eisch, A. J. 2012. Functional and mechanistic exploration of an adult neurogenesis-promoting small molecule. FASEB Journal 26(8), pp. 3148-3162. (10.1096/fj.11-201426)
- Petrik, D., Lagace, D. C. and Eisch, A. J. 2012. The neurogenesis hypothesis of affective and anxiety disorders: Are we mistaking the scaffolding for the building?. Neuropharmacology 62(1), pp. 21-34. (10.1016/j.neuropharm.2011.09.003)
- Petrik, D., Wang, B. and Brenner, R. 2011. Modulation by the BK accessory β4 subunit of phosphorylation-dependent changes in excitability of dentate gyrus granule neurons. European Journal of Neuroscience 34(5), pp. 695-704. (10.1111/j.1460-9568.2011.07799.x)
- Petrik, D. and Brenner, R. 2007. Regulation of STREX exon large conductance, calcium-activated potassium channels by the β4 accessory subunit. Neuroscience 149(4), pp. 789-803. (10.1016/j.neuroscience.2007.07.066)
- Neprasova, H., Anderova, M., Petrik, D., Vargova, L., Kubinova, S., Chvatal, A. and Sykova, E. 2007. High extracellular K+ evokes changes in voltage-dependent K+ and Na+ currents and volume regulation in astrocytes. Pflügers Archiv European Journal of Physiology 453(6), pp. 839-849. (10.1007/s00424-006-0151-9)
- Anderova, M., Antonova, T., Petrik, D., Neprasová, H., Chvátal, A. and Syková, E. 2004. Voltage-dependent potassium currents in hypertrophied rat astrocytes after a cortical stab wound. Glia 48(4), pp. 311-326. (10.1002/glia.20076)
Ymchwil
Rydym yn defnyddio ystod eang o dechnegau i astudio ffactorau genetig, epigenetig, fferyllolegol ac amgylcheddol sy'n rheoleiddio bôn-gelloedd niwral mewn ymennydd mamaliaid oedolion. Gan ddefnyddio imiwnocemeg, diwylliannau celloedd a delweddu treigl amser, rydym yn pennu potensial amlochrog a gwahaniaethu bôn-gelloedd a llinachau clonau celloedd unigol. Rydym yn astudio agweddau mecanistig ar reoleiddio bôn-gelloedd trwy ddelweddu calsiwm a chan yr electroffisioleg patch-clamp ar y cyd â'r dilyniannu RNA un gell. Ein nod yw chwilio am ffactorau newydd sy'n rheoleiddio bioleg bôn-gelloedd ac i ddeall sut mae bôn-gelloedd a'u hepil celloedd yn dylanwadu ar swyddogaethau'r ymennydd a'r corff.
Rheoleiddio bôn-gelloedd trwy ddeiet
Mae niwronau newydd yn cael eu cynhyrchu mewn ymennydd oedolion o fôn-gelloedd niwral mewn rhanbarthau arwahanol o'r enw cilfachau niwrogenig oedolion trwy broses o niwrogenesis oedolion. Mae dau gilfach niwrogenig sefydledig ac un heb ei hastudio'n ddigonol yn yr ymennydd mamalaidd. Mae cilfach niwrogenig yr hippocampws yn arwain at niwronau newydd sy'n hanfodol ar gyfer y cof datganol a rheoli hwyliau. Yn waliau'r ventricle ochrol, mae cilfach niwrogenig sy'n cynhyrchu niwronau anaeddfed, sy'n mudo i'r bwlb olfactory i gymryd rhan mewn swyddogaeth olfactory. Mae'r gilfach a archwiliwyd yn llai yn yr hypothalamws, yn waliau'r trydydd fentrigl ymennydd. Yma, mae celloedd arbenigol o'r enw tanycytes yn gwasanaethu mewn rôl ddeuol fel bôn-gelloedd putative yn ogystal â'r rheoleiddwyr metabolaidd i reoli'r ymddygiad bwydo, pwysau corff a heneiddio. Ein nod yw deall sut mae deiet yn dylanwadu ar allu tanycytes fel bôn-gelloedd niwral a nodi ffactorau genetig newydd sy'n ymateb i ddeiet mewn tanycytes. Y nod strategol yw darganfod genynnau penodol y gellir eu trin mewn tanycytes a'u hepil celloedd i frwydro yn erbyn datblygiad gordewdra a achosir gan ddeiet.
Cyfansoddion gwrth-ordewdra
Mae gennym ddiddordeb mewn pennu mechanims cellog a moleciwlaidd o weithredu cyfansoddion gwrth-ordewdra. Rydym yn ymchwilio i botensial niwrogenig cyfansoddion gwrth-ordewdra newydd a sefydlwyd yn flaenorol. Ein nod yw defnyddio niwrogenesis oedolion a niwronau newydd-anedig fel targed ffarmacolegol o therapïau gwrth-ordewdra cyn-glinigol.
Addysgu
- 'Ffisioleg' BI2331 - system nerfol niwrogyhyrol ac awtonomig
- BI3355 'Datblygiadau mewn Ffisioleg' - ffisioleg arennol
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BI3001 Biowyddorau Prosiectau Blwyddyn Derfynol
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BI4001 Prosiectau Meistr Integredig
Bywgraffiad
I am a senior lecturer of the Biomedicine Division within the School of Biosciences at Cardiff University. My original training lies in ion channel biophysics and physiology, however, over the last decade I have been studying the neural stem cells in the brain.
Between 2000-2003, I conducted my diploma Master’s thesis at the Institute of Experimental Medicine of the Academy of Sciences of Czech Republic in Prague, where I investigated the electrophysiological and morphological properties of reactive astrocytes in a model of mechanical brain injury. To deepen my understanding of ion channel biophysics, I enrolled as a PhD student at The University of Texas Health Science Center in San Antonio, USA. In the laboratory of Dr. Robert Brenner, I studied the effects of alternative splicing, reverse phosphorylation and accessory subunits on molecular kinetics of calcium activated (BK) channels and their role in action potential waveform and firing in the granule cell neurons in the hippocampus.
During my post-doctoral career, I have focused on adult neural stem cells and adult neurogenesis, the process of generating new neurons in the adult brain. In 2008, I have joined the laboratory of Professor Amelia Eisch at the University of Texas Southwestern in Dallas. I have led a research in small molecule screening to characterize one of the most used drugs to upregulate adult neurogenesis in the hippocampus called Isoxazole 9. Also, I have studied the effects of epigenetic (such as Brg1) and genetic (Mef2, Cdk5) factors on the biology of adult neural stem cells. In 2013, I relocated back to Europe as a Marie Curie Fellow of the European Research Council to the laboratory of Professor Magdalena Götz. At the Institute of Stem Cell Research of the Helmholtz Centrum Munich and at Ludwig Maximilian University of Munich, I completed the research arch of my career by showing that adult neural stem cells in the brain are mechano-sensitive thanks to the epithelial sodium channel. Also, I have collaborated on projects that involve so called direct cell reprogramming and new single-cell sequencing methods. In September 2019, I joined the Cardiff University as a senior lecturer. In my laboratory, I focus on the adult neural stem cells in hypothalamus and the role of metabolism and diet on their stemness and biology.
Education
2008 Doctor of Philosophy (Ph.D.) in Physiology: The University of Texas HSCSA, San Antonio, USA. Title of PhD Thesis: "The role of the β4 subunit in phosphorylation of calcium-activated potassium (BK) channels.” Supervisor: Dr. Robert Brenner.
- 2003 Bachelor and Master of Engineering (M. Eng./Ing.): Czech University of Life Sciences, Prague, Czech Republic. Title of Master Diploma Thesis: "Electrophysiological and morphological properties of glial cells in different models of astrogliosis in brain and spinal cord tissues.” Supervisor: Prof. Eva Sykova.
Academic Career and Research Experience
2019- Senior Lecturer and Principal Investigator, School of Biosciences, Cardiff University, UK.
2016-2019 Scientist, Helmholtz Zentrum Munich and Ludwig Maximilian University of Munich, Germany.
2014-2016 Marie Curie Fellow, European Research Council, Helmholtz Zentrum Munich, Germany.
2013-2014 Postdoctoral fellow, Lab of Prof. Magdalena Götz, Helmholtz Zentrum Munich, Germany.
2008-2013 Postdoctoral fellow, Lab of Lab of Prof. Amelia Eisch, The University of Texas Southwestern Medical Center, Dallas, USA.
Honours and Awards
2014 – 2016 Marie Curie International Incoming Fellowship
2005, 2006 Vernon Bishop Award, UTHSCSA, Texas, USA
2003 Award of the Foundation of Vaclav Havel, the President of the Czech Republic
2003 Award for the best young scientists, Czech Physiol. Society of J.E. Purkinje
2003 Annual Award for the best Czech university students, Hlavka Foundation
2003 Travel Award, Fulbright Foundation
Professional Membership
- Society for Neuroscience (from 2004)
Professional Activities
- Journal reviewer (Cell Stem Cell, Stem Cells, Neuron etc.)
- Grant reviewer (BBSRC, MRC, Swiss National Science Foundation etc.)
Anrhydeddau a dyfarniadau
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2014 – 2016 Marie Curie International Incoming Fellowship
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2005, 2006 Vernon Bishop Award, UTHSCSA, Texas, USA
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2003 Award of the Foundation of Vaclav Havel, the President of the Czech Republic
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2003 Award for the best young scientists, Czech Physiol. Society of J.E. Purkinje
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2003 Annual Award for the best Czech university students, Hlavka Foundation
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2003 Travel Award, Fulbright Foundation
Aelodaethau proffesiynol
- Society for Neuroscience (from 2004)
- British Neuroscience Association (from 2022)
Safleoedd academaidd blaenorol
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2019- Senior Lecturer and Principal Investigator, School of Biosciences, Cardiff University, UK.
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2016-2019 Scientist, Helmholtz Zentrum Munich and Ludwig Maximilian University of Munich, Germany.
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2014-2016 Marie Curie Fellow, European Research Council, Helmholtz Zentrum Munich, Germany.
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2013-2014 Postdoctoral fellow, Lab of Prof. Magdalena Götz, Helmholtz Zentrum Munich, Germany.
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2008-2013 Postdoctoral fellow, Lab of Lab of Prof. Amelia Eisch, The University of Texas Southwestern Medical Center, Dallas, USA.
Ymrwymiadau siarad cyhoeddus
Cynhadledd AbCam Niwrogenesis Oedolion 2023 , Dresden, Yr Almaen
Cynhadledd Niwrogenesis Môr Du 2023 , Albena, Bwlgaria
2022 Sefydliad Cajal ar gyfer Niwrowyddoniaeth, Madrid, Sbaen
2021 Sefydliad Achucarro ar gyfer Niwrowyddoniaeth, Bilbao, Sbaen
2019 Prif siaradwr yn symposiwm YSA, Prifysgol Feddygol Fienna, Awstria
2019 Sefydliad Cemeg Organig a Biocemeg, Prague, Gweriniaeth Tsiec
2019 Prifysgol Leipzig, Yr Almaen
2019 Prifysgol Caerdydd, Y Deyrnas Unedig
2018 Sefydliad Achucarro ar gyfer Niwrowyddoniaeth, Bilbao, Sbaen
2018 Sefydliad Biolegol, Prifysgol Masaryk, Brno, Gweriniaeth Tsiec
2018 Prifysgol Leeds, Y Deyrnas Unedig
2018 Symposiwm ABCAM ar Niwrogenesis Oedolion, Dresden, Yr Almaen
2016 Seminar WE Heraeus ar Fecaneg Niwronau, Bad Honnef, Yr Almaen
Cyfarfod EuroGenesis 2016 ar Niwrogenesis Oedolion, Bordeaux, Ffrainc
2015 Symposiwm ABCAM ar Niwrogenesis Oedolion, Dresden, Yr Almaen
Cyfarfod Cymdeithas Niwrowyddoniaeth 2015 , Chicago, UDA
Symposiwm Keystone 2014 ar Niwrogenesis Oedolion, Stockholm, Sweden
Cyfarfod Cymdeithas Niwrowyddoniaeth 2011 , Washington, D.C., UDA, UDA
Pwyllgorau ac adolygu
- GW4 MRC DTP Caerdydd Arweinydd Academaidd Caerdydd
- adolygydd cyfnodolion (bôn-gelloedd, bôn-gelloedd, niwron ac ati)
- Grant adolygydd (BBSRC, MRC, Sefydliad Gwyddoniaeth Cenedlaethol y Swistir ac ati)
- Golygydd Guest (Celloedd)
Meysydd goruchwyliaeth
- Sara Jorgensen (myfyrwraig PhD 2020-2024)
- May Surridge-Smith (Myfyriwr Meistr Ynysig 2023-2024)
Cyn-fyfyrwyr:
- Sarah Robbins (myfyriwr MRes 2020-2021)
- Alena Karnosova (Myfyriwr PhD Ymweld 2021)
- Oliver Rowley (Myfyriwr Meistr Integredig 2021-2022)
- Eleanor Lewis (myfyriwr MRes 2022-2023)
- Aleksandra Hajdrych (Myfyriwr Meistr Integredig 2022-2023)
Goruchwyliaeth gyfredol
Sara Jorgensen
Myfyriwr ymchwil
Ymgysylltu
ArrayThemâu ymchwil
Arbenigeddau
- Celloedd bonyn