![Luciana Gonzaga De Oliveira BA MSc PhD](https://profiles-cardiff.imgix.net/attachments/4080?w=200&h=200&auto=format&crop=faces&fit=crop&q=90")
Professor Luciana Gonzaga De Oliveira
(she/they)
BA MSc PhD
Postdoctoral Research Associate (with Prof Rudolf Allemann)
Overview
In Brazil, at UNICAMP I’ve pioneered genome to molecules and enzymes application by using genome-mining strategies Genome to molecules approaches represents an encouraging avenue to elect and isolate new bioactive targets. We've combined genome sequencing and annotation with modern synthetic biology approaches, in vivo and in vitro recombination to access known and unknown metabolites in the scope of gaining improved knowledge of our biodiversity to develop sustainable livelihoods and bioeconomy. By sequencing actinobacteria endophytic and from soil we were able to elect several gene clusters and enzymes of biotechnological interest and decipher unique structures and biosynthetic pathways involved in the biosynthesis of cyclodepsipeptides and hybrid PKS-NRPS encoded compounds by using MS-Guided Genome Mining.
Publication
2022
- Sachito, D. and Gonzaga De Oliveira, L. 2022. Unveiling the bacterial sesquiterpenome of streptomyces sp. CBMAI 2042 discloses cyclases with versatile performances. Journal of the Brazilian Chemical Society 33 (7), pp.734-742. (10.21577/0103-5053.20220013)
2021
- Merino, G. et al., 2021. Introduction to celebrating Latin American talent in chemistry. RSC Advances 11 (63), pp.40216-40219. (10.1039/D1RA90175C)
2020
- Sigrist, R. et al., 2020. Nonlinear biosynthetic assembly of alpiniamide by a hybrid cis/trans-AT PKS-NRPS. ACS Chemical Biology 15 (4), pp.1067-1077. (10.1021/acschembio.0c00081)
- Sigrist, R. et al., 2020. Mass spectrometry-guided genome mining as a tool to uncover novel natural products. Journal of Visualized Experiments 157 e60825. (10.3791/60825)
- Tormet-González, G. D. et al., 2020. An epoxide hydrolase from endophytic Streptomyces shows unique structural features and wide biocatalytic activity. Acta Crystallographica Section D: Structural Biology 76 (9), pp.868-875. (10.1107/S2059798320010402)
2019
- Gonzaga de Oliveira, L. et al. 2019. Whole-genome sequence of the endophytic Streptomyces sp. strain CBMAI 2042, isolated from Citrus sinensis. Microbiology Resource Announcements 8 (2) e01426-18. (10.1128/MRA.01426-18)
- Paulo, B. S. et al., 2019. Cover picture: new cyclodepsipeptide derivatives revealed by genome mining and molecular networking (27/2019). ChemistrySelect 4 (27), pp.7784-7784. (10.1002/slct.201902252)
- Paulo, B. S. et al., 2019. New cyclodepsipeptide derivatives revealed by genome mining and molecular networking. ChemistrySelect 4 (27), pp.7785-7790. (10.1002/slct.201900922)
2018
- Fioramonte, M. et al., 2018. XPlex: an effective, multiplex cross-linking chemistry for acidic residues. Analytical Chemistry 90 (10), pp.6043-6050. (10.1021/acs.analchem.7b05135)
- Paulo, B. et al., 2018. Gene deletion leads to improved valinomycin production by Streptomyces sp. CBMAI 2042. Journal of the Brazilian Chemical Society 30 (3), pp.673-679. (10.21577/0103-5053.20180207)
- Paulo, B. , Sigrist, R. and Gonzaga De Oliveira, L. 2018. Avanços recentes em biossíntese combinatória de policetídeos: perspectivas e desafios Recent advances in combinatorial biosynthesis of polyketides: perspectives and challenges. Química Nova 42 (1), pp.71-83. (10.21577/0100-4042.20170307)
2016
- Angolini, C. F. F. et al., 2016. Genome mining of endophytic Streptomyces wadayamensis reveals high antibiotic production capability. Journal of the Brazilian Chemical Society 27 (8), pp.1465-1475. (10.5935/0103-5053.20160180)
- Chagas, F. O. et al., 2016. Genome sequence of Streptomyces sp. Strain RTd22, an endophyte of the Mexican sunflower. Genome Announcements 4 (4) e00693-16. (10.1128/genomeA.00693-16)
2015
- Angolini, C. F. F. et al., 2015. Direct protocol for ambient mass dpectrometry imaging on agar culture. Analytical Chemistry 87 (13), pp.6925-6930. (10.1021/acs.analchem.5b01538)
- Cruz, P. L. R. d. et al., 2015. Metabolic screening for PKS and NRPS in endophytic actinobacteria from Citrus reticulata. QUIMICA NOVA 38 (3), pp.333-341. (10.5935/0100-4042.20150010)
- Luhavaya, H. et al., 2015. Enzymology of pyran ring A formation in salinomycin biosynthesis. Angewandte Chemie International Edition 54 (46), pp.13622-13625. (10.1002/anie.201507090)
- Sigrist, R. et al., 2015. Nature-inspired enzymatic cascades to build valuable compounds. Biotechnology Advances 33 (5), pp.394-411. (10.1016/j.biotechadv.2015.03.010)
2014
- Gonzaga De Oliveira, L. et al. 2014. Genome sequence of Streptomyces wadayamensis strain A23, an endophytic actinobacterium from Citrus reticulata. Genome Announcements 2 (4) e00625-14. (10.1128/genomeA.00625-14)
- Luhavaya, H. et al., 2014. Site-specific modification of the anticancer and antituberculosis polyether salinomycin by biosynthetic engineering. ChemBioChem 15 (14), pp.2081-2085. (10.1002/cbic.201402300)
2013
- Gonzaga De Oliveira, L. , Pupo, M. T. and Vieira, P. C. 2013. Explorando produtos naturais microbianos nas fronteiras da química e da biologia Exploring natural microbial products at the frontiers of chemistry and biology. Química Nova 36 (10), pp.1577-1586. (10.1590/S0100-40422013001000015)
2010
- da Cruz, G. F. et al., 2010. Searching for monooxygenases and hydrolases in bacteria from an extreme environment. Applied Microbiology and Biotechnology 87 (1), pp.319-329. (10.1007/s00253-010-2485-7)
- Fernández, L. et al., 2010. An efficient method for mutant library creation in Pichia pastoris useful in directed evolution. Biocatalysis and Biotransformation 28 (2), pp.122-129. (10.3109/10242420903505834)
- Mantovani, S. M. , Gonzaga De Oliveira, L. and Marsaioli, A. J. 2010. Esterase screening using whole cells of Brazilian soil microorganisms. Journal of the Brazilian Chemical Society 21 (8), pp.1484-1489. (10.1590/S0103-50532010000800011)
2009
- Pinheiro, L. , Gonzaga De Oliveira, L. and Marsaioli, A. J. 2009. Assessing the absolute configuration of (7S,8R)-(−)-epoxyjasmone. Journal of Molecular Catalysis B: Enzymatic 60 (3-4), pp.133-137. (10.1016/j.molcatb.2009.04.010)
2008
- Chen, L. S. et al., 2008. 1,2-Octanediol deracemization by stereoinversion using whole cells. Journal of Molecular Catalysis B: Enzymatic 54 (1-2), pp.50-54. (10.1016/j.molcatb.2007.11.022)
- Gonzaga De Oliveira, L. , Dias, L. C. and Rosso, G. B. 2008. Sínteses totais das crocacinas A, C e D: novos antibióticos isolados de Chondromyces crocatus E Chondromyces pediculatuspediculatus Total synthesis of crocacins A, C and D: new antibiotics isolated from Chondromyces crocatus and Chondromyces pediculatus. Química Nova 31 (4), pp.854-871. (10.1590/S0100-40422008000400027)
- Mantovani, S. M. , Gonzaga De Oliveira, L. and Marsaioli, A. J. 2008. Whole cell quick E for epoxide hydrolase screening using fluorescent probes. Journal of Molecular Catalysis B: Enzymatic 52-53 , pp.173-177. (10.1016/j.molcatb.2007.12.013)
2007
- Dias, L. C. , Gonzaga De Oliveira, L. and Meira, P. R. R. 2007. Recent results toward the stereoselective synthesis of biologically active natural products. Pure and Applied Chemistry 79 (2), pp.163-172. (10.1351/pac200779020163)
2006
- Gonzaga De Oliveira, L. et al. 2006. Spirofungins A and B: a reassignment of Kiyota's spiroketals. Tetrahedron Letters 47 (14), pp.2413-2418. (10.1016/j.tetlet.2006.01.153)
2005
- Dias, L. C. et al., 2005. Total synthesis of the potent immunosuppressant (−)-Pironetin. Arkivoc 2005 (6), pp.62-87. (10.3998/ark.5550190.0006.607)
- Dias, L. C. et al., 2005. Total synthesis of (+)-crocacin D. The Journal of Organic Chemistry 70 (6), pp.2225-2234. (10.1021/jo047732k)
2004
- Dias, L. C. and Gonzaga De Oliveira, L. 2004. Short synthesis of the 6,6-spiroketal cores of spirofungins A and B. Organic Letters 6 (15), pp.2587-2590. (10.1021/ol0491078)
- Pérez González, M. et al., 2004. TOPS-MODE based QSARs derived from heterogeneous series of compounds. Applications to the design of new anti-inflammatory compounds. Bioorganic and Medicinal Chemistry 12 (16), pp.4467-4475. (10.1016/j.bmc.2004.05.035)
2003
- Dias, L. C. , Gonzaga De Oliveira, L. and de Sousa, M. A. 2003. Total synthesis of (−)-pironetin. Organic Letters 5 (3), pp.265-268. (10.1021/ol027211o)
2001
- Dias, L. C. and Gonzaga De Oliveira, L. 2001. Total synthesis of (+)-crocacin C. Organic Letters 3 (24), pp.3951-3954. (10.1021/ol016845c)
Articles
- Angolini, C. F. F. et al., 2016. Genome mining of endophytic Streptomyces wadayamensis reveals high antibiotic production capability. Journal of the Brazilian Chemical Society 27 (8), pp.1465-1475. (10.5935/0103-5053.20160180)
- Angolini, C. F. F. et al., 2015. Direct protocol for ambient mass dpectrometry imaging on agar culture. Analytical Chemistry 87 (13), pp.6925-6930. (10.1021/acs.analchem.5b01538)
- Chagas, F. O. et al., 2016. Genome sequence of Streptomyces sp. Strain RTd22, an endophyte of the Mexican sunflower. Genome Announcements 4 (4) e00693-16. (10.1128/genomeA.00693-16)
- Chen, L. S. et al., 2008. 1,2-Octanediol deracemization by stereoinversion using whole cells. Journal of Molecular Catalysis B: Enzymatic 54 (1-2), pp.50-54. (10.1016/j.molcatb.2007.11.022)
- Cruz, P. L. R. d. et al., 2015. Metabolic screening for PKS and NRPS in endophytic actinobacteria from Citrus reticulata. QUIMICA NOVA 38 (3), pp.333-341. (10.5935/0100-4042.20150010)
- da Cruz, G. F. et al., 2010. Searching for monooxygenases and hydrolases in bacteria from an extreme environment. Applied Microbiology and Biotechnology 87 (1), pp.319-329. (10.1007/s00253-010-2485-7)
- Dias, L. C. and Gonzaga De Oliveira, L. 2004. Short synthesis of the 6,6-spiroketal cores of spirofungins A and B. Organic Letters 6 (15), pp.2587-2590. (10.1021/ol0491078)
- Dias, L. C. and Gonzaga De Oliveira, L. 2001. Total synthesis of (+)-crocacin C. Organic Letters 3 (24), pp.3951-3954. (10.1021/ol016845c)
- Dias, L. C. , Gonzaga De Oliveira, L. and de Sousa, M. A. 2003. Total synthesis of (−)-pironetin. Organic Letters 5 (3), pp.265-268. (10.1021/ol027211o)
- Dias, L. C. , Gonzaga De Oliveira, L. and Meira, P. R. R. 2007. Recent results toward the stereoselective synthesis of biologically active natural products. Pure and Applied Chemistry 79 (2), pp.163-172. (10.1351/pac200779020163)
- Dias, L. C. et al., 2005. Total synthesis of the potent immunosuppressant (−)-Pironetin. Arkivoc 2005 (6), pp.62-87. (10.3998/ark.5550190.0006.607)
- Dias, L. C. et al., 2005. Total synthesis of (+)-crocacin D. The Journal of Organic Chemistry 70 (6), pp.2225-2234. (10.1021/jo047732k)
- Fernández, L. et al., 2010. An efficient method for mutant library creation in Pichia pastoris useful in directed evolution. Biocatalysis and Biotransformation 28 (2), pp.122-129. (10.3109/10242420903505834)
- Fioramonte, M. et al., 2018. XPlex: an effective, multiplex cross-linking chemistry for acidic residues. Analytical Chemistry 90 (10), pp.6043-6050. (10.1021/acs.analchem.7b05135)
- Gonzaga De Oliveira, L. , Dias, L. C. and Rosso, G. B. 2008. Sínteses totais das crocacinas A, C e D: novos antibióticos isolados de Chondromyces crocatus E Chondromyces pediculatuspediculatus Total synthesis of crocacins A, C and D: new antibiotics isolated from Chondromyces crocatus and Chondromyces pediculatus. Química Nova 31 (4), pp.854-871. (10.1590/S0100-40422008000400027)
- Gonzaga De Oliveira, L. et al. 2006. Spirofungins A and B: a reassignment of Kiyota's spiroketals. Tetrahedron Letters 47 (14), pp.2413-2418. (10.1016/j.tetlet.2006.01.153)
- Gonzaga De Oliveira, L. , Pupo, M. T. and Vieira, P. C. 2013. Explorando produtos naturais microbianos nas fronteiras da química e da biologia Exploring natural microbial products at the frontiers of chemistry and biology. Química Nova 36 (10), pp.1577-1586. (10.1590/S0100-40422013001000015)
- Gonzaga de Oliveira, L. et al. 2019. Whole-genome sequence of the endophytic Streptomyces sp. strain CBMAI 2042, isolated from Citrus sinensis. Microbiology Resource Announcements 8 (2) e01426-18. (10.1128/MRA.01426-18)
- Gonzaga De Oliveira, L. et al. 2014. Genome sequence of Streptomyces wadayamensis strain A23, an endophytic actinobacterium from Citrus reticulata. Genome Announcements 2 (4) e00625-14. (10.1128/genomeA.00625-14)
- Luhavaya, H. et al., 2015. Enzymology of pyran ring A formation in salinomycin biosynthesis. Angewandte Chemie International Edition 54 (46), pp.13622-13625. (10.1002/anie.201507090)
- Luhavaya, H. et al., 2014. Site-specific modification of the anticancer and antituberculosis polyether salinomycin by biosynthetic engineering. ChemBioChem 15 (14), pp.2081-2085. (10.1002/cbic.201402300)
- Mantovani, S. M. , Gonzaga De Oliveira, L. and Marsaioli, A. J. 2010. Esterase screening using whole cells of Brazilian soil microorganisms. Journal of the Brazilian Chemical Society 21 (8), pp.1484-1489. (10.1590/S0103-50532010000800011)
- Mantovani, S. M. , Gonzaga De Oliveira, L. and Marsaioli, A. J. 2008. Whole cell quick E for epoxide hydrolase screening using fluorescent probes. Journal of Molecular Catalysis B: Enzymatic 52-53 , pp.173-177. (10.1016/j.molcatb.2007.12.013)
- Merino, G. et al., 2021. Introduction to celebrating Latin American talent in chemistry. RSC Advances 11 (63), pp.40216-40219. (10.1039/D1RA90175C)
- Paulo, B. et al., 2018. Gene deletion leads to improved valinomycin production by Streptomyces sp. CBMAI 2042. Journal of the Brazilian Chemical Society 30 (3), pp.673-679. (10.21577/0103-5053.20180207)
- Paulo, B. , Sigrist, R. and Gonzaga De Oliveira, L. 2018. Avanços recentes em biossíntese combinatória de policetídeos: perspectivas e desafios Recent advances in combinatorial biosynthesis of polyketides: perspectives and challenges. Química Nova 42 (1), pp.71-83. (10.21577/0100-4042.20170307)
- Paulo, B. S. et al., 2019. Cover picture: new cyclodepsipeptide derivatives revealed by genome mining and molecular networking (27/2019). ChemistrySelect 4 (27), pp.7784-7784. (10.1002/slct.201902252)
- Paulo, B. S. et al., 2019. New cyclodepsipeptide derivatives revealed by genome mining and molecular networking. ChemistrySelect 4 (27), pp.7785-7790. (10.1002/slct.201900922)
- Pérez González, M. et al., 2004. TOPS-MODE based QSARs derived from heterogeneous series of compounds. Applications to the design of new anti-inflammatory compounds. Bioorganic and Medicinal Chemistry 12 (16), pp.4467-4475. (10.1016/j.bmc.2004.05.035)
- Pinheiro, L. , Gonzaga De Oliveira, L. and Marsaioli, A. J. 2009. Assessing the absolute configuration of (7S,8R)-(−)-epoxyjasmone. Journal of Molecular Catalysis B: Enzymatic 60 (3-4), pp.133-137. (10.1016/j.molcatb.2009.04.010)
- Sachito, D. and Gonzaga De Oliveira, L. 2022. Unveiling the bacterial sesquiterpenome of streptomyces sp. CBMAI 2042 discloses cyclases with versatile performances. Journal of the Brazilian Chemical Society 33 (7), pp.734-742. (10.21577/0103-5053.20220013)
- Sigrist, R. et al., 2015. Nature-inspired enzymatic cascades to build valuable compounds. Biotechnology Advances 33 (5), pp.394-411. (10.1016/j.biotechadv.2015.03.010)
- Sigrist, R. et al., 2020. Nonlinear biosynthetic assembly of alpiniamide by a hybrid cis/trans-AT PKS-NRPS. ACS Chemical Biology 15 (4), pp.1067-1077. (10.1021/acschembio.0c00081)
- Sigrist, R. et al., 2020. Mass spectrometry-guided genome mining as a tool to uncover novel natural products. Journal of Visualized Experiments 157 e60825. (10.3791/60825)
- Tormet-González, G. D. et al., 2020. An epoxide hydrolase from endophytic Streptomyces shows unique structural features and wide biocatalytic activity. Acta Crystallographica Section D: Structural Biology 76 (9), pp.868-875. (10.1107/S2059798320010402)
Biography
Current and Previous Positions
2022- Research Associate, School of Chemistry, Cardiff University
2020-2022 Associate Professor, Department of Organic Chemistry, Institute of Chemistry, UNICAMP (Full-time)
2012-2013 Academic Visitor, University of Cambridge, Department of Biochemistry
Advisor: Prof Peter F. Leadlay, Fellowship: FAPESP (BR), Focus on Biosynthesis and Enzymology
June 2010 Promoted to Assistant Professor MS3-II, Department of Organic Chemistry, Institute of Chemistry, UNICAMP (Full-time)
June/2008 Assistant Professor MS3-I, Department of Organic Chemistry, Institute of Chemistry, UNICAMP (Full-time)
2007-2007 Postdoctoral Fellow, Max Planck Institut für Kohlenforschung
Advisor: Prof. Manfred T. Reetz, Focus on Molecular Biology and Directed Evolution
2005-2008 Postdoctoral Fellow, Department of Organic Chemistry, Institute of Chemistry, UNICAMP, Brazil
Advisor: Prof. Anita Jocelyne Marsaioli, Focus on Biocatalysis using Whole Cells Micoorganisms
2000-2004 PhD in Chemistry, University of Campinas, Brazil
Thesis Title: “Total Synthesis of (+)-Crocacins C and D; Synthesis of the 6,6-Spiroketal Core of Spirofungins A and B”
1998-2000 Master’s Degree in Chemistry, University of Campinas, Brazil
Dissertation Title: Towards the Total Synthesis of the Immunosuppressant Agent Pironetin
1994-1997 Undergraduate in Chemistry, University of Campinas, Brazil
Honours and awards
Fellowships, prizes and awards
2020 – Member of the For Women in Science Community Network – Alumni
2020 – Top Downloaded Article – ChemistrySelect – New Cyclodepsipeptides Derivatives Revealed by Genome Mining and Molecular Networking – Wiley
Jan-Feb 2020 – Academic Visitor – Cardiff University – Prof. Rudolf Allemann – Fellowship: Cardiff Mobility Grant
2019 – Cover Picture ChemistrySelect – New Cyclodepsipeptides Derivatives Revealed by Genome Mining and Molecular Networking
2019 – Cover Picture Química Nova – Recent Advances in Combinatorial Biosynthesis of Polyketides: Perspectives and Challenges
2019 – Selected Manuscript to the JBCS Special Issue – Brazilian Researchers Abroad
2018 – Member of the Lindau Alumni Network
2018 – Selected Manuscript to the JBCS and RSC cross-journal collection to celebrate the UK-BR Year of Science and Innovation.
2015 – Participant of the HUMBOLDT COLLOQUIUM – Research Excellence in a Globalized World: Experiences and Challenges from a Brazilian-German Perspective, Alexander von Humboldt Foundation – nominated by the Max Planck Gesellschaft
2014 – Inside Cover ChemBioChem - A European Journal of Chemical Biology: Site-Specific Modification of the Anticancer and Antituberculosis Polyether Salinomycin by Biosynthetic Engineering
2014 – Scientific Merit, PRP/UNICAMP – XXII Undergraduate Scientific Congress - UNICAMP
2014 – Certificate of Commendation - 2nd Meeting of the Brazilian Proteomics Society, Brazilian Proteomics Society
Feb 2012- Feb 2013 – Academic Visitor / Post-Doctorate – University of Cambridge – Department of Biochemistry – Prof Peter F. Leadlay – Fellowiship: FAPESP (BR)
2009 - Participant of the "59th Meeting of Nobel Laureates (19th Meeting dedicated to Chemistry)" – Nominated by Lindau Council/Brazilian Academy of Science
2008 – Grant “For Women in Science” L’Oreal/ABC/Unesco, Brazilian Edition, Chemical Sciences
Jan-May 2007 – Post-Doctorate – Max-Planck-Institut für Kohlenforschung – Prof. Manfred T. Reetz – Fellowship: FAPESP (BR)
2005-2008 - Postdoctoral Fellow, Department of Organic Chemistry, Institute of Chemistry, UNICAMP, Brazil – Prof. Anita Jocelyne Marsaioli – Fellowship: FAPESP (BR)
Professional memberships
Member of the For Women in Science Community Network – Alumni
Member of the Lindau Alumni Network
Member of the LatinXChem.org initiative
Member of The Brazilian Chemical Society since 1995
Member of The American Society of Pharmacognosy
Contact Details
Research themes
Specialisms
- Biocatalysis and enzyme technology
- Natural products and bioactive compounds
- Metabolomic chemistry
- Genomics