Professor Luciana Gonzaga De Oliveira
(she/they)

BA MSc PhD

Postdoctoral Research Associate (with Prof Rudolf Allemann)

: GonzagaDeOliveiraL@cardiff.ac.uk
: Main Building, Room 1.112, Park Place, Cardiff, CF10 3AT

In Brazil, at UNICAMP I’ve pioneered genome to molecules and enzymes application by using genome-mining strategies Genome to molecules approaches represents an encouraging avenue to elect and isolate new bioactive targets. We've combined genome sequencing and annotation with modern synthetic biology approaches, in vivo and in vitro recombination to access known and unknown metabolites in the scope of gaining improved knowledge of our biodiversity to develop sustainable livelihoods and bioeconomy. By sequencing actinobacteria endophytic and from soil we were able to elect several gene clusters and enzymes of biotechnological interest and decipher unique structures and biosynthetic pathways involved in the biosynthesis of cyclodepsipeptides and hybrid PKS-NRPS encoded compounds by using MS-Guided Genome Mining.

Date
Type

2022

Sachito, D. and Gonzaga De Oliveira, L. 2022. Unveiling the bacterial sesquiterpenome of streptomyces sp. CBMAI 2042 discloses cyclases with versatile performances. Journal of the Brazilian Chemical Society 33(7), pp. 734-742. (10.21577/0103-5053.20220013)

2021

Merino, G. et al. 2021. Introduction to celebrating Latin American talent in chemistry. RSC Advances 11(63), pp. 40216-40219. (10.1039/D1RA90175C)

2020

Tormet-González, G. D., Wilson, C., de Oliveira, G. S., dos Santos, J. C., Gonzaga de Oliveira, L. and Dias, M. V. B. 2020. An epoxide hydrolase from endophytic Streptomyces shows unique structural features and wide biocatalytic activity. Acta Crystallographica Section D: Structural Biology 76(9), pp. 868-875. (10.1107/S2059798320010402)
Sigrist, R., Luhavaya, H., McKinnie, S. M. K., Ferreira da Silva, A., Jurberg, I. D., Moore, B. S. and Gonzaga de Oliveira, L. 2020. Nonlinear biosynthetic assembly of alpiniamide by a hybrid cis/trans-AT PKS-NRPS. ACS Chemical Biology 15(4), pp. 1067-1077. (10.1021/acschembio.0c00081)
Sigrist, R., Paulo, B. S., Angolini, C. F. F. and Gonzaga De Oliveira, L. 2020. Mass spectrometry-guided genome mining as a tool to uncover novel natural products. Journal of Visualized Experiments 157, article number: e60825. (10.3791/60825)

2019

Paulo, B. S., Sigrist, R., Angolini, C. F. F. and Gonzaga De Oliveira, L. 2019. Cover picture: new cyclodepsipeptide derivatives revealed by genome mining and molecular networking (27/2019). ChemistrySelect 4(27), pp. 7784-7784. (10.1002/slct.201902252)
Paulo, B. S., Sigrist, R., Angolini, C. F. F. and Gonzaga De Oliveira, L. 2019. New cyclodepsipeptide derivatives revealed by genome mining and molecular networking. ChemistrySelect 4(27), pp. 7785-7790. (10.1002/slct.201900922)
Gonzaga de Oliveira, L., Sigrist, R., Sachetto Paulo, B., Samborskyy, M. and Stajich, J. E. 2019. Whole-genome sequence of the endophytic Streptomyces sp. strain CBMAI 2042, isolated from Citrus sinensis. Microbiology Resource Announcements 8(2), article number: e01426-18. (10.1128/MRA.01426-18)

2018

Paulo, B., Sigrist, R. and Gonzaga De Oliveira, L. 2018. Avanços recentes em biossíntese combinatória de policetídeos: perspectivas e desafios Recent advances in combinatorial biosynthesis of polyketides: perspectives and challenges. Química Nova 42(1), pp. 71-83. (10.21577/0100-4042.20170307)
Paulo, B., Sigrist, R., Angolini, C., Eberlin, M. and Gonzaga de Oliveira, L. 2018. Gene deletion leads to improved valinomycin production by Streptomyces sp. CBMAI 2042. Journal of the Brazilian Chemical Society 30(3), pp. 673-679. (10.21577/0103-5053.20180207)
Fioramonte, M. et al. 2018. XPlex: an effective, multiplex cross-linking chemistry for acidic residues. Analytical Chemistry 90(10), pp. 6043-6050. (10.1021/acs.analchem.7b05135)

2016

Chagas, F. O. et al. 2016. Genome sequence of Streptomyces sp. Strain RTd22, an endophyte of the Mexican sunflower. Genome Announcements 4(4), article number: e00693-16. (10.1128/genomeA.00693-16)
Angolini, C. F. F. et al. 2016. Genome mining of endophytic Streptomyces wadayamensis reveals high antibiotic production capability. Journal of the Brazilian Chemical Society 27(8), pp. 1465-1475. (10.5935/0103-5053.20160180)

2015

Luhavaya, H., Dias, M. V. B., Williams, S. R., Hong, H., Gonzaga De Oliveira, L. and Leadlay, P. F. 2015. Enzymology of pyran ring A formation in salinomycin biosynthesis. Angewandte Chemie International Edition 54(46), pp. 13622-13625. (10.1002/anie.201507090)
Sigrist, R., Costa, B. Z. d., Marsaioli, A. J. and Gonzaga De Oliveira, L. 2015. Nature-inspired enzymatic cascades to build valuable compounds. Biotechnology Advances 33(5), pp. 394-411. (10.1016/j.biotechadv.2015.03.010)
Angolini, C. F. F., Vendramini, P. H., Araújo, F. D. S., Araújo, W. L., Augusti, R., Eberlin, M. N. and Gonzaga De Oliveira, L. 2015. Direct protocol for ambient mass dpectrometry imaging on agar culture. Analytical Chemistry 87(13), pp. 6925-6930. (10.1021/acs.analchem.5b01538)
Cruz, P. L. R. d. et al. 2015. Metabolic screening for PKS and NRPS in endophytic actinobacteria from Citrus reticulata. QUIMICA NOVA 38(3), pp. 333-341. (10.5935/0100-4042.20150010)

2014

Luhavaya, H., Williams, S. R., Hong, H., Gonzaga De Oliveira, L. and Leadlay, P. F. 2014. Site-specific modification of the anticancer and antituberculosis polyether salinomycin by biosynthetic engineering. ChemBioChem 15(14), pp. 2081-2085. (10.1002/cbic.201402300)
Gonzaga De Oliveira, L., Tormet Gonzalez, G. D., Samborsky, M., Marcon, J., Araujo, W. L. and de Azevedo, J. L. 2014. Genome sequence of Streptomyces wadayamensis strain A23, an endophytic actinobacterium from Citrus reticulata. Genome Announcements 2(4), article number: e00625-14. (10.1128/genomeA.00625-14)

2013

Gonzaga De Oliveira, L., Pupo, M. T. and Vieira, P. C. 2013. Explorando produtos naturais microbianos nas fronteiras da química e da biologia Exploring natural microbial products at the frontiers of chemistry and biology. Química Nova 36(10), pp. 1577-1586. (10.1590/S0100-40422013001000015)

2010

da Cruz, G. F. et al. 2010. Searching for monooxygenases and hydrolases in bacteria from an extreme environment. Applied Microbiology and Biotechnology 87(1), pp. 319-329. (10.1007/s00253-010-2485-7)
Mantovani, S. M., Gonzaga De Oliveira, L. and Marsaioli, A. J. 2010. Esterase screening using whole cells of Brazilian soil microorganisms. Journal of the Brazilian Chemical Society 21(8), pp. 1484-1489. (10.1590/S0103-50532010000800011)
Fernández, L., Jiao, N., Soni, P., Gumulya, Y., Gonzaga De Oliveira, L. and Reetz, M. T. 2010. An efficient method for mutant library creation in Pichia pastoris useful in directed evolution. Biocatalysis and Biotransformation 28(2), pp. 122-129. (10.3109/10242420903505834)

2009

Pinheiro, L., Gonzaga De Oliveira, L. and Marsaioli, A. J. 2009. Assessing the absolute configuration of (7S,8R)-(−)-epoxyjasmone. Journal of Molecular Catalysis B: Enzymatic 60(3-4), pp. 133-137. (10.1016/j.molcatb.2009.04.010)

2008

Chen, L. S., Mantovani, S. M., Gonzaga De Oliveira, L., Duarte, M. C. and Marsaioli, A. J. 2008. 1,2-Octanediol deracemization by stereoinversion using whole cells. Journal of Molecular Catalysis B: Enzymatic 54(1-2), pp. 50-54. (10.1016/j.molcatb.2007.11.022)
Mantovani, S. M., Gonzaga De Oliveira, L. and Marsaioli, A. J. 2008. Whole cell quick E for epoxide hydrolase screening using fluorescent probes. Journal of Molecular Catalysis B: Enzymatic 52-53, pp. 173-177. (10.1016/j.molcatb.2007.12.013)
Gonzaga De Oliveira, L., Dias, L. C. and Rosso, G. B. 2008. Sínteses totais das crocacinas A, C e D: novos antibióticos isolados de Chondromyces crocatus E Chondromyces pediculatuspediculatus Total synthesis of crocacins A, C and D: new antibiotics isolated from Chondromyces crocatus and Chondromyces pediculatus. Química Nova 31(4), pp. 854-871. (10.1590/S0100-40422008000400027)

2007

Dias, L. C., Gonzaga De Oliveira, L. and Meira, P. R. R. 2007. Recent results toward the stereoselective synthesis of biologically active natural products. Pure and Applied Chemistry 79(2), pp. 163-172. (10.1351/pac200779020163)

2006

Gonzaga De Oliveira, L., Dias, L. C., Sakauchi, H. and Kiyota, H. 2006. Spirofungins A and B: a reassignment of Kiyota's spiroketals. Tetrahedron Letters 47(14), pp. 2413-2418. (10.1016/j.tetlet.2006.01.153)

2005

Dias, L. C., Gonzaga De Oliveira, L., Sousa, M. A. d., Ellensohn, R. M. and Krapcho (editor), P. 2005. Total synthesis of the potent immunosuppressant (−)-Pironetin. Arkivoc 2005(6), pp. 62-87. (10.3998/ark.5550190.0006.607)
Dias, L. C., Gonzaga De Oliveira, L., Vilcachagua, J. D. and Nigsch, F. 2005. Total synthesis of (+)-crocacin D. The Journal of Organic Chemistry 70(6), pp. 2225-2234. (10.1021/jo047732k)

2004

Pérez González, M., Dias, L. C., Helguera, A. M., Rodrı́guez, Y. M., Gonzaga De Oliveira, L., Gomez, L. T. and Diaz, H. G. 2004. TOPS-MODE based QSARs derived from heterogeneous series of compounds. Applications to the design of new anti-inflammatory compounds. Bioorganic and Medicinal Chemistry 12(16), pp. 4467-4475. (10.1016/j.bmc.2004.05.035)
Dias, L. C. and Gonzaga De Oliveira, L. 2004. Short synthesis of the 6,6-spiroketal cores of spirofungins A and B. Organic Letters 6(15), pp. 2587-2590. (10.1021/ol0491078)

2003

Dias, L. C., Gonzaga De Oliveira, L. and de Sousa, M. A. 2003. Total synthesis of (−)-pironetin. Organic Letters 5(3), pp. 265-268. (10.1021/ol027211o)

2001

Dias, L. C. and Gonzaga De Oliveira, L. 2001. Total synthesis of (+)-crocacin C. Organic Letters 3(24), pp. 3951-3954. (10.1021/ol016845c)

Current and Previous Positions

2022- Research Associate, School of Chemistry, Cardiff University

2020-2022 Associate Professor, Department of Organic Chemistry, Institute of Chemistry, UNICAMP (Full-time)

2012-2013 Academic Visitor, University of Cambridge, Department of Biochemistry

Advisor: Prof Peter F. Leadlay, Fellowship: FAPESP (BR), Focus on Biosynthesis and Enzymology

June 2010 Promoted to Assistant Professor MS3-II, Department of Organic Chemistry, Institute of Chemistry, UNICAMP (Full-time)

June/2008 Assistant Professor MS3-I, Department of Organic Chemistry, Institute of Chemistry, UNICAMP (Full-time)

2007-2007 Postdoctoral Fellow, Max Planck Institut für Kohlenforschung

Advisor: Prof. Manfred T. Reetz, Focus on Molecular Biology and Directed Evolution

2005-2008 Postdoctoral Fellow, Department of Organic Chemistry, Institute of Chemistry, UNICAMP, Brazil

Advisor: Prof. Anita Jocelyne Marsaioli, Focus on Biocatalysis using Whole Cells Micoorganisms

2000-2004 PhD in Chemistry, University of Campinas, Brazil

Thesis Title: “Total Synthesis of (+)-Crocacins C and D; Synthesis of the 6,6-Spiroketal Core of Spirofungins A and B”

1998-2000 Master’s Degree in Chemistry, University of Campinas, Brazil

Dissertation Title: Towards the Total Synthesis of the Immunosuppressant Agent Pironetin

1994-1997 Undergraduate in Chemistry, University of Campinas, Brazil

Honours and awards

Fellowships, prizes and awards

2020 – Member of the For Women in Science Community Network – Alumni

2020 – Top Downloaded Article – ChemistrySelect – New Cyclodepsipeptides Derivatives Revealed by Genome Mining and Molecular Networking – Wiley

Jan-Feb 2020 – Academic Visitor – Cardiff University – Prof. Rudolf Allemann – Fellowship: Cardiff Mobility Grant

2019 – Cover Picture ChemistrySelect – New Cyclodepsipeptides Derivatives Revealed by Genome Mining and Molecular Networking

2019 – Cover Picture Química Nova – Recent Advances in Combinatorial Biosynthesis of Polyketides: Perspectives and Challenges

2019 – Selected Manuscript to the JBCS Special Issue – Brazilian Researchers Abroad

2018 – Member of the Lindau Alumni Network

2018 – Selected Manuscript to the JBCS and RSC cross-journal collection to celebrate the UK-BR Year of Science and Innovation.

2015 – Participant of the HUMBOLDT COLLOQUIUM – Research Excellence in a Globalized World: Experiences and Challenges from a Brazilian-German Perspective, Alexander von Humboldt Foundation – nominated by the Max Planck Gesellschaft

2014 – Inside Cover ChemBioChem - A European Journal of Chemical Biology: Site-Specific Modification of the Anticancer and Antituberculosis Polyether Salinomycin by Biosynthetic Engineering

2014 – Scientific Merit, PRP/UNICAMP – XXII Undergraduate Scientific Congress - UNICAMP

2014 – Certificate of Commendation - 2nd Meeting of the Brazilian Proteomics Society, Brazilian Proteomics Society

Feb 2012- Feb 2013 – Academic Visitor / Post-Doctorate – University of Cambridge – Department of Biochemistry – Prof Peter F. Leadlay – Fellowiship: FAPESP (BR)

2009 - Participant of the "59th Meeting of Nobel Laureates (19th Meeting dedicated to Chemistry)" – Nominated by Lindau Council/Brazilian Academy of Science

2008 – Grant “For Women in Science” L’Oreal/ABC/Unesco, Brazilian Edition, Chemical Sciences

Jan-May 2007 – Post-Doctorate – Max-Planck-Institut für Kohlenforschung – Prof. Manfred T. Reetz – Fellowship: FAPESP (BR)

2005-2008 - Postdoctoral Fellow, Department of Organic Chemistry, Institute of Chemistry, UNICAMP, Brazil – Prof. Anita Jocelyne Marsaioli – Fellowship: FAPESP (BR)

Professional memberships

Member of the For Women in Science Community Network – Alumni

Member of the Lindau Alumni Network

Member of the LatinXChem.org initiative

Member of The Brazilian Chemical Society since 1995

Member of The American Society of Pharmacognosy

Research themes

Specialisms

Biocatalysis and enzyme technology
Natural products and bioactive compounds
Metabolomic chemistry
Genomics

Professor Luciana Gonzaga De Oliveira (she/they) BA MSc PhD

Overview

Publication