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Dr Helen Brown
(hi)
Darlithydd - Microbioleg (T & R)
Ysgol y Biowyddorau
- BrownH19@caerdydd.ac.uk
- +44 29225 14560
- Adeilad Syr Martin Evans, Ystafell W2/12, Rhodfa'r Amgueddfa, Caerdydd, CF10 3AX
- Ar gael fel goruchwyliwr ôl-raddedig
Trosolwyg
Rwy'n athro mewn Microbioleg. Fy niddordeb ymchwil yw ffurfio bioffilm fel mecanwaith ymwrthedd a pathogenicity. Mae fy ymchwil presennol yn canolbwyntio ar ryngweithio rhwng ffibroblastau a phathogenau manteisgar o fewn heintiau clwyfau cronig. Mae bacteria yn halogi ac yn ffurfio bioffilmiau o fewn clwyfau yn gynnar yn y broses iacháu ac er ein bod yn gwybod bod mwyafrif y clwyfau yn cynnwys bioffilmiau, dim ond is-adran o'r cymunedau hyn sy'n mynd ymlaen i achosi heintiau symptomatig. Rwy'n gobeithio deall sut y gall rhyngweithiadau gwesteiwr-microbaidd a microbaidd ddylanwadu ar wella clwyfau a'r cyfle i heintiau symptomatig ffurfio. Rwy'n canolbwyntio'n bennaf ar bathogenau manteisgar, fel Staphylococcus aureus, sy'n aml yn rhan o'r amgylchedd lleol neu'n cynnal cymunedau microbaidd, ond yn yr amodau cywir gallant achosi heintiau. Cyn dechrau ar fy PhD, gweithiais am chwe blynedd yn y diwydiant fferyllol, gan ddefnyddio profion celloedd mamalaidd i asesu diogelwch a nerth biolegol cyffuriau.
Cyhoeddiad
2023
- Kempf, F. et al. 2023. Inflammatory responses induced by the monophasic variant of salmonella typhimurium in pigs play a role in the high shedder phenotype and fecal microbiota composition. mSystems 8(1), article number: e00852-22. (10.1128/msystems.00852-22)
2022
- Abbott, C., Grout, E., Morris, T. and Brown, H. L. 2022. Cutibacterium acnes biofilm forming clinical isolates modify the formation and structure of Staphylococcus aureus biofilms, increasing their susceptibility to antibiotics. Anaerobe 76, article number: 102580. (10.1016/j.anaerobe.2022.102580)
- Knight, R. et al. 2022. Oral progenitor cell line-derived small extracellular vesicles as a treatment for preferential wound healing outcome. Stem Cells Translational Medicine 11(8), pp. 861-875. (10.1093/stcltm/szac037)
- Brown, H. L. 2022. Microbial growth. In: Rezaei, N. ed. Encyclopedia of Infection and Immunity., Vol. 1. Elsevier, pp. 324-335., (10.1016/B978-0-12-818731-9.00190-7)
2021
- Brown, H., Clayton, A. and Stephens, P. 2021. The role of bacterial extracellular vesicles in chronic wound infections: current knowledge and future challenges. Wound Repair and Regeneration 29(6), pp. 864-880. (10.1111/wrr.12949)
2020
- Brown, H. L. et al. 2020. Antibacterial and anti-virulence activity of manuka honey against genetically diverse Staphylococcus pseudintermedius. Applied and Environmental Microbiology 86, article number: e01768-20. (10.1128/AEM.01768-20)
- Brown, H. L., Passey, J. L., Getino, M., Pursley, I., Basu, P., Horton, D. L. and La Ragione, R. M. 2020. The One Health European Joint Programme (OHEJP), 2018-2022: an exemplary One Health initiative. Journal of Medical Microbiology 69(8), pp. 1037-1039. (10.1099/jmm.0.001228)
2015
- Jenkins, R., Roberts, A. and Brown, H. L. 2015. On the antibacterial effects of manuka honey: mechanistic insights. Research and Reports in Biology 6, pp. 215-224. (10.2147/RRB.S75754)
- Reuter, M., Periago, P. M., Mulholland, F., Brown, H. L. and van Vliet, A. H. M. 2015. A PAS domain-containing regulator controls flagella-flagella interactions in Campylobacter jejuni. Frontiers in Microbiology 6, article number: 770. (10.3389/fmicb.2015.00770)
- Brown, H. L., Hanman, K., Reuter, M., Betts, R. P. and van Vliet, A. H. M. 2015. Campylobacter jejuni biofilms contain extracellular DNA and are sensitive to DNase I treatment. Frontiers in Microbiology 6, article number: 699. (10.3389/fmicb.2015.00699)
- Gerlach, R. G., Brown, H. L., Reuter, M., Hanman, K., Betts, R. P. and van Vliet, A. H. M. 2015. Prevention of biofilm formation and removal of existing biofilms by extracellular DNases of Campylobacter jejuni. PLoS ONE 10(3), article number: e0121680. (10.1371/journal.pone.0121680)
2014
- Brown, H. L., Reuter, M., Salt, L. J., Cross, K. L., Betts, R. P., van Vliet, A. H. M. and Griffiths, M. W. 2014. Chicken juice enhances surface attachment and biofilm formation of Campylobacter jejuni. Applied and Environmental Microbiology 80(22), pp. 7053-7060. (10.1128/AEM.02614-14)
2013
- Marlow, V. L., Stanley-Wall, N. R., MacLean, T., Brown, H. and Kiley, T. B. 2013. Blast a biofilm: a hands-on activity for school children and members of the public. Journal of Microbiology and Biology Education 14(2), pp. 152-254. (10.1128/jmbe.v14i2.563)
- Brown, H. L., van Vliet, A. H. M., Betts, R. P. and Reuter, M. 2013. Tetrazolium reduction allows assessment of biofilm formation byCampylobacter jejuniin a food matrix model. Journal of Applied Microbiology 115(5), pp. 1212-1221. (10.1111/jam.12316)
Adrannau llyfrau
- Brown, H. L. 2022. Microbial growth. In: Rezaei, N. ed. Encyclopedia of Infection and Immunity., Vol. 1. Elsevier, pp. 324-335., (10.1016/B978-0-12-818731-9.00190-7)
Erthyglau
- Kempf, F. et al. 2023. Inflammatory responses induced by the monophasic variant of salmonella typhimurium in pigs play a role in the high shedder phenotype and fecal microbiota composition. mSystems 8(1), article number: e00852-22. (10.1128/msystems.00852-22)
- Abbott, C., Grout, E., Morris, T. and Brown, H. L. 2022. Cutibacterium acnes biofilm forming clinical isolates modify the formation and structure of Staphylococcus aureus biofilms, increasing their susceptibility to antibiotics. Anaerobe 76, article number: 102580. (10.1016/j.anaerobe.2022.102580)
- Knight, R. et al. 2022. Oral progenitor cell line-derived small extracellular vesicles as a treatment for preferential wound healing outcome. Stem Cells Translational Medicine 11(8), pp. 861-875. (10.1093/stcltm/szac037)
- Brown, H., Clayton, A. and Stephens, P. 2021. The role of bacterial extracellular vesicles in chronic wound infections: current knowledge and future challenges. Wound Repair and Regeneration 29(6), pp. 864-880. (10.1111/wrr.12949)
- Brown, H. L. et al. 2020. Antibacterial and anti-virulence activity of manuka honey against genetically diverse Staphylococcus pseudintermedius. Applied and Environmental Microbiology 86, article number: e01768-20. (10.1128/AEM.01768-20)
- Brown, H. L., Passey, J. L., Getino, M., Pursley, I., Basu, P., Horton, D. L. and La Ragione, R. M. 2020. The One Health European Joint Programme (OHEJP), 2018-2022: an exemplary One Health initiative. Journal of Medical Microbiology 69(8), pp. 1037-1039. (10.1099/jmm.0.001228)
- Jenkins, R., Roberts, A. and Brown, H. L. 2015. On the antibacterial effects of manuka honey: mechanistic insights. Research and Reports in Biology 6, pp. 215-224. (10.2147/RRB.S75754)
- Reuter, M., Periago, P. M., Mulholland, F., Brown, H. L. and van Vliet, A. H. M. 2015. A PAS domain-containing regulator controls flagella-flagella interactions in Campylobacter jejuni. Frontiers in Microbiology 6, article number: 770. (10.3389/fmicb.2015.00770)
- Brown, H. L., Hanman, K., Reuter, M., Betts, R. P. and van Vliet, A. H. M. 2015. Campylobacter jejuni biofilms contain extracellular DNA and are sensitive to DNase I treatment. Frontiers in Microbiology 6, article number: 699. (10.3389/fmicb.2015.00699)
- Gerlach, R. G., Brown, H. L., Reuter, M., Hanman, K., Betts, R. P. and van Vliet, A. H. M. 2015. Prevention of biofilm formation and removal of existing biofilms by extracellular DNases of Campylobacter jejuni. PLoS ONE 10(3), article number: e0121680. (10.1371/journal.pone.0121680)
- Brown, H. L., Reuter, M., Salt, L. J., Cross, K. L., Betts, R. P., van Vliet, A. H. M. and Griffiths, M. W. 2014. Chicken juice enhances surface attachment and biofilm formation of Campylobacter jejuni. Applied and Environmental Microbiology 80(22), pp. 7053-7060. (10.1128/AEM.02614-14)
- Marlow, V. L., Stanley-Wall, N. R., MacLean, T., Brown, H. and Kiley, T. B. 2013. Blast a biofilm: a hands-on activity for school children and members of the public. Journal of Microbiology and Biology Education 14(2), pp. 152-254. (10.1128/jmbe.v14i2.563)
- Brown, H. L., van Vliet, A. H. M., Betts, R. P. and Reuter, M. 2013. Tetrazolium reduction allows assessment of biofilm formation byCampylobacter jejuniin a food matrix model. Journal of Applied Microbiology 115(5), pp. 1212-1221. (10.1111/jam.12316)
Ymchwil
Rhyngweithiadau cynnal bacterial:
- Rhyngweithio rhwng ffibroblastau (a'u cydrannau cyfrinachol) a'u dylanwad ar bathogendod Staphylococcus aureus
- Deall sut mae cymunedau bacteriol rhywogaethau cymysg yn ymateb i'r secretome lletyol
Rhyngweithio bacteriol-bacteriol:
- Deall sut y gall y cydadwaith rhwng canmoliaeth a phathogenau opportunistic yrru pathogenicity a dyfalbarhad
- Defnyddio'r gymuned microbiome clodwiw i reoli pathogenau manteisgar.
Ymyriadau therapiwtig newydd ar gyfer clwyfau cronig:
- Defnyddio microdonnau i ysgogi iachâd clwyfau dermol a datrys heintiau clwyfau cronig
- Strategaethau therapiwtig gwrthficrobaidd / gwrthfioffilm newydd gan ddefnyddio'r secretome cynnal
Addysgu
Rwy'n dysgu ar y modiwlau canlynol:
Sgiliau BI1001 ar gyfer Gwyddoniaeth
Organebau BI1003 a'r Amgylchedd
Cysyniadau BI2332 o Glefyd
BI3155 Bioleg heintiau ac Epidemioleg
Rwyf hefyd ar gael i oruchwylio myfyrwyr BSc, MSc ac MRes
Bywgraffiad
2022: Darlithydd mewn Microbioleg, Ysgol y Biowyddorau, Prifysgol Caerdydd
2019 - 2022: Ymchwilydd Ôl-ddoethurol, Ysgol Deintyddiaeth, Prifysgol Caerdydd
2018 - 2019: Ymchwilydd Ôl-ddoethurol, Ysgol Meddygaeth Milfeddygol, Prifysgol Surrey
2016 - 2018: Ymchwilydd Ôl-ddoethurol, Ysgol Deintyddiaeth, Prifysgol Caerdydd
2015 - 2016: Ymchwil Ôl-ddoethurol, Ysgol Gwyddorau Chwaraeon ac Iechyd Caerdydd, Prifysgol Metropolitan Caerdydd
2011 - 2015: Myfyriwr PhD, Institue of Food Research/University of East Anglia
2004 - 2011: Swyddog Arbrofol, Is-adran Bioddiogelwch, Labordai Covance Cyf
Anrhydeddau a dyfarniadau
2013: Gwobr Allgymorth Cymdeithas Microbioleg Gyffredinol
2013: Gwobr Newydd-ddyfodiad Allgymorth Prifysgol East Anglia (CUE EAST)
Aelodaethau proffesiynol
2012 ymlaen - Aelod o'r Gymdeithas Microbioleg
Pwyllgorau ac adolygu
2020 ymlaen: Golygydd Mynediad Microbioleg
Themâu ymchwil
Arbenigeddau
- Gofal iechyd oed
- Bacterioleg feddygol
- Microbioleg meddygol
- Microbioleg