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Dr Helen Brown
(hi/ei)
- Ar gael fel goruchwyliwr ôl-raddedig
Timau a rolau for Helen Brown
Darlithydd - Microbioleg (T & R)
Ysgol y Biowyddorau
Trosolwyg
Mae fy ymchwil yn ymchwilio i sut mae aelodau microbiome commensal fel arfer yn dod yn pathogenau opportunistaidd, gan gyfrannu at glwyfau cronig a chanser y prostad. Mae'r ddau gyflwr yn darparu cilfachau imiwnedd lle mae dyfalbarhad microbaidd, dysbiosis, a llid yn gyrru dilyniant clefyd. Maent yn flaenoriaeth ymchwil oherwydd eu baich byd-eang cynyddol, etioleg gymhleth ac opsiynau triniaeth cyfyngedig. Trwy ddadansoddi rhyngweithiadau microbaidd yn yr amgylcheddau gwahanol ond cysylltiedig hyn, rwy'n anelu at ddatgelu mecanweithiau sy'n dylanwadu ar heneiddio ac iechyd. Gan bontio microbioleg, bioleg heintiau, a therapiwteg, rwy'n cydweithio ar draws disgyblaethau i ddatblygu strategaethau arloesol, sy'n canolbwyntio ar y claf ar gyfer modiwleiddio microbiom ac atal clefydau. Mae gen i dri phrif faes ymchwil cydgysylltiedig:
Mae C. acnes yn aelod arferol a niferus o'r microbiom croen, sy'n dominyddu'r amgylchedd anaerobig o fewn chwarennau sebaceous. Fodd bynnag, mae ganddo rôl hefyd mewn heintiau sy'n gysylltiedig â mewnblaniadau ac mae wedi'i nodi mewn biopsiau prostad dynion â chanser y prostad. Mae'r amgylchedd canser yn anoxig, gan ddarparu amgylchedd addas ar gyfer gwladychu C. acnes . Mae'n hysbys hefyd bod gwladychu microbaidd cilfachau y tu allan i'r microbiom arferol yn arwain at fwy o lid, yn yr amgylchedd tiwmor gall y llid hwn yrru dilyniant canser ymhellach.
Gwladychu microbaidd clwyfau cronig: Mae clwyfau cronig yn cael eu gwladychu'n gyflym gan sawl rhywogaeth ficrobaidd a geir yn gyffredin yn yr amgylchedd lleol (h.y. pridd a dŵr) a/neu ficrobiom dynol. Er ei bod yn hysbys bod y mwyafrif o glwyfau cronig yn cael eu gwladychu gyda sawl rhywogaeth, dim ond cyfran fach o glwyfau fydd yn cael eu gwladychu i'r graddau y maent yn cael eu hystyried yn glinigol "heintiedig". Gan ddefnyddio modelau bioffilm aml-rywogaeth a modelau cyd-ddiwylliant (celloedd bacteriol-mamaliaid), rydym yn anelu at archwilio sut mae rhywogaethau yn integreiddio i gymunedau clwyfau presennol a'r ffactorau sy'n gyrru gordyfiant sy'n arwain at haint symptomatig.
Triniaeth probiotig safleoedd clwyfau heintiedig: Mae triniaethau gwrthfiotig safonol yn effeithiol gwael yn erbyn heintiau clwyfau cronig a ddiagnosiwyd yn glinigol, oherwydd ymwrthedd cynhenid llawer o rywogaethau o fewn y clwyf a'u ffordd o fyw bioffilm. Fel y cyfryw, mae angen strategaethau triniaeth newydd ar frys, gyda chynhyrchion probiotig a bwydydd wedi'u eplesu yn darparu ffynhonnell gyfoethog o gynhyrchion gwrthficrobaidd amgen. Rydym yn archwilio potensial gwrth-bioffilm a gwrthficrobaidd bwydydd wedi'u eplesu, porbitoics a chynhyrchion naturiol ar gyfer trin bioffilmiau clwyfau cymhleth.
Cyhoeddiad
2025
- Miles, A., Porch, A., Choi, H., Cripps, S., Brown, H. and Williams, C. F. 2025. Direct measurement of non-thermal microwave effects on bacterial growth and redox dynamics using a novel high-throughput waveguide applicator. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 383(2297), article number: 20240073. (10.1098/rsta.2024.0073)
- Metris, A. et al. 2025. Assessing the safety of microbiome perturbations. Microbial Genomics 11(5), article number: 1405. (10.1099/mgen.0.001405)
2024
- Brown, H. L., Pursley, I. G., Horton, D. L. and La Ragione, R. M. 2024. One health: a structured review and commentary on trends and themes. One Health Outlook 6(1), article number: 17. (10.1186/s42522-024-00111-x)
2023
- Kempf, F. et al. 2023. Inflammatory responses induced by the monophasic variant of salmonella typhimurium in pigs play a role in the high shedder phenotype and fecal microbiota composition. mSystems 8(1), article number: e00852-22. (10.1128/msystems.00852-22)
2022
- Knight, R. et al. 2022. Oral progenitor cell line-derived small extracellular vesicles as a treatment for preferential wound healing outcome. Stem Cells Translational Medicine 11(8), pp. 861-875. (10.1093/stcltm/szac037)
- Abbott, C., Grout, E., Morris, T. and Brown, H. L. 2022. Cutibacterium acnes biofilm forming clinical isolates modify the formation and structure of Staphylococcus aureus biofilms, increasing their susceptibility to antibiotics. Anaerobe 76, article number: 102580. (10.1016/j.anaerobe.2022.102580)
- Brown, H. L. 2022. Microbial growth. In: Rezaei, N. ed. Encyclopedia of Infection and Immunity., Vol. 1. Elsevier, pp. 324-335., (10.1016/B978-0-12-818731-9.00190-7)
2021
- Brown, H., Clayton, A. and Stephens, P. 2021. The role of bacterial extracellular vesicles in chronic wound infections: current knowledge and future challenges. Wound Repair and Regeneration 29(6), pp. 864-880. (10.1111/wrr.12949)
2020
- Brown, H. L. et al. 2020. Antibacterial and anti-virulence activity of manuka honey against genetically diverse Staphylococcus pseudintermedius. Applied and Environmental Microbiology 86, article number: e01768-20. (10.1128/AEM.01768-20)
- Brown, H. L., Passey, J. L., Getino, M., Pursley, I., Basu, P., Horton, D. L. and La Ragione, R. M. 2020. The One Health European Joint Programme (OHEJP), 2018-2022: an exemplary One Health initiative. Journal of Medical Microbiology 69(8), pp. 1037-1039. (10.1099/jmm.0.001228)
2015
- Jenkins, R., Roberts, A. and Brown, H. L. 2015. On the antibacterial effects of manuka honey: mechanistic insights. Research and Reports in Biology 6, pp. 215-224. (10.2147/RRB.S75754)
- Reuter, M., Periago, P. M., Mulholland, F., Brown, H. L. and van Vliet, A. H. M. 2015. A PAS domain-containing regulator controls flagella-flagella interactions in Campylobacter jejuni. Frontiers in Microbiology 6, article number: 770. (10.3389/fmicb.2015.00770)
- Brown, H. L., Hanman, K., Reuter, M., Betts, R. P. and van Vliet, A. H. M. 2015. Campylobacter jejuni biofilms contain extracellular DNA and are sensitive to DNase I treatment. Frontiers in Microbiology 6, article number: 699. (10.3389/fmicb.2015.00699)
- Gerlach, R. G., Brown, H. L., Reuter, M., Hanman, K., Betts, R. P. and van Vliet, A. H. M. 2015. Prevention of biofilm formation and removal of existing biofilms by extracellular DNases of Campylobacter jejuni. PLoS ONE 10(3), article number: e0121680. (10.1371/journal.pone.0121680)
2014
- Brown, H. L., Reuter, M., Salt, L. J., Cross, K. L., Betts, R. P., van Vliet, A. H. M. and Griffiths, M. W. 2014. Chicken juice enhances surface attachment and biofilm formation of Campylobacter jejuni. Applied and Environmental Microbiology 80(22), pp. 7053-7060. (10.1128/AEM.02614-14)
2013
- Marlow, V. L., Stanley-Wall, N. R., MacLean, T., Brown, H. and Kiley, T. B. 2013. Blast a biofilm: a hands-on activity for school children and members of the public. Journal of Microbiology and Biology Education 14(2), pp. 152-254. (10.1128/jmbe.v14i2.563)
- Brown, H. L., van Vliet, A. H. M., Betts, R. P. and Reuter, M. 2013. Tetrazolium reduction allows assessment of biofilm formation byCampylobacter jejuniin a food matrix model. Journal of Applied Microbiology 115(5), pp. 1212-1221. (10.1111/jam.12316)
Adrannau llyfrau
- Brown, H. L. 2022. Microbial growth. In: Rezaei, N. ed. Encyclopedia of Infection and Immunity., Vol. 1. Elsevier, pp. 324-335., (10.1016/B978-0-12-818731-9.00190-7)
Erthyglau
- Miles, A., Porch, A., Choi, H., Cripps, S., Brown, H. and Williams, C. F. 2025. Direct measurement of non-thermal microwave effects on bacterial growth and redox dynamics using a novel high-throughput waveguide applicator. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 383(2297), article number: 20240073. (10.1098/rsta.2024.0073)
- Metris, A. et al. 2025. Assessing the safety of microbiome perturbations. Microbial Genomics 11(5), article number: 1405. (10.1099/mgen.0.001405)
- Brown, H. L., Pursley, I. G., Horton, D. L. and La Ragione, R. M. 2024. One health: a structured review and commentary on trends and themes. One Health Outlook 6(1), article number: 17. (10.1186/s42522-024-00111-x)
- Kempf, F. et al. 2023. Inflammatory responses induced by the monophasic variant of salmonella typhimurium in pigs play a role in the high shedder phenotype and fecal microbiota composition. mSystems 8(1), article number: e00852-22. (10.1128/msystems.00852-22)
- Knight, R. et al. 2022. Oral progenitor cell line-derived small extracellular vesicles as a treatment for preferential wound healing outcome. Stem Cells Translational Medicine 11(8), pp. 861-875. (10.1093/stcltm/szac037)
- Abbott, C., Grout, E., Morris, T. and Brown, H. L. 2022. Cutibacterium acnes biofilm forming clinical isolates modify the formation and structure of Staphylococcus aureus biofilms, increasing their susceptibility to antibiotics. Anaerobe 76, article number: 102580. (10.1016/j.anaerobe.2022.102580)
- Brown, H., Clayton, A. and Stephens, P. 2021. The role of bacterial extracellular vesicles in chronic wound infections: current knowledge and future challenges. Wound Repair and Regeneration 29(6), pp. 864-880. (10.1111/wrr.12949)
- Brown, H. L. et al. 2020. Antibacterial and anti-virulence activity of manuka honey against genetically diverse Staphylococcus pseudintermedius. Applied and Environmental Microbiology 86, article number: e01768-20. (10.1128/AEM.01768-20)
- Brown, H. L., Passey, J. L., Getino, M., Pursley, I., Basu, P., Horton, D. L. and La Ragione, R. M. 2020. The One Health European Joint Programme (OHEJP), 2018-2022: an exemplary One Health initiative. Journal of Medical Microbiology 69(8), pp. 1037-1039. (10.1099/jmm.0.001228)
- Jenkins, R., Roberts, A. and Brown, H. L. 2015. On the antibacterial effects of manuka honey: mechanistic insights. Research and Reports in Biology 6, pp. 215-224. (10.2147/RRB.S75754)
- Reuter, M., Periago, P. M., Mulholland, F., Brown, H. L. and van Vliet, A. H. M. 2015. A PAS domain-containing regulator controls flagella-flagella interactions in Campylobacter jejuni. Frontiers in Microbiology 6, article number: 770. (10.3389/fmicb.2015.00770)
- Brown, H. L., Hanman, K., Reuter, M., Betts, R. P. and van Vliet, A. H. M. 2015. Campylobacter jejuni biofilms contain extracellular DNA and are sensitive to DNase I treatment. Frontiers in Microbiology 6, article number: 699. (10.3389/fmicb.2015.00699)
- Gerlach, R. G., Brown, H. L., Reuter, M., Hanman, K., Betts, R. P. and van Vliet, A. H. M. 2015. Prevention of biofilm formation and removal of existing biofilms by extracellular DNases of Campylobacter jejuni. PLoS ONE 10(3), article number: e0121680. (10.1371/journal.pone.0121680)
- Brown, H. L., Reuter, M., Salt, L. J., Cross, K. L., Betts, R. P., van Vliet, A. H. M. and Griffiths, M. W. 2014. Chicken juice enhances surface attachment and biofilm formation of Campylobacter jejuni. Applied and Environmental Microbiology 80(22), pp. 7053-7060. (10.1128/AEM.02614-14)
- Marlow, V. L., Stanley-Wall, N. R., MacLean, T., Brown, H. and Kiley, T. B. 2013. Blast a biofilm: a hands-on activity for school children and members of the public. Journal of Microbiology and Biology Education 14(2), pp. 152-254. (10.1128/jmbe.v14i2.563)
- Brown, H. L., van Vliet, A. H. M., Betts, R. P. and Reuter, M. 2013. Tetrazolium reduction allows assessment of biofilm formation byCampylobacter jejuniin a food matrix model. Journal of Applied Microbiology 115(5), pp. 1212-1221. (10.1111/jam.12316)
Ymchwil
Rhyngweithiadau cynnal bacterial:
- Rhyngweithio rhwng ffibroblastau (a'u cydrannau cyfrinachol) a'u dylanwad ar bathogendod Staphylococcus aureus
- Deall sut mae cymunedau bacteriol rhywogaethau cymysg yn ymateb i'r secretome lletyol
Rhyngweithio bacteriol-bacteriol:
- Deall sut y gall y cydadwaith rhwng canmoliaeth a phathogenau opportunistic yrru pathogenicity a dyfalbarhad
- Defnyddio'r gymuned microbiome clodwiw i reoli pathogenau manteisgar.
Ymyriadau therapiwtig newydd ar gyfer clwyfau cronig:
- Defnyddio microdonnau i ysgogi iachâd clwyfau dermol a datrys heintiau clwyfau cronig
- Strategaethau therapiwtig gwrthficrobaidd / gwrthfioffilm newydd gan ddefnyddio'r secretome cynnal
Addysgu
Rwy'n dysgu ar y modiwlau canlynol:
Sgiliau BI1001 ar gyfer Gwyddoniaeth
Organebau BI1003 a'r Amgylchedd
Cysyniadau BI2332 o Glefyd
BI3155 Bioleg heintiau ac Epidemioleg
Rwyf hefyd ar gael i oruchwylio myfyrwyr BSc, MSc ac MRes
Bywgraffiad
2022: Darlithydd mewn Microbioleg, Ysgol y Biowyddorau, Prifysgol Caerdydd
2019 - 2022: Ymchwilydd Ôl-ddoethurol, Ysgol Deintyddiaeth, Prifysgol Caerdydd
2018 - 2019: Ymchwilydd Ôl-ddoethurol, Ysgol Meddygaeth Milfeddygol, Prifysgol Surrey
2016 - 2018: Ymchwilydd Ôl-ddoethurol, Ysgol Deintyddiaeth, Prifysgol Caerdydd
2015 - 2016: Ymchwil Ôl-ddoethurol, Ysgol Gwyddorau Chwaraeon ac Iechyd Caerdydd, Prifysgol Metropolitan Caerdydd
2011 - 2015: Myfyriwr PhD, Institue of Food Research/University of East Anglia
2004 - 2011: Swyddog Arbrofol, Is-adran Bioddiogelwch, Labordai Covance Cyf
Anrhydeddau a dyfarniadau
2013: Gwobr Allgymorth Cymdeithas Microbioleg Gyffredinol
2013: Gwobr Newydd-ddyfodiad Allgymorth Prifysgol East Anglia (CUE EAST)
Aelodaethau proffesiynol
2012 ymlaen - Aelod o'r Gymdeithas Microbioleg
Pwyllgorau ac adolygu
2020 ymlaen: Golygydd Mynediad Microbioleg
Contact Details
+44 29225 14560
Adeilad Syr Martin Evans, Ystafell W2/03, Rhodfa'r Amgueddfa, Caerdydd, CF10 3AX
Themâu ymchwil
Arbenigeddau
- Gofal iechyd oed
- Bacterioleg feddygol
- Microbioleg meddygol
- Microbioleg