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Dr Nick Kent
Darllenydd
Ysgol y Biowyddorau
- Ar gael fel goruchwyliwr ôl-raddedig
Trosolwyg
Nid yw DNA mewn celloedd yn gweithredu fel helics dwbl noeth ond mae'n rhwym i wahanol broteinau strwythurol i ffurfio cromatotin. Mae modiwleiddio strwythur cromatin yn broses allweddol ym maes catalysis a rheoleiddio trawsgrifio genynnau, dyblygu DNA ac atgyweirio DNA. Rwy'n defnyddio technegau dilyniannu cromatotin cenhedlaeth nesaf i ddadansoddi swyddogaeth cromatotin mewn amrywiaeth o organebau gan gynnwys bodau dynol, planhigion, pryfed, burum ac archaea. Nod fy ymchwil yw deall bioleg cromatotin sylfaenol sy'n berthnasol i iechyd dynol, gwella cnydau ac esblygiad strwythur genom. Mae fy nghyllid diweddaraf wedi dod o'r BBSRC.
Rolau
–Arweinydd Modiwl: genynnau BI3254 Genynnau i Genomau
–Goruchwyliwr Diogelu Ymbelydredd Ysgol
Arweinydd Canolfan Ymchwil Genomeg
–Cydlynydd Blwyddyn 3
Oes gennych chi ddiddordeb mewn ymuno â'm labordy fel myfyriwr ôl-raddedig hunan-ariannu neu bostdoc/cymrodyr? Cysylltwch â mi drwy e-bost.
Cyhoeddiad
2023
- Ofer, S. et al. 2023. DNA-bridging by an archaeal histone variant via a unique tetramerisation interface. Communications Biology 6(1), article number: 968. (10.1038/s42003-023-05348-2)
- Baldwin, A. et al. 2023. Storage of halved strawberry fruits affects aroma, phytochemical content and gene expression, and is affected by pre-harvest factors. Frontiers in Plant Science 14, article number: 1165056. (10.3389/fpls.2023.1165056)
2019
- Harwood, J. C., Kent, N. A., Allen, N. D. and Harwood, A. J. 2019. Nucleosome dynamics of human iPSC during neural differentiation. EMBO reports 20(6), article number: e46960. (10.15252/embr.201846960)
- Lear, B. et al. 2019. De novo transcriptome analysis of peduncle necking in cut Rosa hybrida cultivar 'H30'. Acta Horticulturae 1263, pp. 351-358.
2017
- Pass, D. A., Sornay, E., Marchbank, A., Crawford, M. R., Paszkiewicz, K., Kent, N. A. and Murray, J. A. H. 2017. Genome-wide chromatin mapping with size resolution reveals a dynamic sub-nucleosomal landscape in Arabidopsis. PLoS Genetics 13(9), article number: e1006988. (10.1371/journal.pgen.1006988)
- Platt, J. L., Kent, N. A., Kimmel, A. R. and Harwood, A. J. 2017. Regulation of nucleosome positioning by a CHD Type III chromatin remodeler and its relationship to developmental gene expression in Dictyostelium. Genome Research 27, pp. 591-600. (10.1101/gr.216309.116)
2016
- Gal, C. et al. 2016. Abo1, a conserved bromodomain AAA-ATPase, maintains global nucleosome occupancy and organisation. EMBO reports 17(1), pp. 79-93. (10.15252/embr.201540476)
2015
- Gal, C., Moore, K. M., Paszkiewicz, K., Kent, N. A. and Whitehall, S. K. 2015. The impact of the HIRA histone chaperone upon global nucleosome architecture. Cell Cycle 14(1), pp. 123-134. (10.4161/15384101.2014.967123)
2013
- Maruyama, H. et al. 2013. An alternative beads-on-a-string chromatin architecture in Thermococcus kodakarensis. Embo Reports 14(8), pp. 711-717. (10.1038/embor.2013.94)
2012
- Chambers, A. L., Ormerod, G., Durley, S. C., Sing, T. L., Brown, G. W., Kent, N. A. and Downs, J. A. 2012. The INO80 chromatin remodeling complex prevents polyploidy and maintains normal chromatin structure at centromeres. Genes & Development 26(23), pp. 2590-2603. (10.1101/gad.199976.112)
- Durand-Dubief, M. et al. 2012. SWI/SNF-like chromatin remodeling factor Fun30 supports point centromere function in S. cerevisiae. PLoS Genetics 8(9), article number: e1002974. (10.1371/journal.pgen.1002974)
- Chambers, A. L., Brownlee, P. M., Durley, S. C., Beacham, T., Kent, N. A. and Downs, J. A. 2012. The two different isoforms of the RSC chromatin remodeling complex play distinct roles in DNA damage responses. PLoS ONE 7(2), article number: e32016. (10.1371/journal.pone.0032016)
2010
- Kent, N. A., Adams, S., Moorhouse, A. and Paszkiewicz, K. 2010. Chromatin particle spectrum analysis: A method for comparative chromatin structure analysis using paired-end mode next-generation DNA sequencing. Nucleic Acids Research 39(5), article number: e26. (10.1093/nar/gkq1183)
2009
- Kent, N. A. 2009. British yeast group meeting. Biochemist e-volution 31(3), pp. 52-53.
- Loney, E. R., Inglis, P. W., Sharp, S., Pryde, F. E., Kent, N. A., Mellor, J. and Louis, E. J. 2009. Repressive and non-repressive chromatin at native telomeres in Saccharomyces cerevisiae. Epigenetics & Chromatin(2), article number: 18. (10.1186/1756-8935-2-18)
2007
- Jones, H. S., Kawauchi, J., Braglia, P., Alen, C. M., Kent, N. A. and Proudfoot, N. J. 2007. RNA polymerase I in yeast transcribes dynamic nucleosomal rDNA. Nature Structural and Molecular Biology Vol 14(2), pp. 123-130. (10.1038/nsmb1199)
- Kent, N. A., Chambers, A. L. and Downs, J. A. 2007. Dual chromatin remodeling roles for RSC during DNA double strand break induction and repair at the yeast MAT locus. Journal of Biological Chemistry 282(38), pp. 27693-27701. (10.1074/jbc.M704707200)
- Sherriff, J. A., Kent, N. A. and Mellor, J. 2007. The Isw2 chromatin-remodeling ATPase cooperates with the Fkh2 transcription factor To repress transcription of the B-type cyclin Gene CLB2. Molecular and Cellular Biology 27(8), pp. 2848-2860. (10.1128/MCB.01798-06)
2004
- Kent, N. A., Eibert, S. M. and Mellor, J. 2004. Cbf1p Is Required for Chromatin Remodeling at Promotor - proximal CACGTG motifs in yeast. The Journal of Biological Chemistry 279(26), pp. 27116 - 27123. (10.1074/jbc.M403818200)
- Ferreiro, A., Powell, N. G., Karabetsou, N., Kent, N. A., Mellor, J. and Waters, R. 2004. Cbf1p modulates chromatin structure, transcription and repair at the Saccharomyces cerevisiae MET16 locus. Nucleic Acids Research 32(5), pp. 1617-1626. (10.1093/nar/gkh324)
- Martinez-Campa, C., Politis, P., Moreau, J., Kent, N. A., Goodall, J., Mellor, J. and Goding, C. R. 2004. Precise nucleosome positioning and the TATA box dictate requirements for the histone H4 tail and the bromodomain factor Bdf1. Molecular Cell 15(1), pp. 69-81. (10.1016/j.molcel.2004.05.022)
2002
- Alen, C., Kent, N. A., Jones, H. S., O'Sullivan, J., Aranda, A. and Proudfoot, N. J. 2002. A role for chromatin remodeling in transcriptional termination by RNA polymerase II. Molecular Cell 10(6), pp. 1441-1452. (10.1016/S1097-2765(02)00778-5)
2001
- Kent, N. A., Karabetsou, N., Politis, P. K. and Mellor, J. 2001. In vivo chromatin remodeling by yeast ISWI homologs Isw1p and Isw2p. Genes and Development 15, pp. 619-626. (10.1101/gad.190301)
Articles
- Ofer, S. et al. 2023. DNA-bridging by an archaeal histone variant via a unique tetramerisation interface. Communications Biology 6(1), article number: 968. (10.1038/s42003-023-05348-2)
- Baldwin, A. et al. 2023. Storage of halved strawberry fruits affects aroma, phytochemical content and gene expression, and is affected by pre-harvest factors. Frontiers in Plant Science 14, article number: 1165056. (10.3389/fpls.2023.1165056)
- Harwood, J. C., Kent, N. A., Allen, N. D. and Harwood, A. J. 2019. Nucleosome dynamics of human iPSC during neural differentiation. EMBO reports 20(6), article number: e46960. (10.15252/embr.201846960)
- Lear, B. et al. 2019. De novo transcriptome analysis of peduncle necking in cut Rosa hybrida cultivar 'H30'. Acta Horticulturae 1263, pp. 351-358.
- Pass, D. A., Sornay, E., Marchbank, A., Crawford, M. R., Paszkiewicz, K., Kent, N. A. and Murray, J. A. H. 2017. Genome-wide chromatin mapping with size resolution reveals a dynamic sub-nucleosomal landscape in Arabidopsis. PLoS Genetics 13(9), article number: e1006988. (10.1371/journal.pgen.1006988)
- Platt, J. L., Kent, N. A., Kimmel, A. R. and Harwood, A. J. 2017. Regulation of nucleosome positioning by a CHD Type III chromatin remodeler and its relationship to developmental gene expression in Dictyostelium. Genome Research 27, pp. 591-600. (10.1101/gr.216309.116)
- Gal, C. et al. 2016. Abo1, a conserved bromodomain AAA-ATPase, maintains global nucleosome occupancy and organisation. EMBO reports 17(1), pp. 79-93. (10.15252/embr.201540476)
- Gal, C., Moore, K. M., Paszkiewicz, K., Kent, N. A. and Whitehall, S. K. 2015. The impact of the HIRA histone chaperone upon global nucleosome architecture. Cell Cycle 14(1), pp. 123-134. (10.4161/15384101.2014.967123)
- Maruyama, H. et al. 2013. An alternative beads-on-a-string chromatin architecture in Thermococcus kodakarensis. Embo Reports 14(8), pp. 711-717. (10.1038/embor.2013.94)
- Chambers, A. L., Ormerod, G., Durley, S. C., Sing, T. L., Brown, G. W., Kent, N. A. and Downs, J. A. 2012. The INO80 chromatin remodeling complex prevents polyploidy and maintains normal chromatin structure at centromeres. Genes & Development 26(23), pp. 2590-2603. (10.1101/gad.199976.112)
- Durand-Dubief, M. et al. 2012. SWI/SNF-like chromatin remodeling factor Fun30 supports point centromere function in S. cerevisiae. PLoS Genetics 8(9), article number: e1002974. (10.1371/journal.pgen.1002974)
- Chambers, A. L., Brownlee, P. M., Durley, S. C., Beacham, T., Kent, N. A. and Downs, J. A. 2012. The two different isoforms of the RSC chromatin remodeling complex play distinct roles in DNA damage responses. PLoS ONE 7(2), article number: e32016. (10.1371/journal.pone.0032016)
- Kent, N. A., Adams, S., Moorhouse, A. and Paszkiewicz, K. 2010. Chromatin particle spectrum analysis: A method for comparative chromatin structure analysis using paired-end mode next-generation DNA sequencing. Nucleic Acids Research 39(5), article number: e26. (10.1093/nar/gkq1183)
- Kent, N. A. 2009. British yeast group meeting. Biochemist e-volution 31(3), pp. 52-53.
- Loney, E. R., Inglis, P. W., Sharp, S., Pryde, F. E., Kent, N. A., Mellor, J. and Louis, E. J. 2009. Repressive and non-repressive chromatin at native telomeres in Saccharomyces cerevisiae. Epigenetics & Chromatin(2), article number: 18. (10.1186/1756-8935-2-18)
- Jones, H. S., Kawauchi, J., Braglia, P., Alen, C. M., Kent, N. A. and Proudfoot, N. J. 2007. RNA polymerase I in yeast transcribes dynamic nucleosomal rDNA. Nature Structural and Molecular Biology Vol 14(2), pp. 123-130. (10.1038/nsmb1199)
- Kent, N. A., Chambers, A. L. and Downs, J. A. 2007. Dual chromatin remodeling roles for RSC during DNA double strand break induction and repair at the yeast MAT locus. Journal of Biological Chemistry 282(38), pp. 27693-27701. (10.1074/jbc.M704707200)
- Sherriff, J. A., Kent, N. A. and Mellor, J. 2007. The Isw2 chromatin-remodeling ATPase cooperates with the Fkh2 transcription factor To repress transcription of the B-type cyclin Gene CLB2. Molecular and Cellular Biology 27(8), pp. 2848-2860. (10.1128/MCB.01798-06)
- Kent, N. A., Eibert, S. M. and Mellor, J. 2004. Cbf1p Is Required for Chromatin Remodeling at Promotor - proximal CACGTG motifs in yeast. The Journal of Biological Chemistry 279(26), pp. 27116 - 27123. (10.1074/jbc.M403818200)
- Ferreiro, A., Powell, N. G., Karabetsou, N., Kent, N. A., Mellor, J. and Waters, R. 2004. Cbf1p modulates chromatin structure, transcription and repair at the Saccharomyces cerevisiae MET16 locus. Nucleic Acids Research 32(5), pp. 1617-1626. (10.1093/nar/gkh324)
- Martinez-Campa, C., Politis, P., Moreau, J., Kent, N. A., Goodall, J., Mellor, J. and Goding, C. R. 2004. Precise nucleosome positioning and the TATA box dictate requirements for the histone H4 tail and the bromodomain factor Bdf1. Molecular Cell 15(1), pp. 69-81. (10.1016/j.molcel.2004.05.022)
- Alen, C., Kent, N. A., Jones, H. S., O'Sullivan, J., Aranda, A. and Proudfoot, N. J. 2002. A role for chromatin remodeling in transcriptional termination by RNA polymerase II. Molecular Cell 10(6), pp. 1441-1452. (10.1016/S1097-2765(02)00778-5)
- Kent, N. A., Karabetsou, N., Politis, P. K. and Mellor, J. 2001. In vivo chromatin remodeling by yeast ISWI homologs Isw1p and Isw2p. Genes and Development 15, pp. 619-626. (10.1101/gad.190301)
Bywgraffiad
Derbyniais BSc. (Anrh) o Brifysgol East Anglia ym 1989, D.Phil o Brifysgol Rhydychen yn 1994, a daeth yn Gymrawd yr Academi Addysg Uwch yn 2008.
Yn ystod fy ngwaith doethurol yn labordy Jane Mellor yn Rhydychen, cefais ddiddordeb mewn sut mae pecynnu ffisegol DNA yn dylanwadu ar fynegiant genynnau, a datblygu technoleg ar gyfer treulio cromatotin burum gyda niwcleasau er mwyn mapio strwythur cromatotin sy'n dal i gael ei ddefnyddio'n eang heddiw (Kent et al., 1993; Kent and Mellor, 1995). O 1994, ymgymerais â gwaith ôl-ddoethurol yn Rhydychen yn yr Adran Biocemeg (labordy Mellor) ac Ysgol Patholeg Syr William Dunn (labordy Proudfoot), a deuthum yn Ddarlithydd Adrannol yn yr Uned Geneteg yn 2002. Yn ystod y cyfnod hwn yn Rhydychen, gweithiais ar gyfadeiladau cyd-activator/repressor sy'n defnyddio ynni o ATP hydrolysis i ailfodelu strwythur cromatin, a chwaraeodd ran allweddol wrth ddarganfod swyddogaethau cellog dosbarthiadau ISWI a CHD yr ensymau hyn (Kent et al., 2001; Alen et al., 2002, Morillion et al., 2003, Martinez-Campa et al., 2004).
Yn 2006, cymerais Ddarlithyddiaeth ym Mhrifysgol Caerdydd ac ers hynny rwyf wedi cyhoeddi papurau: sy'n nodweddu amgylcheddau cromatin yn y locus rDNA a'r telomeres (Jones et al., 2007; Loney et al., 2009; torri rôl y dosbarth SWI / SNF ATPase RSC mewn ailfodelu cromatotin-yn ystod ffurfio a thrwsio egwyl cromosomau (Kent et al., 2007; Chambers et al., 2012a); nodi rôl ar gyfer ATPase dosbarth INO80 mewn swyddogaeth centromere a sefydlogrwydd genom (Chambers et al., 2012b). Prif ffocws fy labordy bellach yw datblygu technegau cromatotin-SEQ i ddadansoddi strwythur cromatotin a swyddogaeth ar lefel genom gyfan (Kent et al., 2011; Durand-Dubief et al., 2012; Platt et al., 2013; Platt et al, 2017). Rwyf wedi parhau i archwilio ailfodelu cromatotin mewn systemau organeb enghreifftiol fel burum ymholltiad ac Arabidopsis (Gal et al., 2015; Pass et al., 2017) a hefyd celloedd dynol (Harwood et al., 2019). Chwaraeais ran hefyd wrth ddarganfod y pensaernïaeth "supernucleosome" gleiniau newydd y gwyddys eu bod bellach yn bresennol mewn celloedd archaeolegol (Maruyama et al., 2013; Ofer, S. et al., 2023).
Aelodaethau proffesiynol
Fellow of the Higher Education Academy
Contact Details
+44 29208 79036
Adeilad Syr Martin Evans, Ystafell School of Biosciences, The Sir Martin Evans Building, Museum Avenue, Cardiff, CF10 3AX, Rhodfa'r Amgueddfa, Caerdydd, CF10 3AX