![Louis Luk](https://profiles-cardiff.imgix.net/attachments/7432?w=200&h=200&auto=format&crop=faces&fit=crop&q=90")
Dr Louis Luk
(e/fe)
- Ar gael fel goruchwyliwr ôl-raddedig
Timau a rolau for Louis Luk
Uwch Ddarlithydd mewn Cemeg Fiolegol
Trosolwyg
Mae ein grŵp yn gyrru arloesedd mewn gwyddoniaeth a thechnoleg protein, pontio cemeg a bioleg i fynd i'r afael â heriau mewn cemeg feddyginiaethol, ymchwil peptid gwrthfacterol, dylunio ymgeiswyr bioactif, yn ogystal â gweithgynhyrchu biotherapiwtig. Trwy hyrwyddo rhyngwyneb y pynciau ymchwil hyn, rydym yn rhagori mewn technegau amrywiol fel synthesis peptid / protein, clonio moleciwlaidd, profion biolegol, bioleg strwythurol, a biocatalysis. Yn ogystal ag arbenigedd technegol, mae aelodau'r grŵp yn ennill profiad gwerthfawr mewn cyfathrebu, rhwydweithio a rheoli, gan hyrwyddo datblygiad cyflawn o'u proffil proffesiynol. Mae ein hyfforddiant yn arwain yn gyson at leoliadau llwyddiannus mewn diwydiannau sy'n seiliedig ar wybodaeth, gan rymuso aelodau i ffynnu yn eu gyrfaoedd gwyddonol.
Cysylltau
Gwefan bersonol: http://louisluklab.org/
Cyhoeddiad
2026
- Angelastro, A. et al. 2026. Site-specific labelling of native peptides and proteins: chemical and enzymatic strategies. Beilstein Journal of Organic Chemistry 22 , pp.857-881. (10.3762/bjoc.22.67)
2025
- Farrington, G. et al., 2025. The roles of EDA2R in ageing and disease. Aging Cell 24 (12) e70282. (10.1111/acel.70282)
2024
- Tang, T. M. S. and Luk, L. Y. P. 2024. Towards controlling activity of a peptide asparaginyl ligase (PAL) by lumazine synthetase compartmentalization. Faraday Discussions 252 , pp.403-421. (10.1039/D4FD00002A)
- Abramiuk, M. et al., 2024. Biocatalytic pathways, cascades, cells and systems: general discussion. Faraday Discussions 252 , pp.241-261. (10.1039/D4FD90023E)
- Williams, T. L. et al. 2024. Secondary amine catalysis in enzyme design: broadening protein template diversity through genetic code expansion. Angewandte Chemie International Edition (10.1002/anie.202403098)
- Lander, A. J. et al. 2024. Deciphering the synthetic and refolding strategy of a cysteine-rich domain in the tumor necrosis factor receptor (TNF-R) for racemic crystallography analysis and d-peptide ligand discovery. ACS Bio & Med Chem Au 4 (1), pp.68-76. (10.1021/acsbiomedchemau.3c00060)
2023
- Ma, M. et al. 2023. Preparing recombinant “Split AEP” for protein labeling. In: Lloyd, M. ed. Methods in Enzymology. Vol. 690, Methods in Enzymology Elsevier. , pp.501-540. (10.1016/bs.mie.2023.07.004)
- Lander, A. J. et al. 2023. Roles of inter- and intramolecular tryptophan interactions in membrane-active proteins revealed by racemic protein crystallography. Communications Chemistry 6 (1) 154. (10.1038/s42004-023-00953-y)
- Cardella, D. , Tsai, Y. and Luk, L. Y. P. 2023. Towards the use of an amino acid cleavable linker for solid-phase chemical synthesis of peptides and proteins. Organic and Biomolecular Chemistry 21 , pp.966-969. (10.1039/d2ob02198f)
- Hayes, H. C. and Luk, L. Y. P. 2023. Investigating the effects of cyclic topology on the performance of a plastic degrading enzyme for polyethylene terephthalate degradation. Scientific Reports 13 1267. (10.1038/s41598-023-27780-4)
2022
- Lander, A. J. , Jin, Y. and Luk, L. Y. P. 2022. D‐peptide and d‐protein technology: recent advances, challenges, and opportunities. ChemBioChem e202200537. (10.1002/cbic.202200537)
- Allemann, R. K. et al. 2022. Spatio-temporal control of cell death by selective delivery of photo-activatable proteins. ChemBioChem 23 (12) e202200115. (10.1002/cbic.202200115)
- Cardella, D. et al. 2022. Effect of trimethine cyanine dye- and folate-conjugation on the in vitro biological activity of proapoptotic peptides. Biomolecules 12 (5) 725. (10.3390/biom12050725)
- Galmés, M. À. et al., 2022. Computational design of an amidase by combining the best electrostatic features of two promiscuous hydrolases. Chemical Science 13 (17), pp.4779-4787. (10.1039/D2SC00778A)
2021
- Nodling, A. R. et al. 2021. The role of protein environment in catalysis by biotinylated secondary amines. Organic and Biomolecular Chemistry 19 , pp.10424-10431. (10.1039/D1OB01947C)
- Galmés, M. À. et al., 2021. Combined theoretical and experimental study to unravel the differences in promiscuous amidase activity of two nonhomologous enzymes. ACS Catalysis 11 (14), pp.8635-8644. (10.1021/acscatal.1c02150)
- Adesina, A. S. , Luk, L. Y. P. and Allemann, R. K. 2021. Cryo‐kinetics reveal dynamic effects on the chemistry of human dihydrofolate reductase. ChemBioChem 22 (14), pp.2410-2414. (10.1002/cbic.202100017)
- Tang, T. M. S. L. and Luk, L. Y. P. 2021. Asparaginyl endopeptidases: enzymology, applications and limitations. Organic and Biomolecular Chemistry 19 (23), pp.5048-5062. (10.1039/D1OB00608H)
- Hayes, H. C. , Luk, L. Y. P. and Tsai, Y. 2021. Approaches for peptide and protein cyclisation. Organic and Biomolecular Chemistry 19 (18), pp.3983-4001. (10.1039/D1OB00411E)
- Santi, N. et al. 2021. Transfer hydrogenations catalyzed by streptavidin-hosted secondary amine organocatalyst. Chemical Communications 57 (15), pp.1919-1922. (10.1039/D0CC08142F)
- Williams, T. L. et al. 2021. Transferability of N-terminal mutations of pyrrolysyl-tRNA synthetase in one species to that in another species on unnatural amino acid incorporation efficiency. Amino Acids 53 , pp.89-96. (10.1007/s00726-020-02927-z)
2020
- Thomas, S. R. et al. 2020. Exploring the chemoselectivity towards cysteine arylation by cyclometalated Au(III) compounds: new mechanistic insights. ChemBioChem 21 (21), pp.3071-3076. (10.1002/cbic.202000262)
- Adesina, A. S. et al. 2020. Electric field measurements reveal the pivotal role of Cofactor-Substrate interaction in dihydrofolate reductase catalysis. ACS Catalysis 10 (14), pp.7907-7914. (10.1021/acscatal.0c01856)
- Tang, T. M. S. et al. 2020. Use of an asparaginyl endopeptidase for chemo-enzymatic peptide and protein labeling. Chemical Science 11 (23), pp.5881-5888. (10.1039/D0SC02023K)
- Santi, N. , Morrill, L. C. and Luk, L. Y. P. 2020. Streptavidin-hosted organocatalytic aldol addition. Molecules 25 (10) 2457. (10.3390/molecules25102457)
- Nödling, A. et al. 2020. Cyanine dye mediated mitochondrial targeting enhances the anti-cancer activity of small-molecule cargoes. Chemical Communications 56 (34), pp.4672-4675. (10.1039/C9CC07931A)
- Nödling, A. R. et al. 2020. Enabling protein-hosted organocatalytic transformations. RSC Advances 10 (27), pp.16147-16161. (10.1039/D0RA01526A)
- Zheng, X. et al., 2020. Condensation of 2-((Alkylthio)(aryl)methylene)malononitrile with 1,2-Aminothiol as a novel bioorthogonal reaction for site-specific protein modification and peptide cyclization. Journal of the American Chemical Society 142 (11), pp.5097-5103. (10.1021/jacs.9b11875)
- Mills, E. M. et al. 2020. Applying switchable Cas9 variants to in vivo gene editing for therapeutic applications. Cell Biology and Toxicology 36 , pp.17-29. (10.1007/s10565-019-09488-2)
- Meier-Menches, S. M. et al. 2020. Comparative biological evaluation and G-quadruplex interaction studies of two new families of organometallic gold(I) complexes featuring N-heterocyclic carbene and alkynyl ligands. Journal of Inorganic Biochemistry 202 110844. (10.1016/j.jinorgbio.2019.110844)
2019
- Allemann, R. K. et al. 2019. Heavy enzymes and the rational redesign of protein catalysts. ChemBioChem 20 (22), pp.2807-2812. (10.1002/cbic.201900134)
- Angelastro, A. et al. 2019. Loss of hyperconjugative effects drives hydride transfer during dihydrofolate reductase catalysis. ACS Catalysis 9 (11), pp.10343-10349. (10.1021/acscatal.9b02839)
- Nodling, A. R. et al. 2019. Using genetically incorporated unnatural amino acids to control protein functions in mammalian cells. Essays in Biochemistry 63 (2), pp.237-266. (10.1042/EBC20180042)
- Scott, A. F. et al. 2019. Crystal structure and biophysical analysis of furfural detoxifying aldehyde reductase from clostridium beijerinkii. Applied and Environmental Microbiology (10.1128/AEM.00978-19)
- Patel, S. G. et al. 2019. Cell-penetrating peptide sequence and modification dependent uptake and subcellular distribution of green florescent protein in different cell lines. Scientific Reports 9 (1), pp.-. 6298. (10.1038/s41598-019-42456-8)
2018
- Nodling, A. R. et al. 2018. Reactivity and selectivity of iminium organocatalysis improved by a protein host. Angewandte Chemie International Edition 57 (38), pp.12478-12482. (10.1002/anie.201806850)
- Williams, T. L. et al. 2018. Carbapenems as water soluble organocatalysts. Wellcome Open Research 2018 (3) 107. (10.12688/wellcomeopenres.14721.1)
- Suzuki, T. et al., 2018. Switchable genome editing via genetic code expansion. Scientific Reports 8 (1) 10051. (10.1038/s41598-018-28178-3)
- Behiry, E. M. et al. 2018. Isotope substitution of promiscuous alcohol dehydrogenase reveals the origin of substrate preference in the transition state. Angewandte Chemie International Edition 57 (12), pp.3128-3131. (10.1002/anie.201712826)
- Świderek, K. et al., 2018. Reaction mechanism of organocatalytic Michael addition of nitromethane to cinnamaldehyde: a case study on catalyst regeneration and solvent effects. Journal of Physical Chemistry A 122 (1), pp.451-459. (10.1021/acs.jpca.7b11803)
2017
- Liao, J. et al., 2017. Acetylome of acinetobacter baumannii SK17 reveals a highly-conserved modification of histone-like protein HU. Frontiers in Molecular Biosciences 4 77. (10.3389/fmolb.2017.00077)
- Angelastro, A. et al. 2017. Chemoenzymatic assembly of isotopically labeled folates. Journal of the American Chemical Society 139 (37), pp.13047-13054. (10.1021/jacs.7b06358)
- Wilkins, L. C. et al. 2017. Reactions of biologically inspired hydride sources with B(C6F5)3. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 375 (2101) 20170009. (10.1098/rsta.2017.0009)
- Scott, A. F. , Luk, L. Y. P. and Allemann, R. K. 2017. Chemical ligation and isotope labeling to locate dynamic effects. In: Imperiali, B. ed. Methods in Enzymology. Vol. 596, Elsevier. , pp.23-41. (10.1016/bs.mie.2017.06.040)
- Lai, S. et al., 2017. Site-specific His/Asp phosphoproteomic analysis of prokaryotes reveals putative targets for drug resistance. BMC Microbiology 17 (1) 123. (10.1186/s12866-017-1034-2)
- Loveridge, E. J. et al. 2017. Reduction of folate by dihydrofolate reductase from thermotoga maritima. Biochemistry 56 (13), pp.1879-1886. (10.1021/acs.biochem.6b01268)
- Angelastro, A. et al. 2017. A versatile disulfide-driven recycling system for NADP+ with high cofactor turnover number. ACS Catalysis 7 (2), pp.1025-1029. (10.1021/acscatal.6b03061)
2016
- Castillo, J. P. et al., 2016. β1-subunit–induced structural rearrangements of the Ca2+- and voltage-activated K+ (BK) channel. Proceedings of the National Academy of Sciences 113 (23), pp.E3231-E3239. (10.1073/pnas.1606381113)
- Ruiz-Pernía, J. J. et al., 2016. Minimization of dynamic effects in the evolution of dihydrofolate reductase. Chemical Science 7 (5), pp.3248-3255. (10.1039/C5SC04209G)
2015
- Luk, L. Y. P. et al. 2015. Chemical ligation and isotope labeling to locate dynamic effects during catalysis by dihydrofolate reductase. Angewandte Chemie International Edition 54 (31), pp.9016-9020. (10.1002/anie.201503968)
- Luk, L. Y. P. , Loveridge, E. J. and Allemann, R. K. 2015. Protein motions and dynamic effects in enzyme catalysis. Physical Chemistry Chemical Physics 17 , pp.30817-30827. (10.1039/C5CP00794A)
- Allemann, R. K. , Loveridge, E. and Luk, L. Y. P. 2015. Protein motions, dynamic effects and thermal stability in dihydrofolate reductase from the hyperthermophile thermotoga maritima. In: Olivares-Quiroz, L. , Guzmán-López, O. and Jardón-Valadez, H. E. eds. Physical Biology of Proteins and Peptides: Theory, Experiment, and Simulation. Springer International Publishing. , pp.99-113. (10.1007/978-3-319-21687-4_6)
2014
- Luk, L. Y. P. et al. 2014. Protein isotope effects in dihydrofolate reductase from Geobacillus stearothermophilus show entropic-enthalpic com-pensatory effects on the rate constant. Journal of the American Chemical Society 136 (49), pp.17317-17323. (10.1021/ja5102536)
- Behiry, E. M. et al. 2014. Role of the occluded conformation in bacterial dihydrofolate reductases. Biochemistry 53 (29), pp.4761-4768. (10.1021/bi500507v)
- Luk, L. Y. P. , Loveridge, E. J. and Allemann, R. K. 2014. Different dynamical effects in mesophilic and hyperthermophilic dihydrofolate reductases. Journal of the American Chemical Society 136 (19), pp.6862-6865. (10.1021/ja502673h)
- Guo, J. N. et al. 2014. Thermal adaptation of dihydrofolate reductase from the moderate thermophile geobacillus stearothermophilus. Biochemistry 53 (17), pp.2855-2863. (10.1021/bi500238q)
2013
- Ruiz-Pernia, J. J. et al., 2013. Increased dynamic effects in a catalytically compromised variant of Escherichia coli dihydrofolate reductase. Journal of the American Chemical Society 135 (49), pp.18689-18696. (10.1021/ja410519h)
- Luk, L. Y. P. et al. 2013. Unraveling the role of protein dynamics in dihydrofolate reductase catalysis. Proceedings of the National Academy of Sciences of the United States of America 110 (41), pp.16344-16349. (10.1073/pnas.1312437110)
- Guo, J. et al., 2013. Effect of dimerization on dihydrofolate reductase catalysis. Biochemistry 52 (22), pp.3881-3887. (10.1021/bi4005073)
2011
- Luk, L. Y. P. , Qian, Q. and Tanner, M. E. 2011. A cope rearrangement in the reaction catalyzed by dimethylallyltryptophan synthase?. Journal of the American Chemical Society 133 (32), pp.12342-12345. (10.1021/ja2034969)
2009
- Luk, L. Y. P. and Tanner, M. E. 2009. Mechanism of dimethylallyltryptophan synthase: Evidence for a dimethylallyl cation intermediate in an aromatic prenyltransferase reaction. Journal of the American Chemical Society 131 (39), pp.13932-13933. (10.1021/ja906485u)
2007
- Luk, L. Y. P. et al. 2007. Mechanistic studies on norcoclaurine synthase of benzylisoquinoline alkaloid biosynthesis: An enzymatic Pictet-Spengler reaction. Biochemistry 46 (35), pp.10153-10161. (10.1021/bi700752n)
Adrannau llyfrau
- Ma, M. et al. 2023. Preparing recombinant “Split AEP” for protein labeling. In: Lloyd, M. ed. Methods in Enzymology. Vol. 690, Methods in Enzymology Elsevier. , pp.501-540. (10.1016/bs.mie.2023.07.004)
- Scott, A. F. , Luk, L. Y. P. and Allemann, R. K. 2017. Chemical ligation and isotope labeling to locate dynamic effects. In: Imperiali, B. ed. Methods in Enzymology. Vol. 596, Elsevier. , pp.23-41. (10.1016/bs.mie.2017.06.040)
- Allemann, R. K. , Loveridge, E. and Luk, L. Y. P. 2015. Protein motions, dynamic effects and thermal stability in dihydrofolate reductase from the hyperthermophile thermotoga maritima. In: Olivares-Quiroz, L. , Guzmán-López, O. and Jardón-Valadez, H. E. eds. Physical Biology of Proteins and Peptides: Theory, Experiment, and Simulation. Springer International Publishing. , pp.99-113. (10.1007/978-3-319-21687-4_6)
Erthyglau
- Angelastro, A. et al. 2026. Site-specific labelling of native peptides and proteins: chemical and enzymatic strategies. Beilstein Journal of Organic Chemistry 22 , pp.857-881. (10.3762/bjoc.22.67)
- Farrington, G. et al., 2025. The roles of EDA2R in ageing and disease. Aging Cell 24 (12) e70282. (10.1111/acel.70282)
- Tang, T. M. S. and Luk, L. Y. P. 2024. Towards controlling activity of a peptide asparaginyl ligase (PAL) by lumazine synthetase compartmentalization. Faraday Discussions 252 , pp.403-421. (10.1039/D4FD00002A)
- Abramiuk, M. et al., 2024. Biocatalytic pathways, cascades, cells and systems: general discussion. Faraday Discussions 252 , pp.241-261. (10.1039/D4FD90023E)
- Williams, T. L. et al. 2024. Secondary amine catalysis in enzyme design: broadening protein template diversity through genetic code expansion. Angewandte Chemie International Edition (10.1002/anie.202403098)
- Lander, A. J. et al. 2024. Deciphering the synthetic and refolding strategy of a cysteine-rich domain in the tumor necrosis factor receptor (TNF-R) for racemic crystallography analysis and d-peptide ligand discovery. ACS Bio & Med Chem Au 4 (1), pp.68-76. (10.1021/acsbiomedchemau.3c00060)
- Lander, A. J. et al. 2023. Roles of inter- and intramolecular tryptophan interactions in membrane-active proteins revealed by racemic protein crystallography. Communications Chemistry 6 (1) 154. (10.1038/s42004-023-00953-y)
- Cardella, D. , Tsai, Y. and Luk, L. Y. P. 2023. Towards the use of an amino acid cleavable linker for solid-phase chemical synthesis of peptides and proteins. Organic and Biomolecular Chemistry 21 , pp.966-969. (10.1039/d2ob02198f)
- Hayes, H. C. and Luk, L. Y. P. 2023. Investigating the effects of cyclic topology on the performance of a plastic degrading enzyme for polyethylene terephthalate degradation. Scientific Reports 13 1267. (10.1038/s41598-023-27780-4)
- Lander, A. J. , Jin, Y. and Luk, L. Y. P. 2022. D‐peptide and d‐protein technology: recent advances, challenges, and opportunities. ChemBioChem e202200537. (10.1002/cbic.202200537)
- Allemann, R. K. et al. 2022. Spatio-temporal control of cell death by selective delivery of photo-activatable proteins. ChemBioChem 23 (12) e202200115. (10.1002/cbic.202200115)
- Cardella, D. et al. 2022. Effect of trimethine cyanine dye- and folate-conjugation on the in vitro biological activity of proapoptotic peptides. Biomolecules 12 (5) 725. (10.3390/biom12050725)
- Galmés, M. À. et al., 2022. Computational design of an amidase by combining the best electrostatic features of two promiscuous hydrolases. Chemical Science 13 (17), pp.4779-4787. (10.1039/D2SC00778A)
- Nodling, A. R. et al. 2021. The role of protein environment in catalysis by biotinylated secondary amines. Organic and Biomolecular Chemistry 19 , pp.10424-10431. (10.1039/D1OB01947C)
- Galmés, M. À. et al., 2021. Combined theoretical and experimental study to unravel the differences in promiscuous amidase activity of two nonhomologous enzymes. ACS Catalysis 11 (14), pp.8635-8644. (10.1021/acscatal.1c02150)
- Adesina, A. S. , Luk, L. Y. P. and Allemann, R. K. 2021. Cryo‐kinetics reveal dynamic effects on the chemistry of human dihydrofolate reductase. ChemBioChem 22 (14), pp.2410-2414. (10.1002/cbic.202100017)
- Tang, T. M. S. L. and Luk, L. Y. P. 2021. Asparaginyl endopeptidases: enzymology, applications and limitations. Organic and Biomolecular Chemistry 19 (23), pp.5048-5062. (10.1039/D1OB00608H)
- Hayes, H. C. , Luk, L. Y. P. and Tsai, Y. 2021. Approaches for peptide and protein cyclisation. Organic and Biomolecular Chemistry 19 (18), pp.3983-4001. (10.1039/D1OB00411E)
- Santi, N. et al. 2021. Transfer hydrogenations catalyzed by streptavidin-hosted secondary amine organocatalyst. Chemical Communications 57 (15), pp.1919-1922. (10.1039/D0CC08142F)
- Williams, T. L. et al. 2021. Transferability of N-terminal mutations of pyrrolysyl-tRNA synthetase in one species to that in another species on unnatural amino acid incorporation efficiency. Amino Acids 53 , pp.89-96. (10.1007/s00726-020-02927-z)
- Thomas, S. R. et al. 2020. Exploring the chemoselectivity towards cysteine arylation by cyclometalated Au(III) compounds: new mechanistic insights. ChemBioChem 21 (21), pp.3071-3076. (10.1002/cbic.202000262)
- Adesina, A. S. et al. 2020. Electric field measurements reveal the pivotal role of Cofactor-Substrate interaction in dihydrofolate reductase catalysis. ACS Catalysis 10 (14), pp.7907-7914. (10.1021/acscatal.0c01856)
- Tang, T. M. S. et al. 2020. Use of an asparaginyl endopeptidase for chemo-enzymatic peptide and protein labeling. Chemical Science 11 (23), pp.5881-5888. (10.1039/D0SC02023K)
- Santi, N. , Morrill, L. C. and Luk, L. Y. P. 2020. Streptavidin-hosted organocatalytic aldol addition. Molecules 25 (10) 2457. (10.3390/molecules25102457)
- Nödling, A. et al. 2020. Cyanine dye mediated mitochondrial targeting enhances the anti-cancer activity of small-molecule cargoes. Chemical Communications 56 (34), pp.4672-4675. (10.1039/C9CC07931A)
- Nödling, A. R. et al. 2020. Enabling protein-hosted organocatalytic transformations. RSC Advances 10 (27), pp.16147-16161. (10.1039/D0RA01526A)
- Zheng, X. et al., 2020. Condensation of 2-((Alkylthio)(aryl)methylene)malononitrile with 1,2-Aminothiol as a novel bioorthogonal reaction for site-specific protein modification and peptide cyclization. Journal of the American Chemical Society 142 (11), pp.5097-5103. (10.1021/jacs.9b11875)
- Mills, E. M. et al. 2020. Applying switchable Cas9 variants to in vivo gene editing for therapeutic applications. Cell Biology and Toxicology 36 , pp.17-29. (10.1007/s10565-019-09488-2)
- Meier-Menches, S. M. et al. 2020. Comparative biological evaluation and G-quadruplex interaction studies of two new families of organometallic gold(I) complexes featuring N-heterocyclic carbene and alkynyl ligands. Journal of Inorganic Biochemistry 202 110844. (10.1016/j.jinorgbio.2019.110844)
- Allemann, R. K. et al. 2019. Heavy enzymes and the rational redesign of protein catalysts. ChemBioChem 20 (22), pp.2807-2812. (10.1002/cbic.201900134)
- Angelastro, A. et al. 2019. Loss of hyperconjugative effects drives hydride transfer during dihydrofolate reductase catalysis. ACS Catalysis 9 (11), pp.10343-10349. (10.1021/acscatal.9b02839)
- Nodling, A. R. et al. 2019. Using genetically incorporated unnatural amino acids to control protein functions in mammalian cells. Essays in Biochemistry 63 (2), pp.237-266. (10.1042/EBC20180042)
- Scott, A. F. et al. 2019. Crystal structure and biophysical analysis of furfural detoxifying aldehyde reductase from clostridium beijerinkii. Applied and Environmental Microbiology (10.1128/AEM.00978-19)
- Patel, S. G. et al. 2019. Cell-penetrating peptide sequence and modification dependent uptake and subcellular distribution of green florescent protein in different cell lines. Scientific Reports 9 (1), pp.-. 6298. (10.1038/s41598-019-42456-8)
- Nodling, A. R. et al. 2018. Reactivity and selectivity of iminium organocatalysis improved by a protein host. Angewandte Chemie International Edition 57 (38), pp.12478-12482. (10.1002/anie.201806850)
- Williams, T. L. et al. 2018. Carbapenems as water soluble organocatalysts. Wellcome Open Research 2018 (3) 107. (10.12688/wellcomeopenres.14721.1)
- Suzuki, T. et al., 2018. Switchable genome editing via genetic code expansion. Scientific Reports 8 (1) 10051. (10.1038/s41598-018-28178-3)
- Behiry, E. M. et al. 2018. Isotope substitution of promiscuous alcohol dehydrogenase reveals the origin of substrate preference in the transition state. Angewandte Chemie International Edition 57 (12), pp.3128-3131. (10.1002/anie.201712826)
- Świderek, K. et al., 2018. Reaction mechanism of organocatalytic Michael addition of nitromethane to cinnamaldehyde: a case study on catalyst regeneration and solvent effects. Journal of Physical Chemistry A 122 (1), pp.451-459. (10.1021/acs.jpca.7b11803)
- Liao, J. et al., 2017. Acetylome of acinetobacter baumannii SK17 reveals a highly-conserved modification of histone-like protein HU. Frontiers in Molecular Biosciences 4 77. (10.3389/fmolb.2017.00077)
- Angelastro, A. et al. 2017. Chemoenzymatic assembly of isotopically labeled folates. Journal of the American Chemical Society 139 (37), pp.13047-13054. (10.1021/jacs.7b06358)
- Wilkins, L. C. et al. 2017. Reactions of biologically inspired hydride sources with B(C6F5)3. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 375 (2101) 20170009. (10.1098/rsta.2017.0009)
- Lai, S. et al., 2017. Site-specific His/Asp phosphoproteomic analysis of prokaryotes reveals putative targets for drug resistance. BMC Microbiology 17 (1) 123. (10.1186/s12866-017-1034-2)
- Loveridge, E. J. et al. 2017. Reduction of folate by dihydrofolate reductase from thermotoga maritima. Biochemistry 56 (13), pp.1879-1886. (10.1021/acs.biochem.6b01268)
- Angelastro, A. et al. 2017. A versatile disulfide-driven recycling system for NADP+ with high cofactor turnover number. ACS Catalysis 7 (2), pp.1025-1029. (10.1021/acscatal.6b03061)
- Castillo, J. P. et al., 2016. β1-subunit–induced structural rearrangements of the Ca2+- and voltage-activated K+ (BK) channel. Proceedings of the National Academy of Sciences 113 (23), pp.E3231-E3239. (10.1073/pnas.1606381113)
- Ruiz-Pernía, J. J. et al., 2016. Minimization of dynamic effects in the evolution of dihydrofolate reductase. Chemical Science 7 (5), pp.3248-3255. (10.1039/C5SC04209G)
- Luk, L. Y. P. et al. 2015. Chemical ligation and isotope labeling to locate dynamic effects during catalysis by dihydrofolate reductase. Angewandte Chemie International Edition 54 (31), pp.9016-9020. (10.1002/anie.201503968)
- Luk, L. Y. P. , Loveridge, E. J. and Allemann, R. K. 2015. Protein motions and dynamic effects in enzyme catalysis. Physical Chemistry Chemical Physics 17 , pp.30817-30827. (10.1039/C5CP00794A)
- Luk, L. Y. P. et al. 2014. Protein isotope effects in dihydrofolate reductase from Geobacillus stearothermophilus show entropic-enthalpic com-pensatory effects on the rate constant. Journal of the American Chemical Society 136 (49), pp.17317-17323. (10.1021/ja5102536)
- Behiry, E. M. et al. 2014. Role of the occluded conformation in bacterial dihydrofolate reductases. Biochemistry 53 (29), pp.4761-4768. (10.1021/bi500507v)
- Luk, L. Y. P. , Loveridge, E. J. and Allemann, R. K. 2014. Different dynamical effects in mesophilic and hyperthermophilic dihydrofolate reductases. Journal of the American Chemical Society 136 (19), pp.6862-6865. (10.1021/ja502673h)
- Guo, J. N. et al. 2014. Thermal adaptation of dihydrofolate reductase from the moderate thermophile geobacillus stearothermophilus. Biochemistry 53 (17), pp.2855-2863. (10.1021/bi500238q)
- Ruiz-Pernia, J. J. et al., 2013. Increased dynamic effects in a catalytically compromised variant of Escherichia coli dihydrofolate reductase. Journal of the American Chemical Society 135 (49), pp.18689-18696. (10.1021/ja410519h)
- Luk, L. Y. P. et al. 2013. Unraveling the role of protein dynamics in dihydrofolate reductase catalysis. Proceedings of the National Academy of Sciences of the United States of America 110 (41), pp.16344-16349. (10.1073/pnas.1312437110)
- Guo, J. et al., 2013. Effect of dimerization on dihydrofolate reductase catalysis. Biochemistry 52 (22), pp.3881-3887. (10.1021/bi4005073)
- Luk, L. Y. P. , Qian, Q. and Tanner, M. E. 2011. A cope rearrangement in the reaction catalyzed by dimethylallyltryptophan synthase?. Journal of the American Chemical Society 133 (32), pp.12342-12345. (10.1021/ja2034969)
- Luk, L. Y. P. and Tanner, M. E. 2009. Mechanism of dimethylallyltryptophan synthase: Evidence for a dimethylallyl cation intermediate in an aromatic prenyltransferase reaction. Journal of the American Chemical Society 131 (39), pp.13932-13933. (10.1021/ja906485u)
- Luk, L. Y. P. et al. 2007. Mechanistic studies on norcoclaurine synthase of benzylisoquinoline alkaloid biosynthesis: An enzymatic Pictet-Spengler reaction. Biochemistry 46 (35), pp.10153-10161. (10.1021/bi700752n)
Ymchwil
Mae ein hymchwil yn grymuso gwyddoniaeth protein a'i chymwysiadau, wedi'u categoreiddio'n fras yn y meysydd canlynol:
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Labelu protein graddadwy a manwl gywir trwy Biocatalysis: Hyrwyddo Dylunio a Gweithgynhyrchu Biotherapiwtig.
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Darganfod rhwymwyr peptid fel ymgeiswyr arweiniol: integreiddio bioleg synthetig i ddatblygiad cemeg feddyginiaethol.
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Synthesis Ligand Bifunctional Catalysed Enzyme-Catalysed: Hyrwyddo Diraddio Protein wedi'i Dargedu ar gyfer Ceisiadau Therapiwtig
Mae cynnydd yn y prosiectau hyn yn galluogi amlygiad mewn amrywiaeth eang o dechnegau gwerthfawr, gan gynnwys synthesis organig, clonio moleciwlaidd, peirianneg peptid / protein, peirianneg ensymau, sbectrometreg màs a chrisialaeth protein. Mae'r sgiliau hyn yn cael eu cyflogi'n rheolaidd mewn labordai academaidd a diwydiannol, gan ddarparu hyfforddeion ar gyfer llwyddiant mewn gyrfaoedd gwyddonol amrywiol.
Addysgu
CHT352: Technegau Darganfod Cyffuriau
CHT235: Strwythur a Thechnegau mewn Bioleg Gemegol
CH3412: Cemeg Supramoleciwlaidd
CH3317: Biocatalysis wedi'i beiriannu
Bywgraffiad
Anrhydeddau a dyfarniadau
BBSRC. 03/2021-02/2024. Prif Ymchwilydd. EC-LOOP.
Cynllun Cyfnewid Rhyngwladol y Gymdeithas Frenhinol. 03/2018-02/2020. Prif Ymchwilydd. Protein nanoreactor: sy'n crynhoi biocatalydd gwenwynig ar gyfer cynhyrchu peptidau cylchol gwerth uchel.
Grant Ymchwil y Gymdeithas Frenhinol. 03/2018-02/2019. Prif Ymchwilydd. eplesu ar raddfa fawr o broteinau cylchol.
Ymddiriedolaeth Leverhulme. 11/2017-10/2020. Prif Ymchwilydd. Dylunio ensymau artiffisial organocatalytig amlbwrpas.
Wellcome Seed Trust. 08/2016-08/2018. Prif Ymchwilydd. Dihydrofolate reductase interactome: rhwydwaith anhysbys sy'n gallu rheoli synthesis DNA a dyblygu celloedd.
BBSRC. 08/2014-07/2017. Cyd-ymchwilydd. Dynameg gyplysedig adwaith mewn catalysis DHFR.
BBSRC. 04/2017-03/2020. Cyd-ymchwilydd. Rheolaeth di-olrhain, anfewnwthiol a gofodol o weithgarwch protein mewn celloedd.
Aelodaethau proffesiynol
Swyddog Cyfathrebu RSC PPSG ac Aelod o'r Pwyllgor
RSC CBBG Aelod Pwyllgor
Pennaeth Pwyllgor Moeseg Ysgol Cemeg Caerdydd
Aelod o Bwyllgor EDI yn Ysgol Cemeg Caerdydd
Safleoedd academaidd blaenorol
2020-presennol: Uwch Ddarlithydd
2019-2020: Darlithydd
2016-2020: Cymrawd Ymchwil Prifysgol Caerdydd
2012-2020: Ymchwilydd Ôl-ddoethurol (Prifysgol Caerdydd)
2010-2012: Cydymaith Ymchwil Ôl-ddoethurol (Prifysgol Chicago)
2004-2010: PhD Cemeg (Prifysgol British Columbia)
1999-2004: BSc Cemeg a Microbioleg ac Imiwnoleg (Prifysgol British Columbia)
Ymrwymiadau siarad cyhoeddus
- Cemeg, St Andrews, Yr Alban, y DU, 2024
- Cynhadledd Synthesis Peptid Ewropeaidd, Y Weriniaeth Tsiec, 2024
- Cymdeithas Peptid Prydain: Cemeg a Bioleg Peptidau, Lerpwl, y DU, 2024
- Cemeg, Coleg y Brenin Llundain, Llundain, y DU, 2024
- Sefydliad Biotechnoleg Manceinion (MIB), Manceinion, y DU, 2024
- Tueddiadau mewn catalysis ensym, Sbaen, 2022
- CEM New Frontiers inPeptide Synthesis, Lerpwl, UK, 2022
- Cemeg, Prifysgol Nanyang, Singapore, 2022
- Ysgol Fferylliaeth Caerdydd, y DU, 2020
- Cemeg, Prifysgol Leeds, y DU, 2020
- Cemeg, Prifysgol Gogledd-ddwyreiniol, Tsieina, 2019
- Adran Organig RSC Southwest Rhanbarthol, Rhydychen, y DU, 2019
- Cemeg, Prifysgol British Columbia, Canada, 2019
- Gwyddorau Moleciwlaidd, Prifysgol Gorllewin Awstralia, Awstralia, 2019
- Cemeg (grŵp Hilvert), ETH, Y Swistir, 2019
- Cemeg, Prifysgol Jaume I, Sbaen, 2019
- Cemeg, Prifysgol Xiamen, Tsieina, 2019
- Gwyddoniaeth Gymhwysol, Prifysgol Polytechnig Hong Kong, Tsieina, 2019
- Cemeg a Biocemeg, Prifysgol Laurier Wilfred, Canada, 2019
Contact Details
[email protected]
+44 29225 10161
Y Prif Adeilad, Llawr 1af , Ystafell 1.54, Plas y Parc, Caerdydd, CF10 3AT
+44 29225 10161
Y Prif Adeilad, Llawr 1af , Ystafell 1.54, Plas y Parc, Caerdydd, CF10 3AT
Themâu ymchwil
Arbenigeddau
- bioleg gemegol
- Cemeg meddyginiaethol a biomolecwlaidd
- Biocemeg feddygol proteinau a peptidau
- Biocatalysis a thechnoleg ensym
- Cemeg organig