Dr Louis Luk

2024

• Tang, T. M. S. and Luk, L. Y. P. 2024. Towards controlling activity of a peptide asparaginyl ligase (PAL) by lumazine synthetase compartmentalization. Faraday Discussions 252, pp. 403-421. (10.1039/D4FD00002A)
• Abramiuk, M. et al. 2024. Biocatalytic pathways, cascades, cells and systems: general discussion. Faraday Discussions 252, pp. 241-261. (10.1039/D4FD90023E)
• Williams, T. L. et al. 2024. Secondary amine catalysis in enzyme design: broadening protein template diversity through genetic code expansion. Angewandte Chemie International Edition (10.1002/anie.202403098)
• Lander, A. J., Kong, Y., Jin, Y., Wu, C. and Luk, L. Y. P. 2024. Deciphering the synthetic and refolding strategy of a cysteine-rich domain in the tumor necrosis factor receptor (TNF-R) for racemic crystallography analysis and d-peptide ligand discovery. ACS Bio & Med Chem Au 4(1), pp. 68-76. (10.1021/acsbiomedchemau.3c00060)

2023

• Ma, M., Tang, S. T., Dickerson, M. T. and Luk, L. Y. 2023. Preparing recombinant “Split AEP” for protein labeling. In: Lloyd, M. ed. Methods in Enzymology., Vol. 690. Methods in Enzymology Elsevier, pp. 501-540., (10.1016/bs.mie.2023.07.004)
• Lander, A. J., Mercado, L. D., Li, X., Taily, I. M., Findlay, B. L., Jin, Y. and Luk, L. Y. P. 2023. Roles of inter- and intramolecular tryptophan interactions in membrane-active proteins revealed by racemic protein crystallography. Communications Chemistry 6(1), article number: 154. (10.1038/s42004-023-00953-y)
• Cardella, D., Tsai, Y. and Luk, L. Y. P. 2023. Towards the use of an amino acid cleavable linker for solid-phase chemical synthesis of peptides and proteins. Organic and Biomolecular Chemistry 21, pp. 966-969. (10.1039/d2ob02198f)
• Hayes, H. C. and Luk, L. Y. P. 2023. Investigating the effects of cyclic topology on the performance of a plastic degrading enzyme for polyethylene terephthalate degradation. Scientific Reports 13, article number: 1267. (10.1038/s41598-023-27780-4)

2022

• Lander, A. J., Jin, Y. and Luk, L. Y. P. 2022. D‐peptide and d‐protein technology: recent advances, challenges, and opportunities. ChemBioChem, article number: e202200537. (10.1002/cbic.202200537)
• Allemann, R. K., Samperio, R., Mart, R., Luk, L., Tsai, Y., Jones, A. and Cruz Samperio, R. 2022. Spatio-temporal control of cell death by selective delivery of photo-activatable proteins. ChemBioChem 23(12), article number: e202200115. (10.1002/cbic.202200115)
• Cardella, D., Deng, W., Luk, L. Y. P. and Tsai, Y. 2022. Effect of trimethine cyanine dye- and folate-conjugation on the in vitro biological activity of proapoptotic peptides. Biomolecules 12(5), article number: 725. (10.3390/biom12050725)
• Galmés, M. ?., Nodling, A. R., He, K., Luk, L. Y. P., Swiderek, K. and Moliner, V. 2022. Computational design of an amidase by combining the best electrostatic features of two promiscuous hydrolases. Chemical Science 13(17), pp. 4779-4787. (10.1039/D2SC00778A)

2021

• Nodling, A. R. et al. 2021. The role of protein environment in catalysis by biotinylated secondary amines. Organic and Biomolecular Chemistry 19, pp. 10424-10431. (10.1039/D1OB01947C)
• Galmés, M. ?., Nodling, A. R., Luk, L., Swiderek, K. and Moliner, V. 2021. Combined theoretical and experimental study to unravel the differences in promiscuous amidase activity of two nonhomologous enzymes. ACS Catalysis 11(14), pp. 8635-8644. (10.1021/acscatal.1c02150)
• Adesina, A. S., Luk, L. Y. P. and Allemann, R. K. 2021. Cryo‐kinetics reveal dynamic effects on the chemistry of human dihydrofolate reductase. ChemBioChem 22(14), pp. 2410-2414. (10.1002/cbic.202100017)
• Tang, T. M. S. L. and Luk, L. Y. P. 2021. Asparaginyl endopeptidases: enzymology, applications and limitations. Organic and Biomolecular Chemistry 19(23), pp. 5048-5062. (10.1039/D1OB00608H)
• Hayes, H. C., Luk, L. Y. P. and Tsai, Y. 2021. Approaches for peptide and protein cyclisation. Organic and Biomolecular Chemistry 19(18), pp. 3983-4001. (10.1039/D1OB00411E)
• Santi, N., Morrill, L. C., Swiderek, K., Moliner, V. and Luk, L. Y. P. 2021. Transfer hydrogenations catalyzed by streptavidin-hosted secondary amine organocatalyst. Chemical Communications 57(15), pp. 1919-1922. (10.1039/D0CC08142F)
• Williams, T. L., Iskandar, D. J., Nödling, A. R., Tan, Y., Luk, L. Y. P. and Tsai, Y. 2021. Transferability of N-terminal mutations of pyrrolysyl-tRNA synthetase in one species to that in another species on unnatural amino acid incorporation efficiency. Amino Acids 53, pp. 89-96. (10.1007/s00726-020-02927-z)

2020

• Thomas, S. R. et al. 2020. Exploring the chemoselectivity towards cysteine arylation by cyclometalated Au(III) compounds: new mechanistic insights. ChemBioChem 21(21), pp. 3071-3076. (10.1002/cbic.202000262)
• Adesina, A. S., Swiderek, K., Luk, L. Y. P., Moliner, V. and Allemann, R. K. 2020. Electric field measurements reveal the pivotal role of Cofactor-Substrate interaction in dihydrofolate reductase catalysis. ACS Catalysis 10(14), pp. 7907-7914. (10.1021/acscatal.0c01856)
• Tang, T. M. S., Cardella, D., Lander, A. J., Li, X., Escudero, J. S., Tsai, Y. and Luk, L. Y. P. 2020. Use of an asparaginyl endopeptidase for chemo-enzymatic peptide and protein labeling. Chemical Science 11(23), pp. 5881-5888. (10.1039/D0SC02023K)
• Santi, N., Morrill, L. C. and Luk, L. Y. P. 2020. Streptavidin-hosted organocatalytic aldol addition. Molecules 25(10), article number: 2457. (10.3390/molecules25102457)
• Nödling, A. et al. 2020. Cyanine dye mediated mitochondrial targeting enhances the anti-cancer activity of small-molecule cargoes. Chemical Communications 56(34), pp. 4672-4675. (10.1039/C9CC07931A)
• Nödling, A. R., Santi, N., Williams, T. L., Tsai, Y. and Luk, L. Y. P. 2020. Enabling protein-hosted organocatalytic transformations. RSC Advances 10(27), pp. 16147-16161. (10.1039/D0RA01526A)
• Zheng, X. et al. 2020. Condensation of 2-((Alkylthio)(aryl)methylene)malononitrile with 1,2-Aminothiol as a novel bioorthogonal reaction for site-specific protein modification and peptide cyclization. Journal of the American Chemical Society 142(11), pp. 5097-5103. (10.1021/jacs.9b11875)
• Mills, E. M., Barlow, V. L., Luk, L. Y. P. and Tsai, Y. 2020. Applying switchable Cas9 variants to in vivo gene editing for therapeutic applications. Cell Biology and Toxicology 36, pp. 17-29. (10.1007/s10565-019-09488-2)
• Meier-Menches, S. M. et al. 2020. Comparative biological evaluation and G-quadruplex interaction studies of two new families of organometallic gold(I) complexes featuring N-heterocyclic carbene and alkynyl ligands. Journal of Inorganic Biochemistry 202, article number: 110844. (10.1016/j.jinorgbio.2019.110844)

2019

• Allemann, R. K., Scott, A. F., Luk, L. Y., Tuñón, I. and Moliner, V. 2019. Heavy enzymes and the rational redesign of protein catalysts. ChemBioChem 20(22), pp. 2807-2812. (10.1002/cbic.201900134)
• Angelastro, A., Ruiz-Pernía, J. J., Tuñón, I., Moliner, V., Luk, L. Y. P. and Allemann, R. K. 2019. Loss of hyperconjugative effects drives hydride transfer during dihydrofolate reductase catalysis. ACS Catalysis 9(11), pp. 10343-10349. (10.1021/acscatal.9b02839)
• Nodling, A. R., Spear, L. A., Williams, T. L., Luk, L. Y. and Tsai, Y. 2019. Using genetically incorporated unnatural amino acids to control protein functions in mammalian cells. Essays in Biochemistry 63(2), pp. 237-266. (10.1042/EBC20180042)
• Scott, A. F., Cresser-Brown, J., Williams, T. L., Rizkallah, P. J., Jin, Y., Luk, L. Y. and Allemann, R. K. 2019. Crystal structure and biophysical analysis of furfural detoxifying aldehyde reductase from clostridium beijerinkii. Applied and Environmental Microbiology (10.1128/AEM.00978-19)
• Patel, S. G. et al. 2019. Cell-penetrating peptide sequence and modification dependent uptake and subcellular distribution of green florescent protein in different cell lines. Scientific Reports 9(1), pp. -., article number: 6298. (10.1038/s41598-019-42456-8)

2018

• Nodling, A. R. et al. 2018. Reactivity and selectivity of iminium organocatalysis improved by a protein host. Angewandte Chemie International Edition 57(38), pp. 12478-12482. (10.1002/anie.201806850)
• Williams, T. L., Nodling, A., Tsai, Y. and Luk, L. Y. P. 2018. Carbapenems as water soluble organocatalysts. Wellcome Open Research 2018(3), article number: 107. (10.12688/wellcomeopenres.14721.1)
• Suzuki, T., Asami, M., Patel, S. G., Luk, L. Y. P., Tsai, Y. and Perry, A. C. F. 2018. Switchable genome editing via genetic code expansion. Scientific Reports 8(1), article number: 10051. (10.1038/s41598-018-28178-3)
• Behiry, E. M., Ruiz-Pernia, J. J., Luk, L., Tunon, I., Moliner, V. and Allemann, R. K. 2018. Isotope substitution of promiscuous alcohol dehydrogenase reveals the origin of substrate preference in the transition state. Angewandte Chemie International Edition 57(12), pp. 3128-3131. (10.1002/anie.201712826)
• Świderek, K., Nödling, A., Tsai, Y., Luk, L. and Moliner, V. 2018. Reaction mechanism of organocatalytic Michael addition of nitromethane to cinnamaldehyde: a case study on catalyst regeneration and solvent effects. Journal of Physical Chemistry A 122(1), pp. 451-459. (10.1021/acs.jpca.7b11803)

2017

• Liao, J. et al. 2017. Acetylome of acinetobacter baumannii SK17 reveals a highly-conserved modification of histone-like protein HU. Frontiers in Molecular Biosciences 4, article number: 77. (10.3389/fmolb.2017.00077)
• Angelastro, A., Dawson, W. M., Luk, L. Y. P., Loveridge, E. J. and Allemann, R. K. 2017. Chemoenzymatic assembly of isotopically labeled folates. Journal of the American Chemical Society 139(37), pp. 13047-13054. (10.1021/jacs.7b06358)
• Wilkins, L. C., Santi, N., Luk, L. Y. P. and Melen, R. L. 2017. Reactions of biologically inspired hydride sources with B(C6F5)3. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 375(2101), article number: 20170009. (10.1098/rsta.2017.0009)
• Scott, A. F., Luk, L. Y. P. and Allemann, R. K. 2017. Chemical ligation and isotope labeling to locate dynamic effects. In: Imperiali, B. ed. Methods in Enzymology., Vol. 596. Elsevier, pp. 23-41., (10.1016/bs.mie.2017.06.040)
• Lai, S. et al. 2017. Site-specific His/Asp phosphoproteomic analysis of prokaryotes reveals putative targets for drug resistance. BMC Microbiology 17(1), article number: 123. (10.1186/s12866-017-1034-2)
• Loveridge, E. J. et al. 2017. Reduction of folate by dihydrofolate reductase from thermotoga maritima. Biochemistry 56(13), pp. 1879-1886. (10.1021/acs.biochem.6b01268)
• Angelastro, A., Dawson, W. M., Luk, L. Y. P. and Allemann, R. K. 2017. A versatile disulfide-driven recycling system for NADP+ with high cofactor turnover number. ACS Catalysis 7(2), pp. 1025-1029. (10.1021/acscatal.6b03061)

2016

• Castillo, J. P. et al. 2016. β1-subunit–induced structural rearrangements of the Ca2+- and voltage-activated K+ (BK) channel. Proceedings of the National Academy of Sciences 113(23), pp. E3231-E3239. (10.1073/pnas.1606381113)
• Ruiz-Pernía, J. J., Behiry, E., Luk, L. Y. P., Loveridge, E. J., Tuñón, I., Moliner, V. and Allemann, R. K. 2016. Minimization of dynamic effects in the evolution of dihydrofolate reductase. Chemical Science 7(5), pp. 3248-3255. (10.1039/C5SC04209G)

2015

• Luk, L. Y. P., Ruiz-Pernía, J. J., Adesina, A. S., Loveridge, E. J., Tuñón, I., Moliner, V. and Allemann, R. K. 2015. Chemical ligation and isotope labeling to locate dynamic effects during catalysis by dihydrofolate reductase. Angewandte Chemie International Edition 54(31), pp. 9016-9020. (10.1002/anie.201503968)
• Luk, L. Y. P., Loveridge, E. J. and Allemann, R. K. 2015. Protein motions and dynamic effects in enzyme catalysis. Physical Chemistry Chemical Physics 17, pp. 30817-30827. (10.1039/C5CP00794A)
• Allemann, R. K., Loveridge, E. and Luk, L. Y. P. 2015. Protein motions, dynamic effects and thermal stability in dihydrofolate reductase from the hyperthermophile thermotoga maritima. In: Olivares-Quiroz, L., Guzmán-López, O. and Jardón-Valadez, H. E. eds. Physical Biology of Proteins and Peptides: Theory, Experiment, and Simulation. Springer International Publishing, pp. 99-113., (10.1007/978-3-319-21687-4_6)

2014

• Luk, L. Y. P., Ruiz-Pernia, J. J., Dawson, W. M., Loveridge, E. J., Tuñón, I., Moliner, V. and Allemann, R. K. 2014. Protein isotope effects in dihydrofolate reductase from Geobacillus stearothermophilus show entropic-enthalpic com-pensatory effects on the rate constant. Journal of the American Chemical Society 136(49), pp. 17317-17323. (10.1021/ja5102536)
• Behiry, E. M., Luk, L. Y. P., Matthews, S. M., Loveridge, E. J. and Allemann, R. K. 2014. Role of the occluded conformation in bacterial dihydrofolate reductases. Biochemistry 53(29), pp. 4761-4768. (10.1021/bi500507v)
• Luk, L. Y. P., Loveridge, E. J. and Allemann, R. K. 2014. Different dynamical effects in mesophilic and hyperthermophilic dihydrofolate reductases. Journal of the American Chemical Society 136(19), pp. 6862-6865. (10.1021/ja502673h)
• Guo, J. N., Luk, L. Y. P., Loveridge, E. J. and Allemann, R. K. 2014. Thermal adaptation of dihydrofolate reductase from the moderate thermophile geobacillus stearothermophilus. Biochemistry 53(17), pp. 2855-2863. (10.1021/bi500238q)

2013

• Ruiz-Pernia, J. J. et al. 2013. Increased dynamic effects in a catalytically compromised variant of Escherichia coli dihydrofolate reductase. Journal of the American Chemical Society 135(49), pp. 18689-18696. (10.1021/ja410519h)
• Luk, L. Y. P. et al. 2013. Unraveling the role of protein dynamics in dihydrofolate reductase catalysis. Proceedings of the National Academy of Sciences of the United States of America 110(41), pp. 16344-16349. (10.1073/pnas.1312437110)
• Guo, J., Loveridge, E. J., Luk, L. Y. P. and Allemann, R. K. 2013. Effect of dimerization on dihydrofolate reductase catalysis. Biochemistry 52(22), pp. 3881-3887. (10.1021/bi4005073)

2011

• Luk, L. Y. P., Qian, Q. and Tanner, M. E. 2011. A cope rearrangement in the reaction catalyzed by dimethylallyltryptophan synthase?. Journal of the American Chemical Society 133(32), pp. 12342-12345. (10.1021/ja2034969)

2009

• Luk, L. Y. P. and Tanner, M. E. 2009. Mechanism of dimethylallyltryptophan synthase: Evidence for a dimethylallyl cation intermediate in an aromatic prenyltransferase reaction. Journal of the American Chemical Society 131(39), pp. 13932-13933. (10.1021/ja906485u)

2007

• Luk, L. Y. P., Bunn, S., Liscombe, D. K., Facchini, P. J. and Tanner, M. E. 2007. Mechanistic studies on norcoclaurine synthase of benzylisoquinoline alkaloid biosynthesis:  An enzymatic Pictet-Spengler reaction. Biochemistry 46(35), pp. 10153-10161. (10.1021/bi700752n)