![Eddie Wang](https://profiles-cardiff.imgix.net/attachments/2634?w=200&h=200&auto=format&crop=faces&fit=crop&q=90")
Trosolwyg
I am a Reader within the 'Viral Immunology' group of the Division of Infection & Immunity, School of Medicine, College of Biomedical & Life Sciences. My research covers the biology and cellular immunology relating to Human Cytomegalovirus (HCMV) and the biological function of Death Receptor 3, a member of the TNFR superfamily involved in control of inflammatory and autoimmune disease. More details of the HCMV work can be seen at the Viral Immunology page.
Further details of publications can be found on Researchgate (https://www.researchgate.net/profile/Eddie_Wang) and Google Scholar (https://scholar.google.co.uk/citations?hl=en&user=DgvpthYAAAAJ)
Professional Membership
- British Society of Immunology
University Committees
- Human Tissue Officer for the Institute of Infection & Immunity
- Member of the Cardiff University HTA Research Governance Committee
- Panel Chair for PhD appraisals within the Institute of Infection & Immunity
- Member of the Systems Immunity University Research Institute (SIURI)
Cyhoeddiad
2024
- Litvin, U., Wang, E. C., Stanton, R. J., Fielding, C. A. and Hughes, J. 2024. Evolution of the cytomegalovirus RL11 gene family in old world monkeys and great apes. Virus Evolution 10(1), article number: veae066. (10.1093/ve/veae066)
- Kite, J., Hill, M., Preston, N., Rubina, A., Kollnberger, S., Wang, E. C. Y. and Elliott, G. 2024. Downregulation of endogenous nectin1 in human keratinocytes by herpes simplex virus 1 glycoprotein D excludes superinfection but does not affect NK cell function. Journal of General Virology 105(3) (10.1099/jgv.0.001969)
2023
- Vlachava, V. et al. 2023. HCMV-secreted glycoprotein gpUL4 inhibits TRAIL-mediated apoptosis and NK cell activation. Proceedings of the National Academy of Sciences 120(49), article number: e2309077120. (10.1073/pnas.2309077120)
- Rubina, A. et al. 2023. ADAM17 targeting by human cytomegalovirus remodels the cell surface proteome to simultaneously regulate multiple immune pathways. Proceedings of the National Academy of Sciences 120(33), article number: e2303155120. (10.1073/pnas.2303155120)
- Grant, M. D. et al. 2023. Combined anti-S1 and anti-S2 antibodies from hybrid immunity elicit potent cross-variant ADCC against SARS-CoV-2. JCI Insight 8(15), article number: e170681. (10.1172/jci.insight.170681)
- Luo, S., Liman, N., Li, C., Crossman, A., Wang, E. C. Y., Meylan, F. and Park, J. 2023. The cytokine receptor DR3 identifies and promotes the activation of thymic NKT17 cells. Cellular and Molecular Life Sciences 80(3), pp. 1-12., article number: 76. (10.1007/s00018-023-04726-7)
2022
- Morgan, H. J. et al. 2022. CD200 ectodomain shedding into the tumor microenvironment leads to NK cell dysfunction and apoptosis. Journal of Clinical Investigation 132(21), article number: e150750. (10.1172/JCI150750)
- Statkute, E., Wang, E. and Stanton, R. 2022. An optimised CRISPR/Cas9 adenovirus vector (AdZ-CRISPR) for high throughput cloning of sgRNA, using enhanced sgRNA and Cas9 variants. Human Gene Therapy 33(17-18), pp. 990-1001. (10.1089/hum.2021.120)
- Crozier, T. W. et al. 2022. Quantitative proteomic analysis of SARS-CoV-2 infection of primary human airway ciliated cells and lung epithelial cells demonstrates the effectiveness of SARS-CoV-2 innate immune evasion. Wellcome Open Research 7, article number: 224. (10.12688/wellcomeopenres.17946.1)
- Fielding, C. A. et al. 2022. SARS-CoV-2 host-shutoff impacts innate NK cell functions, but antibody-dependent NK activity is strongly activated through non-spike antibodies. eLife 11 (10.7554/eLife.74489)
- Wang, E. C., Fielding, C. A. and Stanton, R. J. 2022. Concerns over functional experiments, interpretation, and required controls. Journal of Clinical Investigation 132(5), article number: e157369. (10.1172/JCI157369)
- Nightingale, K. et al. 2022. Human cytomegalovirus protein RL1 degrades the antiviral factor SLFN11 via recruitment of the CRL4 E3 ubiquitin ligase complex. Proceedings of the National Academy of Sciences 119(6), article number: e2108173119. (10.1073/pnas.2108173119)
2021
- Wickenhagen, A. et al. 2021. A prenylated dsRNA sensor protects against severe COVID-19. Science 374(6567), pp. 535-536. (10.1126/science.abj3624)
- Vlachava, V. et al. 2021. Monoclonal antibodies targeting nonstructural viral antigens can activate ADCC against human cytomegalovirus. Journal of Clinical Investigation 131(4), article number: e139296. (10.1172/JCI139296)
2020
- Ahmed, R. et al. 2020. CD57+ Memory T Cells Proliferate In Vivo. Cell Reports 33(11), article number: 108501. (10.1016/j.celrep.2020.108501)
- Lau, B. et al. 2020. Human Cytomegalovirus long non-coding RNA1.2 suppresses extracellular release of the pro-inflammatory Cytokine IL-6 by blocking NF-κB activation. Frontiers in Cellular and Infection Microbiology 10, article number: 361. (10.3389/fcimb.2020.00361)
- Elasifer, H. et al. 2020. Downregulation of HLA-I by the molluscum contagiosum virus mc080 impacts NK-cell recognition and promotes CD8+ T-cell evasion. Journal of General Virology 101, pp. 863-872. (10.1099/jgv.0.001417)
2019
- Collins, F. L., Stone, M. D., Turton, J., McCabe, L. R., Wang, E. C. and Williams, A. S. 2019. Oestrogen-deficiency induces bone loss by modulating CD14+ monocyte and CD4+ T cell DR3 expression and serum TL1A levels. BMC Musculoskeletal Disorders 20(1), article number: 326. (10.1186/s12891-019-2704-z)
- Nobre, L. V. et al. 2019. Human cytomegalovirus interactome analysis identifies degradation hubs, domain associations and viral protein functions. eLife 8, article number: e49894. (10.7554/eLife.49894)
2018
- Boelen, L. et al. 2018. Inhibitory killer cell immunoglobulin-like receptors strengthen CD8+ T cell-mediated control of HIV-1, HCV, and HTLV-1. Science Immunology 3(29), article number: eaao2892. (10.1126/sciimmunol.aao2892)
- Patel, M., Vlachava, V., Forbes, S. K., Fielding, C. A., Stanton, R. J. and Wang, E. 2018. HCMV-encoded NK modulators: Lessons from in vitro and in vivo genetic variation. Frontiers in Immunology 9, article number: 2214. (10.3389/fimmu.2018.02214)
- Nightingale, K. et al. 2018. High-definition analysis of host protein stability during human cytomegalovirus infection reveals antiviral factors and viral evasion mechanisms. Cell Host and Microbe 24(3), pp. 447-460.e11. (10.1016/j.chom.2018.07.011)
- Wang, E. C. Y. et al. 2018. Suppression of costimulation by human cytomegalovirus promotes evasion of cellular immune defenses. Proceedings of the National Academy of Sciences 115(19), pp. 4998-5003. (10.1073/pnas.1720950115)
- Malhotra, N. et al. 2018. RORα-expressing T regulatory cells restrain allergic skin inflammation. Science Immunology 3(21), article number: eaao6923. (10.1126/sciimmunol.aao6923)
2017
- Singh, R. et al. 2017. Death receptor 3 regulates distinct pathological attributes of acute versus chronic murine allergic lung inflammation. Cellular Immunology 320, pp. 62-70. (10.1016/j.cellimm.2017.09.005)
- Collins, F. L. et al. 2017. CCL3 and MMP-9 are induced by TL1A during death receptor 3 (TNFRSF25)-dependent osteoclast function and systemic bone loss. Bone 97, pp. 94-104. (10.1016/j.bone.2017.01.002)
2016
- Williams, A., Wang, E. C. Y., Thurner, L. and Liu, C. 2016. Novel insights into TNF receptor, DR3 and progranulin pathways in arthritis and bone remodeling. Arthritis & Rheumatology 68(12), pp. 2845-2856. (10.1002/art.39816)
- Perks, W. V. et al. 2016. Death Receptor 3 promotes chemokine directed leukocyte recruitment in acute resolving inflammation and is essential for pathological development of mesothelial fibrosis in chronic disease. American Journal of Pathology 186(11), pp. 2813-2823. (10.1016/j.ajpath.2016.07.021)
- Jia, L., Bamias, G., Burkly, L. C., Wang, E. C. Y., Gruszka, D., Pizarro, T. T. and Cominelli, F. 2016. A novel role for TL1A/DR3 in protection against intestinal injury and infection. Journal of Immunology 197(1), pp. 377-386. (10.4049/jimmunol.1502466)
- Williams, J., Wang, E. C. Y., Lang, D. and Williams, A. S. 2016. Characterisation of Death Receptor 3 dependent aortic changes during inflammatory arthritis. Pharmacology Research & Perspectives 4(4), article number: e0024. (10.1002/prp2.240)
2015
- Wilkinson, G. W. G. et al. 2015. Human cytomegalovirus: taking the strain. Medical Microbiology and Immunology 204(3), pp. 273-284. (10.1007/s00430-015-0411-4)
- Collins, F. L., Williams, J., Bloom, A. C., Stone, M. D., Choy, E. H. S., Wang, E. C. Y. and Williams, A. S. 2015. Death Receptor 3 (TNFRSF25) Increases mineral apposition by osteoblasts and region specific new bone formation in the axial skeleton of male DBA/1 mice. Journal of Immunology Research 2015, pp. -., article number: 901679. (10.1155/2015/901679)
2014
- Wang, E. C. Y. et al. 2014. Regulation of early cartilage destruction in inflammatory arthritis by death receptor 3. Arthritis and Rheumatology 66(10), pp. 2762-2772. (10.1002/art.38770)
- Stanton, R. J. et al. 2014. HCMV pUL135 remodels the actin cytoskeleton to impair immune recognition of infected cells. Cell Host and Microbe 16(2), pp. 201-214. (10.1016/j.chom.2014.07.005)
- Weekes, M. et al. 2014. Quantitative temporal viromics: an approach to investigate host-pathogen interaction. Cell 157(6), pp. 1460-1472. (10.1016/j.cell.2014.04.028)
- Fielding, C. A. et al. 2014. Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation. PLoS Pathogens 10(5), article number: e1004058. (10.1371/journal.ppat.1004058)
2013
- Pembroke, T., Christian, A., Jones, E., Hills, R. K., Wang, E. C. Y., Gallimore, A. M. and Godkin, A. J. 2013. The paradox of NKp46+ natural killer cells: drivers of severe hepatitis C virus-induced pathology but in-vivo resistance to interferon treatment. Gut n/a (10.1136/gutjnl-2013-304472)
- Smith, W. et al. 2013. Human cytomegalovirus glycoprotein UL141 targets the TRAIL death receptors to thwart host innate antiviral defenses. Cell Host & Microbe 13(3), pp. 324-335. (10.1016/j.chom.2013.02.003)
2012
- Collins, F., Stone, M. D., Goodfellow, R. M., Choy, E. H. S., Wang, E. C. Y. and Williams, A. S. 2012. TNF-like protein 1A/death receptor 3 pathway regulates osteoclastogenesis and is associated with erosive disease in rheumatoid arthritis. Arthritis and Rheumatism 64(10), pp. S768-S768.
- Coles, S., Hills, R. K., Wang, E. C. Y., Burnett, A. K., Man, S. T., Darley, R. L. and Tonks, A. 2012. Increased CD200 expression in acute myeloid leukemia is linked with an increased frequency of FoxP3+ regulatory T cells [Letter]. Leukemia 26(9), pp. 2146-2148. (10.1038/leu.2012.75)
- Wang, E. C. Y. 2012. On death receptor 3 and its ligands... Immunology 137(1), pp. 114-116. (10.1111/j.1365-2567.2012.03606.x)
- Pembroke, T. et al. 2012. Rapid early innate control of hepatitis C virus during IFN-α treatment compromises adaptive CD4+T-cell immunity. European Journal of Immunology 42(9), pp. 2383-2394. (10.1002/eji.201142072)
- Coles, S., Hills, R. K., Wang, E. C. Y., Burnett, A. K., Man, S. T., Darley, R. L. and Tonks, A. 2012. Expression of CD200 on AML blasts directly suppresses memory T-cell function [Letter]. Leukemia 26(9), pp. 2148-2151. (10.1038/leu.2012.77)
- Twohig, J. P. et al. 2012. The death receptor 3/TL1A pathway is essential for efficient development of antiviral CD4+ and CD8+ T-cell immunity. The FASEB Journal 26(8), pp. 3575-3586. (10.1096/fj.11-200618)
- Pembroke, T., Wang, E. C. Y., Gallimore, A. M. and Godkin, A. J. 2012. PMO-174 Intrahepatic natural killer cell phenotyping and functional analysis by fine needle aspiration in chronic HCV infection. Gut 61(S2), pp. A144-A145. (10.1136/gutjnl-2012-302514b.174)
- Collins, F., Stone, M., Wang, E. C. Y. and Williams, A. S. 2012. Death receptor 3 and tl1a: A regulatory pathway of bone turnover and target for osteoporosis therapy? [Abstract]. Osteoporosis International 23, pp. S528-S528.
- Calder, C. J. and Wang, E. C. Y. 2012. An essential role for death receptor 3 in experimental autoimmune uveoretinitis [Letter]. Ocular Immunology and Inflammation 20(3), pp. 212-214. (10.3109/09273948.2012.658135)
- Collins, F., Stone, M. D., Wang, E. C. Y. and Williams, A. S. 2012. Death receptor 3 and Tl1a: A driving force in osteoclast differentiation and potential target for rheumatoid arthritis therapy [Abstract]. Rheumatology 51(s3), pp. 140-140. (10.1093/rheumatology/kes108)
- Al-Lamki, R. S., Lu, W., Finlay, S., Twohig, J. P., Wang, E. C. Y., Tolkovsky, A. M. and Bradley, J. R. 2012. DR3 signaling protects against cisplatin nephrotoxicity mediated by tumor necrosis factor. American Journal of Pathology 180(4), pp. 1454-1464. (10.1016/j.ajpath.2012.01.003)
- Prod'homme, V. et al. 2012. Human cytomegalovirus UL40 signal peptide regulates cell surface expression of the NK cell ligands HLA-E and gpUL18. The Journal of Immunology 188(6), pp. 2794-2804. (10.4049/jimmunol.1102068)
2011
- Wang, E. C. Y. et al. 2011. Control of early cartilage destruction in inflammatory arthritis by death receptor 3 [Abstract]. Immunology 135(s1), pp. 153-153. (10.1111/j.1365-2567.2011.03534.x)
- Coles, S., Man, S. T., Hills, R. K., Wang, E. C. Y., Burnett, A. K., Darley, R. L. and Tonks, A. 2011. CD200 inhibits memory Th1 cell function in acute myeloid leukaemia (AML)[Abstract]. Immunology 135(S1), pp. 178. (10.1111/j.1365-2567.2011.03534.x)
- Stacey, M. A., Marsden, M., Wang, E. C. Y., Wilkinson, G. W. G. and Humphreys, I. R. 2011. IL-10 restricts activation-induced death of NK cells during acute murine cytomegalovirus infection. The Journal of Immunology 187(6), pp. 2944-2952. (10.4049/jimmunol.1101021)
- Coles, S., Wang, E. C. Y., Man, S. T., Hills, R. K., Burnett, A. K., Tonks, A. and Darley, R. L. 2011. CD200 expression suppresses natural killer cell function and directly inhibits patient anti-tumor response in acute myeloid leukemia. Leukemia 25(5), pp. 792-799. (10.1038/leu.2011.1)
- Jones, G. W. et al. 2011. Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation. The FASEB Journal 25(1), pp. 409-419. (10.1096/fj.10-166843)
- Wang, E. C. Y. 2011. CD4+ T cells, human cytomegalovirus and end-stage renal disease. Nephrology Dialysis Transplantation 26(5), pp. 1467-1470. (10.1093/ndt/gfr167)
2010
- Jones, G. W. et al. 2010. Differential responsiveness of naive and activated T cells to TL1A that affect Th17 generation, maintenance and proliferation [Abstract]. Immunology 131(s1), pp. 102-102. (10.1111/j.1365-2567.2010.03390.x)
- Williams, A. S., Collins, F., Newton, Z., Goodfellow, R. M., Rajak, R., Calder, C. J. and Wang, E. C. Y. 2010. Death Receptor 3 orchestrates erosive pathology in inflammatory arthritis [Poster Abstract]. Immunology 131(s1), pp. 53-53. (10.1111/j.1365-2567.2010.03390.x)
- Coles, S., Man, S. T., Hills, R. K., Wang, E. C. Y., Burnett, A. K., Darley, R. L. and Tonks, A. 2010. Over-expression of CD200 un acute myeloid leukemia mediates the expansion of regulatory T-lymphocytes and directly inhibits natural killer cell tumor immunity [Abstract]. Blood 116(21), pp. 218-218.
- McLaren, J. E. et al. 2010. The TNF-like protein 1A-death receptor 3 pathway promotes macrophage foam cell formation in vitro. Journal of Immunology 184(10), pp. 5827-5834. (10.4049/jimmunol.0903782)
- Twohig, J. P. et al. 2010. Age-dependent maintenance of motor controland corticostriatal innervation by death receptor 3. Journal of Neuroscience 30(10), pp. 3782-3792. (10.1523/JNEUROSCI.1928-09.2010)
- Taraban, V. et al. 2010. Sustained TL1A expression modulates effector and regulatory T-cell responses and drives intestinal goblet cell hyperplasia. Mucosal Immunology 4(2), pp. 186-196. (10.1038/mi.2010.70)
2009
- Williams, A. S. et al. 2009. A functional role for death receptor-3 in arthritis [Abstract]. Cytokine 48(1-2), pp. 134-134. (10.1016/j.cyto.2009.07.568)
- Twohig, J. P. et al. 2009. Defective B cell ontogeny and humoral immune response in mice prematurely expressing human complement receptor 2 (CR2, CD21) is similar to that seen in aging wild type mice. Molecular Immunology 46(10), pp. 2002-2013. (10.1016/j.molimm.2009.03.007)
- Williams, A. S. et al. 2009. Functional role of dr3 in inflammatory arthritis: A novel target for therapy? [Abstract]. Rheumatology 48(s1), pp. I8-I8. (10.1093/rheumatology/kep704)
- Newton, Z., Bull, M., Wang, E. C. Y. and Williams, A. S. 2009. The role of dr3/tl1a in cartilage depletion caused by inflammatory arthritis [Abstract]. Rhuematology 48(s1), pp. I30-I30. (10.1093/rheumatology/kep713)
2008
- Jones, G. W. et al. 2008. TL1A/TNFSF15 promotes a STAT1-mediated suppression of IL-17-secreting T-helper cells [Abstract]. Immunology 125(s1), pp. 62-62. (10.1111/j.1365-2567.2008.02974.x)
- Williams, A. S. et al. 2008. The role of death receptor 3 in inflammatory arthritis [Abstract]. Immunology 125, pp. 34-34. (10.1111/j.1365-2567.2008.02974.x)
- Bull, M. J. et al. 2008. The Death Receptor 3-TNF-like protein 1A pathway drives adverse bone pathology in inflammatory arthritis. Journal of Experimental Medicine 205(11), pp. 2457-2464. (10.1084/jem.20072378)
- Meylan, F. et al. 2008. The TNF-family receptor DR3 is essential for diverse T cell-mediated inflammatory diseases. Immunity 29(1), pp. 79-89. (10.1016/j.immuni.2008.04.021)
- Al-Lamki, R. S. et al. 2008. TL1A both promotes and protects from renal inflammation and injury. Journal of the American Society of Nephrology 19(5), pp. 953-960. (10.1681/ASN.2007060706)
- McSharry, B. P. et al. 2008. Adenovirus E3/19K promotes evasion of NK cell recognition by intracellular sequestration of the NKG2D ligands major histocompatibility complex class I chain-related proteins A and B. Journal of Virology 82(9), pp. 4585-4594. (10.1128/JVI.02251-07)
- Jones, G. W. et al. 2008. TL1A negatively regulates development of T helper 17 cells [Abstract]. Rheumatology 47(S2), pp. II20-II20. (10.1093/rheumatology/kem509)
- Chong, L. K., Aicheler, R., Llewellyn-Lacey, S., Tomasec, P., Brennan, P. and Wang, E. C. Y. 2008. Proliferation and interleukin 5 production by CD8hiCD57+ T cells. European Journal of Immunology 38(4), pp. 995-1000. (10.1002/eji.200737687)
- Wilkinson, G. W. G. et al. 2008. Modulation of natural killer cells by human cytomegalovirus. Journal of Clinical Virology 41(3), pp. 206-212. (10.1016/j.jcv.2007.10.027)
2007
- Stanton, R. J., McSharry, B. P., Rickards, C. R., Wang, E. C. Y., Tomasec, P. and Wilkinson, G. W. G. 2007. Cytomegalovirus destruction of focal adhesions revealed in a high-throughput Western blot analysis of cellular protein expression. Journal of Virology 81(15), pp. 7860-7872. (10.1128/JVI.02247-06)
- Jones, G. W. et al. 2007. TL1A counteracts the IL-6 control of the STAT3-mediated development of T helper 17 cells [Abstract]. Cytokine 39(1), pp. 18-19.
- Prod'homme, V. et al. 2007. The Human Cytomegalovirus MHC Class I Homolog UL18 Inhibits LIR-1+ but Activates LIR-1- NK Cells. The Journal of Immunology 178(7), pp. 4473-4481.
2006
- Omidvar, N., Wang, E. C. Y., Brennan, P., Longhi, M. P., Smith, R. A. G. and Morgan, B. P. 2006. Expression of glycosylphosphatidylinositol-anchored CD59 on target cells enhances human NK cell-mediated cytotoxicity. The Journal of Immunology 176(5), pp. 2915-2923. (10.4049/jimmunol.176.5.2915)
- Omidvar, N., Wang, E. C. Y., Brennan, P., Longhi, M. P., Smith, R. A. G. and Morgan, B. P. 2006. Expression of glycosylphosphatidylinositol-anchored CD59 on target cells enhances human NK cell-mediated cytotoxicity. Journal of immunology 176(5), pp. 2915-2923.
2005
- Griffin, C., Wang, E. C. Y., McSharry, B. P., Rickards, C. R., Browne, H., Wilkinson, G. W. G. and Tomasec, P. 2005. Characterization of a highly glycosylated form of the human cytomegalovirus HLA class I homologue gpUL18. Journal of General Virology 86(11), pp. 2999-3008. (10.1099/vir.0.81126-0)
- Siebert, S., Amos, N., Fielding, C. A., Wang, E. C. Y., Aksentijevich, I., Williams, B. D. and Brennan, P. 2005. Reduced tumor necrosis factor signaling in primary human fibroblasts containing a tumor necrosis factor receptor superfamily 1A mutant. Arthritis & Rheumatism 52(4), pp. 1287-1282. (10.1002/art.20955)
- Tomasec, P. et al. 2005. Downregulation of natural killer cell-activating ligand CD155 by human cytomegalovirus UL141. Nature Immunology 6(2), pp. 181-188. (10.1038/ni1156)
- Vedhara, K. and Wang, E. C. Y. 2005. Assessment of the immune system in human psychoneuroimmunology. In: Vedhara, K. and Irwin, M. R. eds. Human Psychneuroimmunology. Oxford: Oxford University Press, pp. 53-80.
2002
- Wang, E. C. Y. et al. 2002. UL40-mediated NK evasion during productive infection with human cytomegalovirus. Proceedings of the National Academy of Sciences 99(11), pp. 7570-7575. (10.1073/pnas.112680099)
2001
- Wang, E. C. Y., Thern, A., Denzel, A., Kitson, J., Farrow, S. N. and Owen, M. J. 2001. DR3 regulates negative selection during thymocyte development. Molecular and cellular biology 21(10), pp. 3451-61. (10.1128/MCB.21.10.3451-3461.2001)
- Wang, E. C. Y., Kitson, J., Thern, A., Williamson, J., Farrow, S. N. and Owen, M. J. 2001. Genomic structure, expression, and chromosome mapping of the mouse homologue for the WSL-1 (DR3, Apo3, TRAMP, LARD, TR3, TNFRSF12) gene. Immunogenetics 53(1), pp. 59-63. (10.1007/s002510000290)
- Lawson, T. et al. 2001. Functional differences between influenza A-specific cytotoxic T lymphocyte clones expressing dominant and subdominant TCR. International Immunology 13(11), pp. 1383-1390. (10.1093/intimm/13.11.1383)
1998
- Wang, E. C. Y. 1998. Sorting of human peripheral blood T-cell subsets using immunomagnetic beads. In: Pound, J. D. ed. Immunochemical Protocols., Vol. 80. Methods in Molecular Biology Vol. 2. Humana Press, pp. 365-376., (10.1007/978-1-59259-257-9_37)
1992
- Wang, E. C. Y., Borysiewicz, L. K. and Weetman, A. P. 1992. Cell sorting using immunomagnetic beads. In: Manson, M. M. ed. Immunochemical Protocols. Methods in Molecular Biology Vol. 10. Totowa, NJ: Humana Press, pp. 347-358.
Articles
- Litvin, U., Wang, E. C., Stanton, R. J., Fielding, C. A. and Hughes, J. 2024. Evolution of the cytomegalovirus RL11 gene family in old world monkeys and great apes. Virus Evolution 10(1), article number: veae066. (10.1093/ve/veae066)
- Kite, J., Hill, M., Preston, N., Rubina, A., Kollnberger, S., Wang, E. C. Y. and Elliott, G. 2024. Downregulation of endogenous nectin1 in human keratinocytes by herpes simplex virus 1 glycoprotein D excludes superinfection but does not affect NK cell function. Journal of General Virology 105(3) (10.1099/jgv.0.001969)
- Vlachava, V. et al. 2023. HCMV-secreted glycoprotein gpUL4 inhibits TRAIL-mediated apoptosis and NK cell activation. Proceedings of the National Academy of Sciences 120(49), article number: e2309077120. (10.1073/pnas.2309077120)
- Rubina, A. et al. 2023. ADAM17 targeting by human cytomegalovirus remodels the cell surface proteome to simultaneously regulate multiple immune pathways. Proceedings of the National Academy of Sciences 120(33), article number: e2303155120. (10.1073/pnas.2303155120)
- Grant, M. D. et al. 2023. Combined anti-S1 and anti-S2 antibodies from hybrid immunity elicit potent cross-variant ADCC against SARS-CoV-2. JCI Insight 8(15), article number: e170681. (10.1172/jci.insight.170681)
- Luo, S., Liman, N., Li, C., Crossman, A., Wang, E. C. Y., Meylan, F. and Park, J. 2023. The cytokine receptor DR3 identifies and promotes the activation of thymic NKT17 cells. Cellular and Molecular Life Sciences 80(3), pp. 1-12., article number: 76. (10.1007/s00018-023-04726-7)
- Morgan, H. J. et al. 2022. CD200 ectodomain shedding into the tumor microenvironment leads to NK cell dysfunction and apoptosis. Journal of Clinical Investigation 132(21), article number: e150750. (10.1172/JCI150750)
- Statkute, E., Wang, E. and Stanton, R. 2022. An optimised CRISPR/Cas9 adenovirus vector (AdZ-CRISPR) for high throughput cloning of sgRNA, using enhanced sgRNA and Cas9 variants. Human Gene Therapy 33(17-18), pp. 990-1001. (10.1089/hum.2021.120)
- Crozier, T. W. et al. 2022. Quantitative proteomic analysis of SARS-CoV-2 infection of primary human airway ciliated cells and lung epithelial cells demonstrates the effectiveness of SARS-CoV-2 innate immune evasion. Wellcome Open Research 7, article number: 224. (10.12688/wellcomeopenres.17946.1)
- Fielding, C. A. et al. 2022. SARS-CoV-2 host-shutoff impacts innate NK cell functions, but antibody-dependent NK activity is strongly activated through non-spike antibodies. eLife 11 (10.7554/eLife.74489)
- Wang, E. C., Fielding, C. A. and Stanton, R. J. 2022. Concerns over functional experiments, interpretation, and required controls. Journal of Clinical Investigation 132(5), article number: e157369. (10.1172/JCI157369)
- Nightingale, K. et al. 2022. Human cytomegalovirus protein RL1 degrades the antiviral factor SLFN11 via recruitment of the CRL4 E3 ubiquitin ligase complex. Proceedings of the National Academy of Sciences 119(6), article number: e2108173119. (10.1073/pnas.2108173119)
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- Coles, S., Wang, E. C. Y., Man, S. T., Hills, R. K., Burnett, A. K., Tonks, A. and Darley, R. L. 2011. CD200 expression suppresses natural killer cell function and directly inhibits patient anti-tumor response in acute myeloid leukemia. Leukemia 25(5), pp. 792-799. (10.1038/leu.2011.1)
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- Wang, E. C. Y. 2011. CD4+ T cells, human cytomegalovirus and end-stage renal disease. Nephrology Dialysis Transplantation 26(5), pp. 1467-1470. (10.1093/ndt/gfr167)
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- Coles, S., Man, S. T., Hills, R. K., Wang, E. C. Y., Burnett, A. K., Darley, R. L. and Tonks, A. 2010. Over-expression of CD200 un acute myeloid leukemia mediates the expansion of regulatory T-lymphocytes and directly inhibits natural killer cell tumor immunity [Abstract]. Blood 116(21), pp. 218-218.
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- Twohig, J. P. et al. 2009. Defective B cell ontogeny and humoral immune response in mice prematurely expressing human complement receptor 2 (CR2, CD21) is similar to that seen in aging wild type mice. Molecular Immunology 46(10), pp. 2002-2013. (10.1016/j.molimm.2009.03.007)
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- Newton, Z., Bull, M., Wang, E. C. Y. and Williams, A. S. 2009. The role of dr3/tl1a in cartilage depletion caused by inflammatory arthritis [Abstract]. Rhuematology 48(s1), pp. I30-I30. (10.1093/rheumatology/kep713)
- Jones, G. W. et al. 2008. TL1A/TNFSF15 promotes a STAT1-mediated suppression of IL-17-secreting T-helper cells [Abstract]. Immunology 125(s1), pp. 62-62. (10.1111/j.1365-2567.2008.02974.x)
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- Bull, M. J. et al. 2008. The Death Receptor 3-TNF-like protein 1A pathway drives adverse bone pathology in inflammatory arthritis. Journal of Experimental Medicine 205(11), pp. 2457-2464. (10.1084/jem.20072378)
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Book sections
- Vedhara, K. and Wang, E. C. Y. 2005. Assessment of the immune system in human psychoneuroimmunology. In: Vedhara, K. and Irwin, M. R. eds. Human Psychneuroimmunology. Oxford: Oxford University Press, pp. 53-80.
- Wang, E. C. Y. 1998. Sorting of human peripheral blood T-cell subsets using immunomagnetic beads. In: Pound, J. D. ed. Immunochemical Protocols., Vol. 80. Methods in Molecular Biology Vol. 2. Humana Press, pp. 365-376., (10.1007/978-1-59259-257-9_37)
- Wang, E. C. Y., Borysiewicz, L. K. and Weetman, A. P. 1992. Cell sorting using immunomagnetic beads. In: Manson, M. M. ed. Immunochemical Protocols. Methods in Molecular Biology Vol. 10. Totowa, NJ: Humana Press, pp. 347-358.
Ymchwil
THEMÂU YMCHWIL
Rwyf wedi bod â diddordeb ers amser maith mewn cytomegalofirws dynol (HCMV), firws herpesaidd sy'n brif achos heintus abnormaleddau cynhenid ac sy'n achosi clefyd sy'n bygwth bywyd mewn derbynwyr trawsblaniadau a chleifion AIDS sydd wedi'u hatal rhag imiwnoimiwnedd. Mae HCMV yn heintio am fywyd ac argraffnodau ar y system imiwnedd; Adroddwyd yn wreiddiol ar natur oligoclonal CD8 + CD57+ T ehangiadau celloedd sy'n gysylltiedig â haint HCMV dros 25 mlynedd yn ôl (1-3). Rwyf wedi gwneud cyfraniad sylweddol at ein dealltwriaeth o osgoi imiwnedd HCMV gan gynnal nifer o grantiau ymchwil MRC a rhaglen Ymddiriedolaeth Wellcome i astudio hyn ers hynny. Peter Tomasec, Gavin Wilkinson a minnau oedd y cyntaf i ddiffinio swyddogaeth osgoi firws NK yn llawn (HCMV UL40) (4, 5). Wrth archwilio'r gwahaniaethau rhwng straen HCMV labordy a chlinigol gyda Richard Stanton, gwnaethom nodi ymhellach UL135, UL141, UL142 (gyda Mark Wills, Caergrawnt) a UL148 fel swyddogaethau osgoi newydd NK (6-9). Wrth ail-werthuso'r homolog HLA-I UL18, gwnaethom ddangos bod UL18 yn darparu ar gyfer ataliad NK sy'n dibynnu ar LIR1 (10) ac yn nodi mai UD 18 a US 20 yw'r imiwnogau HCMV amlycaf sy'n gyfrifol am atal y gell NK sy'n actifadu ligands MICA a B7-H6 (11, 12). Mae'r ymchwil ddiweddaraf wedi dangos bod ADAM17 yn targedu gan UL148 ac mae UL148D yn newid proteome wyneb celloedd heintiedig yn sylweddol ac yn atal celloedd NK yn anuniongyrchol (13), tra bod y blociau protein UL4 hydawdd TRAIL-gyfryngu sefydlu marwolaeth celloedd ac actifadu NK (14). Mae hyn yn cynrychioli ~ hanner yr holl atalyddion NK wedi'u hamgodio HCMV hysbys (15, 16). Arweiniodd fy hanes o osgoi imiwnedd at ei gynnwys fel arweinydd ar y cyd Caerdydd ar gyfer thema Osgoi Imiwnedd ar gyfer Consortiwm Imiwnoleg Coronafeirws y DU (DU-CIC) sy'n astudio imiwnopatholeg SARS-CoV-2.
Rwyf hefyd wedi gwneud cyfraniad sylweddol i faes ymchwil Derbynnydd Marwolaeth 3 (DR3). Mae DR3 yn aelod o Superfamily Receptor Ffactor Tiwmor Necrosis sy'n ymwneud â rheoleiddio imiwnedd. Cynhyrchais yr unig lygoden DR3ko hysbys (17), gan sefydlu swyddogaeth hanfodol DR3 mewn clefydau llidiol / awtoimiwn yn amrywio o arthritis llidiol (18) i glefyd llidiol y coluddyn (19) ac imiwnedd gwrthfeirysol (20) trwy reoli celloedd-T (21, 22) a swyddogaeth myeloid (18, 23).
CYLLID CYFREDOL (grantiau mawr yn unig)
2023-2030 Gwobr Darganfod Ymddiriedolaeth Wellcome "Manteisio ar ymatebion imiwn gwrthfeirysol newydd" a ddyfarnwyd i R Stanton (PI), E Wang (£1,976,186)
2023-2027 Astudiaeth GW4-PhD "Cynhyrchu celloedd lladd ar gyfer imiwnotherapi yn erbyn canser a pathogenau" a ddyfernir i E Wang (PI), M Heinrich-Sevcenco, R Stanton, L Wooldridge (Bryste) (£114,536)
2022-2025 Grant Prosiect KRUK " Nodi ffactorau risg mewn cleifion trawsblaniad arennau ar gyfer clefyd a achosir gan haint cytomegalofirws dynol" a ddyfarnwyd i E Wang (PI), R Stanton, S Griffin (£209,986)
2021-2026 Technegydd 5 mlynedd wedi'i ariannu drwy gydweithio ar SIA Ymddiriedolaeth Wellcome a ddyfernir i Ben Willcox (Prifysgol Birmingham) "Archwilio paradeimau cynhenid tebyg ac addasol gamma delta celloedd T mewn iechyd a chlefyd" (~£2m, £379,510 ohono i Gaerdydd)
Grant Prosiect MRC 2020-2024 "Nodweddu dosbarth newydd o imiwnofasinau celloedd lladdwr naturiol" a ddyfarnwyd i E Wang (PI), R Stanton, D Price, S Kollnberger (£819,703)
CYLLID HANESYDDOL (grantiau mawr yn unig)
Grant Prosiect NIHR 2021-2023 "Penderfynyddion imiwnolegol a firolegol COVID hir" a ddyfarnwyd i D Price (PI), H Davies, I Humphreys, P Morgan, R Stanton, E Wang (£744,457)
Grant Prosiect MRC 2019-2023 "Rôl gwrthgyrff wrth alluogi rheolaeth gyfryngol celloedd o haint HCMV" a ddyfarnwyd i R Stanton (PI), E Wang (£762,492)
Grant Cydweithio Ymddiriedolaeth Wellcome 2017-2023 "Dadansoddiad o bathogenesisis cytomegalofirws mewn system her ddynol " a ddyfarnwyd i P Griffiths (Coleg Prifysgol Llundain). Enwodd Wang yn gydweithredwr (~ £1.7m, £329,728 ohono i Gaerdydd ar gyfer PDRA)
Grant Ymchwil MRC 2020-2021 "Llwyfan sylfaenol yn y DU i astudio imiwnoleg ac imiwnopatholeg COVID-19: Consortiwm Imiwnoleg Coronafeirws y DU" a ddyfarnwyd i P Moss (Prifysgol Birmingham) a 73 Cyd-ymchwilwyr eraill ledled y DU gan gynnwys E Wang (~ £8m, £300,000 ohono i Gaerdydd am 3 swydd mewn labordai gwahanol)
Grant Prosiect MRC 2017-2021 "Nodweddiad systematig o barth pathogenicity HCMV a gollwyd yn ddigymell o straen passage a ddefnyddir yn gyffredin" a ddyfarnwyd i E Wang (PI - unig ddeiliwr), G Wilkinson (wedi ymddeol), P Tomasec (ymadawedig) (£956,196)
2010-2018 Grant Rhaglen Ymddiriedolaeth Wellcome "Modiwleiddio imiwnedd cynnal gan cytomegalofirws dynol" a ddyfarnwyd i G Wilkinson (PI), E Wang, P Tomasec (£1,062,032)
Grant Prosiect MRC 2014-2018 "Ymchwiliad systematig i swyddogaeth genynnau cytomegalofirws dynol" a ddyfarnwyd i G Wilkinson (PI), E Wang, P Tomasec (£679,426)
Grant Prosiect MRC 2010-2013 "Dadansoddiad o cytomegalofirws dynol math gwyllt" a ddyfarnwyd i G Wilkinson (PI), E Wang, P Tomasec (£901,468)
2009-2013 Grant Prosiect MRC "Ymchwilio modiwleiddio imiwnedd i haint firaol parhaus gan y derbynnydd marwolaeth 3/TNF tebyg i lwybr 1A" a ddyfarnwyd i E Wang (PI), I Humphreys (£467,078)
2007-2010 Grant Prosiect MRC "Modiwleiddio celloedd lladd naturiol gan cytomegalofirws dynol" a ddyfarnwyd i G Wilkinson (PI), E Wang, P Tomasec (£668,526)
2006-2009 Grant Cydweithio MRC "Adnodd imiwnoleg Cellog a firaol yng Ngholeg Meddygaeth Cymru, Prifysgol Caerdydd" a ddyfarnwyd i E Wang (PI), A Gallimore (£188,888)
2004-2009 Grant Sefydliad Gyrfa MRC "Rôl derbynnydd marwolaeth 3 mewn swyddogaeth celloedd CD8+ T ymylol" a ddyfarnwyd i E Wang (unig deiliad) (£643,596)
2004-2009 Grant Rhaglen Ymddiriedolaeth Wellcome "Modiwleiddio imiwnedd gan cytomegalofirws dynol" a ddyfarnwyd i G Wilkinson (PI), E Wang, P Tomasec (£789,117)
2004-2007 Grant Prosiect Ymddiriedolaeth Wellcome "Osgoi imiwnedd gan firws Epstein Barr" a ddyfarnwyd i M Rowe (PI), E Wang (£304,679)
2002-2005 Grant Prosiect Ymddiriedolaeth Wellcome "Cynhyrchu a swyddogaeth celloedd CD8+, CD57+ T a achosir gan haint HCMV" a ddyfarnwyd i E Wang (unig ddeiliwr) (£167,415)
1995-1998 Cymrodoriaeth Goffa Beit "Mecanweithiau moleciwlaidd datblygiad thymocyte" a ddyfarnwyd i E Wang (unig ddeiliwr) (~ £135,000)
CYFEIRNODAU
1. Wang EC et al, Clin Exp Immunol 94, 297 (1993); 2. Wang EC et al, J Immunol 155, 5046 (1995); 3. Chong LK et al, Eur J Immunol 38, 995 (2008); 4. Tomasec P et al, Gwyddoniaeth 287, 1031 (2000); 5. Wang EC et al, PNAS UDA 99, 7570 (2002); 6. Wills MR et al, J Immunol 175, 7457 (2005); 7. Wang EC et al, PNAS UDA 115, 4998 (2018); 8. Tomasec P, Wang EC et al, Natur Immunol 6, 181 (2005); 9. Stanton RJ et al, Cell Host Microbe 16, 201 (2014); 10. Prod'homme V et al, J Immunol 188, 2794 (2012); 11. Fielding et al, PLoS Pathog 10, e1004058 (2014); 12. Fielding CA et al, Elife 6, e22206 (2017); 13. Rubina A et al, PNAS UDA 120, e2303155120 (2023); 14. Vlachava V et al, PNAS UDA 120, e2309077120 (2023); 15. Wilkinson GW et al, Med Microbiol Immunol 204, 273 (2015); 16. Patel M et al, Front Immunol 9, 2214 (2018) 17. Wang EC et al, Mol Cell Biol 21, 3451 (2001); 18. Bull MJ et al, J Exp Med 205, 2457 (2008); 19. Taraban VY et al, Mucosal Immunol 4, 186 (2011); 20. Twohig JP et al, FASEB J 26, 3675 (2012); 21. Meylan F et al, Imiwnedd 29, 79 (2008); 22. Malhotra N et al, Sci Immunol 3, eaa02892 (2018); 23. Collins FL et al, BMC Musculoskel Dis 20, 326 (2019).
Addysgu
Rwy'n darparu darlithoedd, tiwtorialau a phrosiectau ymchwil mewn Imiwnoleg a Firoleg i ystod o gyrsiau a ddarperir gan Brifysgol Caerdydd. Ar hyn o bryd, mae'r rhan fwyaf o'r rhain ar gyfer myfyrwyr meddygol (2il a 3edd flwyddyn), ond hefyd i'r cwrs Ffarmacoleg Feddygol. Rwyf hefyd yn addysgu ar yr MSc newydd mewn Imiwnoleg Glinigol Gymhwysol ac Arbrofol.
Bywgraffiad
PROFIAD GWAITH
Ebr 2009 ymlaen Is-adran Heintiau ac Imiwnedd, Ysgol Meddygaeth, Prifysgol Caerdydd
Athro: Awst 2021 ymlaen
Darllenydd: Awst 2009 i Gorff 2021
Uwch Ddarlithydd: Ebrill 2009 i Jul 2009
Awst 2005 – Ebr 2009 Ysgol Meddygaeth, Prifysgol Caerdydd
Uwch Ddarlithydd
Ionawr 2000 – Jul 2005 Adran Heintiau ac Imiwnedd, UWCM, Caerdydd
Uwch Ddarlithydd: Awst 2004 i Gorff 2005
Darlithydd: Ionawr 2000 hyd Gorffennaf 2004
Hydref 1995 – Rhagfyr 1999 Cronfa Ymchwil Canser Imperial, Llundain
Cymrawd Ymchwil Goffa Beit
Medi 1993 - Medi 1995 Adran Meddygaeth, UWCM, Caerdydd
RA Ôl-Ddoethurol
Hydref 1992 - Ionawr 1993 Adran Biocemeg Feddygol, UWCM, Caerdydd
Cyn PhD RA
Hydref 1989 - Awst 1993 Adran Meddygaeth, Prifysgol Caergrawnt (PhD)
PhD mewn Imiwnoleg
Anrhydeddau a dyfarniadau
2008 Gwobr Poster Diwydiant MRC, Brechlynnau IIB ac Arddangosfa Imiwnotherapi
2003 Grant Sefydliad Gyrfa MRC
1995 Cymrodoriaeth Goffa Beit ar gyfer Ymchwil Meddygol
1992 Gwobr Cyflwyniad Llafar, 7fed Cyfarfod Ymchwil Ôl-raddedig Blynyddol, UWCM, Caerdydd
1989 Gwobr Goffa Dr. G. Fulton Roberts am Imiwnoleg, Caergrawnt
Contact Details
Themâu ymchwil
Arbenigeddau
- Imiwnoleg cellog
- Firoleg