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Trosolwyg
Trosolwg Ymchwil
Mae fy ymchwil yn canolbwyntio ar gymhwyso technegau optegol electronau, yn enwedig microsgopeg trosglwyddo a sganio electronau, i ymchwilio i uwchstrwythur meinweoedd cysylltiol. Y rhagdybiaeth gyffredinol ar gyfer fy ngwaith fu bod cyfansoddiad a strwythur matricsau meinwe yn diffinio swyddogaeth meinwe. Yn y gorffennol rwyf wedi gweithio ym maes bioleg cyhyrysgerbydol, gan ymchwilio i strwythur cain gewynnau a chartilag, i geisio deall y newidiadau o fewn y meinweoedd hyn mewn clefydau diraddiol fel arthritis osteo- a gwynegol. Dros y deng mlynedd diwethaf rwyf wedi gweithio ar y cyd â'r Athro Andrew Quantock gan ganolbwyntio ar feinweoedd ocwlar, gan gynnwys cornbilen, sgler a gwaith rhwyll trabecwlar. Ymchwiliwyd i ystod o feinweoedd a gafwyd o lygaid dynol, trwy gydweithio lleol a rhyngwladol, i nodweddu natur rhyngweithio rhwng colagen a phroteoglycanau mewn iechyd a chlefydau.
Mae ein hastudiaethau wedi cynnwys nifer o dechnolegau blaengar ar gyfer microsgopeg, mewn perthynas â thechnegau paratoi newydd ar gyfer paratoi meinweoedd, er enghraifft defnyddio cadwraeth feinwe tymheredd isel (rhewi pwysedd uchel a rhewi amnewid), ac offeryniaeth newydd ar gyfer caffael delweddau (wyneb bloc cyfresol 3View ® SEM). Trwy gydol fy ngyrfa rwyf wedi cadw diddordeb brwd mewn methodoleg newydd ar gyfer microsgopeg electronau. Rwyf wedi defnyddio dulliau lleoleiddio arbenigol gan ddefnyddio marcwyr gwrthgyrff penodol i nodi gwahaniaethau munud mewn cydrannau meinwe, proteoglycans, yn ystod datblygiad y gornbilen yn yr embryo. Mae'n ymddangos bod y rhain yn bwysig ar gyfer aeddfedu matrics tryloyw, ei hun yn hanfodol ar gyfer gweledigaeth. Mae'r un moleciwlau meinwe hefyd yn ymwneud â phathogenau cyflyrau dallu penodol lle mae diffygion ensym yn arwain at opacities yn y gornbilen, gan ofyn am drawsblaniad cornbilen ar gyfer triniaeth.
Ar hyn o bryd mae diddordeb enfawr mewn technegau delweddu 3D, nid yn unig mewn delweddu diagnostig fel gydag OCT, ond hefyd ar lefel celloedd sengl a macromoleciwlau matrics. Mae'r dulliau hyn o tomograffeg electronau ac, yn fwyaf diweddar, microsgopeg electron sganio wyneb bloc cyfresol yn cael eu defnyddio yn ein labordy microsgopeg electronau yn y Grŵp Ymchwil Bioffiseg Strwythurol.
Trosolwg Addysgu
Ar hyn o bryd rwy'n cynorthwyo gydag addysgu mewn labordy myfyrwyr ymchwil ôl-raddedig ac israddedigion sy'n cyflawni prosiectau ymchwil blwyddyn olaf.
Cyhoeddiadau Dethol
Ifanc, RD, Knupp, C, Pinali, C, Png, K MY, Ralphs, J R, Bushby, AJ, Starborg, T, Kadler, KE, Quantock AJ. 2014. Agweddau tri dimensiwn ar gynulliad matrics gan gelloedd yn nhrafodion y gornbilen sy'n datblygu Academi Genedlaethol y Gwyddorau 111 (2) 687-692
Ifanc, RD, Liskova, P, Pinali, C, Palka, BP, Palos, M, Jirsova, K, Hrdlickova, E, Tesarova, M, Elleder, M, Zeman, J, Meek, KM, Knupp, C a Quantock, AJ. 2011. Cymhlethdodau Proteoglycan Mawr a Phensaernïaeth Colagen Aflonyddedig yn Matrics Allgellog Corneal o Mucopolysaccharidosis Math VII (Syndrom Sly). Offthalmoleg Ymchwiliol a Gwyddoniaeth Weledol 52 (9) 6720-6728.
Ifanc, RD, Swamynathan, SK, Boote, C, Mann, M, Quantock, AJ, Piatigorsky, J, Funderburgh, JL, Meek, KM. 2009. Mae Edema Stromal yn Klf4 Conditional Null Mouse Cornea yn gysylltiedig â threfniadaeth Fibril Colagen wedi'i newid a phroteoglycansau llai. Offthalmoleg Ymchwiliol a Gwyddoniaeth Weledol 50 (9) 4155-4161
Ifanc, RD, Akama, TO, Liskova, P, Ebenezer, ND, Allan, B, Kerr, B, Caterson, B, Fukuda, MN, Quantock, AJ. 2007. Lleoleiddio immunogold gwahaniaethol o proteoglycans sylffad wedi'u sulpheiddio a heb eu hinswleiddio mewn gornbilen dystrophy arferol a macwlaidd gan ddefnyddio gwrthgyrff sy'n benodol i motiffau sylffu. Histochemistry a Bioleg Cell 127 (1), 115-120
Ifanc, RD, Quantock, AJ, Sotozono, C, Koizumi, N, Kinoshita, S. 2006. Patrymau sylffad keratan proteoglycan mewn sclerocornea yn debyg i gornbilen yn hytrach na sgleria. British Journal of Offthalmology 90 (3), 391-393
Cyhoeddiad
2024
- Regini, J. W. et al. 2024. Membrane structures and functional correlates in the bi-segmented eye lens of the cephalopod. Biology Open 13(9), article number: bio060445. (10.1242/bio.060445)
2023
- Bains, K. K., Young, R. D., Koudouna, E., Lewis, P. N. and Quantock, A. J. 2023. Cell–cell and cell–matrix interactions at the presumptive stem cell niche of the chick corneal limbus. Cells 12(19), article number: 2334. (10.3390/cells12192334)
- Koudouna, E., Young, R. D., Quantock, A. J. and Ralphs, J. R. 2023. Developmental changes in patterns of distribution of fibronectin and tenascin-C in the chicken cornea: evidence for distinct and independent functions during corneal development and morphogenesis. International Journal of Molecular Sciences 24(4), article number: 3555. (10.3390/ijms24043555)
- Bains, K. K., Ashworth, S., Koudouna, E., Young, R. D., Hughes, C. E. and Quantock, A. J. 2023. Chondroitin sulphate/dermatan sulphate proteoglycans: potential regulators of corneal stem/progenitor cell phenotype in vitro. International Journal of Molecular Sciences 24(3), article number: 2095. (10.3390/ijms24032095)
2022
- Hayashi, R. et al. 2022. Generation of 3D lacrimal gland organoids from human pluripotent stem cells. Nature 605(7908), pp. 126-131. (10.1038/s41586-022-04613-4)
2021
- Koudouna, E., Mikula, E., Brown, D. J., Young, R. D., Quantock, A. J. and Jester, J. V. 2021. Response to letter to Editor “comments on ‘cell regulation of collagen fibril macrostructure during corneal morphogenesis’ by Koudouna et al.”. Acta Biomaterialia 136, pp. 594-595. (10.1016/j.actbio.2021.09.061)
- Lewis, P. N., Young, R. D., Souza, R. B., Quantock, A. J. and Meek, K. M. 2021. Contrast-enhanced tissue processing of fibrillin-rich elastic fibres for 3D visualization by volume scanning electron microscopy. Methods and Protocols 4(3), article number: 56. (10.3390/mps4030056)
- Ashworth, S. et al. 2021. Chondroitin sulfate as a potential modulator of the stem cell niche in cornea. Frontiers in Cell and Developmental Biology 8, article number: 567358. (10.3389/fcell.2020.567358)
2020
- Young, R. D. et al. 2020. Observations on nascent matrix structures in embryonic cornea: Important in cell interactions, or merely vestiges of the lens surface?. Archives of Clinical and Experimental Ophthalmology 2(2), pp. 67-72. (10.46439/ophthalmology.2.014)
- Hammond, G. M., Young, R. D., Muir, D. D. and Quantock, A. J. 2020. The microanatomy of Bowman’s layer in the cornea of the pig: changes in collagen fibril architecture at the corneoscleral limbus. European Journal of Anatomy 24(5), pp. 399-406.
- Wang, K. et al. 2020. Cell compaction is not required for the development of gradient refractive index profiles in the embryonic chick lens. Experimental Eye Research 197, article number: 108112. (10.1016/j.exer.2020.108112)
2019
- Young, R. D. et al. 2019. Cell-independent matrix configuration in early corneal development. Experimental Eye Research 187, article number: 107772. (10.1016/j.exer.2019.107772)
- Lewis, P., White, T., Feneck, E., Young, R. and Meek, K. 2019. Elastin content and distribution in endothelial keratoplasty tissue determines direction of scrolling. American Journal of Ophthalmology 197, pp. 181-182. (10.1016/j.ajo.2018.08.047)
2018
- Koudouna, E., Mikula, E., Brown, D. J., Young, R. D., Quantock, A. J. and Jester, J. V. 2018. Cell regulation of collagen fibril macrostructure during corneal morphogenesis. Acta Biomaterialia 79, pp. 96-112. (10.1016/j.actbio.2018.08.017)
- Littlechild, S. et al. 2018. Keratan sulfate phenotype in the β-1,3-N-acetylglucosaminyltransferase-7-null mouse cornea. Investigative Ophthalmology and Visual Science 59, pp. 1641-1651. (10.1167/iovs.17-22716)
2017
- Okumura, N. et al. 2017. Sustained activation of the unfolded protein response induces cell death in Fuchs' endothelial corneal dystrophy. Investigative Ophthalmology & Visual Science 58(9), pp. 3697-3707. (10.1167/iovs.16-21023)
- White, T., Lewis, P., Young, R. D., Kitazawa, K., Inatomi, T., Kinoshita, S. and Meek, K. M. A. 2017. Elastic microfibril distribution in the cornea: Differences between normal and keratoconic stroma. Experimental Eye Research 159, pp. 40-48. (10.1016/j.exer.2017.03.002)
- Akhbanbetova, A. et al. 2017. A surgical cryoprobe for targeted transcorneal freezing and endothelial cell removal. Journal of Ophthalmology 2017, article number: 5614089. (10.1155/2017/5614089)
2016
- Parfitt, G., Lewis, P. N., Young, R. D., Richardson, A., Lyons, J., Di Girolamo, N. and Jester, J. 2016. Renewal of the holocrine meibomian glands by label-retaining, unipotent epithelial progenitors. Stem Cell Reports 7(3), pp. 399-410. (10.1016/j.stemcr.2016.07.010)
- Lewis, P., White, T., Young, R. D., Bell, J. S., Winlove, C. P. and Meek, K. M. A. 2016. Three-dimensional arrangement of elastic fibers in the human corneal stroma. Experimental Eye Research 146, pp. 43-53. (10.1016/j.exer.2015.12.006)
- Kamma-Lorger, C. S. et al. 2016. Role of Decorin core protein in collagen organisation in Congenital Stromal Corneal Dystrophy (CSCD). PLoS ONE 11(2), article number: e0147948. (10.1371/journal.pone.0147948)
2015
- Yamada, K. et al. 2015. Mesenchymal-epithelial cell interactions and proteoglycan matrix composition in the presumptive stem cell niche of the rabbit corneal limbus. Molecular Vision 21, pp. 1328-1329.
- Okumura, N. et al. 2015. Involvement of ZEB1 and Snail1 in excessive production of extracellular matrix in Fuchs endothelial corneal dystrophy. Laboratory Investigation 95, pp. 1291-1304. (10.1038/labinvest.2015.111)
- Quantock, A. J., Winkler, M., Parfitt, G. J., Young, R. D., Brown, D. J., Boote, C. and Jester, J. V. 2015. From nano to macro: Studying the hierarchical structure of the corneal extracellular matrix. Experimental Eye Research 133, pp. 81-99. (10.1016/j.exer.2014.07.018)
2014
- White, T., Lewis, P., Young, R., Meek, K. and Bell, J. 2014. 3D structural studies of the cornea. Presented at: British Congress of Optometry and Vision Science, Cardiff, 01 November 2014. , (10.1111/opo.12160)
- Ho, L. T. Y. et al. 2014. A comparison of glycosaminoglycan distributions, keratan sulphate sulphation patterns and collagen fibril architecture from central to peripheral regions of the bovine cornea. Matrix Biology 38, pp. 59-68. (10.1016/j.matbio.2014.06.004)
- Young, R. D. et al. 2014. Three-dimensional aspects of matrix assembly by cells in the developing cornea. Proceedings of the National Academy of Sciences 111(2), pp. 687-692. (10.1073/pnas.1313561110)
2013
- Khan, I. M. et al. 2013. In vitro growth factor-induced bio engineering of mature articular cartilage. Biomaterials 34(5), pp. 1478-1487. (10.1016/j.biomaterials.2012.09.076)
- Hayes, S. et al. 2013. The effect of Riboflavin/UVA collagen cross-linking therapy on the structure and hydrodynamic behaviour of the ungulate and rabbit corneal stroma. PLoS ONE 8(1), article number: e52860. (10.1371/journal.pone.0052860)
2011
- Koudouna, E. et al. 2011. Preliminary electron microscopical studies of connective tissue in the human lamina cribrosa [Abstract]. International Journal of Experimental Pathology 92(6), pp. A26. (10.1111/j.1365-2613.2011.00780.x)
- Khan, I. M., Evans, S. L., Young, R. D., Blain, E. J., Quantock, A. J., Avery, N. and Archer, C. W. 2011. Fibroblast growth factor 2 and transforming growth factor β1 induce precocious maturation of articular cartilage. Arthritis & Rheumatism 63(11), pp. 3417-3427. (10.1002/art.30543)
- Bushby, A. J., P'ng, K. M. Y., Young, R. D., Pinali, C., Knupp, C. and Quantock, A. J. 2011. Imaging three-dimensional tissue architectures by focused ion beam scanning electron microscopy. Nature Protocols 6(6), pp. 845-858. (10.1038/nprot.2011.332)
- Parfitt, G., Pinali, C., Akama, T. O., Young, R. D., Nishida, K., Quantock, A. J. and Knupp, C. 2011. Electron tomography reveals multiple self-association of chondroitin sulphate/dermatan sulphate proteoglycans in Chst5-null mouse corneas. Journal of Structural Biology 174(3), pp. 536-541. (10.1016/j.jsb.2011.03.015)
- Jones, F. E., Hatanaka, H., Young, R. D., Koizumi, N., Kinoshita, S., Okumura, N. and Quantock, A. J. 2011. Investigation into endothelial cell morphology following new methods of posterior corneal surgery [Abstract]. International Journal of Experimental Pathology 92(3), pp. A24. (10.1111/j.1365-2613.2010.00759.x)
- Young, R. D. et al. 2011. Large Proteoglycan Complexes and Disturbed Collagen Architecture in the Corneal Extracellular Matrix of Mucopolysaccharidosis Type VII (Sly Syndrome). Investigative Ophthalmology & Visual Science 52(9), pp. 6720-6728. (10.1167/iovs.11-7377)
- Hayes, S. et al. 2011. The effect of vitamin C deficiency and chronic ultraviolet-B exposure on corneal ultrastructure: a preliminary investigation. Molecular Vision 17, pp. 3107-3115.
2010
- Parfitt, G. J., Pinali, C., Young, R. D., Quantock, A. J. and Knupp, C. 2010. Three-dimensional reconstruction of collagen-proteoglycan interactions in the mouse corneal stroma by electron tomography. Journal of Structural Biology 170(2), pp. 392-397. (10.1016/j.jsb.2010.01.019)
- Liles, M. et al. 2010. Differential relative sulfation of keratan sulfate glycosaminoglycan in the chick cornea during embryonic development. Investigative Ophthalmology and Visual Science 51(3), pp. 1365-1372. (10.1167/iovs.09-4004)
- Lewis, P., Pinali, C., Young, R. D., Meek, K. M. A., Quantock, A. J. and Knupp, C. 2010. Structural interactions between collagen and proteoglycans are elucidated by three-dimensional electron tomography of bovine cornea. Structure 18(2), pp. 239-245. (10.1016/j.str.2009.11.013)
- Quantock, A. J., Young, R. D. and Akama, T. O. 2010. Structural and biochemical aspects of keratan sulphate in the cornea. Cellular and Molecular Life Sciences 67(6), pp. 891-906. (10.1007/s00018-009-0228-7)
- Palka, B. P. et al. 2010. Structural Collagen Alterations in Macular Corneal Dystrophy Occur Mainly in the Posterior Stroma. Current Eye Research 35(7), pp. 580-586. (10.3109/02713681003760150)
- Kamma-Lorger, C. S. et al. 2010. Collagen and mature elastic fibre organisation as a function of depth in the human cornea and limbus. Journal of Structural Biology 169(3), pp. 424-430. (10.1016/j.jsb.2009.11.004)
2009
- Boote, C. et al. 2009. Ultrastructural changes in the retinopathy, globe enlarged (rge) chick cornea. Journal of Structural Biology 166(2), pp. 195-204. (10.1016/j.jsb.2009.01.009)
- Palka, B. P., Young, R. D., Meek, K. M. A., Tanioka, H., Kinoshita, S., Yagi, N. and Quantock, A. J. 2009. Depth-profiled synchrotron microbeam analysis of collagen fibril ultrastructure in the developing chick cornea [Abstract]. International Journal of Experimental Pathology 90(2), pp. A126. (10.1111/j.1365-2613.2009.00644.x)
- Gealy, E. C., Hayes, A. J., Buckwell, R., Young, R. D., Caterson, B., Quantock, A. J. and Ralphs, J. R. 2009. Actin and type I collagen propeptide distribution in the developing chick cornea. Investigative Ophthalmology & Visual Science 50(4), pp. 1653 -1658. (10.1167/iovs.08-2554)
- Young, R. D. et al. 2009. Stromal Edema in Klf4 Conditional Null Mouse Cornea Is Associated with Altered Collagen Fibril Organization and Reduced Proteoglycans. Investigative Ophthalmology & Visual Science 50(9), pp. 4155-4161. (10.1167/iovs.09-3561)
- Knupp, C., Pinali, C., Lewis, P., Parfitt, G. J., Young, R. D., Meek, K. M. A. and Quantock, A. J. 2009. The architecture of the cornea and structural basis of its transparency. In: McPherson, A. ed. Advances in Protein Chemistry and Structural Biology., Vol. 78. London: Academic Press, pp. 25-49., (10.1016/S1876-1623(08)78002-7)
- Hayes, S., Young, R. D., Boote, C., Hawksworth, N., Huang, Y. and Meek, K. M. A. 2009. A structural investigation of corneal graft failure in suspected recurrent keratoconus. Eye 24(4), pp. 728-734. (10.1038/eye.2009.159)
2008
- Yamasaki, K. et al. 2008. Genomic Aberrations and Cellular Heterogeneity in SV40-Immortalized Human Corneal Epithelial Cells. Investigative Ophthalmology & Visual Science 50(2), pp. 604-613. (10.1167/iovs.08-2239)
- Quantock, A. J. and Young, R. D. 2008. Development of the corneal stroma, and the collagen-proteoglycan associations that help define its structure and function. Developmental Dynamics 237(10), pp. 2607-2621. (10.1002/dvdy.21579)
2007
- Young, R. D., Gealy, E. C., Liles, M., Caterson, B., Ralphs, J. R. and Quantock, A. J. 2007. Keratan sulfate glycosaminoglycan and the association with collagen fibrils in rudimentary lamellae in the developing avian cornea. Investigative Ophthalmology and Visual Science 48(7), pp. 3083-3088. (10.1167/iovs.06-1323)
- Albon, J. et al. 2007. Connective tissue structure of the tree shrew optic nerve and associated ageing changes. Investigative Ophthalmology and Visual Science 48(5), pp. 2134-2144. (10.1167/iovs.06-0084)
- Young, R. D. et al. 2007. Differential immunogold localisation of sulphated and unsulphated keratan sulphate proteoglycans in normal and macular dystrophy cornea using sulphation motif-specific antibodies. Histochemistry and Cell Biology 127(1), pp. 115-120. (10.1007/s00418-006-0228-8)
- Gealy, E. C. et al. 2007. Differential expression of the keratan sulphate proteoglycan, keratocan, during chick corneal embryogenesis. Histochemistry and Cell Biology 128(6), pp. 551-555. (10.1007/s00418-007-0332-4)
- Quantock, A. J. et al. 2007. Small-angle fibre diffraction studies of corneal matrix structure: a depth profiled investigation of the human eye-bank cornea. Journal of Applied Crystallography 40(S1), pp. S335-S340. (10.1107/S0021889807005523)
2006
- Hayashida, Y. et al. 2006. Matrix morphogenesis in cornea is mediated by the modification of keratan sulfate by GlcNAc 6-O-sulfotransferase. PNAS 103(36), pp. 13333-13338. (10.1073/pnas.0605441103)
- Young, R. D., Quantock, A. J., Sotozono, C., Koizumi, N. and Kinoshita, S. 2006. Sulphation patterns of keratan sulphate proteoglycan in sclerocornea resemble cornea rather than sclera [Letter]. British Journal of Ophthalmology 90(3), pp. 391-393. (10.1136/bjo.2005.085803)
- Young, R. D. et al. 2006. Immunogold localization of keratan sulphate in macular corneal dystrophy using monoclonal antibodies to specific sulphation motifs [Abstract]. International Journal of Experimental Pathology 87(1), pp. A49-A50. (10.1111/j.0959-9673.2006.00458.x)
2005
- Quantock, A. J., Sano, Y., Young, R. D. and Kinoshita, S. 2005. Stromal architecture and immune tolerance in additive corneal xenografts in rodents. Acta Ophthalmologica Scandinavica 83(4), pp. 462-466. (10.1111/j.1600-0420.2005.00509.x)
- Young, R. D. et al. 2005. Atypical composition and ultrastructure of proteoglycans in the mouse corneal stroma. Investigative Ophthalmology and Visual Science 46(6), pp. 1973-1978. (10.1167/iovs.04-1309)
2003
- Connon, C. J., Young, R. D. and Kidd, E. J. 2003. P2X7 receptors are redistributed on human monocytes after pore formation in response to prolonged agonist exposure. Pharmacology 67(3), pp. 163-168. (10.1159/000067795)
2000
- Young, R. D., Lawrence, P. A., Duance, V. C., Aigner, T. and Monaghan, P. 2000. Immunolocalization of collagen Types II and III in single fibrils of human articular cartilage. Journal of Histochemistry and Cytochemistry 48(3), pp. 423-432. (10.1177/002215540004800312)
Articles
- Regini, J. W. et al. 2024. Membrane structures and functional correlates in the bi-segmented eye lens of the cephalopod. Biology Open 13(9), article number: bio060445. (10.1242/bio.060445)
- Bains, K. K., Young, R. D., Koudouna, E., Lewis, P. N. and Quantock, A. J. 2023. Cell–cell and cell–matrix interactions at the presumptive stem cell niche of the chick corneal limbus. Cells 12(19), article number: 2334. (10.3390/cells12192334)
- Koudouna, E., Young, R. D., Quantock, A. J. and Ralphs, J. R. 2023. Developmental changes in patterns of distribution of fibronectin and tenascin-C in the chicken cornea: evidence for distinct and independent functions during corneal development and morphogenesis. International Journal of Molecular Sciences 24(4), article number: 3555. (10.3390/ijms24043555)
- Bains, K. K., Ashworth, S., Koudouna, E., Young, R. D., Hughes, C. E. and Quantock, A. J. 2023. Chondroitin sulphate/dermatan sulphate proteoglycans: potential regulators of corneal stem/progenitor cell phenotype in vitro. International Journal of Molecular Sciences 24(3), article number: 2095. (10.3390/ijms24032095)
- Hayashi, R. et al. 2022. Generation of 3D lacrimal gland organoids from human pluripotent stem cells. Nature 605(7908), pp. 126-131. (10.1038/s41586-022-04613-4)
- Koudouna, E., Mikula, E., Brown, D. J., Young, R. D., Quantock, A. J. and Jester, J. V. 2021. Response to letter to Editor “comments on ‘cell regulation of collagen fibril macrostructure during corneal morphogenesis’ by Koudouna et al.”. Acta Biomaterialia 136, pp. 594-595. (10.1016/j.actbio.2021.09.061)
- Lewis, P. N., Young, R. D., Souza, R. B., Quantock, A. J. and Meek, K. M. 2021. Contrast-enhanced tissue processing of fibrillin-rich elastic fibres for 3D visualization by volume scanning electron microscopy. Methods and Protocols 4(3), article number: 56. (10.3390/mps4030056)
- Ashworth, S. et al. 2021. Chondroitin sulfate as a potential modulator of the stem cell niche in cornea. Frontiers in Cell and Developmental Biology 8, article number: 567358. (10.3389/fcell.2020.567358)
- Young, R. D. et al. 2020. Observations on nascent matrix structures in embryonic cornea: Important in cell interactions, or merely vestiges of the lens surface?. Archives of Clinical and Experimental Ophthalmology 2(2), pp. 67-72. (10.46439/ophthalmology.2.014)
- Hammond, G. M., Young, R. D., Muir, D. D. and Quantock, A. J. 2020. The microanatomy of Bowman’s layer in the cornea of the pig: changes in collagen fibril architecture at the corneoscleral limbus. European Journal of Anatomy 24(5), pp. 399-406.
- Wang, K. et al. 2020. Cell compaction is not required for the development of gradient refractive index profiles in the embryonic chick lens. Experimental Eye Research 197, article number: 108112. (10.1016/j.exer.2020.108112)
- Young, R. D. et al. 2019. Cell-independent matrix configuration in early corneal development. Experimental Eye Research 187, article number: 107772. (10.1016/j.exer.2019.107772)
- Lewis, P., White, T., Feneck, E., Young, R. and Meek, K. 2019. Elastin content and distribution in endothelial keratoplasty tissue determines direction of scrolling. American Journal of Ophthalmology 197, pp. 181-182. (10.1016/j.ajo.2018.08.047)
- Koudouna, E., Mikula, E., Brown, D. J., Young, R. D., Quantock, A. J. and Jester, J. V. 2018. Cell regulation of collagen fibril macrostructure during corneal morphogenesis. Acta Biomaterialia 79, pp. 96-112. (10.1016/j.actbio.2018.08.017)
- Littlechild, S. et al. 2018. Keratan sulfate phenotype in the β-1,3-N-acetylglucosaminyltransferase-7-null mouse cornea. Investigative Ophthalmology and Visual Science 59, pp. 1641-1651. (10.1167/iovs.17-22716)
- Okumura, N. et al. 2017. Sustained activation of the unfolded protein response induces cell death in Fuchs' endothelial corneal dystrophy. Investigative Ophthalmology & Visual Science 58(9), pp. 3697-3707. (10.1167/iovs.16-21023)
- White, T., Lewis, P., Young, R. D., Kitazawa, K., Inatomi, T., Kinoshita, S. and Meek, K. M. A. 2017. Elastic microfibril distribution in the cornea: Differences between normal and keratoconic stroma. Experimental Eye Research 159, pp. 40-48. (10.1016/j.exer.2017.03.002)
- Akhbanbetova, A. et al. 2017. A surgical cryoprobe for targeted transcorneal freezing and endothelial cell removal. Journal of Ophthalmology 2017, article number: 5614089. (10.1155/2017/5614089)
- Parfitt, G., Lewis, P. N., Young, R. D., Richardson, A., Lyons, J., Di Girolamo, N. and Jester, J. 2016. Renewal of the holocrine meibomian glands by label-retaining, unipotent epithelial progenitors. Stem Cell Reports 7(3), pp. 399-410. (10.1016/j.stemcr.2016.07.010)
- Lewis, P., White, T., Young, R. D., Bell, J. S., Winlove, C. P. and Meek, K. M. A. 2016. Three-dimensional arrangement of elastic fibers in the human corneal stroma. Experimental Eye Research 146, pp. 43-53. (10.1016/j.exer.2015.12.006)
- Kamma-Lorger, C. S. et al. 2016. Role of Decorin core protein in collagen organisation in Congenital Stromal Corneal Dystrophy (CSCD). PLoS ONE 11(2), article number: e0147948. (10.1371/journal.pone.0147948)
- Yamada, K. et al. 2015. Mesenchymal-epithelial cell interactions and proteoglycan matrix composition in the presumptive stem cell niche of the rabbit corneal limbus. Molecular Vision 21, pp. 1328-1329.
- Okumura, N. et al. 2015. Involvement of ZEB1 and Snail1 in excessive production of extracellular matrix in Fuchs endothelial corneal dystrophy. Laboratory Investigation 95, pp. 1291-1304. (10.1038/labinvest.2015.111)
- Quantock, A. J., Winkler, M., Parfitt, G. J., Young, R. D., Brown, D. J., Boote, C. and Jester, J. V. 2015. From nano to macro: Studying the hierarchical structure of the corneal extracellular matrix. Experimental Eye Research 133, pp. 81-99. (10.1016/j.exer.2014.07.018)
- Ho, L. T. Y. et al. 2014. A comparison of glycosaminoglycan distributions, keratan sulphate sulphation patterns and collagen fibril architecture from central to peripheral regions of the bovine cornea. Matrix Biology 38, pp. 59-68. (10.1016/j.matbio.2014.06.004)
- Young, R. D. et al. 2014. Three-dimensional aspects of matrix assembly by cells in the developing cornea. Proceedings of the National Academy of Sciences 111(2), pp. 687-692. (10.1073/pnas.1313561110)
- Khan, I. M. et al. 2013. In vitro growth factor-induced bio engineering of mature articular cartilage. Biomaterials 34(5), pp. 1478-1487. (10.1016/j.biomaterials.2012.09.076)
- Hayes, S. et al. 2013. The effect of Riboflavin/UVA collagen cross-linking therapy on the structure and hydrodynamic behaviour of the ungulate and rabbit corneal stroma. PLoS ONE 8(1), article number: e52860. (10.1371/journal.pone.0052860)
- Koudouna, E. et al. 2011. Preliminary electron microscopical studies of connective tissue in the human lamina cribrosa [Abstract]. International Journal of Experimental Pathology 92(6), pp. A26. (10.1111/j.1365-2613.2011.00780.x)
- Khan, I. M., Evans, S. L., Young, R. D., Blain, E. J., Quantock, A. J., Avery, N. and Archer, C. W. 2011. Fibroblast growth factor 2 and transforming growth factor β1 induce precocious maturation of articular cartilage. Arthritis & Rheumatism 63(11), pp. 3417-3427. (10.1002/art.30543)
- Bushby, A. J., P'ng, K. M. Y., Young, R. D., Pinali, C., Knupp, C. and Quantock, A. J. 2011. Imaging three-dimensional tissue architectures by focused ion beam scanning electron microscopy. Nature Protocols 6(6), pp. 845-858. (10.1038/nprot.2011.332)
- Parfitt, G., Pinali, C., Akama, T. O., Young, R. D., Nishida, K., Quantock, A. J. and Knupp, C. 2011. Electron tomography reveals multiple self-association of chondroitin sulphate/dermatan sulphate proteoglycans in Chst5-null mouse corneas. Journal of Structural Biology 174(3), pp. 536-541. (10.1016/j.jsb.2011.03.015)
- Jones, F. E., Hatanaka, H., Young, R. D., Koizumi, N., Kinoshita, S., Okumura, N. and Quantock, A. J. 2011. Investigation into endothelial cell morphology following new methods of posterior corneal surgery [Abstract]. International Journal of Experimental Pathology 92(3), pp. A24. (10.1111/j.1365-2613.2010.00759.x)
- Young, R. D. et al. 2011. Large Proteoglycan Complexes and Disturbed Collagen Architecture in the Corneal Extracellular Matrix of Mucopolysaccharidosis Type VII (Sly Syndrome). Investigative Ophthalmology & Visual Science 52(9), pp. 6720-6728. (10.1167/iovs.11-7377)
- Hayes, S. et al. 2011. The effect of vitamin C deficiency and chronic ultraviolet-B exposure on corneal ultrastructure: a preliminary investigation. Molecular Vision 17, pp. 3107-3115.
- Parfitt, G. J., Pinali, C., Young, R. D., Quantock, A. J. and Knupp, C. 2010. Three-dimensional reconstruction of collagen-proteoglycan interactions in the mouse corneal stroma by electron tomography. Journal of Structural Biology 170(2), pp. 392-397. (10.1016/j.jsb.2010.01.019)
- Liles, M. et al. 2010. Differential relative sulfation of keratan sulfate glycosaminoglycan in the chick cornea during embryonic development. Investigative Ophthalmology and Visual Science 51(3), pp. 1365-1372. (10.1167/iovs.09-4004)
- Lewis, P., Pinali, C., Young, R. D., Meek, K. M. A., Quantock, A. J. and Knupp, C. 2010. Structural interactions between collagen and proteoglycans are elucidated by three-dimensional electron tomography of bovine cornea. Structure 18(2), pp. 239-245. (10.1016/j.str.2009.11.013)
- Quantock, A. J., Young, R. D. and Akama, T. O. 2010. Structural and biochemical aspects of keratan sulphate in the cornea. Cellular and Molecular Life Sciences 67(6), pp. 891-906. (10.1007/s00018-009-0228-7)
- Palka, B. P. et al. 2010. Structural Collagen Alterations in Macular Corneal Dystrophy Occur Mainly in the Posterior Stroma. Current Eye Research 35(7), pp. 580-586. (10.3109/02713681003760150)
- Kamma-Lorger, C. S. et al. 2010. Collagen and mature elastic fibre organisation as a function of depth in the human cornea and limbus. Journal of Structural Biology 169(3), pp. 424-430. (10.1016/j.jsb.2009.11.004)
- Boote, C. et al. 2009. Ultrastructural changes in the retinopathy, globe enlarged (rge) chick cornea. Journal of Structural Biology 166(2), pp. 195-204. (10.1016/j.jsb.2009.01.009)
- Palka, B. P., Young, R. D., Meek, K. M. A., Tanioka, H., Kinoshita, S., Yagi, N. and Quantock, A. J. 2009. Depth-profiled synchrotron microbeam analysis of collagen fibril ultrastructure in the developing chick cornea [Abstract]. International Journal of Experimental Pathology 90(2), pp. A126. (10.1111/j.1365-2613.2009.00644.x)
- Gealy, E. C., Hayes, A. J., Buckwell, R., Young, R. D., Caterson, B., Quantock, A. J. and Ralphs, J. R. 2009. Actin and type I collagen propeptide distribution in the developing chick cornea. Investigative Ophthalmology & Visual Science 50(4), pp. 1653 -1658. (10.1167/iovs.08-2554)
- Young, R. D. et al. 2009. Stromal Edema in Klf4 Conditional Null Mouse Cornea Is Associated with Altered Collagen Fibril Organization and Reduced Proteoglycans. Investigative Ophthalmology & Visual Science 50(9), pp. 4155-4161. (10.1167/iovs.09-3561)
- Hayes, S., Young, R. D., Boote, C., Hawksworth, N., Huang, Y. and Meek, K. M. A. 2009. A structural investigation of corneal graft failure in suspected recurrent keratoconus. Eye 24(4), pp. 728-734. (10.1038/eye.2009.159)
- Yamasaki, K. et al. 2008. Genomic Aberrations and Cellular Heterogeneity in SV40-Immortalized Human Corneal Epithelial Cells. Investigative Ophthalmology & Visual Science 50(2), pp. 604-613. (10.1167/iovs.08-2239)
- Quantock, A. J. and Young, R. D. 2008. Development of the corneal stroma, and the collagen-proteoglycan associations that help define its structure and function. Developmental Dynamics 237(10), pp. 2607-2621. (10.1002/dvdy.21579)
- Young, R. D., Gealy, E. C., Liles, M., Caterson, B., Ralphs, J. R. and Quantock, A. J. 2007. Keratan sulfate glycosaminoglycan and the association with collagen fibrils in rudimentary lamellae in the developing avian cornea. Investigative Ophthalmology and Visual Science 48(7), pp. 3083-3088. (10.1167/iovs.06-1323)
- Albon, J. et al. 2007. Connective tissue structure of the tree shrew optic nerve and associated ageing changes. Investigative Ophthalmology and Visual Science 48(5), pp. 2134-2144. (10.1167/iovs.06-0084)
- Young, R. D. et al. 2007. Differential immunogold localisation of sulphated and unsulphated keratan sulphate proteoglycans in normal and macular dystrophy cornea using sulphation motif-specific antibodies. Histochemistry and Cell Biology 127(1), pp. 115-120. (10.1007/s00418-006-0228-8)
- Gealy, E. C. et al. 2007. Differential expression of the keratan sulphate proteoglycan, keratocan, during chick corneal embryogenesis. Histochemistry and Cell Biology 128(6), pp. 551-555. (10.1007/s00418-007-0332-4)
- Quantock, A. J. et al. 2007. Small-angle fibre diffraction studies of corneal matrix structure: a depth profiled investigation of the human eye-bank cornea. Journal of Applied Crystallography 40(S1), pp. S335-S340. (10.1107/S0021889807005523)
- Hayashida, Y. et al. 2006. Matrix morphogenesis in cornea is mediated by the modification of keratan sulfate by GlcNAc 6-O-sulfotransferase. PNAS 103(36), pp. 13333-13338. (10.1073/pnas.0605441103)
- Young, R. D., Quantock, A. J., Sotozono, C., Koizumi, N. and Kinoshita, S. 2006. Sulphation patterns of keratan sulphate proteoglycan in sclerocornea resemble cornea rather than sclera [Letter]. British Journal of Ophthalmology 90(3), pp. 391-393. (10.1136/bjo.2005.085803)
- Young, R. D. et al. 2006. Immunogold localization of keratan sulphate in macular corneal dystrophy using monoclonal antibodies to specific sulphation motifs [Abstract]. International Journal of Experimental Pathology 87(1), pp. A49-A50. (10.1111/j.0959-9673.2006.00458.x)
- Quantock, A. J., Sano, Y., Young, R. D. and Kinoshita, S. 2005. Stromal architecture and immune tolerance in additive corneal xenografts in rodents. Acta Ophthalmologica Scandinavica 83(4), pp. 462-466. (10.1111/j.1600-0420.2005.00509.x)
- Young, R. D. et al. 2005. Atypical composition and ultrastructure of proteoglycans in the mouse corneal stroma. Investigative Ophthalmology and Visual Science 46(6), pp. 1973-1978. (10.1167/iovs.04-1309)
- Connon, C. J., Young, R. D. and Kidd, E. J. 2003. P2X7 receptors are redistributed on human monocytes after pore formation in response to prolonged agonist exposure. Pharmacology 67(3), pp. 163-168. (10.1159/000067795)
- Young, R. D., Lawrence, P. A., Duance, V. C., Aigner, T. and Monaghan, P. 2000. Immunolocalization of collagen Types II and III in single fibrils of human articular cartilage. Journal of Histochemistry and Cytochemistry 48(3), pp. 423-432. (10.1177/002215540004800312)
Book sections
- Knupp, C., Pinali, C., Lewis, P., Parfitt, G. J., Young, R. D., Meek, K. M. A. and Quantock, A. J. 2009. The architecture of the cornea and structural basis of its transparency. In: McPherson, A. ed. Advances in Protein Chemistry and Structural Biology., Vol. 78. London: Academic Press, pp. 25-49., (10.1016/S1876-1623(08)78002-7)
Conferences
- White, T., Lewis, P., Young, R., Meek, K. and Bell, J. 2014. 3D structural studies of the cornea. Presented at: British Congress of Optometry and Vision Science, Cardiff, 01 November 2014. , (10.1111/opo.12160)
Ymchwil
Prosiectau Ymchwil Cyfredol
* Astudiaethau strwythurol o gornbilen ar ôl rhewi gyda phrototeip cryoprobe newydd.
* Ailadeiladu keratocytes 3D a matrics allgellog wrth ddatblygu gornbilen adar trwy ficrosgopeg electron sganio wyneb bloc cyfresol i ymchwilio i ddatblygiad strômal corneal.
* Ymchwiliad uwchstrwythurol cymharol i'r rhwyll trabecwlar yn llygaid dynol normal a glawcomatous.
* Astudiaethau microsgopig o gelloedd endothelaidd gornbilen yn dystroffi endothelaidd corneal Fuchs.
Cyllid
Quantock AJ (PI), K Meek & C Tucker: £836,976. Nodweddu ffisegol o fecanweithiau cynulliad a throsglwyddo golau yn y gornbilen. Grant prosiect EPSRC. 2008 – 2011
Cydweithredwyr Ymchwil
Yr Athro Shigeru Kinoshita, Adran Offthalmoleg, Prifysgol Meddygaeth Talaith Kyoto, Kyoto, Japan
Yr Athro Noriko Koizumi, Canolfan Meddygaeth Adfywiol, Adran Peirianneg Biofeddygol, Prifysgol Doshisha, Kyoto, Japan: Llawfeddygaeth fewnwthiol newydd ar gyfer trin clefyd endothelaidd corneal
Peirianneg meinwe ac arbenigedd atgyweirio
- Uwchstrwythur matricsau meinwe cysylltiol, yn enwedig yn y llygad a'r cymal synovaidd.
- Rhyngweithio colagen a proteoglycans mewn matrics meinwe cysylltiol o gornbilen, sclera, cartilag articular a ligament.
- Rheoleiddio tryloywder cornbilen trwy macromoleciwlau meinwe.
- Datblygu technegau prosesu meinwe tymheredd isel ar gyfer archwilio uwchstrwythur meinwe hydradol.
- Datblygiad Corneal: sefydlu pensaernïaeth lamellar cornbilen gan keratocytes embryonig.
- Datblygu moddau llawfeddygol anfewnwthiol newydd i drin anhwylderau endothelaidd corneal.
- Cymhwyso dulliau microsgopeg electron sganio 3D (SEM) (sy'n canolbwyntio ar trawst ïon ac adran cyfresol awtomataidd-SEM), ar gyfer ailadeiladu matricsau meinwe gyswllt ocwlar 3D.
- Dadansoddiad strwythurol o fatricsau artiffisial ar gyfer amnewid cornbilen."
