![Anthony Hayes PhD BSc (Hons) MSc](https://profiles-cardiff.imgix.net/attachments/5848?w=200&h=200&auto=format&crop=faces&fit=crop&q=90")
Dr Anthony Hayes
PhD BSc (Hons) MSc
Teams and roles for Anthony Hayes
Manager, Bioimaging Research Hub, School of Biosciences
Biosi Administration
Overview
My scientific background and interests are in bioimaging, connective tissue biology and extracellular matrix research.
I have been manager of the Bioimaging Research Hub in its various manifestations since 2005 and have wide expertise in light microscopy, specialising in confocal, lightsheet, and high throughput/high content imaging modalities. I have considerable experience in histology, immunohistochemistry, multiplexed fluorescence labelling, cell culture and molecular biology. I am also an experienced, trained electron microscopist.
I play a key role in bringing the latest cutting edge imaging technologies to Cardiff University, including our latest Zeiss Celldiscoverer7/LSM900 system, and work with partners across the GW4 alliance to coordinate imaging provision, foster collaboration and improve sustainability. I oversee cross-college training and application support of researchers from initial concept through to project completion on all Hub imaging systems and am responsible for maintaining excellent service standards across light microscopy and bioimaging, including implementation of LEAF standards to improve sustainability and efficiency.
Over the last thirty years I have published widely across the biomedical and lifesciences with a particular focus on the development, growth and repair of musculoskeletal connective tissues and the role of extracellular matrix and cell surface proteoglycans in stem cell niches.
I have enjoyed productive research collaborations at local, national and international levels, worked with industrial clients and external organisations, and contributed to BIOSI teaching, including developing several e-learning resources for student pedagogy, microscopy training and science outreach and engagement purposes.
Publication
2024
- Hayes, A. 2024. The 3D Pollen Library Collection at NIH3D: From humble beginnings to the largest open-source collection of online 3D pollen models worldwide. infocus Magazine 76(Dec), pp. 4-17. (10.22443/rms.inf.1.277)
2023
- Hayes, A. J. and Melrose, J. 2023. HS, an ancient molecular recognition and information storage glycosaminoglycan, equips HS-proteoglycans with diverse matrix and cell-interactive properties operative in tissue development and tissue function in health and disease. International Journal of Molecular Sciences 24(2), article number: 1148. (10.3390/ijms24021148)
2022
- Hayes, A. J., Farrugia, B. L., Biose, I. J., Bix, G. J. and Melrose, J. 2022. Perlecan, a multi-functional, cell-instructive, matrix-stabilizing proteoglycan with roles in tissue development has relevance to connective tissue repair and regeneration. Frontiers in Cell and Developmental Biology 10, article number: 856261. (10.3389/fcell.2022.856261)
- Hayes, A. J., Whitelock, J. and Melrose, J. 2022. Regulation of FGF-2, FGF-18 and transcription factor activity by perlecan in the maturational development of transitional rudiment and growth plate cartilages and in the maintenance of permanent cartilage homeostasis. International Journal of Molecular Sciences 23(4), article number: 1934. (10.3390/ijms23041934)
- Bricking, A., Hayes, A. and Madgwick, R. 2022. An interim report on histological analysis of human bones from Fishmonger's Swallet, Gloucestershire. Proceedings of the University of Bristol Speleological Society 29(1), pp. 67-86.
2021
- Hayes, A. J. and Melrose, J. 2021. Neural tissue homeostasis and repair is regulated via CS and DS proteoglycan motifs. Frontiers in Cell and Developmental Biology 9, article number: 696650. (10.3389/fcell.2021.696640)
- Melrose, J., Hayes, A. J. and Bix, G. 2021. The CNS/PNS extracellular matrix provides instructive guidance cues to neural cells and neuroregulatory proteins in neural development and repair. International Journal of Molecular Sciences 22(11), article number: 5583. (10.3390/ijms22115583)
- Hayes, A. J. and Melrose, J. 2021. What are the potential roles of nuclear perlecan and other heparan sulphate proteoglycans in the normal and malignant phenotype. International Journal of Molecular Sciences 22(9), article number: 4415. (10.3390/ijms22094415)
- Guilak, F., Hayes, A. J. and Melrose, J. 2021. Perlecan in pericellular mechanosensory cell-matrix communication, extracellular matrix stabilisation and mechanoregulation of load-bearing connective tissues. International Journal of Molecular Sciences 22(5), article number: 2716. (10.3390/ijms22052716)
- Markov, P., Hayes, A. J., Zhu, H., Boote, C. and Blain, E. J. 2021. 3D immuno-confocal image reconstruction of fibroblast cytoskeleton and nucleus architecture. Journal of Biophotonics 14(1), article number: e202000202. (10.1002/jbio.202000202)
- Hayes, A. J. and Melrose, J. 2021. 3D distribution of perlecan within intervertebral disc chondrons suggests novel regulatory roles for this multifunctional modular heparan sulphate proteoglycan. European Cells and Materials 41, pp. 73-89. (10.22203/eCM.v041a06)
- Ashworth, S. et al. 2021. Chondroitin sulfate as a potential modulator of the stem cell niche in cornea. Frontiers in Cell and Developmental Biology 8, article number: 567358. (10.3389/fcell.2020.567358)
2020
- Hayes, A. J. and Melrose, J. 2020. Electro-stimulation, a promising therapeutic treatment modality for tissue repair: emerging roles of sulfated glycosaminoglycans as electro-regulatory mediators of intrinsic repair processes. Advanced Therapeutics 3(11), article number: 2000151. (10.1002/adtp.202000151)
- Guschina, I. A., Hayes, A. J. and Ormerod, S. J. 2020. Polystyrene microplastics decrease accumulation of essential fatty acids in common freshwater algae. Environmental Pollution 263(PartB), article number: 114425. (10.1016/j.envpol.2020.114425)
- Hayes, A. J. and Melrose, J. 2020. Keratan sulphate in the tumour environment. In: Tumor Microenvironment: Extracellular Matrix Components – Part A., Vol. 1245. Advances in Experimental Medicine and Biology Berlin: Springer-Verlag, pp. 39-66., (10.1007/978-3-030-40146-7_2)
- Hayes, A. J. and Melrose, J. 2020. Immunolocalization of keratan sulfate in rat spinal tissues using the keratanase generated BKS-1(+) neoepitope: correlation of expression patterns with the slass II SLRPs, lumican and keratocan. Cells 9(4), article number: 826. (10.3390/cells9040826)
- Lloyd, D. et al. 2020. Functional imaging of a model unicell: Spironucleus vortens as an anaerobic but aerotolerant flagellated protist. Advances in Microbial Physiology 76, pp. 41-79. (10.1016/bs.ampbs.2020.01.002)
- Hayes, A. J. and Melrose, J. 2020. Aggrecan, the primary weight-bearing cartilage proteoglycan, has context-dependent, cell-directive properties in embryonic development and neurogenesis: aggrecan glycan side chain modifications convey interactive biodiversity. Biomolecules 10(9), article number: 1244. (10.3390/biom10091244)
2019
- Hayes, A. J. and Melrose, J. 2019. Glycosaminoglycan and Proteoglycan Biotherapeutics in Articular Cartilage Protection and Repair Strategies: Novel Approaches to Visco?supplementation in Orthobiologics. Advanced Therapeutics 2(8), article number: 1900034. (10.1002/adtp.201900034)
- Jones, S., Caws, T., Hayes, A., Marsh Durban, V., Corteling, R. and Watson, P. 2019. Characterizing the cellular uptake of neural stem-cell derived exosomes using live-cell imaging techniques. Presented at: ISEV2019 Annual Meeting, Kyoto, Japan, 24-28 April 2019, Vol. 8. Vol. sup1. Wiley pp. IP12., (10.1080/20013078.2019.1593587)
- Groves, L. M. et al. 2019. Fluorescent functionalised naphthalimides and their Au(i)–NHC complexes for potential use in cellular bioimaging. Dalton Transactions 48(5), pp. 1599-1612. (10.1039/C8DT04069A)
2018
- Hayes, A. J., Smith, S. M., Caterson, B. and Melrose, J. 2018. Concise Review: Stem/progenitor cell proteoglycans decorated with 7-D-4, 4-C-3, and 3-B-3(-) chondroitin sulfate motifs are morphogenetic markers of tissue development. STEM CELLS 36(10), pp. 1475-1486. (10.1002/stem.2860)
- Hayes, A. J. and Melrose, J. 2018. Glycans and glycosaminoglycans in neurobiology: key regulators of neuronal cell function and fate. Biochemical Journal 475(15), pp. 2511-2545. (10.1042/BCJ20180283)
- Hallett, A. J. et al. 2018. Exploring the cellular uptake and localisation of phosphorescent rhenium fac-tricarbonyl metallosurfactants as a function of lipophilicity. Dalton Transactions 47(40), pp. 14241-14253. (10.1039/C8DT00669E)
- Hayes, A., Sugahara, K., Farrugia, B., Whitelock, J. M., Caterson, B. and Melrose, J. 2018. Biodiversity of CS-proteoglycan sulphation motifs: chemical messenger recognition modules with roles in information transfer, control of cellular behaviour and tissue morphogenesis. Biochemical Journal 475(3), pp. 587-620. (10.1042/BCJ20170820)
- Zaki, A. et al. 2018. Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle. RSC Advances 8, pp. 5768-5775. (10.1039/c7ra11166e)
2017
- Langdon-Jones, E. E. et al. 2017. Luminescent 1,8-Naphthalimide-Derived ReI Complexes: syntheses, spectroscopy, X-ray structure and preliminary bioimaging in fission yeast cells. European Journal of Inorganic Chemistry 2017(44), pp. 5279-5287. (10.1002/ejic.201700549)
- Perry, I. et al. 2017. Production of 3D printed scale models from microscope volume datasets for use in STEM education. EMS Engineering Science Journal 1(1), article number: 2.
- Sands, K. M. et al. 2017. Respiratory pathogen colonisation of dental plaque, the lower airways and endotracheal tube biofilms during mechanical ventilation. Journal of Critical Care 37, pp. 30-37. (10.1016/j.jcrc.2016.07.019)
- Pope, S. J. A., Langdon-Jones, E., Jones, A., Williams, C. F., Hayes, A. J., Lloyd, D. and Mottram, H. J. 2017. Anticancer, azonafide-inspired fluorescent ligands and their rhenium(I) complexes for cellular imaging. European Journal of Inorganic Chemistry 2017(3), pp. 759-766. (10.1002/ejic.201601271)
2016
- Hayes, A. J., Shu, C. C., Lord, M. S., Little, C. B., Whitelock, J. M. and Melrose, J. 2016. Pericellular colocalisation and interactive properties of type VI collagen and perlecan in the intervertebral disc. European Cells and Materials 32, pp. 40-57. (10.22203/eCM.v032a03)
- Hayes, A. J., Hughes, C. E., Smith, S. M., Caterson, B., Little, C. B. and Melrose, J. 2016. The CS sulphation motifs 4C3, 7D4, 3B3[-]; and perlecan identify stem cell populations and niches, activated progenitor cells and transitional tissue development in the fetal human elbow. Stem Cells and Development 25(11), pp. 836-847. (10.1089/scd.2016.0054)
- Kille, P. et al. 2016. Venus trapped, Mars transits: Cu and Fe redox chemistry, cellular topography, and in situ ligand binding in terrestrial isopod hepatopancreas. Open Biology Journal 6(3), pp. -., article number: 150270. (10.1098/rsob.150270)
2015
- Beckett, M. C., Ralphs, J. R., Caterson, B. and Hayes, A. J. 2015. The transmembrane heparan sulphate proteoglycan syndecan-4 is involved in establishment of the lamellar structure of the annulus fibrosus of the intervertebral disc. European Cells and Materials 30, pp. 69-88.
- Stacey, O. J. et al. 2015. Water soluble, cyclometalated Pt(II)–Ln(III) conjugates towards novel bimodal imaging agents. Chemical Communications 51(61), pp. 12305-12308. (10.1039/C5CC02623G)
- Langdon-Jones, E. E. et al. 2015. Alkynyl-naphthalimide fluorophores: gold coordination chemistry and cellular imaging applications. Inorganic Chemistry 54(13), pp. 6606-6615., article number: 150618152106000. (10.1021/acs.inorgchem.5b00954)
- Lloyd, D., Lewis, I. B., Williams, C. F., Hayes, A. J., Symons, H. and Hill, E. C. 2015. Motility of the diplomonad fish parasite Spironucleus vortens through thixotropic solid media. Microbiology 161(1), pp. 213-218. (10.1099/mic.0.082529-0)
2014
- Boros-Majewska, J. et al. 2014. Novel Nystatin A1 derivatives exhibiting low host cell toxicity and antifungal activity in an in vitro model of oral candidosis. Medical Microbiology and Immunology 203(5), pp. 341-355. (10.1007/s00430-014-0343-4)
- Sivan, S. S. et al. 2014. Biochemical composition and turnover of the extracellular matrix of the normal and degenerate intervertebral disc. European Spine Journal 23(3), pp. 344-353. (10.1007/s00586-013-2767-8)
- Hayes, A. J., Gibson, M. A., Shu, C. and Melrose, J. 2014. Confocal microscopy demonstrates association of LTBP-2 in fibrillin-1 microfibrils and colocalisation with perlecan in the disc cell pericellular matrix. Tissue and Cell 46(3), pp. 185-197. (10.1016/j.tice.2014.04.002)
- Langdon-Jones, E. E. et al. 2014. Fluorescent rhenium-naphthalimide conjugates as cellular imaging agents. Inorganic Chemistry 53(7), pp. 3788-3797. (10.1021/ic500142z)
- Lloyd, D. et al. 2014. Intracellular oxygen: similar results from two methods of measurement using Phosphorescent nanoparticles. Journal of Innovative Optical Health Sciences 7(2), article number: 1350041. (10.1142/S1793545813500417)
2013
- Shu, C. et al. 2013. The ovine newborn and human foetal intervertebral disc contain perlecan and aggrecan variably substituted with native 7D4 CS sulphation motif: spatiotemporal immunolocalisation and co-distribution with Notch-1 in the human foetal disc. Glycoconjugate Journal 30(7), pp. 717-725. (10.1007/s10719-013-9475-9)
- Millet, C. O. M., Williams, C. F., Hayes, A. J., Hann, A. C., Cable, J. and Lloyd, D. 2013. Mitochondria-derived organelles in the diplomonad fish parasite Spironucleus vortens. Experimental Parasitology 135(2), pp. 262-273. (10.1016/j.exppara.2013.07.003)
- Hayes, A. J. et al. 2013. Spinal deformity in aged zebrafish Is accompanied by degenerative changes to their vertebrae that resemble osteoarthritis. PLoS ONE 8(9), pp. e75787. (10.1371/journal.pone.0075787)
- Williams, C. F., Millet, C. O., Hayes, A. J., Cable, J. and Lloyd, D. 2013. Diversity in mitochondrion-derived organelles of the parasitic diplomonads Spironucleus and Giardia. Trends in Parasitology 29(7), pp. 311-312. (10.1016/j.pt.2013.04.004)
- Hayes, A. J., Mitchell, R. E., Bashford, A., Reynolds, S., Caterson, B. and Hammond, C. L. 2013. Expression of glycosaminoglycan epitopes during zebrafish skeletogenesis. Developmental Dynamics 242(6), pp. 778-789. (10.1002/dvdy.23970)
- Li, S., Hayes, A. J., Caterson, B. and Hughes, C. E. 2013. The effect of beta-xylosides on the chondrogenic differentiation of mesenchymal stem cells. Histochemistry and Cell Biology 139(1), pp. 59-74. (10.1007/s00418-012-1017-1)
- Hayes, A. J., Smith, S. M. and Melrose, J. 2013. Comparative immunolocalisation of fibrillin-1 and perlecan in the human foetal, and HS-deficient hspg2 exon 3 null mutant mouse intervertebral disc. Histochemistry and Cell Biology 139(1), pp. 1-11. (10.1007/s00418-012-1041-1)
2012
- Hooper, S. J., Percival, S. L., Hill, K. E., Thomas, D. W., Hayes, A. J. and Williams, D. W. 2012. The visualisation and speed of kill of wound isolates on a silver alginate dressing. International Wound Journal 9(6), pp. 633-642. (10.1111/j.1742-481X.2012.00927.x)
- Melrose, J., Isaacs, M. D., Smith, S. M., Hughes, C. E., Little, C. B., Caterson, B. and Hayes, A. J. 2012. Chondroitin sulphate and heparan sulphate sulphation motifs and their proteoglycans are involved in articular cartilage formation during human foetal knee joint development. Histochemistry and Cell Biology 138(3), pp. 461-475. (10.1007/s00418-012-0968-6)
- Taylor, D. W., Ahmed, N., Hayes, A. J., Ferguson, P., Gross, A. E., Caterson, B. and Kandel, R. A. 2012. Hyaline cartilage tissue is formed through the co-culture of passaged human chondrocytes and primary bovine chondrocytes. Journal of Histochemistry & Cytochemistry 60(8), pp. 576-587. (10.1369/0022155412449018)
2011
- Parsons, P. et al. 2011. Type IX collagen interacts with fibronectin providing an important molecular bridge in articular cartilage. Journal of Biological Chemistry 286(40), pp. 34986-34997. (10.1074/jbc.M111.238188)
- Evans, L. C., Williams, A. S., Hayes, A. J., Jones, S. A. and Nowell, M. A. 2011. Suppression of leukocyte infiltration and cartilage degradation by selective inhibition of pre-B cell colony-enhancing factor/visfatin/nicotinamide phosphoribosyltransferase: Apo866-mediated therapy in human fibroblasts and murine collagen-induced arthritis. Arthritis and Rheumatism 63(7), pp. 1866-1877. (10.1002/art.30338)
- Evans, A., Williams, A. S., Hayes, A. J. and Nowell, M. A. 2011. Investigating the role of PBEF on cartilage integrity [Abstract]. International Journal of Experimental Pathology 92(3), pp. A33-A34. (10.1111/j.1365-2613.2010.00759.x)
- Akhtar, S., Bron, A., Hayes, A. J., Meek, K. M. A. and Caterson, B. 2011. Role of keratan sulphate (sulphated poly -N-acetyllactosamine repeats) in keratoconic cornea, histochemical, and ultrastructural analysis. Graefe's Archive for Clinical and Experimental Ophthalmology 249(3), pp. 413-420. (10.1007/s00417-010-1512-9)
- Hayes, A. J., Hughes, C. E., Ralphs, J. R. and Caterson, B. 2011. Chondroitin sulphate sulphation motif expression in the ontogeny of the intervertebral disc. European Cells and Materials 21, pp. 1-14.
- Silva, S., Henriques, M. C., Hayes, A. J., Oliveira, R., Azeredo, J. and Williams, D. W. 2011. Candida glabrata and Candida albicans co-infection of an in vitro oral epithelium. Journal of Oral Pathology and Medicine 40(5), pp. 421-427. (10.1111/j.1600-0714.2010.00981.x)
- Hayes, A. J. and Ralphs, J. R. 2011. The response of foetal annulus fibrosus cells to growth factors: modulation of matrix synthesis by TGF-β1 and IGF-1. Histochemistry and Cell Biology 136(2), pp. 163-175. (10.1007/s00418-011-0835-x)
- Thorp-Greenwood, F. L. et al. 2011. A 'Sleeping Trojan Horse' which transports metal ions into cells, localises in nucleoli, and has potential for bimodal fluorescence/PET imaging. Chemical Communications 47(11), pp. 3096-3098. (10.1039/c1cc10141b)
- Hayes, A. J., Isaacs, M. D., Hughes, C. E., Caterson, B. and Ralphs, J. R. 2011. Collagen fibrillogenesis in the development of the annulus fibrosus of the intervertebral disc. European Cells and Materials 22, pp. 226-241.
- Hayes, A. J., Lord, M. S., Smith, S. M., Smith, M. M., Whitelock, J. M., Weiss, A. S. and Melrose, J. 2011. Colocalization in vivo and association in vitro of perlecan and elastin. Histochemistry and Cell Biology 136(4), pp. 437-454. (10.1007/s00418-011-0854-7)
- Hayes, A. J., Smith, S. M., Gibson, M. A. and Melrose, J. 2011. Comparative immunolocalization of the elastin fiber-associated proteins fibrillin-1, LTBP-2, and MAGP-1 With components of the collagenous and proteoglycan matrix of the fetal human intervertebral disc. Spine 36(21), pp. E1365-E1372. (10.1097/BRS.0b013e31821fd23e)
2010
- Coogan, M. P. et al. 2010. Probing intracellular oxygen by quenched phosphorescence lifetimes of nanoparticles containing polyacrylamide-embedded [Ru(dpp(SO3Na)2)3]Cl2. Photochemical and Photobiological Sciences 9(1), pp. 103-109. (10.1039/b9pp00071b)
- Fernández-Moreira, V. et al. 2010. Uptake and localisation of rhenium fac-tricarbonyl polypyridyls in fluorescent cell imaging experiments. Organic and Biomolecular Chemistry 8(17), pp. 3888-3901. (10.1039/c004610h)
- Ralphs, J. R., Caterson, B., Hughes, C. E. and Hayes, A. J. 2010. Chondroitin sulphate sulfation motifs in intervertebral disc development [Abstract]. European Cells and Materials 20(Supp 2), pp. 3.
2009
- Gealy, E. C., Hayes, A. J., Buckwell, R., Young, R. D., Caterson, B., Quantock, A. J. and Ralphs, J. R. 2009. Actin and type I collagen propeptide distribution in the developing chick cornea. Investigative Ophthalmology & Visual Science 50(4), pp. 1653 -1658. (10.1167/iovs.08-2554)
- Nowell, M. A. et al. 2009. Therapeutic targeting of IL-6 trans signaling counteracts STAT3 control of experimental inflammatory arthritis. Journal of Immunology 182(1), pp. 613-622.
- Malic, S., Hill, K. E., Hayes, A. J., Percival, S. L., Thomas, D. W. and Williams, D. W. 2009. Detection and identification of specific bacteria in wound biofilms using peptide nucleic acid fluorescent in situ hybridization (PNA FISH). Microbiology-Sgm 155, pp. 2603-2611. (10.1099/mic.0.028712-0)
2008
- Hayes, A. J., Hughes, C. E. and Caterson, B. 2008. Antibodies and immunohistochemistry in extracellular matrix research. Methods 45(1), pp. 10-21. (10.1016/j.ymeth.2008.01.011)
- Melrose, J., Hayes, A. J., Whitelock, J. M. and Little, C. B. 2008. Perlecan, the 'jack of all trades' proteoglycan of cartilaginous weight-bearing connective tissues. Bioessays 30(5), pp. 457-469. (10.1002/bies.20748)
- Amoroso, A. J. et al. 2008. 3-Chloromethylpyridyl bipyridine fac-tricarbonyl rhenium: a thiol-reactive luminophore for fluorescence microscopy accumulates in mitochondria. New Journal of Chemistry 32(7), pp. 1097-1102. (10.1039/b802215a)
- Hayes, A. J., Tudor, D., Nowell, M. A., Caterson, B. and Hughes, C. E. 2008. Chondroitin sulfate sulfation motifs as putative biomarkers for isolation of articular cartilage progenitor cells. Journal of Histochemistry & Cytochemistry 56(2), pp. 125-38. (10.1369/jhc.7A7320.2007)
- Akhtar, S., Kerr, B., Hayes, A. J., Hughes, C. E., Meek, K. M. and Caterson, B. 2008. Immunochemical localisation of keratan sulphate proteoglycans in cornea, sclera and limbus using a keratanase generated neoepitope monoclonal antibody. Investigative Ophthalmology and Visual Science 49(6), pp. 2424-2431. (10.1167/iovs.06-1498)
- Bazou, D., Coakley, W. T., Hayes, A. J. and Jackson, S. K. 2008. Long-term viability and proliferation of alginate-encapsulated 3-D HepG2 aggregates formed in an ultrasound trap. Toxicology in Vitro 22(5), pp. 1321-1331. (10.1016/j.tiv.2008.03.014)
- Caterson, B., Hayes, A. J., Tudor, D., Nowell, M. A. and Hughes, C. E. 2008. Chondroitin sulphate motifs as biomarkers for the stem/progenitor cell niche in musculoskeletal tissues. European Cells and Materials 16(Supp 2), pp. 47-47.
2007
- Amoroso, A. J. et al. 2007. Rhenium fac tricarbonyl bisimine complexes: biologically useful fluorochromes for cell imaging applications. Chemical Communications(29), pp. 3066-3068. (10.1039/b706657k)
- Malic, S., Hill, K. E., Ralphs, J. R., Hayes, A. J., Thomas, D. W., Potts, A. J. C. and Williams, D. W. 2007. Characterisation of Candida albicans infection of an in vitro oral epithelial model using confocal laser scanning microscopy. Oral Microbiology And Immunolology 22(3), pp. 188-194. (10.1111/j.1399-302X.2007.00344.x)
- Hayes, A. J., Hall, A., Brown, L., Tubo, R. and Caterson, B. 2007. Macromolecular organization and in vitro growth characteristics of scaffold-free neocartilage grafts. Journal of Histochemistry & Cytochemistry 55(8), pp. 853-866. (10.1369/jhc.7A7210.2007)
- Gealy, E. C. et al. 2007. Differential expression of the keratan sulphate proteoglycan, keratocan, during chick corneal embryogenesis. Histochemistry and Cell Biology 128(6), pp. 551-555. (10.1007/s00418-007-0332-4)
- Aon, M. A., Cortassa, S., Lemar, K. M., Hayes, A. J. and Lloyd, D. 2007. Single and cell population respiratory oscillations in yeast: a 2-photon scanning laser microscopy study. FEBS Letters 581(1), pp. 8-14. (10.1016/j.febslet.2006.11.068)
2006
- Blain, E. J., Gilbert, S. J., Hayes, A. J. and Duance, V. C. 2006. Disassembly of the vimentin cytoskeleton disrupts articular cartilage chondrocyte homeostasis.. Matrix biology 25(7), pp. 398-408. (10.1016/j.matbio.2006.06.002)
- Reed, K. R., Sansom, O. J., Hayes, A. J., Gescher, A. J., Peters, J. M. and Clarke, A. R. 2006. PPARδ status and mismatch repair mediated neoplasia in the mouse intestine. BMC Cancer 6(113) (10.1186/1471-2407-6-113)
2005
- Hall, A. K., Hayes, A. J., Brown, L., Tubo, R. and Caterson, B. 2005. Tissue-engineered cartilage grafts for use in cartilage repair technologies [Abstract]. International Journal of Experimental Pathology 86(3), pp. A9. (10.1111/j.0959-9673.2005.00426.x)
- Young, R. D. et al. 2005. Atypical composition and ultrastructure of proteoglycans in the mouse corneal stroma. Investigative Ophthalmology and Visual Science 46(6), pp. 1973-1978. (10.1167/iovs.04-1309)
- Hayes, A. J., Tudor, D., Nowell, M. A., Hughes, C. E. and Caterson, B. 2005. Novel chondroitin sulphation motifs as putative biomarkers of articular cartilage chondroprogenitor cells [Abstract]. European Cells and Materials 10(Supp 2), pp. 72.
2004
- Sansom, O. J. et al. 2004. Loss of Apc in vivo immediately perturbs Wnt signaling, differentiation, and migration. Genes and Development 18(12), pp. 1385-1390. (10.1101/gad.287404)
- Reed, K. R., Sansom, O. J., Hayes, A. J., Gescher, A. J., Winton, D. J., Peters, J. M. and Clarke, A. R. 2004. PPARδ status and Apc-mediated tumourigenesis in the mouse intestine. Oncogene 23(55), pp. 8992-8996. (10.1038/sj.onc.1208143)
2003
- Hayes, A. J., Dowthwaite, G. P., Webster, S. V. and Archer, C. W. 2003. The distribution of Notch receptors and their ligands during articular cartilage development. Journal of Anatomy 202(6), pp. 495-502. (10.1046/j.1469-7580.2003.00185.x)
2001
- Hayes, A. J., Benjamin, M. and Ralphs, J. R. 2001. Extracellular matrix in development of the intervertebral disc. Matrix Biology 20(2), pp. 107-121. (10.1016/S0945-053X(01)00125-1)
- Hayes, A., MacPherson, S., Morrison, H., Dowthwaite, G. and Archer, C. 2001. The development of articular cartilage: Evidence for an appositional growth mechanism. Anatomy and Embryology 203(6), pp. 469-479. (10.1007/s004290100178)
1999
- Hayes, A. J., Benjamin, M. and Ralphs, J. R. 1999. Role of actin stress fibres in the development of the intervertebral disc: cytoskeletal control of extracellular matrix assembly. Developmental Dynamics 215(3), pp. 179-189. (10.1002/(SICI)1097-0177(199907)215:3<179::AID-AJA1>3.0.CO;2-Q)
- Lloyd, D., Hayes, A. J. and Ralphs, J. R. 1999. Confocal laser scanning microscopy of environmental samples. In: Edwards, C. ed. Environmental Monitoring of Bacteria., Vol. 12. USA: Springer, pp. 251-265., (10.1385/0-89603-566-2:251)
- Williams, I. et al. 1999. Flow cytometry and other techniques show that Staphylococcus aureus undergoes significant physiological changes in the early stages of surface-attached culture. Microbiology 145(6), pp. 1325-1333. (10.1099/13500872-145-6-1325)
1998
- Lloyd, D. et al. 1998. Micro-ecology of peat: minimally invasive analysis using confocal laser scanning microscopy, membrane inlet mass spectrometry and PCR amplification of methanogen-specific gene sequences. FEMS Microbiology Ecology 25(2), pp. 179-188. (10.1016/S0168-6496(97)00094-9)
- Shnyder, S. D., Hayes, A., Pringle, J. and Archer, C. 1998. P-glycoprotein and metallothionein expression and resistance to chemotherapy in osteosarcoma. British Journal of Cancer 78, pp. 757-759. (10.1038/bjc.1998.573)
1997
- Biagini, G. A., Hayes, A. J., Suller, M. T. E., Winters, C., Finlay, B. J. and Archer, C. W. 1997. Hydrogenosomes of metopus contortus physiologically resemble mitochondria. Microbiology 143(5), pp. 1623-1629. (10.1099/00221287-143-5-1623)
- Hayes, A. J., Ralphs, J. R. and Benjamin, M. 1997. The involvement of actin in annulus formation in the rat lumbar intervertebral disc. Journal of Anatomy 191(1), pp. 125.
1996
- Lloyd, D., Moran, C. A., Suller, M. T. E., Dinsdale, M. G. and Hayes, A. J. 1996. Flow cytometric monitoring of rhodamine 123 and a cyanine dye uptake by yeast during cider fermentation. Journal of the Institute of Brewing 102(4), pp. 251-259. (10.1002/j.2050-0416.1996.tb00910.x)
- Lloyd, D., Williams, A. G., Amann, R., Hayes, A. J., Durrant, L. and Ralphs, J. R. 1996. Intracellular prokaryotes in rumen ciliate protozoa: detection by confocal laser scanning microscopy after in situ hybridization with fluorescent 16S rRNA probes. European Journal of Protistology 32(4), pp. 523-531. (10.1016/S0932-4739(96)80011-3)
1995
- Lloyd, D. and Hayes, A. J. 1995. Vigour, vitality and viability of microorganisms. FEMS Microbiology Letters 133(1-2), pp. 1-7. (10.1111/j.1574-6968.1995.tb07852.x)
Articles
- Hayes, A. 2024. The 3D Pollen Library Collection at NIH3D: From humble beginnings to the largest open-source collection of online 3D pollen models worldwide. infocus Magazine 76(Dec), pp. 4-17. (10.22443/rms.inf.1.277)
- Hayes, A. J. and Melrose, J. 2023. HS, an ancient molecular recognition and information storage glycosaminoglycan, equips HS-proteoglycans with diverse matrix and cell-interactive properties operative in tissue development and tissue function in health and disease. International Journal of Molecular Sciences 24(2), article number: 1148. (10.3390/ijms24021148)
- Hayes, A. J., Farrugia, B. L., Biose, I. J., Bix, G. J. and Melrose, J. 2022. Perlecan, a multi-functional, cell-instructive, matrix-stabilizing proteoglycan with roles in tissue development has relevance to connective tissue repair and regeneration. Frontiers in Cell and Developmental Biology 10, article number: 856261. (10.3389/fcell.2022.856261)
- Hayes, A. J., Whitelock, J. and Melrose, J. 2022. Regulation of FGF-2, FGF-18 and transcription factor activity by perlecan in the maturational development of transitional rudiment and growth plate cartilages and in the maintenance of permanent cartilage homeostasis. International Journal of Molecular Sciences 23(4), article number: 1934. (10.3390/ijms23041934)
- Bricking, A., Hayes, A. and Madgwick, R. 2022. An interim report on histological analysis of human bones from Fishmonger's Swallet, Gloucestershire. Proceedings of the University of Bristol Speleological Society 29(1), pp. 67-86.
- Hayes, A. J. and Melrose, J. 2021. Neural tissue homeostasis and repair is regulated via CS and DS proteoglycan motifs. Frontiers in Cell and Developmental Biology 9, article number: 696650. (10.3389/fcell.2021.696640)
- Melrose, J., Hayes, A. J. and Bix, G. 2021. The CNS/PNS extracellular matrix provides instructive guidance cues to neural cells and neuroregulatory proteins in neural development and repair. International Journal of Molecular Sciences 22(11), article number: 5583. (10.3390/ijms22115583)
- Hayes, A. J. and Melrose, J. 2021. What are the potential roles of nuclear perlecan and other heparan sulphate proteoglycans in the normal and malignant phenotype. International Journal of Molecular Sciences 22(9), article number: 4415. (10.3390/ijms22094415)
- Guilak, F., Hayes, A. J. and Melrose, J. 2021. Perlecan in pericellular mechanosensory cell-matrix communication, extracellular matrix stabilisation and mechanoregulation of load-bearing connective tissues. International Journal of Molecular Sciences 22(5), article number: 2716. (10.3390/ijms22052716)
- Markov, P., Hayes, A. J., Zhu, H., Boote, C. and Blain, E. J. 2021. 3D immuno-confocal image reconstruction of fibroblast cytoskeleton and nucleus architecture. Journal of Biophotonics 14(1), article number: e202000202. (10.1002/jbio.202000202)
- Hayes, A. J. and Melrose, J. 2021. 3D distribution of perlecan within intervertebral disc chondrons suggests novel regulatory roles for this multifunctional modular heparan sulphate proteoglycan. European Cells and Materials 41, pp. 73-89. (10.22203/eCM.v041a06)
- Ashworth, S. et al. 2021. Chondroitin sulfate as a potential modulator of the stem cell niche in cornea. Frontiers in Cell and Developmental Biology 8, article number: 567358. (10.3389/fcell.2020.567358)
- Hayes, A. J. and Melrose, J. 2020. Electro-stimulation, a promising therapeutic treatment modality for tissue repair: emerging roles of sulfated glycosaminoglycans as electro-regulatory mediators of intrinsic repair processes. Advanced Therapeutics 3(11), article number: 2000151. (10.1002/adtp.202000151)
- Guschina, I. A., Hayes, A. J. and Ormerod, S. J. 2020. Polystyrene microplastics decrease accumulation of essential fatty acids in common freshwater algae. Environmental Pollution 263(PartB), article number: 114425. (10.1016/j.envpol.2020.114425)
- Hayes, A. J. and Melrose, J. 2020. Immunolocalization of keratan sulfate in rat spinal tissues using the keratanase generated BKS-1(+) neoepitope: correlation of expression patterns with the slass II SLRPs, lumican and keratocan. Cells 9(4), article number: 826. (10.3390/cells9040826)
- Lloyd, D. et al. 2020. Functional imaging of a model unicell: Spironucleus vortens as an anaerobic but aerotolerant flagellated protist. Advances in Microbial Physiology 76, pp. 41-79. (10.1016/bs.ampbs.2020.01.002)
- Hayes, A. J. and Melrose, J. 2020. Aggrecan, the primary weight-bearing cartilage proteoglycan, has context-dependent, cell-directive properties in embryonic development and neurogenesis: aggrecan glycan side chain modifications convey interactive biodiversity. Biomolecules 10(9), article number: 1244. (10.3390/biom10091244)
- Hayes, A. J. and Melrose, J. 2019. Glycosaminoglycan and Proteoglycan Biotherapeutics in Articular Cartilage Protection and Repair Strategies: Novel Approaches to Visco?supplementation in Orthobiologics. Advanced Therapeutics 2(8), article number: 1900034. (10.1002/adtp.201900034)
- Groves, L. M. et al. 2019. Fluorescent functionalised naphthalimides and their Au(i)–NHC complexes for potential use in cellular bioimaging. Dalton Transactions 48(5), pp. 1599-1612. (10.1039/C8DT04069A)
- Hayes, A. J., Smith, S. M., Caterson, B. and Melrose, J. 2018. Concise Review: Stem/progenitor cell proteoglycans decorated with 7-D-4, 4-C-3, and 3-B-3(-) chondroitin sulfate motifs are morphogenetic markers of tissue development. STEM CELLS 36(10), pp. 1475-1486. (10.1002/stem.2860)
- Hayes, A. J. and Melrose, J. 2018. Glycans and glycosaminoglycans in neurobiology: key regulators of neuronal cell function and fate. Biochemical Journal 475(15), pp. 2511-2545. (10.1042/BCJ20180283)
- Hallett, A. J. et al. 2018. Exploring the cellular uptake and localisation of phosphorescent rhenium fac-tricarbonyl metallosurfactants as a function of lipophilicity. Dalton Transactions 47(40), pp. 14241-14253. (10.1039/C8DT00669E)
- Hayes, A., Sugahara, K., Farrugia, B., Whitelock, J. M., Caterson, B. and Melrose, J. 2018. Biodiversity of CS-proteoglycan sulphation motifs: chemical messenger recognition modules with roles in information transfer, control of cellular behaviour and tissue morphogenesis. Biochemical Journal 475(3), pp. 587-620. (10.1042/BCJ20170820)
- Zaki, A. et al. 2018. Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle. RSC Advances 8, pp. 5768-5775. (10.1039/c7ra11166e)
- Langdon-Jones, E. E. et al. 2017. Luminescent 1,8-Naphthalimide-Derived ReI Complexes: syntheses, spectroscopy, X-ray structure and preliminary bioimaging in fission yeast cells. European Journal of Inorganic Chemistry 2017(44), pp. 5279-5287. (10.1002/ejic.201700549)
- Perry, I. et al. 2017. Production of 3D printed scale models from microscope volume datasets for use in STEM education. EMS Engineering Science Journal 1(1), article number: 2.
- Sands, K. M. et al. 2017. Respiratory pathogen colonisation of dental plaque, the lower airways and endotracheal tube biofilms during mechanical ventilation. Journal of Critical Care 37, pp. 30-37. (10.1016/j.jcrc.2016.07.019)
- Pope, S. J. A., Langdon-Jones, E., Jones, A., Williams, C. F., Hayes, A. J., Lloyd, D. and Mottram, H. J. 2017. Anticancer, azonafide-inspired fluorescent ligands and their rhenium(I) complexes for cellular imaging. European Journal of Inorganic Chemistry 2017(3), pp. 759-766. (10.1002/ejic.201601271)
- Hayes, A. J., Shu, C. C., Lord, M. S., Little, C. B., Whitelock, J. M. and Melrose, J. 2016. Pericellular colocalisation and interactive properties of type VI collagen and perlecan in the intervertebral disc. European Cells and Materials 32, pp. 40-57. (10.22203/eCM.v032a03)
- Hayes, A. J., Hughes, C. E., Smith, S. M., Caterson, B., Little, C. B. and Melrose, J. 2016. The CS sulphation motifs 4C3, 7D4, 3B3[-]; and perlecan identify stem cell populations and niches, activated progenitor cells and transitional tissue development in the fetal human elbow. Stem Cells and Development 25(11), pp. 836-847. (10.1089/scd.2016.0054)
- Kille, P. et al. 2016. Venus trapped, Mars transits: Cu and Fe redox chemistry, cellular topography, and in situ ligand binding in terrestrial isopod hepatopancreas. Open Biology Journal 6(3), pp. -., article number: 150270. (10.1098/rsob.150270)
- Beckett, M. C., Ralphs, J. R., Caterson, B. and Hayes, A. J. 2015. The transmembrane heparan sulphate proteoglycan syndecan-4 is involved in establishment of the lamellar structure of the annulus fibrosus of the intervertebral disc. European Cells and Materials 30, pp. 69-88.
- Stacey, O. J. et al. 2015. Water soluble, cyclometalated Pt(II)–Ln(III) conjugates towards novel bimodal imaging agents. Chemical Communications 51(61), pp. 12305-12308. (10.1039/C5CC02623G)
- Langdon-Jones, E. E. et al. 2015. Alkynyl-naphthalimide fluorophores: gold coordination chemistry and cellular imaging applications. Inorganic Chemistry 54(13), pp. 6606-6615., article number: 150618152106000. (10.1021/acs.inorgchem.5b00954)
- Lloyd, D., Lewis, I. B., Williams, C. F., Hayes, A. J., Symons, H. and Hill, E. C. 2015. Motility of the diplomonad fish parasite Spironucleus vortens through thixotropic solid media. Microbiology 161(1), pp. 213-218. (10.1099/mic.0.082529-0)
- Boros-Majewska, J. et al. 2014. Novel Nystatin A1 derivatives exhibiting low host cell toxicity and antifungal activity in an in vitro model of oral candidosis. Medical Microbiology and Immunology 203(5), pp. 341-355. (10.1007/s00430-014-0343-4)
- Sivan, S. S. et al. 2014. Biochemical composition and turnover of the extracellular matrix of the normal and degenerate intervertebral disc. European Spine Journal 23(3), pp. 344-353. (10.1007/s00586-013-2767-8)
- Hayes, A. J., Gibson, M. A., Shu, C. and Melrose, J. 2014. Confocal microscopy demonstrates association of LTBP-2 in fibrillin-1 microfibrils and colocalisation with perlecan in the disc cell pericellular matrix. Tissue and Cell 46(3), pp. 185-197. (10.1016/j.tice.2014.04.002)
- Langdon-Jones, E. E. et al. 2014. Fluorescent rhenium-naphthalimide conjugates as cellular imaging agents. Inorganic Chemistry 53(7), pp. 3788-3797. (10.1021/ic500142z)
- Lloyd, D. et al. 2014. Intracellular oxygen: similar results from two methods of measurement using Phosphorescent nanoparticles. Journal of Innovative Optical Health Sciences 7(2), article number: 1350041. (10.1142/S1793545813500417)
- Shu, C. et al. 2013. The ovine newborn and human foetal intervertebral disc contain perlecan and aggrecan variably substituted with native 7D4 CS sulphation motif: spatiotemporal immunolocalisation and co-distribution with Notch-1 in the human foetal disc. Glycoconjugate Journal 30(7), pp. 717-725. (10.1007/s10719-013-9475-9)
- Millet, C. O. M., Williams, C. F., Hayes, A. J., Hann, A. C., Cable, J. and Lloyd, D. 2013. Mitochondria-derived organelles in the diplomonad fish parasite Spironucleus vortens. Experimental Parasitology 135(2), pp. 262-273. (10.1016/j.exppara.2013.07.003)
- Hayes, A. J. et al. 2013. Spinal deformity in aged zebrafish Is accompanied by degenerative changes to their vertebrae that resemble osteoarthritis. PLoS ONE 8(9), pp. e75787. (10.1371/journal.pone.0075787)
- Williams, C. F., Millet, C. O., Hayes, A. J., Cable, J. and Lloyd, D. 2013. Diversity in mitochondrion-derived organelles of the parasitic diplomonads Spironucleus and Giardia. Trends in Parasitology 29(7), pp. 311-312. (10.1016/j.pt.2013.04.004)
- Hayes, A. J., Mitchell, R. E., Bashford, A., Reynolds, S., Caterson, B. and Hammond, C. L. 2013. Expression of glycosaminoglycan epitopes during zebrafish skeletogenesis. Developmental Dynamics 242(6), pp. 778-789. (10.1002/dvdy.23970)
- Li, S., Hayes, A. J., Caterson, B. and Hughes, C. E. 2013. The effect of beta-xylosides on the chondrogenic differentiation of mesenchymal stem cells. Histochemistry and Cell Biology 139(1), pp. 59-74. (10.1007/s00418-012-1017-1)
- Hayes, A. J., Smith, S. M. and Melrose, J. 2013. Comparative immunolocalisation of fibrillin-1 and perlecan in the human foetal, and HS-deficient hspg2 exon 3 null mutant mouse intervertebral disc. Histochemistry and Cell Biology 139(1), pp. 1-11. (10.1007/s00418-012-1041-1)
- Hooper, S. J., Percival, S. L., Hill, K. E., Thomas, D. W., Hayes, A. J. and Williams, D. W. 2012. The visualisation and speed of kill of wound isolates on a silver alginate dressing. International Wound Journal 9(6), pp. 633-642. (10.1111/j.1742-481X.2012.00927.x)
- Melrose, J., Isaacs, M. D., Smith, S. M., Hughes, C. E., Little, C. B., Caterson, B. and Hayes, A. J. 2012. Chondroitin sulphate and heparan sulphate sulphation motifs and their proteoglycans are involved in articular cartilage formation during human foetal knee joint development. Histochemistry and Cell Biology 138(3), pp. 461-475. (10.1007/s00418-012-0968-6)
- Taylor, D. W., Ahmed, N., Hayes, A. J., Ferguson, P., Gross, A. E., Caterson, B. and Kandel, R. A. 2012. Hyaline cartilage tissue is formed through the co-culture of passaged human chondrocytes and primary bovine chondrocytes. Journal of Histochemistry & Cytochemistry 60(8), pp. 576-587. (10.1369/0022155412449018)
- Parsons, P. et al. 2011. Type IX collagen interacts with fibronectin providing an important molecular bridge in articular cartilage. Journal of Biological Chemistry 286(40), pp. 34986-34997. (10.1074/jbc.M111.238188)
- Evans, L. C., Williams, A. S., Hayes, A. J., Jones, S. A. and Nowell, M. A. 2011. Suppression of leukocyte infiltration and cartilage degradation by selective inhibition of pre-B cell colony-enhancing factor/visfatin/nicotinamide phosphoribosyltransferase: Apo866-mediated therapy in human fibroblasts and murine collagen-induced arthritis. Arthritis and Rheumatism 63(7), pp. 1866-1877. (10.1002/art.30338)
- Evans, A., Williams, A. S., Hayes, A. J. and Nowell, M. A. 2011. Investigating the role of PBEF on cartilage integrity [Abstract]. International Journal of Experimental Pathology 92(3), pp. A33-A34. (10.1111/j.1365-2613.2010.00759.x)
- Akhtar, S., Bron, A., Hayes, A. J., Meek, K. M. A. and Caterson, B. 2011. Role of keratan sulphate (sulphated poly -N-acetyllactosamine repeats) in keratoconic cornea, histochemical, and ultrastructural analysis. Graefe's Archive for Clinical and Experimental Ophthalmology 249(3), pp. 413-420. (10.1007/s00417-010-1512-9)
- Hayes, A. J., Hughes, C. E., Ralphs, J. R. and Caterson, B. 2011. Chondroitin sulphate sulphation motif expression in the ontogeny of the intervertebral disc. European Cells and Materials 21, pp. 1-14.
- Silva, S., Henriques, M. C., Hayes, A. J., Oliveira, R., Azeredo, J. and Williams, D. W. 2011. Candida glabrata and Candida albicans co-infection of an in vitro oral epithelium. Journal of Oral Pathology and Medicine 40(5), pp. 421-427. (10.1111/j.1600-0714.2010.00981.x)
- Hayes, A. J. and Ralphs, J. R. 2011. The response of foetal annulus fibrosus cells to growth factors: modulation of matrix synthesis by TGF-β1 and IGF-1. Histochemistry and Cell Biology 136(2), pp. 163-175. (10.1007/s00418-011-0835-x)
- Thorp-Greenwood, F. L. et al. 2011. A 'Sleeping Trojan Horse' which transports metal ions into cells, localises in nucleoli, and has potential for bimodal fluorescence/PET imaging. Chemical Communications 47(11), pp. 3096-3098. (10.1039/c1cc10141b)
- Hayes, A. J., Isaacs, M. D., Hughes, C. E., Caterson, B. and Ralphs, J. R. 2011. Collagen fibrillogenesis in the development of the annulus fibrosus of the intervertebral disc. European Cells and Materials 22, pp. 226-241.
- Hayes, A. J., Lord, M. S., Smith, S. M., Smith, M. M., Whitelock, J. M., Weiss, A. S. and Melrose, J. 2011. Colocalization in vivo and association in vitro of perlecan and elastin. Histochemistry and Cell Biology 136(4), pp. 437-454. (10.1007/s00418-011-0854-7)
- Hayes, A. J., Smith, S. M., Gibson, M. A. and Melrose, J. 2011. Comparative immunolocalization of the elastin fiber-associated proteins fibrillin-1, LTBP-2, and MAGP-1 With components of the collagenous and proteoglycan matrix of the fetal human intervertebral disc. Spine 36(21), pp. E1365-E1372. (10.1097/BRS.0b013e31821fd23e)
- Coogan, M. P. et al. 2010. Probing intracellular oxygen by quenched phosphorescence lifetimes of nanoparticles containing polyacrylamide-embedded [Ru(dpp(SO3Na)2)3]Cl2. Photochemical and Photobiological Sciences 9(1), pp. 103-109. (10.1039/b9pp00071b)
- Fernández-Moreira, V. et al. 2010. Uptake and localisation of rhenium fac-tricarbonyl polypyridyls in fluorescent cell imaging experiments. Organic and Biomolecular Chemistry 8(17), pp. 3888-3901. (10.1039/c004610h)
- Ralphs, J. R., Caterson, B., Hughes, C. E. and Hayes, A. J. 2010. Chondroitin sulphate sulfation motifs in intervertebral disc development [Abstract]. European Cells and Materials 20(Supp 2), pp. 3.
- Gealy, E. C., Hayes, A. J., Buckwell, R., Young, R. D., Caterson, B., Quantock, A. J. and Ralphs, J. R. 2009. Actin and type I collagen propeptide distribution in the developing chick cornea. Investigative Ophthalmology & Visual Science 50(4), pp. 1653 -1658. (10.1167/iovs.08-2554)
- Nowell, M. A. et al. 2009. Therapeutic targeting of IL-6 trans signaling counteracts STAT3 control of experimental inflammatory arthritis. Journal of Immunology 182(1), pp. 613-622.
- Malic, S., Hill, K. E., Hayes, A. J., Percival, S. L., Thomas, D. W. and Williams, D. W. 2009. Detection and identification of specific bacteria in wound biofilms using peptide nucleic acid fluorescent in situ hybridization (PNA FISH). Microbiology-Sgm 155, pp. 2603-2611. (10.1099/mic.0.028712-0)
- Hayes, A. J., Hughes, C. E. and Caterson, B. 2008. Antibodies and immunohistochemistry in extracellular matrix research. Methods 45(1), pp. 10-21. (10.1016/j.ymeth.2008.01.011)
- Melrose, J., Hayes, A. J., Whitelock, J. M. and Little, C. B. 2008. Perlecan, the 'jack of all trades' proteoglycan of cartilaginous weight-bearing connective tissues. Bioessays 30(5), pp. 457-469. (10.1002/bies.20748)
- Amoroso, A. J. et al. 2008. 3-Chloromethylpyridyl bipyridine fac-tricarbonyl rhenium: a thiol-reactive luminophore for fluorescence microscopy accumulates in mitochondria. New Journal of Chemistry 32(7), pp. 1097-1102. (10.1039/b802215a)
- Hayes, A. J., Tudor, D., Nowell, M. A., Caterson, B. and Hughes, C. E. 2008. Chondroitin sulfate sulfation motifs as putative biomarkers for isolation of articular cartilage progenitor cells. Journal of Histochemistry & Cytochemistry 56(2), pp. 125-38. (10.1369/jhc.7A7320.2007)
- Akhtar, S., Kerr, B., Hayes, A. J., Hughes, C. E., Meek, K. M. and Caterson, B. 2008. Immunochemical localisation of keratan sulphate proteoglycans in cornea, sclera and limbus using a keratanase generated neoepitope monoclonal antibody. Investigative Ophthalmology and Visual Science 49(6), pp. 2424-2431. (10.1167/iovs.06-1498)
- Bazou, D., Coakley, W. T., Hayes, A. J. and Jackson, S. K. 2008. Long-term viability and proliferation of alginate-encapsulated 3-D HepG2 aggregates formed in an ultrasound trap. Toxicology in Vitro 22(5), pp. 1321-1331. (10.1016/j.tiv.2008.03.014)
- Caterson, B., Hayes, A. J., Tudor, D., Nowell, M. A. and Hughes, C. E. 2008. Chondroitin sulphate motifs as biomarkers for the stem/progenitor cell niche in musculoskeletal tissues. European Cells and Materials 16(Supp 2), pp. 47-47.
- Amoroso, A. J. et al. 2007. Rhenium fac tricarbonyl bisimine complexes: biologically useful fluorochromes for cell imaging applications. Chemical Communications(29), pp. 3066-3068. (10.1039/b706657k)
- Malic, S., Hill, K. E., Ralphs, J. R., Hayes, A. J., Thomas, D. W., Potts, A. J. C. and Williams, D. W. 2007. Characterisation of Candida albicans infection of an in vitro oral epithelial model using confocal laser scanning microscopy. Oral Microbiology And Immunolology 22(3), pp. 188-194. (10.1111/j.1399-302X.2007.00344.x)
- Hayes, A. J., Hall, A., Brown, L., Tubo, R. and Caterson, B. 2007. Macromolecular organization and in vitro growth characteristics of scaffold-free neocartilage grafts. Journal of Histochemistry & Cytochemistry 55(8), pp. 853-866. (10.1369/jhc.7A7210.2007)
- Gealy, E. C. et al. 2007. Differential expression of the keratan sulphate proteoglycan, keratocan, during chick corneal embryogenesis. Histochemistry and Cell Biology 128(6), pp. 551-555. (10.1007/s00418-007-0332-4)
- Aon, M. A., Cortassa, S., Lemar, K. M., Hayes, A. J. and Lloyd, D. 2007. Single and cell population respiratory oscillations in yeast: a 2-photon scanning laser microscopy study. FEBS Letters 581(1), pp. 8-14. (10.1016/j.febslet.2006.11.068)
- Blain, E. J., Gilbert, S. J., Hayes, A. J. and Duance, V. C. 2006. Disassembly of the vimentin cytoskeleton disrupts articular cartilage chondrocyte homeostasis.. Matrix biology 25(7), pp. 398-408. (10.1016/j.matbio.2006.06.002)
- Reed, K. R., Sansom, O. J., Hayes, A. J., Gescher, A. J., Peters, J. M. and Clarke, A. R. 2006. PPARδ status and mismatch repair mediated neoplasia in the mouse intestine. BMC Cancer 6(113) (10.1186/1471-2407-6-113)
- Hall, A. K., Hayes, A. J., Brown, L., Tubo, R. and Caterson, B. 2005. Tissue-engineered cartilage grafts for use in cartilage repair technologies [Abstract]. International Journal of Experimental Pathology 86(3), pp. A9. (10.1111/j.0959-9673.2005.00426.x)
- Young, R. D. et al. 2005. Atypical composition and ultrastructure of proteoglycans in the mouse corneal stroma. Investigative Ophthalmology and Visual Science 46(6), pp. 1973-1978. (10.1167/iovs.04-1309)
- Hayes, A. J., Tudor, D., Nowell, M. A., Hughes, C. E. and Caterson, B. 2005. Novel chondroitin sulphation motifs as putative biomarkers of articular cartilage chondroprogenitor cells [Abstract]. European Cells and Materials 10(Supp 2), pp. 72.
- Sansom, O. J. et al. 2004. Loss of Apc in vivo immediately perturbs Wnt signaling, differentiation, and migration. Genes and Development 18(12), pp. 1385-1390. (10.1101/gad.287404)
- Reed, K. R., Sansom, O. J., Hayes, A. J., Gescher, A. J., Winton, D. J., Peters, J. M. and Clarke, A. R. 2004. PPARδ status and Apc-mediated tumourigenesis in the mouse intestine. Oncogene 23(55), pp. 8992-8996. (10.1038/sj.onc.1208143)
- Hayes, A. J., Dowthwaite, G. P., Webster, S. V. and Archer, C. W. 2003. The distribution of Notch receptors and their ligands during articular cartilage development. Journal of Anatomy 202(6), pp. 495-502. (10.1046/j.1469-7580.2003.00185.x)
- Hayes, A. J., Benjamin, M. and Ralphs, J. R. 2001. Extracellular matrix in development of the intervertebral disc. Matrix Biology 20(2), pp. 107-121. (10.1016/S0945-053X(01)00125-1)
- Hayes, A., MacPherson, S., Morrison, H., Dowthwaite, G. and Archer, C. 2001. The development of articular cartilage: Evidence for an appositional growth mechanism. Anatomy and Embryology 203(6), pp. 469-479. (10.1007/s004290100178)
- Hayes, A. J., Benjamin, M. and Ralphs, J. R. 1999. Role of actin stress fibres in the development of the intervertebral disc: cytoskeletal control of extracellular matrix assembly. Developmental Dynamics 215(3), pp. 179-189. (10.1002/(SICI)1097-0177(199907)215:3<179::AID-AJA1>3.0.CO;2-Q)
- Williams, I. et al. 1999. Flow cytometry and other techniques show that Staphylococcus aureus undergoes significant physiological changes in the early stages of surface-attached culture. Microbiology 145(6), pp. 1325-1333. (10.1099/13500872-145-6-1325)
- Lloyd, D. et al. 1998. Micro-ecology of peat: minimally invasive analysis using confocal laser scanning microscopy, membrane inlet mass spectrometry and PCR amplification of methanogen-specific gene sequences. FEMS Microbiology Ecology 25(2), pp. 179-188. (10.1016/S0168-6496(97)00094-9)
- Shnyder, S. D., Hayes, A., Pringle, J. and Archer, C. 1998. P-glycoprotein and metallothionein expression and resistance to chemotherapy in osteosarcoma. British Journal of Cancer 78, pp. 757-759. (10.1038/bjc.1998.573)
- Biagini, G. A., Hayes, A. J., Suller, M. T. E., Winters, C., Finlay, B. J. and Archer, C. W. 1997. Hydrogenosomes of metopus contortus physiologically resemble mitochondria. Microbiology 143(5), pp. 1623-1629. (10.1099/00221287-143-5-1623)
- Hayes, A. J., Ralphs, J. R. and Benjamin, M. 1997. The involvement of actin in annulus formation in the rat lumbar intervertebral disc. Journal of Anatomy 191(1), pp. 125.
- Lloyd, D., Moran, C. A., Suller, M. T. E., Dinsdale, M. G. and Hayes, A. J. 1996. Flow cytometric monitoring of rhodamine 123 and a cyanine dye uptake by yeast during cider fermentation. Journal of the Institute of Brewing 102(4), pp. 251-259. (10.1002/j.2050-0416.1996.tb00910.x)
- Lloyd, D., Williams, A. G., Amann, R., Hayes, A. J., Durrant, L. and Ralphs, J. R. 1996. Intracellular prokaryotes in rumen ciliate protozoa: detection by confocal laser scanning microscopy after in situ hybridization with fluorescent 16S rRNA probes. European Journal of Protistology 32(4), pp. 523-531. (10.1016/S0932-4739(96)80011-3)
- Lloyd, D. and Hayes, A. J. 1995. Vigour, vitality and viability of microorganisms. FEMS Microbiology Letters 133(1-2), pp. 1-7. (10.1111/j.1574-6968.1995.tb07852.x)
Book sections
- Hayes, A. J. and Melrose, J. 2020. Keratan sulphate in the tumour environment. In: Tumor Microenvironment: Extracellular Matrix Components – Part A., Vol. 1245. Advances in Experimental Medicine and Biology Berlin: Springer-Verlag, pp. 39-66., (10.1007/978-3-030-40146-7_2)
- Lloyd, D., Hayes, A. J. and Ralphs, J. R. 1999. Confocal laser scanning microscopy of environmental samples. In: Edwards, C. ed. Environmental Monitoring of Bacteria., Vol. 12. USA: Springer, pp. 251-265., (10.1385/0-89603-566-2:251)
Conferences
- Jones, S., Caws, T., Hayes, A., Marsh Durban, V., Corteling, R. and Watson, P. 2019. Characterizing the cellular uptake of neural stem-cell derived exosomes using live-cell imaging techniques. Presented at: ISEV2019 Annual Meeting, Kyoto, Japan, 24-28 April 2019, Vol. 8. Vol. sup1. Wiley pp. IP12., (10.1080/20013078.2019.1593587)
Research
I have been research active for over thirty years and have published widely across the biomedical and life sciences, collaborating with researchers at local, national and international levels in diverse fields involving microscopy and bioimaging. Most of my published research involves the application of bioimaging to an understanding of the morphogenetic mechanisms underlying the ontogeny of musculoskeletal connective tissues.
Specifically, I am interested in the mechanisms by which connective tissue cells secrete and organise complex, highly ordered extracellular matrices and how these ordered matrices then influence cellular behaviour during subsequent tissue development, growth and repair. This theme was recently explored in two special issues of the International Journal of Molecular Sciences, 'Matrix embedded instructional cues direct tissue development and repair' which I guest-edited with a long-term collaborator at Sydney University, Dr Jim Melrose.
Most of my published work has focussed on two distinct connective tissues: the intervertebral disc and articular cartilage, both of which perform a shock absorbing role in the musculoskeletal system but do so in quite different ways.
What is the intervertebral disc and why is it important? Intervertebral discs are composite structures that occur between bony vertebrae of the spine - they have a deformable cartilaginous core, the nucleus pulposus, surrounded by a fibrous outer ring, the annulus fibrosus, that consists of a cross-ply organisation of oriented collagenous sheets (much like a car tyre). This lamellar organisation of oriented collagen allows twisting and bending movements of the spine whilst resisting compressive loads exerted by the nucleus pulposus. During aging, the disc is prone to prolapse ('slipped disc') and other degenerative changes which result in tissue destruction, pain and disability. Once the complex tissue organisation of the disc is destroyed it cannot be restored, often necessitating surgical intervention and removal of the damaged disc or spinal fusion. Understanding how the disc develops may offer insights for repair strategies. My research has focussed on a number of areas in intervertebral disc biology, including the (i) developmental mechanisms underlying the initial cellular orientation phase in the annulus fibrosus, in which a cellular template for oriented collagen deposition is first established, and the subsequent deposition and organisation of oriented extracellular matrix material around this cellular scaffold to form the functional layers of the annulus fibrosus, (ii) the reciprocal interactions that occur between cells and their surrounding extracellular matrix in the maintenance of homeostasis during later growth and maturation of the intervertebral disc, and (iii) how these interactions may be compromised following trauma (e.g. 'slipped disc') or with age-related degenerative changes, e.g. osteoarthritis.
What is articular cartilage and why is it important? Articular cartilage lines the articulating surface of bones in synovial joints. It functions to provide a smooth, low friction surface whilst resisting complex biomechanical loads experienced during gait. Like the intervertebral disc, articular cartilage is highly specialised to perform these functional roles. It is a highly stratified connective tissue that consists of distinct tissue zones, i.e., superficial, intermediate, deep, and calcified. Each tissue zone has characteristic differences in chondrocyte morphology, biochemical composition, and collagen fibre organisation. These structural differences endow unique biomechanical properties to the tissue as a whole, allowing it to respond appropriately to load. Despite this, articular cartilage is prone to age-related wear and tear with osteoarthritic changes progressing through the tissue strata over time and, ultimately, leading to destruction of the tissue necessitating joint replacement. Regneration of the articular cartilage tissue by stem cell therapy before this endpoint occurs is highly desirable. My research has shown that articular cartilage develops by a process of appositional growth during early joint formation. As articular chondrocytes progress through their differentiation pathway, regulated in part through Notch signalling, they give rise to each of the distinct tissue zones. This work led to the identification of a chondroprogenitor/articular cartilage stem cell with repair potential within the superficial zone, and the discovery of unique chondroitin sulphate motifs within the glycosaminoglycan chain structure of cell surface and pericellular proteoglycans within this stem cell niche. Mounting evidence indicates that these may be involved in regulating stem cell behaviour by modulating the activity of soluble signalling molecules, e.g., cytokines and growth factors, within the stem cell niche.
Understanding the morphogenetic mechansims underlying the development and growth of musculoskeletal connective tissues such as intervertebral disc and articular cartilage will continue to provide insights into potential repair strategies to offset degenerative joint disease. It is amazing to consider that these histologically distinct tissues have a similar range of extracellular matrix molecules, both soluble and structural, but it is how they are arranged and interact that endows the tissues with their unique functional properties.
Teaching
Over the last twenty years I have contributed to multiple teaching modules, practicals and workshops involving microscopy at the Bioimaging Research Hub and have been responsible for the supervision and training of undergraduates, PhD students, postgraduates and post-doctoral researchers in microscopy and bioimaging.
I have led on the development of several educational e-learning resources for student pedagogy, microscopy training/support and for science communication, outreach and engagement purposes. These include the following:
Virtual Microscopy Database for student teaching. This internally-facing database contains over 1000 high resolution digitised slide scanned image mosaics of histological/biological samples together with supporting metadata curated into four separate sub-collections, i.e., histopathology, parasitology, entomology and plant biology. All imaging, data curation, database design, programming and implementation was undertaken in-house at the Bioimaging Research Hub. The database is now widely used across multiple teaching modules by the Schools of Bioscience, Medicine and Dentistry. I am currently in the process of internationalising some of the content via the similarly-named, externally-facing Virtual Microscopy Database website maintained by the American Association of Anatomists.
The 3D Pollen Library Collection hosted via NIH3D. An educational resource containing over two hundred published, DOI-referenced models of pollen grains and spores for 3D printing and immersive learning (i.e. VR and AR) experiences.This is the largest of its kind in the world with global reach. You can learn more about the development of this resource via a recent Royal Microscopy Society (RMS) In Focus Magazine article (link here) and through the Bioimaging Hub News website (see below).
Standard Operating Procedures Repository (internal). A comprehensive, wiki-based, one-stop-shop for microscopy training and support. Includes SOPs, advanced methodology, YouTube training videos and user manuals for all hub imaging equipment, educational webinars for microscopy and imaging, risk assessments, experimental design, facility charges, grant applications and a lot more.
Bioimaging Hub News website. An informal source of news and technical information designed to make researchers aware of how our facilities and services can help their research and to highlight new equipment, research, innovation and collaborations involving the Bioimaging Research Hub.
Full details of all our e-learning and support resources are available via the Bioimaging Hub's Linktree page.
Biography
- 1989 - 1992: BSc (Hons) Zoology (Swansea University)
- 1992 - 1993: MSc Biological Electron Microscopy (Aberystwyth University)
- 1994 - 1996: Research Technician: Confocal microscopy facility(Cardiff University)
- 1996 - 1999: PhD Developmental Biology – Arthritis Research UK funded (Cardiff University). PhD Title: Development of the annulus fibrosus of the intervertebral disc.
- 1999 - 2005: Postdoctoral Research Assistant (Cardiff University)
- 2005 - 2016: Research Fellow; Facility Manager, Bioimaging Unit (Cardiff University)
- 2016 - present: Manager, Bioimaging Research Hub (Cardiff University).
Honours and awards
- New Investigator Recognition award for presenting the outstanding scientific paper at the 1997 Orthopaedic Research Society meeting, San Francisco, CA
Engagement
I make significant contributions to science communication, engagement and outreach at Cardiff University and have collaborated at local, national and international levels with both scientists and artists to inspire the next generation of scientists and promote public awareness of science.
You can read more about this work at the Bioimaging Hub News website (examples below) and through our social media channels (previously Twitter and now Linked In)
- IN FOCUS: The 3D Pollen Library: An Update.
- IN FOCUS: Breaking the Mould: Supersizing Pollen Grains as Gigantic Public Art Sculptures
- IN FOCUS: Plastic Fantastic - Making Pollen Models for the National Botanic Garden of Wales.
- IN FOCUS: Winging It In Paleobiology: Strange Tails from a Strange Time.
- IN FOCUS: 3D Pollen Prints Not To Be Sniffed At: Printing Pollen for the Met Office.
- IN FOCUS: Immersive Microscopy: 3D Visualisation and Manipulation of Microscopic Samples Through Virtual Realty.
- IN FOCUS: AR Palynology: Probing the Reality of Nature/Nature of Reality.
Contact Details
HayesAJ@cardiff.ac.uk
+44 29208 76611
Sir Martin Evans Building, Floor Ground Floor East, Room Cardiff School of Biosciences, The Sir Martin Evans Building (Room E/0.14A), Museum Avenue, Cardiff, CF10 3AX, Museum Avenue, Cardiff, CF10 3AX
+44 29208 76611
Sir Martin Evans Building, Floor Ground Floor East, Room Cardiff School of Biosciences, The Sir Martin Evans Building (Room E/0.14A), Museum Avenue, Cardiff, CF10 3AX, Museum Avenue, Cardiff, CF10 3AX
Research themes
Specialisms
- Bioimaging
- Microscopy
- Musculoskeletal Biology
- Connective Tissue Biology
- Extracellular Matrix Research
