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Professor Richard Stanton
Professor of Virology, School of Medicine
- Media commentator
- Available for postgraduate supervision
Overview
I am the head of Infection research within the Division of Infection & Immunity. I am a Professor within the 'Viral Immunology' group, and also the 'Cytomegalovirus and Adenovirus Virology' group, within the division of Infection & Immunity. My research covers the biology, immunology and diagnosis of human cytomegalovirus (HCMV) as well as the development of recombinant adenovirus vectors. More details can be seen at the Viral Immunology page, and the Cytomegalovirus and Adenovirus Virology page. We also work closely with the Oncolytic Adenovirus Virology group.
You can also find publication information on Google Scholar and Web of Science.
Publication
2024
- Samer, C. et al. 2024. Impaired endocytosis and accumulation in early endosomal compartments defines herpes simplex virus–mediated disruption of the nonclassical MHC class I–related molecule MR1. Journal of Biological Chemistry 300(10), article number: 107748. (10.1016/j.jbc.2024.107748)
- Obergfäll, D. et al. 2024. Cyclin-dependent kinase 8 represents a positive regulator of cytomegalovirus replication and a novel host target for antiviral strategies. Pharmaceutics 16(9), article number: 1238. (10.3390/pharmaceutics16091238)
- Bliss, C. M. et al. 2024. A pseudotyped adenovirus serotype 5 vector with serotype 49 fiber knob is an effective vector for vaccine and gene therapy applications. Molecular Therapy - Methods and Clinical Development 32(3), article number: 101308. (10.1016/j.omtm.2024.101308)
- Litvin, U., Wang, E. C., Stanton, R. J., Fielding, C. A. and Hughes, J. 2024. Evolution of the cytomegalovirus RL11 gene family in old world monkeys and great apes. Virus Evolution 10(1), article number: veae066. (10.1093/ve/veae066)
- Amratia, P. S., Kerr-Jones, L. E., Chapman, L., Marsden, M., Clement, M., Stanton, R. J. and Humphreys, I. R. 2024. Cytomegalovirus-induced peroxynitrite promotes virus entry and contributes to pathogenesis in a murine model of infection. mBio 15(8), article number: e03152-23. (10.1128/mbio.03152-23)
- Wesgate, R. et al. 2024. Impact of artificial accelerated ageing of PVC surfaces and surface degradation on disinfectant efficacy. Journal of Hospital Infection 149, pp. 1-13. (10.1016/j.jhin.2023.12.021)
- Finkel, Y. et al. 2024. A virally encoded high-resolution screen of cytomegalovirus dependencies. Nature 630, pp. 712-719. (10.1038/s41586-024-07503-z)
- Hunter, L. M. et al. 2024. HCMV US2 co-opts TRC8 to degrade the endoplasmic reticulum-resident protein LMAN2L. Journal of General Virology 105(4), article number: 1980. (10.1099/jgv.0.001980)
- Li, H. et al. 2024. Human cytomegalovirus degrades DMXL1 to inhibit autophagy, lysosomal acidification, and viral assembly. Cell Host & Microbe 32(4), pp. 466-478., article number: E11. (10.1016/j.chom.2024.02.013)
- Samer, C. et al. 2024. Multi-targeted loss of the antigen presentation molecule MR1 during HSV-1 and HSV-2 infection. iScience 27(2), article number: 108801. (10.1016/j.isci.2024.108801)
- Hulin-Curtis, S. et al. 2024. A targeted single mutation in influenza A virus universal epitope transforms immunogenicity and protective immunity via CD4+ T cell activation. Cell Reports 43(6), article number: 114259. (10.1016/j.celrep.2024.114259)
2023
- Chowdhury, S. et al. 2023. Inhibition of human cytomegalovirus replication by interferon alpha can involve multiple anti-viral factors. Journal of General Virology 104(12), article number: 1929. (10.1099/jgv.0.001929)
- Vlachava, V. et al. 2023. HCMV-secreted glycoprotein gpUL4 inhibits TRAIL-mediated apoptosis and NK cell activation. Proceedings of the National Academy of Sciences 120(49), article number: e2309077120. (10.1073/pnas.2309077120)
- Nan, X. et al. 2023. VarLOCK: sequencing-independent, rapid detection of SARS-CoV-2 variants of concern for point-of-care testing, qPCR pipelines and national wastewater surveillance. Scientific Reports 13(1), article number: 20832. (10.1038/s41598-023-47289-0)
- Duggan, K., Bentley, K., Stanton, R. J. and Maillard, J. 2023. Evaluating the antimicrobial efficacy of long-lasting hand sanitisers on skin. Journal of Hospital Infection (10.1016/j.jhin.2023.08.020)
- Rubina, A. et al. 2023. ADAM17 targeting by human cytomegalovirus remodels the cell surface proteome to simultaneously regulate multiple immune pathways. Proceedings of the National Academy of Sciences 120(33), article number: e2303155120. (10.1073/pnas.2303155120)
- Grant, M. D. et al. 2023. Combined anti-S1 and anti-S2 antibodies from hybrid immunity elicit potent cross-variant ADCC against SARS-CoV-2. JCI Insight 8(15), article number: e170681. (10.1172/jci.insight.170681)
- Semmes, E. C. et al. 2023. ADCC-activating antibodies correlate with decreased risk of congenital human cytomegalovirus transmission. JCI Insight 8(13), article number: e167768. (10.1172/jci.insight.167768)
- Meredith, R. T. et al. 2023. Differential cellular and humoral immune responses in immunocompromised individuals following multiple SARS-CoV-2 vaccinations. Frontiers in Cellular and Infection Microbiology 13 (10.3389/fcimb.2023.1207313)
- Lista, M. J. et al. 2023. Strain-dependent restriction of human cytomegalovirus by zinc finger antiviral proteins. Journal of Virology 97(3), article number: e01846-22. (10.1128/jvi.01846-22)
- Ashley, C. L. et al. 2023. Suppression of MR1 by human cytomegalovirus inhibits MAIT cell activation. Frontiers in Immunology 14 (10.3389/fimmu.2023.1107497)
2022
- Dangi, T. et al. 2022. Improved control of SARS-CoV-2 by treatment with nucleocapsid-specific monoclonal antibody. Journal of Clinical Investigation 132(23), article number: e162282. (10.1172/JCI162282)
- Saud, Z., Richards, C. A., Williams, G. and Stanton, R. J. 2022. Anti-viral organic coatings for high touch surfaces based on smart-release, Cu2+ containing pigments. Progress in Organic Coatings 172, article number: 107135. (10.1016/j.porgcoat.2022.107135)
- Scurr, M. J. et al. 2022. Magnitude of venous or capillary blood-derived SARS-CoV-2-specific T cell response determines COVID-19 immunity. Nature Communications 13, article number: 5422. (10.1038/s41467-022-32985-8)
- Statkute, E., Wang, E. and Stanton, R. 2022. An optimised CRISPR/Cas9 adenovirus vector (AdZ-CRISPR) for high throughput cloning of sgRNA, using enhanced sgRNA and Cas9 variants. Human Gene Therapy 33(17-18), pp. 990-1001. (10.1089/hum.2021.120)
- Crozier, T. W. et al. 2022. Quantitative proteomic analysis of SARS-CoV-2 infection of primary human airway ciliated cells and lung epithelial cells demonstrates the effectiveness of SARS-CoV-2 innate immune evasion. Wellcome Open Research 7, article number: 224. (10.12688/wellcomeopenres.17946.1)
- Clement, M. et al. 2022. IFITM3 restricts virus-induced inflammatory cytokine production by limiting Nogo-B mediated TLR responses. Nature Communications 13(1), article number: 5294. (10.1038/s41467-022-32587-4)
- Weiler, N., Sampaio, K. L., Stanton, R. J. and Sinzger, C. 2022. Combined knockdown of RL13 and UL128 for release of cell-free infectivity from recent HCMV isolates. Journal of Virological Methods 305, article number: 114537. (10.1016/j.jviromet.2022.114537)
- Saud, Z. et al. 2022. Mechanically ventilated patients shed high titre live SARS-CoV2 for extended periods from both the upper and lower respiratory tract. Clinical Infectious Diseases 75(1), pp. e82-e88., article number: ciac170. (10.1093/cid/ciac170)
- Saud, Z. et al. 2022. The SARS-CoV2 envelope differs from host cells, exposes pro-coagulant lipids, and is disrupted in vivo by oral rinses. Journal of Lipid Research 63(6), article number: 100208. (10.1016/j.jlr.2022.100208)
- Fielding, C. A. et al. 2022. SARS-CoV-2 host-shutoff impacts innate NK cell functions, but antibody-dependent NK activity is strongly activated through non-spike antibodies. eLife 11 (10.7554/eLife.74489)
- Tizaoui, C. et al. 2022. Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media. Journal of Hazardous Materials 428, article number: 128251. (10.1016/j.jhazmat.2022.128251)
- Wang, E. C., Fielding, C. A. and Stanton, R. J. 2022. Concerns over functional experiments, interpretation, and required controls. Journal of Clinical Investigation 132(5), article number: e157369. (10.1172/JCI157369)
- Nightingale, K. et al. 2022. Human cytomegalovirus protein RL1 degrades the antiviral factor SLFN11 via recruitment of the CRL4 E3 ubiquitin ligase complex. Proceedings of the National Academy of Sciences 119(6), article number: e2108173119. (10.1073/pnas.2108173119)
- Nan, X. et al. 2022. VarLOCK - sequencing independent, rapid detection of SARS-CoV-2 variants of concern for point-of-care testing, qPCR pipelines and national wastewater surveillance. [Online]. medRxix: (10.1101/2022.01.06.21268555) Available at: https://www.medrxiv.org/content/10.1101/2022.01.06.21268555v1
2021
- Bentley, K. and Stanton, R. J. 2021. Hydroxypropyl methylcellulose-based nasal sprays effectively inhibit in vitro SARS-CoV-2 infection and spread. Viruses 13(12), article number: 2345. (10.3390/v13122345)
- Hyde, K. et al. 2021. Limited replication of human cytomegalovirus in a trophoblast cell line. Journal of General Virology 102(11), article number: 1683. (10.1099/jgv.0.001683)
- Lau, B. et al. 2021. Human cytomegalovirus RNA2.7 is required for upregulating multiple cellular genes to promote cell motility and viral spread late in lytic infection. Journal of Virology 95(20), article number: e00698-21. (10.1128/JVI.00698-21)
- Wickenhagen, A. et al. 2021. A prenylated dsRNA sensor protects against severe COVID-19. Science 374(6567), pp. 535-536. (10.1126/science.abj3624)
- Braun, B., Fischer, D., Laib Sampaio, K., Mezger, M., Stöhr, D., Stanton, R. J. and Sinzger, C. 2021. Peptide derivatives of platelet-derived growth factor receptor alpha inhibit cell-associated spread of human cytomegalovirus. Viruses 13(9), article number: 1780. (10.3390/v13091780)
- Clement, M. et al. 2021. IFITM3 regulates virus-induced inflammatory cytokine production by titrating Nogo-B orchestration of TLR responses. [Online]. bioRxiv. Available at: https://doi.org/10.1101/2021.07.23.453513
- Weiler, N. et al. 2021. Role of envelope glycoprotein complexes in cell-associated spread of human cytomegalovirus. Viruses 13(4), article number: 614. (10.3390/v13040614)
- Vlachava, V. et al. 2021. Monoclonal antibodies targeting nonstructural viral antigens can activate ADCC against human cytomegalovirus. Journal of Clinical Investigation 131(4), article number: e139296. (10.1172/JCI139296)
2020
- Fletcher-Etherington, A. et al. 2020. Human cytomegalovirus protein pUL36: a dual cell death pathway inhibitor. Proceedings of the National Academy of Sciences 117(31), pp. 18771 - 18779. (10.1073/pnas.2001887117)
- Lau, B. et al. 2020. Human Cytomegalovirus long non-coding RNA1.2 suppresses extracellular release of the pro-inflammatory Cytokine IL-6 by blocking NF-κB activation. Frontiers in Cellular and Infection Microbiology 10, article number: 361. (10.3389/fcimb.2020.00361)
- Houldcroft, C. J. et al. 2020. Assessing anti-HCMV cell mediated immune responses in transplant recipients and healthy controls using a novel functional assay. Frontiers in Cellular and Infection Microbiology 10, article number: 275. (10.3389/fcimb.2020.00275)
- O'Donnell, V. B. et al. 2020. Potential role of oral rinses targeting the viral lipid envelope in SARS-CoV-2 infection. Function 1(1), article number: zqaa002. (10.1093/function/zqaa002)
- Scurr, M. J. et al. 2020. Cancer antigen discovery is enabled by RNA-sequencing of highly purified malignant and non-malignant cells. Clinical Cancer Research (10.1158/1078-0432.CCR-19-3087)
- Elasifer, H. et al. 2020. Downregulation of HLA-I by the molluscum contagiosum virus mc080 impacts NK-cell recognition and promotes CD8+ T-cell evasion. Journal of General Virology 101, pp. 863-872. (10.1099/jgv.0.001417)
- Taher, H. et al. 2020. In vitro and in vivo characterization of a recombinant rhesus cytomegalovirus containing a complete genome. PLoS Pathogens 16(11), article number: e1008666. (10.1371/journal.ppat.1008666)
2019
- Nobre, L. V. et al. 2019. Human cytomegalovirus interactome analysis identifies degradation hubs, domain associations and viral protein functions. eLife 8, article number: e49894. (10.7554/eLife.49894)
2018
- Patel, M., Vlachava, V., Forbes, S. K., Fielding, C. A., Stanton, R. J. and Wang, E. 2018. HCMV-encoded NK modulators: Lessons from in vitro and in vivo genetic variation. Frontiers in Immunology 9, article number: 2214. (10.3389/fimmu.2018.02214)
- Falk, J. et al. 2018. Large-scale screening of HCMV-seropositive blood donors indicates that HCMV effectively escapes from antibodies by cell-associated spread. Viruses 10(9), article number: 500. (10.3390/v10090500)
- Nightingale, K. et al. 2018. High-definition analysis of host protein stability during human cytomegalovirus infection reveals antiviral factors and viral evasion mechanisms. Cell Host and Microbe 24(3), pp. 447-460.e11. (10.1016/j.chom.2018.07.011)
- Zhang, L., Zhou, M., Stanton, R., Kamil, J. and Ryckman, B. J. 2018. Expression levels of glycoprotein O (gO) vary between strains of human cytomegalovirus, influencing the assembly of gH/gL complexes and virion infectivity. Journal of Virology 92(15), article number: e00606-18. (10.1128/JVI.00606-18)
- Wang, E. C. Y. et al. 2018. Suppression of costimulation by human cytomegalovirus promotes evasion of cellular immune defenses. Proceedings of the National Academy of Sciences 115(19), pp. 4998-5003. (10.1073/pnas.1720950115)
2017
- Murrell, I. et al. 2017. The pentameric complex drives immunologically covert cell -cell transmission of wild-type human cytomegalovirus. Proceedings of the National Academy of Sciences 114(23), pp. 6104-6109. (10.1073/pnas.1704809114)
- Fielding, C. A. et al. 2017. Control of immune ligands by members of a cytomegalovirus gene expansion suppresses natural killer cell activation. eLife 6, article number: e22206. (10.7554/eLife.22206)
2016
- Assaifan, A. K., Lloyd, J. S., Samavat, S., Deganello, D., Stanton, R. J. and Teng, K. S. 2016. Nanotextured surface on flexographic printed ZnO thin films for low-cost non-Faradaic biosensors. ACS Applied Materials & Interfaces 8(49), pp. 33802-33810. (10.1021/acsami.6b11640)
- Laib Sampaio, K., Stegmann, C., Brizic, I., Adler, B., Stanton, R. J. and Sinzger, C. 2016. The contribution of pUL74 to growth of human cytomegalovirus is masked in the presence of RL13 and UL128 expression. Journal of General Virology 97, pp. 1917-1927. (10.1099/jgv.0.000475)
- Murrell, I. et al. 2016. Genetic stability of BAC-deerived human cytomegalovirus during culture in vitro. Journal of Virology 90(8), pp. 3929-3943. (10.1128/JVI.02858-15)
- Karniely, S. et al. 2016. Human cytomegalovirus infection upregulates the mitochondrial transcription and translation machineries. mBio 7(2), article number: e00029-16. (10.1128/mBio.00029-16)
- Chen, K. C., Stanton, R. J., Banat, J. J. and Wills, M. R. 2016. Leukocyte Immunoglobulin-Like Receptor 1- expressing human natural killer cell subsets differentially recognize isolates of human cytomegalovirus through the viral MHC Class I homolog UL18. Journal of Virology 90(6), pp. 3123-3137. (10.1128/JVI.02614-15)
2015
- Wilkinson, G. W. G. et al. 2015. Human cytomegalovirus: taking the strain. Medical Microbiology and Immunology 204(3), pp. 273-284. (10.1007/s00430-015-0411-4)
- Uusi-Kerttula, H. et al. 2015. Incorporation of peptides targeting EGFR and FGFR1 into the adenoviral fibre knob domain, and their evaluation as targeted cancer therapies. Human Gene Therapy 26(5), pp. 320-329. (10.1089/hum.2015.015)
- Hsu, J. et al. 2015. Plasma membrane profiling defines an expanded class of cell surface proteins selectively targeted for degradation by HCMV US2 in cooperation with UL141. PLoS Pathogens 11(4), article number: e1004811. (10.1371/journal.ppat.1004811)
- Stack, G. et al. 2015. CD200 receptor restriction of myeloid cell responses antagonizes antiviral immunity and facilitates cytomegalovirus persistence within mucosal tissue. PLoS Pathogens 11(2), article number: e1004641. (10.1371/journal.ppat.1004641)
2014
- Gabaev, I. et al. 2014. Expression of the human cytomegalovirus UL11 glycoprotein in viral infection and evaluation of its effect on virus-specific CD8 T cells. Journal of Virology 88(24), pp. 14326-14339. (10.1128/JVI.01691-14)
- Stanton, R. J. et al. 2014. HCMV pUL135 remodels the actin cytoskeleton to impair immune recognition of infected cells. Cell Host and Microbe 16(2), pp. 201-214. (10.1016/j.chom.2014.07.005)
- Weekes, M. et al. 2014. Quantitative temporal viromics: an approach to investigate host-pathogen interaction. Cell 157(6), pp. 1460-1472. (10.1016/j.cell.2014.04.028)
- Fielding, C. A. et al. 2014. Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation. PLoS Pathogens 10(5), article number: e1004058. (10.1371/journal.ppat.1004058)
2013
- Aicheler, R., Wang, E., Tomasec, P., Wilkinson, G. W. G. and Stanton, R. J. 2013. Potential for natural killer cell-mediated antibody-dependent cellular cytotoxicity for control of human cytomegalovirus. Antibodies 2(4), pp. 617-635. (10.3390/antib2040617)
- Murrell, I., Tomasec, P., Wilkie, G. S., Dargan, D. J., Davison, A. J. and Stanton, R. J. 2013. Impact of sequence variation in the UL128 locus on production of human cytomegalovirus in fibroblast and epithelial cells. Journal of Virology 87(19), pp. 10489-10500. (10.1128/JVI.01546-13)
- Aicheler, R. J. and Stanton, R. J. 2013. Functional NK cell cytotoxicity assays against virus infected cells. In: Bailer, S. M. and Lieber, D. eds. Virus-Host Interactions., Vol. 1064. Methods in Molecular Biology Springer, pp. 275-287., (10.1007/978-1-62703-601-6_20)
- Smith, W. et al. 2013. Human cytomegalovirus glycoprotein UL141 targets the TRAIL death receptors to thwart host innate antiviral defenses. Cell Host & Microbe 13(3), pp. 324-335. (10.1016/j.chom.2013.02.003)
2012
- Fielding, C. A. et al. 2012. A novel human cytomegalovirus gene function targets the natural killer cell activating ligand MICA for lysosomal degradation [Abstract]. Immunology 137(S1), pp. 154. (10.1111/imm.12001)
- Prod'homme, V. et al. 2012. Human cytomegalovirus UL40 signal peptide regulates cell surface expression of the NK cell ligands HLA-E and gpUL18. The Journal of Immunology 188(6), pp. 2794-2804. (10.4049/jimmunol.1102068)
2011
- Gabaev, I. et al. 2011. The human cytomegalovirus ul11 protein interacts with the receptor tyrosine phosphatase cd45, resulting in functional paralysis of t cells. PLoS Pathogens 7(12), article number: e1002432. (10.1371/journal.ppat.1002432)
2010
- Stanton, R. J. et al. 2010. Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication. Journal of Clinical Investigation 120(9), pp. 3191-3208. (10.1172/JCI42955)
- Prod'homme, V. et al. 2010. Human cytomegalovirus UL141 promotes efficient downregulation of the natural killer cell activating ligand CD112. Journal of General Virology 91(8), pp. 2034-2039. (10.1099/vir.0.021931-0)
- Dargan, D. J. et al. 2010. Sequential mutations associated with adaptation of human cytomegalovirus to growth in cell culture. Journal of General Virology 91(6), pp. 1535-1546. (10.1099/vir.0.018994-0)
2008
- Stanton, R. J., McSharry, B. P., Armstrong, M. L., Tomasec, P. and Wilkinson, G. W. G. 2008. Re-engineering adenovirus vector systems to enable high-throughput analyses of gene function. Biotechniques 45(6), pp. 659-668.
- McSharry, B. P. et al. 2008. Adenovirus E3/19K promotes evasion of NK cell recognition by intracellular sequestration of the NKG2D ligands major histocompatibility complex class I chain-related proteins A and B. Journal of Virology 82(9), pp. 4585-4594. (10.1128/JVI.02251-07)
- Wilkinson, G. W. G. et al. 2008. Modulation of natural killer cells by human cytomegalovirus. Journal of Clinical Virology 41(3), pp. 206-212. (10.1016/j.jcv.2007.10.027)
2007
- Stanton, R. J., McSharry, B. P., Rickards, C. R., Wang, E. C. Y., Tomasec, P. and Wilkinson, G. W. G. 2007. Cytomegalovirus destruction of focal adhesions revealed in a high-throughput Western blot analysis of cellular protein expression. Journal of Virology 81(15), pp. 7860-7872. (10.1128/JVI.02247-06)
- Prod'homme, V. et al. 2007. The Human Cytomegalovirus MHC Class I Homolog UL18 Inhibits LIR-1+ but Activates LIR-1- NK Cells. The Journal of Immunology 178(7), pp. 4473-4481.
- Tomasec, P. et al. 2007. Adenovirus vector delivery stimulates natural killer cell recognition. Journal of General Virology 88(4), pp. 1103-1108. (10.1099/vir.0.82685-0)
2005
- Stanton, R. J., Westmoreland, D., Fox, J. D., Davison, A. J. and Wilkinson, G. W. G. 2005. Stability of human cytomegalovirus genotypes in persistently infected renal transplant recipients. Journal of Medical Virology 75(1), pp. 42-46. (10.1002/jmv.20235)
2003
- Stanton, R. J., Wilkinson, G. W. G. and Fox, J. D. 2003. Analysis of human herpesvirus-6 IE1 sequence variation in clinical samples. Journal of Medical Virology 71(4), pp. 578-584. (10.1002/jmv.10508)
Articles
- Samer, C. et al. 2024. Impaired endocytosis and accumulation in early endosomal compartments defines herpes simplex virus–mediated disruption of the nonclassical MHC class I–related molecule MR1. Journal of Biological Chemistry 300(10), article number: 107748. (10.1016/j.jbc.2024.107748)
- Obergfäll, D. et al. 2024. Cyclin-dependent kinase 8 represents a positive regulator of cytomegalovirus replication and a novel host target for antiviral strategies. Pharmaceutics 16(9), article number: 1238. (10.3390/pharmaceutics16091238)
- Bliss, C. M. et al. 2024. A pseudotyped adenovirus serotype 5 vector with serotype 49 fiber knob is an effective vector for vaccine and gene therapy applications. Molecular Therapy - Methods and Clinical Development 32(3), article number: 101308. (10.1016/j.omtm.2024.101308)
- Litvin, U., Wang, E. C., Stanton, R. J., Fielding, C. A. and Hughes, J. 2024. Evolution of the cytomegalovirus RL11 gene family in old world monkeys and great apes. Virus Evolution 10(1), article number: veae066. (10.1093/ve/veae066)
- Amratia, P. S., Kerr-Jones, L. E., Chapman, L., Marsden, M., Clement, M., Stanton, R. J. and Humphreys, I. R. 2024. Cytomegalovirus-induced peroxynitrite promotes virus entry and contributes to pathogenesis in a murine model of infection. mBio 15(8), article number: e03152-23. (10.1128/mbio.03152-23)
- Wesgate, R. et al. 2024. Impact of artificial accelerated ageing of PVC surfaces and surface degradation on disinfectant efficacy. Journal of Hospital Infection 149, pp. 1-13. (10.1016/j.jhin.2023.12.021)
- Finkel, Y. et al. 2024. A virally encoded high-resolution screen of cytomegalovirus dependencies. Nature 630, pp. 712-719. (10.1038/s41586-024-07503-z)
- Hunter, L. M. et al. 2024. HCMV US2 co-opts TRC8 to degrade the endoplasmic reticulum-resident protein LMAN2L. Journal of General Virology 105(4), article number: 1980. (10.1099/jgv.0.001980)
- Li, H. et al. 2024. Human cytomegalovirus degrades DMXL1 to inhibit autophagy, lysosomal acidification, and viral assembly. Cell Host & Microbe 32(4), pp. 466-478., article number: E11. (10.1016/j.chom.2024.02.013)
- Samer, C. et al. 2024. Multi-targeted loss of the antigen presentation molecule MR1 during HSV-1 and HSV-2 infection. iScience 27(2), article number: 108801. (10.1016/j.isci.2024.108801)
- Hulin-Curtis, S. et al. 2024. A targeted single mutation in influenza A virus universal epitope transforms immunogenicity and protective immunity via CD4+ T cell activation. Cell Reports 43(6), article number: 114259. (10.1016/j.celrep.2024.114259)
- Chowdhury, S. et al. 2023. Inhibition of human cytomegalovirus replication by interferon alpha can involve multiple anti-viral factors. Journal of General Virology 104(12), article number: 1929. (10.1099/jgv.0.001929)
- Vlachava, V. et al. 2023. HCMV-secreted glycoprotein gpUL4 inhibits TRAIL-mediated apoptosis and NK cell activation. Proceedings of the National Academy of Sciences 120(49), article number: e2309077120. (10.1073/pnas.2309077120)
- Nan, X. et al. 2023. VarLOCK: sequencing-independent, rapid detection of SARS-CoV-2 variants of concern for point-of-care testing, qPCR pipelines and national wastewater surveillance. Scientific Reports 13(1), article number: 20832. (10.1038/s41598-023-47289-0)
- Duggan, K., Bentley, K., Stanton, R. J. and Maillard, J. 2023. Evaluating the antimicrobial efficacy of long-lasting hand sanitisers on skin. Journal of Hospital Infection (10.1016/j.jhin.2023.08.020)
- Rubina, A. et al. 2023. ADAM17 targeting by human cytomegalovirus remodels the cell surface proteome to simultaneously regulate multiple immune pathways. Proceedings of the National Academy of Sciences 120(33), article number: e2303155120. (10.1073/pnas.2303155120)
- Grant, M. D. et al. 2023. Combined anti-S1 and anti-S2 antibodies from hybrid immunity elicit potent cross-variant ADCC against SARS-CoV-2. JCI Insight 8(15), article number: e170681. (10.1172/jci.insight.170681)
- Semmes, E. C. et al. 2023. ADCC-activating antibodies correlate with decreased risk of congenital human cytomegalovirus transmission. JCI Insight 8(13), article number: e167768. (10.1172/jci.insight.167768)
- Meredith, R. T. et al. 2023. Differential cellular and humoral immune responses in immunocompromised individuals following multiple SARS-CoV-2 vaccinations. Frontiers in Cellular and Infection Microbiology 13 (10.3389/fcimb.2023.1207313)
- Lista, M. J. et al. 2023. Strain-dependent restriction of human cytomegalovirus by zinc finger antiviral proteins. Journal of Virology 97(3), article number: e01846-22. (10.1128/jvi.01846-22)
- Ashley, C. L. et al. 2023. Suppression of MR1 by human cytomegalovirus inhibits MAIT cell activation. Frontiers in Immunology 14 (10.3389/fimmu.2023.1107497)
- Dangi, T. et al. 2022. Improved control of SARS-CoV-2 by treatment with nucleocapsid-specific monoclonal antibody. Journal of Clinical Investigation 132(23), article number: e162282. (10.1172/JCI162282)
- Saud, Z., Richards, C. A., Williams, G. and Stanton, R. J. 2022. Anti-viral organic coatings for high touch surfaces based on smart-release, Cu2+ containing pigments. Progress in Organic Coatings 172, article number: 107135. (10.1016/j.porgcoat.2022.107135)
- Scurr, M. J. et al. 2022. Magnitude of venous or capillary blood-derived SARS-CoV-2-specific T cell response determines COVID-19 immunity. Nature Communications 13, article number: 5422. (10.1038/s41467-022-32985-8)
- Statkute, E., Wang, E. and Stanton, R. 2022. An optimised CRISPR/Cas9 adenovirus vector (AdZ-CRISPR) for high throughput cloning of sgRNA, using enhanced sgRNA and Cas9 variants. Human Gene Therapy 33(17-18), pp. 990-1001. (10.1089/hum.2021.120)
- Crozier, T. W. et al. 2022. Quantitative proteomic analysis of SARS-CoV-2 infection of primary human airway ciliated cells and lung epithelial cells demonstrates the effectiveness of SARS-CoV-2 innate immune evasion. Wellcome Open Research 7, article number: 224. (10.12688/wellcomeopenres.17946.1)
- Clement, M. et al. 2022. IFITM3 restricts virus-induced inflammatory cytokine production by limiting Nogo-B mediated TLR responses. Nature Communications 13(1), article number: 5294. (10.1038/s41467-022-32587-4)
- Weiler, N., Sampaio, K. L., Stanton, R. J. and Sinzger, C. 2022. Combined knockdown of RL13 and UL128 for release of cell-free infectivity from recent HCMV isolates. Journal of Virological Methods 305, article number: 114537. (10.1016/j.jviromet.2022.114537)
- Saud, Z. et al. 2022. Mechanically ventilated patients shed high titre live SARS-CoV2 for extended periods from both the upper and lower respiratory tract. Clinical Infectious Diseases 75(1), pp. e82-e88., article number: ciac170. (10.1093/cid/ciac170)
- Saud, Z. et al. 2022. The SARS-CoV2 envelope differs from host cells, exposes pro-coagulant lipids, and is disrupted in vivo by oral rinses. Journal of Lipid Research 63(6), article number: 100208. (10.1016/j.jlr.2022.100208)
- Fielding, C. A. et al. 2022. SARS-CoV-2 host-shutoff impacts innate NK cell functions, but antibody-dependent NK activity is strongly activated through non-spike antibodies. eLife 11 (10.7554/eLife.74489)
- Tizaoui, C. et al. 2022. Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media. Journal of Hazardous Materials 428, article number: 128251. (10.1016/j.jhazmat.2022.128251)
- Wang, E. C., Fielding, C. A. and Stanton, R. J. 2022. Concerns over functional experiments, interpretation, and required controls. Journal of Clinical Investigation 132(5), article number: e157369. (10.1172/JCI157369)
- Nightingale, K. et al. 2022. Human cytomegalovirus protein RL1 degrades the antiviral factor SLFN11 via recruitment of the CRL4 E3 ubiquitin ligase complex. Proceedings of the National Academy of Sciences 119(6), article number: e2108173119. (10.1073/pnas.2108173119)
- Bentley, K. and Stanton, R. J. 2021. Hydroxypropyl methylcellulose-based nasal sprays effectively inhibit in vitro SARS-CoV-2 infection and spread. Viruses 13(12), article number: 2345. (10.3390/v13122345)
- Hyde, K. et al. 2021. Limited replication of human cytomegalovirus in a trophoblast cell line. Journal of General Virology 102(11), article number: 1683. (10.1099/jgv.0.001683)
- Lau, B. et al. 2021. Human cytomegalovirus RNA2.7 is required for upregulating multiple cellular genes to promote cell motility and viral spread late in lytic infection. Journal of Virology 95(20), article number: e00698-21. (10.1128/JVI.00698-21)
- Wickenhagen, A. et al. 2021. A prenylated dsRNA sensor protects against severe COVID-19. Science 374(6567), pp. 535-536. (10.1126/science.abj3624)
- Braun, B., Fischer, D., Laib Sampaio, K., Mezger, M., Stöhr, D., Stanton, R. J. and Sinzger, C. 2021. Peptide derivatives of platelet-derived growth factor receptor alpha inhibit cell-associated spread of human cytomegalovirus. Viruses 13(9), article number: 1780. (10.3390/v13091780)
- Weiler, N. et al. 2021. Role of envelope glycoprotein complexes in cell-associated spread of human cytomegalovirus. Viruses 13(4), article number: 614. (10.3390/v13040614)
- Vlachava, V. et al. 2021. Monoclonal antibodies targeting nonstructural viral antigens can activate ADCC against human cytomegalovirus. Journal of Clinical Investigation 131(4), article number: e139296. (10.1172/JCI139296)
- Fletcher-Etherington, A. et al. 2020. Human cytomegalovirus protein pUL36: a dual cell death pathway inhibitor. Proceedings of the National Academy of Sciences 117(31), pp. 18771 - 18779. (10.1073/pnas.2001887117)
- Lau, B. et al. 2020. Human Cytomegalovirus long non-coding RNA1.2 suppresses extracellular release of the pro-inflammatory Cytokine IL-6 by blocking NF-κB activation. Frontiers in Cellular and Infection Microbiology 10, article number: 361. (10.3389/fcimb.2020.00361)
- Houldcroft, C. J. et al. 2020. Assessing anti-HCMV cell mediated immune responses in transplant recipients and healthy controls using a novel functional assay. Frontiers in Cellular and Infection Microbiology 10, article number: 275. (10.3389/fcimb.2020.00275)
- O'Donnell, V. B. et al. 2020. Potential role of oral rinses targeting the viral lipid envelope in SARS-CoV-2 infection. Function 1(1), article number: zqaa002. (10.1093/function/zqaa002)
- Scurr, M. J. et al. 2020. Cancer antigen discovery is enabled by RNA-sequencing of highly purified malignant and non-malignant cells. Clinical Cancer Research (10.1158/1078-0432.CCR-19-3087)
- Elasifer, H. et al. 2020. Downregulation of HLA-I by the molluscum contagiosum virus mc080 impacts NK-cell recognition and promotes CD8+ T-cell evasion. Journal of General Virology 101, pp. 863-872. (10.1099/jgv.0.001417)
- Taher, H. et al. 2020. In vitro and in vivo characterization of a recombinant rhesus cytomegalovirus containing a complete genome. PLoS Pathogens 16(11), article number: e1008666. (10.1371/journal.ppat.1008666)
- Nobre, L. V. et al. 2019. Human cytomegalovirus interactome analysis identifies degradation hubs, domain associations and viral protein functions. eLife 8, article number: e49894. (10.7554/eLife.49894)
- Patel, M., Vlachava, V., Forbes, S. K., Fielding, C. A., Stanton, R. J. and Wang, E. 2018. HCMV-encoded NK modulators: Lessons from in vitro and in vivo genetic variation. Frontiers in Immunology 9, article number: 2214. (10.3389/fimmu.2018.02214)
- Falk, J. et al. 2018. Large-scale screening of HCMV-seropositive blood donors indicates that HCMV effectively escapes from antibodies by cell-associated spread. Viruses 10(9), article number: 500. (10.3390/v10090500)
- Nightingale, K. et al. 2018. High-definition analysis of host protein stability during human cytomegalovirus infection reveals antiviral factors and viral evasion mechanisms. Cell Host and Microbe 24(3), pp. 447-460.e11. (10.1016/j.chom.2018.07.011)
- Zhang, L., Zhou, M., Stanton, R., Kamil, J. and Ryckman, B. J. 2018. Expression levels of glycoprotein O (gO) vary between strains of human cytomegalovirus, influencing the assembly of gH/gL complexes and virion infectivity. Journal of Virology 92(15), article number: e00606-18. (10.1128/JVI.00606-18)
- Wang, E. C. Y. et al. 2018. Suppression of costimulation by human cytomegalovirus promotes evasion of cellular immune defenses. Proceedings of the National Academy of Sciences 115(19), pp. 4998-5003. (10.1073/pnas.1720950115)
- Murrell, I. et al. 2017. The pentameric complex drives immunologically covert cell -cell transmission of wild-type human cytomegalovirus. Proceedings of the National Academy of Sciences 114(23), pp. 6104-6109. (10.1073/pnas.1704809114)
- Fielding, C. A. et al. 2017. Control of immune ligands by members of a cytomegalovirus gene expansion suppresses natural killer cell activation. eLife 6, article number: e22206. (10.7554/eLife.22206)
- Assaifan, A. K., Lloyd, J. S., Samavat, S., Deganello, D., Stanton, R. J. and Teng, K. S. 2016. Nanotextured surface on flexographic printed ZnO thin films for low-cost non-Faradaic biosensors. ACS Applied Materials & Interfaces 8(49), pp. 33802-33810. (10.1021/acsami.6b11640)
- Laib Sampaio, K., Stegmann, C., Brizic, I., Adler, B., Stanton, R. J. and Sinzger, C. 2016. The contribution of pUL74 to growth of human cytomegalovirus is masked in the presence of RL13 and UL128 expression. Journal of General Virology 97, pp. 1917-1927. (10.1099/jgv.0.000475)
- Murrell, I. et al. 2016. Genetic stability of BAC-deerived human cytomegalovirus during culture in vitro. Journal of Virology 90(8), pp. 3929-3943. (10.1128/JVI.02858-15)
- Karniely, S. et al. 2016. Human cytomegalovirus infection upregulates the mitochondrial transcription and translation machineries. mBio 7(2), article number: e00029-16. (10.1128/mBio.00029-16)
- Chen, K. C., Stanton, R. J., Banat, J. J. and Wills, M. R. 2016. Leukocyte Immunoglobulin-Like Receptor 1- expressing human natural killer cell subsets differentially recognize isolates of human cytomegalovirus through the viral MHC Class I homolog UL18. Journal of Virology 90(6), pp. 3123-3137. (10.1128/JVI.02614-15)
- Wilkinson, G. W. G. et al. 2015. Human cytomegalovirus: taking the strain. Medical Microbiology and Immunology 204(3), pp. 273-284. (10.1007/s00430-015-0411-4)
- Uusi-Kerttula, H. et al. 2015. Incorporation of peptides targeting EGFR and FGFR1 into the adenoviral fibre knob domain, and their evaluation as targeted cancer therapies. Human Gene Therapy 26(5), pp. 320-329. (10.1089/hum.2015.015)
- Hsu, J. et al. 2015. Plasma membrane profiling defines an expanded class of cell surface proteins selectively targeted for degradation by HCMV US2 in cooperation with UL141. PLoS Pathogens 11(4), article number: e1004811. (10.1371/journal.ppat.1004811)
- Stack, G. et al. 2015. CD200 receptor restriction of myeloid cell responses antagonizes antiviral immunity and facilitates cytomegalovirus persistence within mucosal tissue. PLoS Pathogens 11(2), article number: e1004641. (10.1371/journal.ppat.1004641)
- Gabaev, I. et al. 2014. Expression of the human cytomegalovirus UL11 glycoprotein in viral infection and evaluation of its effect on virus-specific CD8 T cells. Journal of Virology 88(24), pp. 14326-14339. (10.1128/JVI.01691-14)
- Stanton, R. J. et al. 2014. HCMV pUL135 remodels the actin cytoskeleton to impair immune recognition of infected cells. Cell Host and Microbe 16(2), pp. 201-214. (10.1016/j.chom.2014.07.005)
- Weekes, M. et al. 2014. Quantitative temporal viromics: an approach to investigate host-pathogen interaction. Cell 157(6), pp. 1460-1472. (10.1016/j.cell.2014.04.028)
- Fielding, C. A. et al. 2014. Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation. PLoS Pathogens 10(5), article number: e1004058. (10.1371/journal.ppat.1004058)
- Aicheler, R., Wang, E., Tomasec, P., Wilkinson, G. W. G. and Stanton, R. J. 2013. Potential for natural killer cell-mediated antibody-dependent cellular cytotoxicity for control of human cytomegalovirus. Antibodies 2(4), pp. 617-635. (10.3390/antib2040617)
- Murrell, I., Tomasec, P., Wilkie, G. S., Dargan, D. J., Davison, A. J. and Stanton, R. J. 2013. Impact of sequence variation in the UL128 locus on production of human cytomegalovirus in fibroblast and epithelial cells. Journal of Virology 87(19), pp. 10489-10500. (10.1128/JVI.01546-13)
- Smith, W. et al. 2013. Human cytomegalovirus glycoprotein UL141 targets the TRAIL death receptors to thwart host innate antiviral defenses. Cell Host & Microbe 13(3), pp. 324-335. (10.1016/j.chom.2013.02.003)
- Fielding, C. A. et al. 2012. A novel human cytomegalovirus gene function targets the natural killer cell activating ligand MICA for lysosomal degradation [Abstract]. Immunology 137(S1), pp. 154. (10.1111/imm.12001)
- Prod'homme, V. et al. 2012. Human cytomegalovirus UL40 signal peptide regulates cell surface expression of the NK cell ligands HLA-E and gpUL18. The Journal of Immunology 188(6), pp. 2794-2804. (10.4049/jimmunol.1102068)
- Gabaev, I. et al. 2011. The human cytomegalovirus ul11 protein interacts with the receptor tyrosine phosphatase cd45, resulting in functional paralysis of t cells. PLoS Pathogens 7(12), article number: e1002432. (10.1371/journal.ppat.1002432)
- Stanton, R. J. et al. 2010. Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication. Journal of Clinical Investigation 120(9), pp. 3191-3208. (10.1172/JCI42955)
- Prod'homme, V. et al. 2010. Human cytomegalovirus UL141 promotes efficient downregulation of the natural killer cell activating ligand CD112. Journal of General Virology 91(8), pp. 2034-2039. (10.1099/vir.0.021931-0)
- Dargan, D. J. et al. 2010. Sequential mutations associated with adaptation of human cytomegalovirus to growth in cell culture. Journal of General Virology 91(6), pp. 1535-1546. (10.1099/vir.0.018994-0)
- Stanton, R. J., McSharry, B. P., Armstrong, M. L., Tomasec, P. and Wilkinson, G. W. G. 2008. Re-engineering adenovirus vector systems to enable high-throughput analyses of gene function. Biotechniques 45(6), pp. 659-668.
- McSharry, B. P. et al. 2008. Adenovirus E3/19K promotes evasion of NK cell recognition by intracellular sequestration of the NKG2D ligands major histocompatibility complex class I chain-related proteins A and B. Journal of Virology 82(9), pp. 4585-4594. (10.1128/JVI.02251-07)
- Wilkinson, G. W. G. et al. 2008. Modulation of natural killer cells by human cytomegalovirus. Journal of Clinical Virology 41(3), pp. 206-212. (10.1016/j.jcv.2007.10.027)
- Stanton, R. J., McSharry, B. P., Rickards, C. R., Wang, E. C. Y., Tomasec, P. and Wilkinson, G. W. G. 2007. Cytomegalovirus destruction of focal adhesions revealed in a high-throughput Western blot analysis of cellular protein expression. Journal of Virology 81(15), pp. 7860-7872. (10.1128/JVI.02247-06)
- Prod'homme, V. et al. 2007. The Human Cytomegalovirus MHC Class I Homolog UL18 Inhibits LIR-1+ but Activates LIR-1- NK Cells. The Journal of Immunology 178(7), pp. 4473-4481.
- Tomasec, P. et al. 2007. Adenovirus vector delivery stimulates natural killer cell recognition. Journal of General Virology 88(4), pp. 1103-1108. (10.1099/vir.0.82685-0)
- Stanton, R. J., Westmoreland, D., Fox, J. D., Davison, A. J. and Wilkinson, G. W. G. 2005. Stability of human cytomegalovirus genotypes in persistently infected renal transplant recipients. Journal of Medical Virology 75(1), pp. 42-46. (10.1002/jmv.20235)
- Stanton, R. J., Wilkinson, G. W. G. and Fox, J. D. 2003. Analysis of human herpesvirus-6 IE1 sequence variation in clinical samples. Journal of Medical Virology 71(4), pp. 578-584. (10.1002/jmv.10508)
Book sections
- Aicheler, R. J. and Stanton, R. J. 2013. Functional NK cell cytotoxicity assays against virus infected cells. In: Bailer, S. M. and Lieber, D. eds. Virus-Host Interactions., Vol. 1064. Methods in Molecular Biology Springer, pp. 275-287., (10.1007/978-1-62703-601-6_20)
Websites
- Nan, X. et al. 2022. VarLOCK - sequencing independent, rapid detection of SARS-CoV-2 variants of concern for point-of-care testing, qPCR pipelines and national wastewater surveillance. [Online]. medRxix: (10.1101/2022.01.06.21268555) Available at: https://www.medrxiv.org/content/10.1101/2022.01.06.21268555v1
- Clement, M. et al. 2021. IFITM3 regulates virus-induced inflammatory cytokine production by titrating Nogo-B orchestration of TLR responses. [Online]. bioRxiv. Available at: https://doi.org/10.1101/2021.07.23.453513
Research
My research relates to the biology, immunology and diagnosis of human cytomegalovirus (HCMV), as well as the development of recombinant Adenovirus vectors. Please see Viral Immunology, Cytomegalovirus and Adenovirus Virology and Oncolytic Adenovirus Virology for further details. You can also find publication information on ResearcherID, Researchgate and Google Scholar .
Teaching
I am the lead for Virology teaching within the Medical Degree. I lecture on Immunology and virology, and run virology practicals and tutorials. I also take students for laboratory projects at various points throughout the course. Within the intercalated BSc and Pharmacology degrees, I am an in-courser marker for student presentations of scientific papers, mark student presentations of their research projects, and run lab-based reseach projects. I also take students from other courses who wish to undertake a professional training year in a lab.
Within our Applied and Experimental Clinical Immunology MSc I teach on Virology, B-cell immunology, and Proteomics.
Biography
Career overview
- 2022-present: Head of Infection, Cardiff University School of Medicine
- 2021-present: Professor in Virology, Cardiff University School of Medicine
- 2018-2021: Reader in Microbiology, Cardiff University School of Medicine.
- 2015-2018: Senior Lecturer in Microbiology, Cardiff University School of Medicine.
- 2010-1015: Lecturer in Microbiology, Cardiff University School of Medicine.
- 2004-2010: Post-doctoral Researcher, Cardiff University School of Medicine
- 2003-2004: Post-doctoral Researcher, University of Wales College of Medicine
- 2002-2003: Research Assistant, University of Wales College of Medicine
- 1997-1998: Industrial Training Placement, Bacteriology, Public Health Laboratory Service
Education and Qualifications
- 1999-2002: PhD Virology, University of Wales College of Medicine
- 1999-2000: Diploma in Biomedical Methods, University of Wales College of Medicine
- 1995-1999: BSc (Hons) Microbiology, University of Wales College of Cardiff
Professional memberships
- Microbiology Society
- American Society for Microbiology
- British Society for Immunology
Committees and reviewing
- Member of the Wellcome Trust 'Discovery Award Advisory Group'
- Member of the Healthcare Research Wales Fellowship Grant committee
- Member of the 'Innovation to Impact' group
- Member of appraisal panels for 1st/2nd/3rd year transfers of PhD students within the institute of Infection & Immunity
- Editor for PNAS Nexus
- Editor for PLoS Pathogens
- Editor for Scientific Reports
- Editorial Board Member for the Journal of General Virology
Contact Details
Research themes
Specialisms
- Virology
- Immunology
