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Peter Watson

Professor Peter Watson

(he/him)

Users
Available for postgraduate supervision

Teams and roles for Peter Watson

  • Director for Postgraduate Education, Professor, Academic lead of imaging facilities, Postgraduate Research Teaching Co-ordinator

    School of Biosciences

Overview

Research overview

How mammalian cells regulate and spatially co-ordinate the process of moving cellular cargo, while ensuring that organelle homeostasis, is maintained and cargos are delivered correctly, is the focus of my research. Using small molecule inhibitors, toxins, and classical cell biology, and utilising both fluorescence and non-linear optical approaches, I study the pathways with which mammalian cells are able to move proteins,lipids and nanoparticles between cellular compartments.

Current lab members

Previous lab members

Undergraduate/Masters Students

  • Nathan Evans (Integrated Masters)
  • Tom Roach (Mres Project)
  • Danielle Keel (Integrated Masters)
  • Chris Grieve (Integrated Masters)
  • Daniel Lonergan (Integrated Masters)
  • Yasmeen Butt (Integrated Masters)
  • Tyler Davies (Integrated Masters)
  • Ghaith Al-Aisaee (Undergraduate Research Project)
  • Katie Lake (Undergraduate Research Project)
  • Rui Hart (Undergraduate Research Project)
  • Zoe Bannister (Undergraduate Research Project)
  • Bobbie Llewellyn (Undergraduate Research Project)
  • Duncan Carrol (Undergraduate Research Project)
  • Matthew Marchant (Undergraduate Research Project)
  • Maya Payne (Undergraduate Research Project)
  • Gemma Torien-Howie (Undergraduate Research Project)
  • Nathan Mielczarek (Undergraduate Research Project)
  • Dawid Grzech (Undergraduate Research Project)
  • Harry Jupe (Undergraduate Research Project)
  • Bethan Goodwin (Undergraduate Research Project)
  • Moses Tutesigensi (Undergraduate Research Project)
  • Rebecca Gundy (Undergraduate Research Project)
  • Jihua Xue (Undergraduate Research Project)
  • Katie Davis (Undergraduate Research Project)
  • Choonhoe Lum (Undergraduate Research Project)
  • Kirsty Lewis (Undergraduate Research Project)
  • James Thomas (Undergraduate Research Project)
  • Zoe Bassett (Biophotonics Research Project)
  • Michael Garhard (Biophotonics Research Project)
  • Ian Yekhlef  (Biophotonics Research Project)
  • Louise Barker (CUROP student)
  • Izzati Yussof (Summer student)
  • Chris Towers (Wellcome Trust Summer studentship)

Publication

2024

2023

2022

2021

2020

2019

2018

2017

2016

2015

2014

2013

2012

2011

2010

2009

2008

2006

2005

2004

2003

Articles

Book sections

Conferences

Monographs

Research

The compartmentalisation of mammalian cells allows the organisation of internal structures that have specific and distinct identity and function. Movement of components (proteins, lipids and solutes) between these structures is an ordered process, and occurs by the shuttling of membrane bound transport vesicles. Cargo is selectively incorporated into forming vesicles and targeted to their destination, where they fuse membranes with the acceptor compartment and deliver their cargo.

The machinery responsible for this targeted delivery needs to be returned to the original compartment to balance organelle homeostasis, and so these proteins are retrieved through a process of retrograde transport. Individual compartments are continually in a state of flux, and compartmental proteins and lipids are maintained through a balance of targeting, retention and retrieval. Components are continually moving between compartments, and it is the balance of traffic between them that defines the steady-state localisation of a molecule.

How mammalian cells regulate and spatially co-ordinate this process, to ensure that organelle homeostasis is maintained and cargo's are delivered correctly, is the focus of my research. I am also interested in developing novel microscopy techniques to allow the visualisation and quantification of intracellular structures.

CARS microscopy

In collaboration with Dr Paola Borri and Professor Wolfgang Langbein, we are utilising coherent anti-stokes Raman scattering microscopy to study lipid homeostasis within eukaryotic cells. CARS microscopy joins the chemical sensitivity and label-free noninvasiveness offered by vibrational spectroscopy with the inherent 3D sectioning capability of multiphoton microscopy. We have recently published our work on a method to perform frequency differential CARS (D-CARS) in Optics Letters.

Four Wave mixing imaging (FWM)

In collaboration with Dr Paola Borri and Professor Wolfgang Langbein, we have demonstrated a novel multiphoton microscope based on the detection of four wave mixing emitted from gold nanoparticles. This allows us to perform background free imaging of single gold labels of small sizes (down to 5nm) with sub-micrometer resolution. We have recently published this work in Optics Letters and are now investigating the applicability of this technique to the life sciences.

Microscopes

We have a number of microscopes within the lab available for use:

  • CRAM: Widefield fast timelapse configured for DAPI/FITC/TRITC/Cy5 fluorescence imaging and phase contrast for brightfield imaging.
  • Bernard: Widefield fast timelapse configured for DAPI/FITC/TRITC/Cy5 fluorescence imaging and phase contrast for brightfield imaging. Spinning disk microscope for DAPI/FITC/TRITC
  •  

We also have an eppendorf microinjection system that will fit on each of these microscopes to allow the microinjection, or microdelivery of material to the cell, or its local environment.

Collaborations

Cardiff University

  • Arwyn Jones
  • Ian Humphries
  • Mike Taylor
  • Paola Borri
  • Wolfgang Langbein
  • Dafydd Jones
  • Mark Gumbleton

COMPACT consortium

University of Bristol

University of Nottingham

University of Warwick

Teaching

Teaching

Module Lead for BI3253 - Advanced Cell Biology and Imaging. The aim of this module is to develop an advanced understanding of Cell Biology and the imaging methods utilised within the scientific field. It will address the major processes occurring within the cell (including membrane structure, function and transport, the endomembrane system, apoptosis, and cell death), and also expand into specialised cell biology systems (stem cells, diseased cells). The theory and application of advanced imaging technique will be addressed and students will develop an understanding of state of the art light and electron microscopy techniques. 

Contributor to BI4002 - Advanced Research Methods

Contributor to BIT002 - Research Techniques in Bioscience

Positions available

PhD

If you are interested in the fields of membrane trafficking, microscope development or drug delivery, and would like to be considered for a project within the lab send me an email with your background, research interests and current CV, or search for Cardiff projects on FindaPhD.com

Other positions

We are always interested in hosting self-funded positions within our research group, and there are a number of projects available in the fields of protein/lipid trafficking, drug delivery and microscope development. To discuss any of these projects, just get in touch.

Biography

I attained my first degree in Medical Biochemistry from the University of Birmingham, and gained my PhD under the supervision of Professor John Davey at the University of Warwick, studying the pheromone communication pathway of fission yeast. Staying at the University of Warwick, in 2000 I started studying toxin trafficking within the labs of Professors Mike Lord and Lynne Roberts, and during this time I was lucky enough to be trained in light microscopy by Dr. Jez Simpson and Dr. Rainer Pepperkok at EMBL in Heidelberg.

Towards the end of 2003 I moved to the lab of Dr. David Stephens at the University of Bristol, where I used both light and electron microscopy to look at how proteins exit the ER and are moved throughout the cell in membranous transport carriers.

In February 2007 I moved to the School of Biosciences at Cardiff University to take up an RCUK Fellowship in Translational Research in Experimental Medicine.

Supervisions

Current supervision

Rhod Thomas

Rhod Thomas

Nicole Slesiona

Nicole Slesiona

Chelsea Taylor Stevens Stevens

Chelsea Taylor Stevens Stevens

Contact Details

Specialisms

  • Biochemistry and cell biology
  • Fluorescence microscopy
  • Drug delivery
  • Drug Trafficking
Life-saving limpets?

Life-saving limpets?

02 November 2017