Overview
- I am deputy laboratory manager and tissue culture specialist managing the Tissue Culture facilities within the department of Oral and Biomedical Sciences.
- I provide training for all new staff, including safety inductions, ensuring consistency amd continuity across users.
- I manage health and safety requirements for the facilities ensuring, current legislation is followed.
- I ensure equipment is maintained to ensure the safety of users of the facilites.
Publication
2016
- Sadaghiani, L., Gleeson, H., Youde, S., Waddington, R., Lynch, C. and Sloan, A. 2016. Growth factor liberation and DPSCs response following dentine conditioning. Journal of Dental Research 95(11), pp. 1298-1307. (10.1177/0022034516653568)
- Young, F. I. et al. 2016. Clonal heterogeneity in the neuronal and glial differentiation of dental pulp stem/progenitor cells. Stem Cells International 2016, article number: 1290561. (10.1155/2016/1290561)
2009
- Waddington, R. J., Youde, S. J., Lee, C. P. and Sloan, A. J. 2009. Isolation of distinct progenitor stem cell populations from dental pulp. Cells Tissues Organs 189(1-4), pp. 268-274. (10.1159/000151447)
2008
- Roberts, H. C., Moseley, R., Sloan, A. J., Youde, S. J. and Waddington, R. J. 2008. Lipopolysaccharide alters decorin and biglycan synthesis in rat alveolar bone osteoblasts: Consequences for bone repair during periodontal disease. European Journal of Oral Sciences 116(3), pp. 207-216. (10.1111/j.1600-0722.2008.00535.x)
- Thomas, K. J., Smith, K. L., Youde, S. J., Evans, M., Fiander, A. N., Borysiewicz, L. K. and Man, S. T. 2008. HPV16 E629-38-specific T cells kill cervical carcinoma cells despite partial evasion of T-cell effector function. International Journal of Cancer 122(12), pp. 2791-2799. (10.1002/ijc.23475)
2006
- McCarthy, C., Youde, S. J. and Man, S. T. 2006. Definition of an HPV18/45 cross-reactive human T-cell epitope after DNA immunisation of HLA-A2/KB transgenic mice. International Journal of Cancer 118(10), pp. 2514-21. (10.1002/ijc.21643)
2005
- Youde, S. J., McCarthy, C. M., Thomas, K. J., Smith, K. L. and Man, S. T. 2005. Cross-typic specificity and immunotherapeutic potential of a human HPV16 E7-specific CTL line. International Journal of Cancer 114(4), pp. 606-12. (10.1002/ijc.20779)
- Hill, S. C. et al. 2005. Activation of CD40 in cervical carcinoma cells facilitates CTL responses and augments chemotherapy-induced apoptosis. Journal of Immunology 174(1), pp. 41-50.
2000
- Youde, S. J., Dunbar, P. R. R., Evans, E. M. L., Fiander, A. N., Borysiewicz, L. K., Cerundolo, V. and Man, S. T. 2000. Use of fluorogenic histocompatibility leukocyte antigen-A*0201/HPV 16 E7 peptide complexes to isolate rare human cytotoxic T-lymphocyte-recognizing endogenous human papillomavirus antigens. Cancer Research 60(2), pp. 365-371.
1998
- Youde, S. J., Mower, J., Moore, D. P. and Parkes, A. B. 1998. Stress protein expression in primary and immortalized cultures of human thyroid cells: A model system for the study of stress proteins in the pathogenesis of autoimmune thyroid disease. Cell Stress & Chaperones 3(2), pp. 89-93.
1996
- Youde, S. J. and Parkes, A. B. 1996. Stress-induced expression of a thyroid peroxidase-like protein in cultured human thyroid cells. Autoimmunity 23(4), pp. 279-282.
Erthyglau
- Sadaghiani, L., Gleeson, H., Youde, S., Waddington, R., Lynch, C. and Sloan, A. 2016. Growth factor liberation and DPSCs response following dentine conditioning. Journal of Dental Research 95(11), pp. 1298-1307. (10.1177/0022034516653568)
- Young, F. I. et al. 2016. Clonal heterogeneity in the neuronal and glial differentiation of dental pulp stem/progenitor cells. Stem Cells International 2016, article number: 1290561. (10.1155/2016/1290561)
- Waddington, R. J., Youde, S. J., Lee, C. P. and Sloan, A. J. 2009. Isolation of distinct progenitor stem cell populations from dental pulp. Cells Tissues Organs 189(1-4), pp. 268-274. (10.1159/000151447)
- Roberts, H. C., Moseley, R., Sloan, A. J., Youde, S. J. and Waddington, R. J. 2008. Lipopolysaccharide alters decorin and biglycan synthesis in rat alveolar bone osteoblasts: Consequences for bone repair during periodontal disease. European Journal of Oral Sciences 116(3), pp. 207-216. (10.1111/j.1600-0722.2008.00535.x)
- Thomas, K. J., Smith, K. L., Youde, S. J., Evans, M., Fiander, A. N., Borysiewicz, L. K. and Man, S. T. 2008. HPV16 E629-38-specific T cells kill cervical carcinoma cells despite partial evasion of T-cell effector function. International Journal of Cancer 122(12), pp. 2791-2799. (10.1002/ijc.23475)
- McCarthy, C., Youde, S. J. and Man, S. T. 2006. Definition of an HPV18/45 cross-reactive human T-cell epitope after DNA immunisation of HLA-A2/KB transgenic mice. International Journal of Cancer 118(10), pp. 2514-21. (10.1002/ijc.21643)
- Youde, S. J., McCarthy, C. M., Thomas, K. J., Smith, K. L. and Man, S. T. 2005. Cross-typic specificity and immunotherapeutic potential of a human HPV16 E7-specific CTL line. International Journal of Cancer 114(4), pp. 606-12. (10.1002/ijc.20779)
- Hill, S. C. et al. 2005. Activation of CD40 in cervical carcinoma cells facilitates CTL responses and augments chemotherapy-induced apoptosis. Journal of Immunology 174(1), pp. 41-50.
- Youde, S. J., Dunbar, P. R. R., Evans, E. M. L., Fiander, A. N., Borysiewicz, L. K., Cerundolo, V. and Man, S. T. 2000. Use of fluorogenic histocompatibility leukocyte antigen-A*0201/HPV 16 E7 peptide complexes to isolate rare human cytotoxic T-lymphocyte-recognizing endogenous human papillomavirus antigens. Cancer Research 60(2), pp. 365-371.
- Youde, S. J., Mower, J., Moore, D. P. and Parkes, A. B. 1998. Stress protein expression in primary and immortalized cultures of human thyroid cells: A model system for the study of stress proteins in the pathogenesis of autoimmune thyroid disease. Cell Stress & Chaperones 3(2), pp. 89-93.
- Youde, S. J. and Parkes, A. B. 1996. Stress-induced expression of a thyroid peroxidase-like protein in cultured human thyroid cells. Autoimmunity 23(4), pp. 279-282.
Research
- I provide technical expertise to carry out research project work including pilot studies, completing project work for publication and working independently on short – medium term projects.
- I undertake research for both internal and external funders.
Teaching
- I lecture on the Oral Biology and CITER (Cardiff Institute for Tissue Engineering and Repair) MSc courses run by the department.
- I provide practicals for the students undertaking these courses.
- I am currently undertaking the Fellow Development Scheme run by Cardiff University.
Biography
My current position is deputy laboratory manager and tissue culture specialist for the department of Oral and Biomedical Sciences in the Dental school.
Research and teaching
- I lecture on, and run practicals for the Oral Biology and the Cardiff Institute for Tissue Engineering and Repair (CITER) MSc courses run by the department.
- I undertake independent research, carrying out pilot studies for PI’s.
- I supervise students carrying out tissue culture projects.
- I maintain cultures for individuals who have a heavy workload or are away.
- I have created and maintain a cell bank of mycoplasma free cells.
- I am involved in Science in Health providing taster days to AS level students.
- I, along with other members of the technical team, provide laboratory based work experience for AS level students.
- I provide laboratory work experience for the Cardiff University programme Career Confident, formerly GOWales.
TC management
- Before starting work in tissue culture, all new departmental members’ and students undergo a TC induction, which involves an oral and practical component; the practical induction is carried out on a 1:1 basis.
- I have created the induction material and am responsible for keeping it up to date.
- I decide the competency of the new user ensuring they are safe to work alone.
- I provide ad hoc technical advice and support, detailed technical expertise such as planning experiments.
- I chair TC users group meetings.
- I am the point of contact for engineers regarding TC equipment.
Safety
- I write and update risk assessments and standard operating procedures for the tissue culture labs.
- I represent TC on the departmental health and safety committee.
- I carry out safety checks.
- I am deputy fire warden.
Team and department duties
- I maintain the inventory of all equipment within the department.
- I manage the departmental standard operating procedure and risk assessment data bases.
- I carry out ordering for the department as a whole using the Oracle software system.
University duties
· I am a member of the university Genetic Modification and Biological Agents Safety Committee.
· I am a member of the university Biobank review committee.
· I am a member of the team organising the Cardiff University Technical Staff Conference being held in June 2023.
Wider roles
· I am a member of the committee organizing the IST annual conference to be held in September 2023.
· I carry out public engagement activities via CITER in my role as a STEM Ambassador.
PhD
The role of stress proteins in the initiation of autoimmune thyroid disease. Cardiff University 1997.
Degree:
B.Sc. (Hons.) Biomedical Sciences I Class Cardiff University 1992.
PD
Honours and awards
- I graduated from the Herschel Programme for Women in Technical Leadership, 2022.
- I won the William O'Grady prize for technicians 2018, part of Cardiff University's Celebrating Excellence Awards.
- I was nominated for the Royal Society of Biology HE Bioscience Technician of the Year 2018.
Professional memberships
- I am a registered Chartered Scientist.
- I am a member of the IST.
Academic positions
Committees and reviewing
- I am a member of the committee organising the IST conference for 2023.
Supervisions
- I supervise both under gradaute and post graduate students whose projects include tissue culture.
Contact Details
+44 29225 12081
University Dental Hospital, Room 4.417, Heath Park, Cardiff, CF14 4XY
Research themes
Specialisms
- Stem cells
- cell culture