![Renata Jurkowska](https://profiles-cardiff.imgix.net/attachments/1086?w=200&h=200&auto=format&crop=faces&fit=crop&q=90")
Dr Renata Jurkowska
Darllenydd
Ysgol y Biowyddorau
- Ar gael fel goruchwyliwr ôl-raddedig
Trosolwyg
Sut gall yr un dilyniant genetig arwain at fwy na 200 o fathau o gelloedd a geir yn y corff dynol, pob un â morffoleg a swyddogaeth unigryw?
Rwyf bob amser wedi cael fy nghyfareddu gan brosesau gwahaniaethu cellog, sy'n dangos sut y gellir dehongli'r wyddor genetig yn gain gan ryngadwaith o brosesau epigenetig i yrru celloedd cynhenid tuag at eu brasterau arbenigol. Mae'r syndod hwn wedi ysgogi datblygiad fy niddordebau ymchwil o amgylch epigeneteg a gwahaniaethu bôn-gelloedd.
Fy niddordebau ymchwil hirdymor yw deall:
1) Sut mae gwahanol boblogaethau celloedd yr ysgyfaint dynol yn cydweithredu i ffurfio organ swyddogaethol
2) Sut mae rheoleiddio epigenetig yn gyrru hunaniaeth gellog yn yr ysgyfaint iach
3) Sut mae dadreoleiddio prosesau epigenetig oherwydd sarhad amgylcheddol, fel mwg sigaréts neu lygredd aer yn cyfrannu at ddatblygu clefydau'r ysgyfaint
Cyhoeddiad
2024
- Jurkowska, R. Z. 2024. Role of epigenetic mechanisms in the pathogenesis of chronic respiratory diseases and response to inhaled exposures: from basic concepts to clinical applications. Pharmacology & Therapeutics, article number: 108732. (10.1016/j.pharmthera.2024.108732)
2023
- Schwartz, U. et al. 2023. High-resolution transcriptomic and epigenetic profiling identifies novel regulators of COPD. The EMBO Journal 42, article number: e111272. (10.15252/embj.2022111272)
- Dordevic, M. et al. 2023. EpiCRISPR targeted methylation of Arx gene initiates transient switch of mouse pancreatic alpha to insulin-producing cells. Frontiers in Endocrinology 14, article number: 1134478. (10.3389/fendo.2023.1134478)
2022
- Pohl, S. T., Prada, M. L., Espinet, E. and Jurkowska, R. 2022. Practical considerations for complex tissue dissociation for single-cell transcriptomics. In: Calogero, R. and Benes, V. eds. Single Cell Transcriptomics. Methods in Molecular Biology., Vol. 2584. New York: Springer, pp. 371-387., (10.1007/978-1-0716-2756-3_19)
- Jurkowska, R. Z. and Jeltsch, A. 2022. Mechanisms and biological roles of DNA methyltransferases and DNA methylation: From past achievements to future challenges. In: Jeltsch, A. and Jurkowska, R. Z. eds. DNA Methyltransferases - Role and Function., Vol. 1389. Advances in Experimental Medicine and Biology Springer, pp. 1-19., (10.1007/978-3-031-11454-0_1)
- Jurkowska, R. Z. and Jeltsch, A. 2022. Enzymology of mammalian DNA methyltransferases. In: Jeltsch, A. and Jurkowska, R. Z. eds. DNA Methyltransferases - Role and Function., Vol. 1389. Advances in Experimental Medicine and Biology Springer, pp. 69-110., (10.1007/978-3-031-11454-0_4)
- Ravichandran, M. et al. 2022. Pronounced sequence specificity of the TET enzyme catalytic domain guides its cellular function. Science Advances 8(36) (10.1126/sciadv.abm2427)
2021
- Prada, M. L. et al. 2021. Versatile workflow for cell-type resolved transcriptional and epigenetic profiles from cryopreserved human lung. JCI Insight 6(5), article number: e140443. (10.1172/jci.insight.140443)
2020
- Al-Amri, M. and Jurkowska, R. eds. 2020. Proceedings of Cardiff Institute for Tissue Engineering & Repair annual scientific meeting (CITER ASM 2020). Cardiff: CITER & Cardiff University.
- Ogger, P. P., Silva, J. D., Aghapour, M., Persson, I. M., Tulen, C., Jurkowska, R. and Ubags, N. D. 2020. Early Career Members at the ERS Lung Science Conference 2020: metabolic alterations in lung ageing and disease. Breathe 16(3), article number: 200063. (10.1183/20734735.0063-2020)
- Gao, L. et al. 2020. Comprehensive structure-function characterization of DNMT3B and DNMT3A reveals distinctive de novo DNA methylation mechanisms. Nature Communications 11, article number: 3355. (10.1038/s41467-020-17109-4)
2019
- Serçin, ?. et al. 2019. A solid-phase transfection platform for arrayed CRISPR screens. Molecular Systems Biology 15(12), article number: e8983. (10.15252/msb.20198983)
2018
- Rajavelu, A. et al. 2018. Chromatin-dependent allosteric regulation of DNMT3A activity by MeCP2. Nucleic Acids Research 46(17), pp. 9044–9056. (10.1093/nar/gky715)
- Emperle, M., Dukatz, M., Kunert, S., Holzer, K., Rajavelu, A., Jurkowska, R. Z. and Jeltsch, A. 2018. The DNMT3A R882H mutation does not cause dominant negative effects in purified mixed DNMT3A/R882H complexes. Scientific Reports 8(1), pp. -., article number: 13242. (10.1038/s41598-018-31635-8)
- Emperle, M., Rajavelu, A., Kunert, S., Arimondo, P. B., Reinhardt, R., Jurkowska, R. Z. and Jeltsch, A. 2018. The DNMT3A R882H mutant displays altered flanking sequence preferences. Nucleic Acids Research 46(6), pp. 3130-3139. (10.1093/nar/gky168)
- Ravichandran, M., Jurkowska, R. and Jurkowski, T. 2018. Target specificity of mammalian DNA methylation and demethylation machinery. Organic and Biomolecular Chemistry(9), pp. 1419-1435. (10.1039/C7OB02574B)
2017
- Jurkowska, R. Z. et al. 2017. H3K14ac is linked to methylation of H3K9 by the triple Tudor domain of SETDB1. Nature Communications 8, article number: 2057. (10.1038/s41467-017-02259-9)
- Stepper, P. et al. 2017. Efficient targeted DNA methylation with chimeric dCas9-Dnmt3a-Dnmt3L methyltransferase. Nucleic Acids Research 45(4), pp. 1703-1713. (10.1093/nar/gkw1112)
2016
- Jurkowska, R. Z. and Jeltsch, A. 2016. Enzymology of mammalian DNA methyltransferases. Advances in Experimental Medicine and Biology 945, pp. 87-122. (10.1007/978-3-319-43624-1_5)
- Jurkowska, R. Z. and Jeltsch, A. 2016. Mechanisms and biological roles of DNA methyltransferases and DNA methylation: From past achievements to future challenges. Advances in Experimental Medicine and Biology 945, pp. 1-17. (10.1007/978-3-319-43624-1_1)
- Jeltsch, A. and Jurkowska, R. Z. 2016. Allosteric control of mammalian DNA methyltransferases - a new regulatory paradigm. Nucleic Acids Research 44(18), pp. 8556-8575. (10.1093/nar/gkw723)
2015
- Lungu, C., Muegge, K., Jeltsch, A. and Jurkowska, R. Z. 2015. An ATPase-deficient variant of the SNF2 family member HELLS shows altered dynamics at pericentromeric Heterochromatin. Journal of Molecular Biology 427(10), pp. 1903-1915. (10.1016/j.jmb.2015.03.014)
2014
- Kungulovski, G. et al. 2014. Application of histone modification-specific interaction domains as an alternative to antibodies. Genome Research 24(11), pp. 1842-1853. (10.1101/gr.170985.113)
- Emperle, M., Rajavelu, A., Reinhardt, R., Jurkowska, R. Z. and Jeltsch, A. 2014. Cooperative DNA binding and protein/DNA fiber formation increases the activity of the Dnmt3a DNA methyltransferase. Journal of Biological Chemistry 289(43), pp. 29602-29613. (10.1074/jbc.M114.572032)
- Jeltsch, A. and Jurkowska, R. Z. 2014. New concepts in DNA methylation. Trends in Biochemical Sciences 39(7), pp. 310-318. (10.1016/j.tibs.2014.05.002)
- Bashtrykov, P., Jankevicius, G., Jurkowska, R. Z., Ragozin, S. and Jeltsch, A. 2014. The UHRF1 protein stimulates the activity and specificity of the maintenance DNA methyltransferase DNMT1 by an allosteric mechanism. Journal of Biological Chemistry 289(7), pp. 4106-4116. (10.1074/jbc.M113.528893)
2013
- Jurkowska, R. Z. and Jeltsch, A. 2013. Genomic imprinting-the struggle of the genders at the molecular level. Angewandte Chemie International Edition 52(51), pp. 13524-13536. (10.1002/anie.201307005)
- Jeltsch, A. and Jurkowska, R. Z. 2013. Multimerization of the Dnmt3a DNA methyltransferase and its functional implications. Progress in Molecular Biology and Translational Science 117, pp. 445-464. (10.1016/B978-0-12-386931-9.00016-7)
2012
- Siddique, A. N. et al. 2012. Targeted methylation and gene silencing of VEGF-A in human cells by using a designed Dnmt3a-Dnmt3L single-chain fusion protein with increased DNA methylation activity. Journal of Molecular Biology 425(3), pp. 479—491. (10.1016/j.jmb.2012.11.038)
- Bashtrykov, P., Jankevicius, G., Smarandache, A., Jurkowska, R., Ragozin, S. and Jeltsch, A. 2012. Specificity of Dnmt1 for methylation of hemimethylated CpG sites resides in its catalytic domain. Chemistry and Biology 19(5), pp. 572-578. (10.1016/j.chembiol.2012.03.010)
- Halby, L. et al. 2012. Rapid synthesis of new DNMT inhibitors derivatives of procainamide. ChemBioChem 13(1), pp. 157-165. (10.1002/cbic.201100522)
2011
- Jurkowska, R. Z., Jurkowski, T. P. and Jeltsch, A. 2011. Structure and function of mammalian DNA methyltransferases. ChemBioChem 12(2), pp. 206—222. (10.1002/cbic.201000195)
- Jurkowska, R. Z., Ceccaldi, A., Zhang, Y., Arimondo, P. B. and Jeltsch, A. 2011. DNA methyltransferase assays. In: Epigenetics Protocols., Vol. 791. Methods in Molecular Biology Humana Press, pp. 157-177., (10.1007/978-1-61779-316-5_13)
- Jurkowska, R. Z. et al. 2011. Oligomerization and binding of the Dnmt3a DNA methyltransferase to parallel DNA molecules. Journal of Biological Chemistry 286(27), pp. 24200-24207. (10.1074/jbc.M111.254987)
- Jurkowska, R. Z., Siddique, A. N., Jurkowski, T. P. and Jeltsch, A. 2011. Approaches to enzyme and substrate design of the murine Dnmt3a DNA methyltransferase. ChemBioChem 12(10), pp. 1589—1594. (10.1002/cbic.201000673)
- Ceccaldi, A. et al. 2011. C5-DNA methyltransferase inhibitors: from screening to effects on zebrafish embryo development. ChemBioChem 12(9), pp. 1337-1345. (10.1002/cbic.201100130)
- Siddique, A. N., Jurkowska, R. Z., Jurkowski, T. P. and Jeltsch, A. 2011. Auto-methylation of the mouse DNA-(cytosine C5)-methyltransferase Dnmt3a at its active site cysteine residue. FEBS Journal 278(12), pp. 2055—2063. (10.1111/j.1742-4658.2011.08121.x)
- Rajavelu, A., Jurkowska, R. Z., Fritz, J. and Jeltsch, A. 2011. Function and disruption of DNA Methyltransferase 3a cooperative DNA binding and nucleoprotein filament formation. Nucleic Acids Research 40(2), pp. 569–580. (10.1093/nar/gkr753)
2010
- Champion, C. et al. 2010. Mechanistic insights on the inhibition of C5 DNA methyltransferases by zebularine. PLoS ONE 5(8), article number: e12388. (10.1371/journal.pone.0012388)
- Dhayalan, A., Rajavelu, A., Rathert, P., Tamas, R., Jurkowska, R. Z., Ragozin, S. and Jeltsch, A. 2010. The Dnmt3a PWWP domain reads histone 3 lysine 36 trimethylation and Guides DNA methylation. Journal of Biological Chemistry 285(34), pp. 26114-26120. (10.1074/jbc.M109.089433)
- Zhang, Y. et al. 2010. Chromatin methylation activity of Dnmt3a and Dnmt3a/3L is guided by interaction of the ADD domain with the histone H3 tail. Nucleic Acids Research 38(13), pp. 4246–4253. (10.1093/nar/gkq147)
- Jurkowska, R. Z. and Jeltsch, A. 2010. Silencing of gene expression by targeted DNA methylation: concepts and approaches. In: Engineered Zinc Finger Proteins., Vol. 649. Humana Press, pp. 149-161., (10.1007/978-1-60761-753-2_9)
- Jeltsch, A. and Jurkowska, R. Z. 2010. DNA methylation. In: The Chemical Biology of Nucleic Acids. Wiley, pp. 307-301., (10.1002/9780470664001.ch14)
- Métivier, R. et al. 2010. Erratum: Cyclical DNA methylation of a transcriptionally active promoter. Nature 463(7279), article number: 384. (10.1038/nature08661)
2008
- Jurkowska, R. Z. et al. 2008. Formation of nucleoprotein filaments by mammalian DNA methyltransferase Dnmt3a in complex with regulator Dnmt3L. Nucleic Acids Research 36(21), pp. 6656–6663. (10.1093/nar/gkn747)
- Rathert, P. et al. 2008. Protein lysine methyltransferase G9a acts on non-histone targets. Nature Chemical Biology 4(6), pp. 344–346. (10.1038/nchembio.88)
- Métivier, R. et al. 2008. Cyclical DNA methylation of a transcriptionally active promoter. Nature 452(7183), pp. 45–50. (10.1038/nature06544)
2007
- Jia, D., Jurkowska, R. Z., Zhang, X., Jeltsch, A. and Cheng, X. 2007. Structure of Dnmt3a bound to Dnmt3L suggests a model for de novo DNA methylation. Nature 449(7159), pp. 248–251. (10.1038/nature06146)
- Gallais, R. et al. 2007. Deoxyribonucleic acid methyl transferases 3a and 3b associate with the nuclear orphan receptor COUP-TFI during gene activation. Molecular Endocrinology -Baltimore- 21(9), pp. 2085–2098. (10.1210/me.2006-0490)
- Jeltsch, A., Jurkowska, R. Z., Jurkowski, T. P., Liebert, K., Rathert, P. and Schlickenrieder, M. 2007. Application of DNA methyltransferases in targeted DNA methylation. Applied Microbiology and Biotechnology 75(6), pp. 1233—1240. (10.1007/s00253-007-0966-0)
2006
- Gowher, H., Loutchanwoot, P., Vorobjeva, O., Handa, V., Jurkowska, R. Z., Jurkowski, T. P. and Jeltsch, A. 2006. Mutational analysis of the catalytic domain of the murine Dnmt3a DNA-(cytosine C5)-methyltransferase. Journal of Molecular Biology 357(3), pp. 928—941. (10.1016/j.jmb.2006.01.035)
Adrannau llyfrau
- Pohl, S. T., Prada, M. L., Espinet, E. and Jurkowska, R. 2022. Practical considerations for complex tissue dissociation for single-cell transcriptomics. In: Calogero, R. and Benes, V. eds. Single Cell Transcriptomics. Methods in Molecular Biology., Vol. 2584. New York: Springer, pp. 371-387., (10.1007/978-1-0716-2756-3_19)
- Jurkowska, R. Z. and Jeltsch, A. 2022. Mechanisms and biological roles of DNA methyltransferases and DNA methylation: From past achievements to future challenges. In: Jeltsch, A. and Jurkowska, R. Z. eds. DNA Methyltransferases - Role and Function., Vol. 1389. Advances in Experimental Medicine and Biology Springer, pp. 1-19., (10.1007/978-3-031-11454-0_1)
- Jurkowska, R. Z. and Jeltsch, A. 2022. Enzymology of mammalian DNA methyltransferases. In: Jeltsch, A. and Jurkowska, R. Z. eds. DNA Methyltransferases - Role and Function., Vol. 1389. Advances in Experimental Medicine and Biology Springer, pp. 69-110., (10.1007/978-3-031-11454-0_4)
- Jurkowska, R. Z., Ceccaldi, A., Zhang, Y., Arimondo, P. B. and Jeltsch, A. 2011. DNA methyltransferase assays. In: Epigenetics Protocols., Vol. 791. Methods in Molecular Biology Humana Press, pp. 157-177., (10.1007/978-1-61779-316-5_13)
- Jurkowska, R. Z. and Jeltsch, A. 2010. Silencing of gene expression by targeted DNA methylation: concepts and approaches. In: Engineered Zinc Finger Proteins., Vol. 649. Humana Press, pp. 149-161., (10.1007/978-1-60761-753-2_9)
- Jeltsch, A. and Jurkowska, R. Z. 2010. DNA methylation. In: The Chemical Biology of Nucleic Acids. Wiley, pp. 307-301., (10.1002/9780470664001.ch14)
Erthyglau
- Jurkowska, R. Z. 2024. Role of epigenetic mechanisms in the pathogenesis of chronic respiratory diseases and response to inhaled exposures: from basic concepts to clinical applications. Pharmacology & Therapeutics, article number: 108732. (10.1016/j.pharmthera.2024.108732)
- Schwartz, U. et al. 2023. High-resolution transcriptomic and epigenetic profiling identifies novel regulators of COPD. The EMBO Journal 42, article number: e111272. (10.15252/embj.2022111272)
- Dordevic, M. et al. 2023. EpiCRISPR targeted methylation of Arx gene initiates transient switch of mouse pancreatic alpha to insulin-producing cells. Frontiers in Endocrinology 14, article number: 1134478. (10.3389/fendo.2023.1134478)
- Ravichandran, M. et al. 2022. Pronounced sequence specificity of the TET enzyme catalytic domain guides its cellular function. Science Advances 8(36) (10.1126/sciadv.abm2427)
- Prada, M. L. et al. 2021. Versatile workflow for cell-type resolved transcriptional and epigenetic profiles from cryopreserved human lung. JCI Insight 6(5), article number: e140443. (10.1172/jci.insight.140443)
- Ogger, P. P., Silva, J. D., Aghapour, M., Persson, I. M., Tulen, C., Jurkowska, R. and Ubags, N. D. 2020. Early Career Members at the ERS Lung Science Conference 2020: metabolic alterations in lung ageing and disease. Breathe 16(3), article number: 200063. (10.1183/20734735.0063-2020)
- Gao, L. et al. 2020. Comprehensive structure-function characterization of DNMT3B and DNMT3A reveals distinctive de novo DNA methylation mechanisms. Nature Communications 11, article number: 3355. (10.1038/s41467-020-17109-4)
- Serçin, ?. et al. 2019. A solid-phase transfection platform for arrayed CRISPR screens. Molecular Systems Biology 15(12), article number: e8983. (10.15252/msb.20198983)
- Rajavelu, A. et al. 2018. Chromatin-dependent allosteric regulation of DNMT3A activity by MeCP2. Nucleic Acids Research 46(17), pp. 9044–9056. (10.1093/nar/gky715)
- Emperle, M., Dukatz, M., Kunert, S., Holzer, K., Rajavelu, A., Jurkowska, R. Z. and Jeltsch, A. 2018. The DNMT3A R882H mutation does not cause dominant negative effects in purified mixed DNMT3A/R882H complexes. Scientific Reports 8(1), pp. -., article number: 13242. (10.1038/s41598-018-31635-8)
- Emperle, M., Rajavelu, A., Kunert, S., Arimondo, P. B., Reinhardt, R., Jurkowska, R. Z. and Jeltsch, A. 2018. The DNMT3A R882H mutant displays altered flanking sequence preferences. Nucleic Acids Research 46(6), pp. 3130-3139. (10.1093/nar/gky168)
- Ravichandran, M., Jurkowska, R. and Jurkowski, T. 2018. Target specificity of mammalian DNA methylation and demethylation machinery. Organic and Biomolecular Chemistry(9), pp. 1419-1435. (10.1039/C7OB02574B)
- Jurkowska, R. Z. et al. 2017. H3K14ac is linked to methylation of H3K9 by the triple Tudor domain of SETDB1. Nature Communications 8, article number: 2057. (10.1038/s41467-017-02259-9)
- Stepper, P. et al. 2017. Efficient targeted DNA methylation with chimeric dCas9-Dnmt3a-Dnmt3L methyltransferase. Nucleic Acids Research 45(4), pp. 1703-1713. (10.1093/nar/gkw1112)
- Jurkowska, R. Z. and Jeltsch, A. 2016. Enzymology of mammalian DNA methyltransferases. Advances in Experimental Medicine and Biology 945, pp. 87-122. (10.1007/978-3-319-43624-1_5)
- Jurkowska, R. Z. and Jeltsch, A. 2016. Mechanisms and biological roles of DNA methyltransferases and DNA methylation: From past achievements to future challenges. Advances in Experimental Medicine and Biology 945, pp. 1-17. (10.1007/978-3-319-43624-1_1)
- Jeltsch, A. and Jurkowska, R. Z. 2016. Allosteric control of mammalian DNA methyltransferases - a new regulatory paradigm. Nucleic Acids Research 44(18), pp. 8556-8575. (10.1093/nar/gkw723)
- Lungu, C., Muegge, K., Jeltsch, A. and Jurkowska, R. Z. 2015. An ATPase-deficient variant of the SNF2 family member HELLS shows altered dynamics at pericentromeric Heterochromatin. Journal of Molecular Biology 427(10), pp. 1903-1915. (10.1016/j.jmb.2015.03.014)
- Kungulovski, G. et al. 2014. Application of histone modification-specific interaction domains as an alternative to antibodies. Genome Research 24(11), pp. 1842-1853. (10.1101/gr.170985.113)
- Emperle, M., Rajavelu, A., Reinhardt, R., Jurkowska, R. Z. and Jeltsch, A. 2014. Cooperative DNA binding and protein/DNA fiber formation increases the activity of the Dnmt3a DNA methyltransferase. Journal of Biological Chemistry 289(43), pp. 29602-29613. (10.1074/jbc.M114.572032)
- Jeltsch, A. and Jurkowska, R. Z. 2014. New concepts in DNA methylation. Trends in Biochemical Sciences 39(7), pp. 310-318. (10.1016/j.tibs.2014.05.002)
- Bashtrykov, P., Jankevicius, G., Jurkowska, R. Z., Ragozin, S. and Jeltsch, A. 2014. The UHRF1 protein stimulates the activity and specificity of the maintenance DNA methyltransferase DNMT1 by an allosteric mechanism. Journal of Biological Chemistry 289(7), pp. 4106-4116. (10.1074/jbc.M113.528893)
- Jurkowska, R. Z. and Jeltsch, A. 2013. Genomic imprinting-the struggle of the genders at the molecular level. Angewandte Chemie International Edition 52(51), pp. 13524-13536. (10.1002/anie.201307005)
- Jeltsch, A. and Jurkowska, R. Z. 2013. Multimerization of the Dnmt3a DNA methyltransferase and its functional implications. Progress in Molecular Biology and Translational Science 117, pp. 445-464. (10.1016/B978-0-12-386931-9.00016-7)
- Siddique, A. N. et al. 2012. Targeted methylation and gene silencing of VEGF-A in human cells by using a designed Dnmt3a-Dnmt3L single-chain fusion protein with increased DNA methylation activity. Journal of Molecular Biology 425(3), pp. 479—491. (10.1016/j.jmb.2012.11.038)
- Bashtrykov, P., Jankevicius, G., Smarandache, A., Jurkowska, R., Ragozin, S. and Jeltsch, A. 2012. Specificity of Dnmt1 for methylation of hemimethylated CpG sites resides in its catalytic domain. Chemistry and Biology 19(5), pp. 572-578. (10.1016/j.chembiol.2012.03.010)
- Halby, L. et al. 2012. Rapid synthesis of new DNMT inhibitors derivatives of procainamide. ChemBioChem 13(1), pp. 157-165. (10.1002/cbic.201100522)
- Jurkowska, R. Z., Jurkowski, T. P. and Jeltsch, A. 2011. Structure and function of mammalian DNA methyltransferases. ChemBioChem 12(2), pp. 206—222. (10.1002/cbic.201000195)
- Jurkowska, R. Z. et al. 2011. Oligomerization and binding of the Dnmt3a DNA methyltransferase to parallel DNA molecules. Journal of Biological Chemistry 286(27), pp. 24200-24207. (10.1074/jbc.M111.254987)
- Jurkowska, R. Z., Siddique, A. N., Jurkowski, T. P. and Jeltsch, A. 2011. Approaches to enzyme and substrate design of the murine Dnmt3a DNA methyltransferase. ChemBioChem 12(10), pp. 1589—1594. (10.1002/cbic.201000673)
- Ceccaldi, A. et al. 2011. C5-DNA methyltransferase inhibitors: from screening to effects on zebrafish embryo development. ChemBioChem 12(9), pp. 1337-1345. (10.1002/cbic.201100130)
- Siddique, A. N., Jurkowska, R. Z., Jurkowski, T. P. and Jeltsch, A. 2011. Auto-methylation of the mouse DNA-(cytosine C5)-methyltransferase Dnmt3a at its active site cysteine residue. FEBS Journal 278(12), pp. 2055—2063. (10.1111/j.1742-4658.2011.08121.x)
- Rajavelu, A., Jurkowska, R. Z., Fritz, J. and Jeltsch, A. 2011. Function and disruption of DNA Methyltransferase 3a cooperative DNA binding and nucleoprotein filament formation. Nucleic Acids Research 40(2), pp. 569–580. (10.1093/nar/gkr753)
- Champion, C. et al. 2010. Mechanistic insights on the inhibition of C5 DNA methyltransferases by zebularine. PLoS ONE 5(8), article number: e12388. (10.1371/journal.pone.0012388)
- Dhayalan, A., Rajavelu, A., Rathert, P., Tamas, R., Jurkowska, R. Z., Ragozin, S. and Jeltsch, A. 2010. The Dnmt3a PWWP domain reads histone 3 lysine 36 trimethylation and Guides DNA methylation. Journal of Biological Chemistry 285(34), pp. 26114-26120. (10.1074/jbc.M109.089433)
- Zhang, Y. et al. 2010. Chromatin methylation activity of Dnmt3a and Dnmt3a/3L is guided by interaction of the ADD domain with the histone H3 tail. Nucleic Acids Research 38(13), pp. 4246–4253. (10.1093/nar/gkq147)
- Métivier, R. et al. 2010. Erratum: Cyclical DNA methylation of a transcriptionally active promoter. Nature 463(7279), article number: 384. (10.1038/nature08661)
- Jurkowska, R. Z. et al. 2008. Formation of nucleoprotein filaments by mammalian DNA methyltransferase Dnmt3a in complex with regulator Dnmt3L. Nucleic Acids Research 36(21), pp. 6656–6663. (10.1093/nar/gkn747)
- Rathert, P. et al. 2008. Protein lysine methyltransferase G9a acts on non-histone targets. Nature Chemical Biology 4(6), pp. 344–346. (10.1038/nchembio.88)
- Métivier, R. et al. 2008. Cyclical DNA methylation of a transcriptionally active promoter. Nature 452(7183), pp. 45–50. (10.1038/nature06544)
- Jia, D., Jurkowska, R. Z., Zhang, X., Jeltsch, A. and Cheng, X. 2007. Structure of Dnmt3a bound to Dnmt3L suggests a model for de novo DNA methylation. Nature 449(7159), pp. 248–251. (10.1038/nature06146)
- Gallais, R. et al. 2007. Deoxyribonucleic acid methyl transferases 3a and 3b associate with the nuclear orphan receptor COUP-TFI during gene activation. Molecular Endocrinology -Baltimore- 21(9), pp. 2085–2098. (10.1210/me.2006-0490)
- Jeltsch, A., Jurkowska, R. Z., Jurkowski, T. P., Liebert, K., Rathert, P. and Schlickenrieder, M. 2007. Application of DNA methyltransferases in targeted DNA methylation. Applied Microbiology and Biotechnology 75(6), pp. 1233—1240. (10.1007/s00253-007-0966-0)
- Gowher, H., Loutchanwoot, P., Vorobjeva, O., Handa, V., Jurkowska, R. Z., Jurkowski, T. P. and Jeltsch, A. 2006. Mutational analysis of the catalytic domain of the murine Dnmt3a DNA-(cytosine C5)-methyltransferase. Journal of Molecular Biology 357(3), pp. 928—941. (10.1016/j.jmb.2006.01.035)
Llyfrau
- Al-Amri, M. and Jurkowska, R. eds. 2020. Proceedings of Cardiff Institute for Tissue Engineering & Repair annual scientific meeting (CITER ASM 2020). Cardiff: CITER & Cardiff University.
Ymchwil
Mae'r ysgyfaint dynol wedi'i adeiladu gan fwy na 50 math o gelloedd, sy'n cydweithredu i greu organ hardd sy'n hanfodol i ni anadlu. Yn feirniadol, mae diffyg gallu adfywiol yn nodwedd allweddol o lawer o glefydau'r ysgyfaint a heneiddio. Felly, mae deall prosesau moleciwlaidd sy'n ofynnol ar gyfer adfywio'r ysgyfaint yn hanfodol bwysig ar gyfer ymchwil biolegol.
Mae ein grŵp yn ymchwilio i sut mae rheoleiddio epigenetig yn gyrru hunaniaeth gellog yn yr ysgyfaint dynol gyda'r nod o ddarganfod ymyriadau therapiwtig newydd ar gyfer adfywio'r ysgyfaint.
Er mwyn datblygu triniaethau newydd, mae angen i ni ddeall sut mae unigolion iach yn datblygu clefydau'r ysgyfaint a nodi targedau addas ar gyfer datblygu cyffuriau. Mae addasiadau epigenetig yn grwpiau cemegol ar ein gwybodaeth enetig sy'n pennu pa enynnau sy'n weithredol a pha rai sydd wedi'u cau. Maent yn cael eu dysregulated gan amlygiad amgylcheddol sy'n achosi clefydau. Yn gyffrous, gellir eu trin hefyd gyda'r potensial i wella clefydau. Felly, mae signalau epigenetig yn darparu lefel reoleiddio gyffrous a heb ei archwilio i raddau helaeth ar gyfer nodi digwyddiadau sy'n gyrru clefydau, rheoleiddwyr clefydau newydd, a biofarcwyr .
Rydym yn defnyddio profion epigenomig ledled genomau a dulliau omeg un gell ar y cyd ag offer golygu epigenetig seiliedig ar CRISPR a modelau organoid 3D, i hyrwyddo dealltwriaeth fiolegol gwahaniaethu celloedd yr ysgyfaint a nodi biofarcwyr epigenetig a strategaethau therapiwtig newydd ar gyfer cleifion â chlefydau anadlol cronig.
Addysgu
cyfrannwr modiwl: Ffisioleg BI2331
cyfrannwr modiwl: Cysyniadau Clefyd BI2332
cyfrannwr modiwl: Genynnau BI3254 Genynnau i Genomau
Contrributor: Chwyldro omeg BI3252
Bywgraffiad
Ers mis Medi 2019 rwyf wedi bod yn Uwch-ddarlithydd yn yr Is-adran Biofeddygaeth yn Ysgol y Biowyddorau ym Mhrifysgol Caerdydd, lle mae gen i'r pleser o weithio mewn amgylchedd ymchwil hynod gydweithredol ac ysbrydoledig. Yn ddiweddar, rwyf wedi sicrhau Cymrodoriaeth Arweinwyr y Dyfodol UKRI, a ddechreuais ym mis Mehefin 2024.
Ar ôl cwblhau fy BSc ac MSc mewn Biotechnoleg ym Mhrifysgol Warsaw yng Ngwlad Pwyl, ymunais â'r grŵp o Athro Albert Jeltsch (Prifysgol Jacobs Bremen, yr Almaen) ar gyfer astudiaethau PhD mewn Biocemeg, a ddaeth i ben gyda rhagoriaeth arbennig. Ymchwiliais i briodweddau ensymatig a rheoleiddio methyltransferases DNA dynol, ensymau allweddol sy'n ymwneud â hunaniaeth a swyddogaeth gellog. Sbardunodd fy PhD ddiddordeb hir mewn epigeneteg a chrisialu fy niddordebau ymchwil o amgylch gwahaniaethu bôn-gelloedd ac adfywio meinweoedd. Yn ystod fy amser ôl-ddoethurol (ym Mhrifysgol Jacobs Bremen i ddechrau, yr Almaen ac yna ym Mhrifysgol Stuttgart, yr Almaen), ymchwiliais i fecanweithiau gwahanol ddosbarthiadau o ensymau epigenetig i ddeall eu swyddogaeth a'u cyfraniad at glefydau dynol.
Rwy'n hoffi archwilio meysydd sy'n dod i'r amlwg a chymryd rhan mewn prosiectau rhyngddisgyblaethol. Felly, ar ôl fy postdoc, newidiais fy maes ymchwil o fiocemeg i glefydau'r ysgyfaint a'r sector ymchwil o'r byd academaidd i gwmni newydd. O ddiwedd 2015 tan fis Gorffennaf 2019, roeddwn yn arweinydd grŵp o'r tîm ymchwil Epigenetics a COPD yng Nghanolfan Arloesi BioMed X yn Heidelberg (yr Almaen), sefydliad sy'n hyrwyddo model cydweithredu newydd ar y rhyngwyneb rhwng academia a diwydiant. Yno, lluniais a chyflawnais brosiect ymchwil arloesol a gynhaliwyd mewn cydweithrediad â'r diwydiant fferyllfa, a oedd yn archwilio rheoleiddio epigenetig clefyd rhwystrol cronig yr ysgyfaint i ddarparu llwybrau therapiwtig newydd ar gyfer y clefyd hwn ar yr ysgyfaint na ellir ei wella. Dangoswyd llwyddiant y prosiect a'i effaith trwy gaffael yr holl ganlyniadau gan y partner pharma ar gyfer parhad mewnol.
Anrhydeddau a dyfarniadau
- Cymrodoriaeth Arweinwyr y Dyfodol UKRI (2024)
- Cymrawd yr Academi Addysg Uwch (FHEA) (2023)
- Gwobr Academi Gwyddorau Meddygol Springboard (2022)
- Gwobr Geoffrey Laurent am y Cyflwyniad Llafar Gorau yng Nghynhadledd Gwyddorau'r Ysgyfaint, Portiwgal (2024 a 2020)
- Gwobr Rhaglen Dyfodol Caerdydd (2021)
- Gwobr Rhaglen Crucible Cymru (2021)
- Cymrodoriaeth ôl-ddoethurol a grant ymchwil a ddyfarnwyd gan Sefydliad Carl Zeiss (2015)
- Gwobr cymrodoriaeth tymor byr EMBO (2014)
- Gwobr poster gorau yng nghyfarfod a gweithdy 6ed NEB, Bremen, Yr Almaen (2010)
- Ysgoloriaeth ar gyfer cyflawniadau gwyddonol nodedig gan Weinyddiaeth Addysg Gwlad Pwyl (2004-2003 a 2003-2002)
- Ysgoloriaeth a roddwyd ar gyfer cyflawniadau nodedig, Prifysgol Warsaw, Gwlad Pwyl (2004-2000)
Aelodaethau proffesiynol
- Aelod o Gymdeithas Ymchwil yr Ysgyfaint Prydain (BALR) (o 2019) ac aelod o bwyllgor gwaith BALR (o 2023)
- Aelod o Bwyllgor Adolygu'r Gymdeithas Ryngwladol ar gyfer Epigenetig Moleciwlaidd a Chlinigol (isMOCLEP) (o 2022)
- Aelod o Gymdeithas Thorasig Cymru (o 2021) ac aelod o bwyllgor gwaith WTS (2022)
- Aelod o'r Gymdeithas Biocemegol (o 2019)
- Aelod o'r Gymdeithas Resbiradol Ewropeaidd (o 2016)
Safleoedd academaidd blaenorol
- Mehefin 2024 - presennol: Cymrawd Arweinwyr y Dyfodol UKRI
- 2019 - presennol: Uwch Ddarlithydd, Prifysgol Caerdydd
- 2015 - 2019: Arweinydd Prosiect a Grŵp yng Nghanolfan Arloesi BioMed X (Heidelberg, yr Almaen)
- 2012 - 2019: Ymchwilydd Ôl-ddoethurol, Prifysgol Stuttgart (Yr Almaen)
- 2009 - 2011: Ymchwilydd Ôl-ddoethurol, Prifysgol Jakobs Bremen (Yr Almaen)
Ymrwymiadau siarad cyhoeddus
Enghreifftiau dethol o wahoddiadau siaradwr diweddar:
- Cyngres Cymdeithas Resbiradol Ewrop, Fienna, Awstria (2024)
- Cymdeithas Thorasig Americanaidd, San Diego, UDA (2024)
- Sgwrs seminar yn MTWC ar gyfer Ffibrosing Cyfres Seminarau'r Clefyd yr Ysgyfaint, Coleg Imperial Llundain, DU (2024)
- Cyfarfod Cymdeithas Ymchwil yr Ysgyfaint Prydain, Dundee, UK (2024)
- Sgwrs seminar yn Wellcome-Wolfson Institute for Experimental Medicine, Belfast, Gogledd Irland (2024)
- Fforwm Llawryfog y Byd, Shanghai, China (enwebiad o Academi'r Gwyddorau Meddygol) (2024)
- Sgwrs seminar yn Sefydliad Maeth ac Iechyd Shanghai, Academi Gwyddorau Tsieineaidd, Tsieina (2024)
- Cyfarfod Rhyngwladol Cymdeithas Epigeneteg, Rhufain, DU (2023)
- Cyfarfod Cymdeithas Thorasig Prydain, Llundain, DU (2023)
- Cyfarfod Cymdeithas Thorasig Cymru, Caerdydd, DU (2023)
- Cyflwyniad seminar siaradwr ar gyfer rhwydwaith COPDiNET (ar-lein) (2023)
Pwyllgorau ac adolygu
- Aelod o Bwll Arbenigwyr BBSRC, panel Gwobr Tirwedd Ddoethurol BBSRC a phanel Pwyllgor Modd Ymatebol BBSRC C (o fis Ionawr 2024)
- Aelod o Goleg Adolygu Cymheiriaid UKRI Talent ac adolygydd Cynllun Cymrodoriaeth Arweinwyr y Dyfodol UKRI (o 2023)
- Adolygydd grant ar gyfer UKRI, Ymddiriedolaeth Croeso, Sefydliad Gwyddoniaeth Gwlad Pwyl a Sefydliad Gwyddoniaeth Ffrainc
- Aelod o Fwrdd Golygyddol Epigenetig Clinigol (o 2022), Cyfathrebu Epigenetig (o 2022) ac Epigenomes (o 2018)
- adolygydd cyfnodolion amrywiol, gan gynnwys Nucleic Acid Research, European Respiratory Journal, Nature Reviews Molecular Cell Biology, Science Advances, Clinical Epigenetics and Chromatin and Epigenetics.
Meysydd goruchwyliaeth
Mae gen i ddiddordeb mewn goruchwylio myfyrwyr PhD ym meysydd:
- epigenetics
- gwahaniaethu bôn-gelloedd
- bioleg yr ysgyfaint
- Clefydau cronig yr ysgyfaint
- Datblygu technolegau proffilio epigenomig newydd
Aelodau cyfredol Grŵp Epigeneteg yr Ysgyfaint:
Dr Stephanie Pohl (PDRA)
Petar Popov (myfyriwr PhD / Cydymaith Addysgu)
Pheobe Ross (myfyriwr PhD)
Megan Nicholson (myfyrwraig MPhil)
Laura Sear (Myfyriwr Meistr Integredig)
Aelodau Grŵp Epigeneteg yr ysgyfaint yn y gorffennol:
Dr Xinsheng Nan (PDRA)
Renjiao Li (myfyriwr PhD gwadd o Tsieina)
Stephanie Pohl (myfyriwr PhD)
Isaac Al-Moosawi (myfyriwr MRes)
Diana Stoian (myfyriwr MRes)
Alex Titimeaua (myfyriwr MRes)
Alice Pike (Myfyriwr Meistr Integredig)
Jacob Hill (Myfyriwr Meistr Integredig)
Amelia Nathan (Myfyriwr Meistr Integredig)
Deborah Ackesson (Myfyriwr Meistr Integredig)
Simran Tac (myfyriwr MSc mewn Peirianneg Meinwe a Meddygaeth Adfywiol)
Arthur Pasanen-Zentz (myfyriwr MSc mewn Peirianneg Meinwe a Meddygaeth Adfywiol)
Contact Details
JurkowskaR@caerdydd.ac.uk
+44 29208 79067
Adeilad Syr Martin Evans, Rhodfa'r Amgueddfa, Caerdydd, CF10 3AX
+44 29208 79067
Adeilad Syr Martin Evans, Rhodfa'r Amgueddfa, Caerdydd, CF10 3AX
Themâu ymchwil
Arbenigeddau
- Epigeneteg
- Afiechydon anadlol
- Heneiddio
